UniProtKB - Q9H2G4 (TSYL2_HUMAN)
Protein
Testis-specific Y-encoded-like protein 2
Gene
TSPYL2
Organism
Homo sapiens (Human)
Status
Functioni
Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.2 Publications
Miscellaneous
Synaptic activity down-regulates TSPYL2 protein levels by inducing rapid proteasomal degradation.By similarity
Subject to X inactivation.
GO - Molecular functioni
- chromatin binding Source: GO_Central
- histone binding Source: GO_Central
- rDNA binding Source: UniProtKB
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- IRE1-mediated unfolded protein response Source: Reactome
- negative regulation of cell cycle Source: UniProtKB
- negative regulation of cell growth Source: UniProtKB
- negative regulation of DNA replication Source: UniProtKB
- nucleosome assembly Source: InterPro
- regulation of protein kinase activity Source: UniProtKB
- regulation of signal transduction Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator |
Biological process | Cell cycle, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9H2G4 |
Reactomei | R-HSA-381038, XBP1(S) activates chaperone genes |
SIGNORi | Q9H2G4 |
Names & Taxonomyi
Protein namesi | Recommended name: Testis-specific Y-encoded-like protein 2Short name: TSPY-like protein 2 Alternative name(s): Cell division autoantigen 1 Cutaneous T-cell lymphoma-associated antigen se20-4 Short name: CTCL-associated antigen se20-4 Differentially-expressed nucleolar TGF-beta1 target protein Nuclear protein of 79 kDa Short name: NP79 |
Gene namesi | Name:TSPYL2 Synonyms:CDA1, DENTT, TSPX ORF Names:HRIHFB2216 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000184205.14 |
HGNCi | HGNC:24358, TSPYL2 |
MIMi | 300564, gene |
neXtProti | NX_Q9H2G4 |
Subcellular locationi
Nucleus
- nucleolus Source: UniProtKB
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 20 | S → A: Impairs effect on cell proliferation; when associated with A-340. 1 Publication | 1 | |
Mutagenesisi | 340 | T → A: Impairs effect on cell proliferation; when associated with A-20. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 64061 |
OpenTargetsi | ENSG00000184205 |
PharmGKBi | PA134930188 |
Miscellaneous databases
Pharosi | Q9H2G4, Tbio |
Polymorphism and mutation databases
BioMutai | TSPYL2 |
DMDMi | 74752604 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289100 | 1 – 693 | Testis-specific Y-encoded-like protein 2Add BLAST | 693 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 11 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 20 | Phosphoserine1 Publication | 1 | |
Cross-linki | 163 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 165 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 340 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 658 | PhosphoserineBy similarity | 1 | |
Modified residuei | 668 | PhosphoserineBy similarity | 1 | |
Modified residuei | 671 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation.1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9H2G4 |
jPOSTi | Q9H2G4 |
MassIVEi | Q9H2G4 |
MaxQBi | Q9H2G4 |
PaxDbi | Q9H2G4 |
PeptideAtlasi | Q9H2G4 |
PRIDEi | Q9H2G4 |
ProteomicsDBi | 80546 |
PTM databases
iPTMneti | Q9H2G4 |
PhosphoSitePlusi | Q9H2G4 |
Expressioni
Tissue specificityi
Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas.4 Publications
Inductioni
Up-regulated in growth-arrested T-cells. Induced by TGFB1 and all-trans retinoic acid (ATRA) in lung cancer cells (at protein level).3 Publications
Gene expression databases
Bgeei | ENSG00000184205, Expressed in frontal cortex and 238 other tissues |
ExpressionAtlasi | Q9H2G4, baseline and differential |
Genevisiblei | Q9H2G4, HS |
Organism-specific databases
HPAi | ENSG00000184205, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Interacts with histones.
Interacts with CASK.
Part of a complex containing CASK, TBR1 and TSPYL2 (By similarity).
By similarityBinary interactionsi
Hide detailsQ9H2G4
GO - Molecular functioni
- histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 122034, 66 interactors |
IntActi | Q9H2G4, 56 interactors |
STRINGi | 9606.ENSP00000364591 |
Miscellaneous databases
RNActi | Q9H2G4, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 4 – 75 | Pro-richAdd BLAST | 72 | |
Compositional biasi | 180 – 211 | Arg-richAdd BLAST | 32 | |
Compositional biasi | 473 – 542 | Asn-richAdd BLAST | 70 | |
Compositional biasi | 557 – 675 | Asp-richAdd BLAST | 119 |
Sequence similaritiesi
Belongs to the nucleosome assembly protein (NAP) family.Curated
Phylogenomic databases
eggNOGi | KOG1508, Eukaryota |
GeneTreei | ENSGT00940000162496 |
HOGENOMi | CLU_025445_0_0_1 |
InParanoidi | Q9H2G4 |
OMAi | PNGWANP |
OrthoDBi | 1191764at2759 |
PhylomeDBi | Q9H2G4 |
TreeFami | TF313386 |
Family and domain databases
InterProi | View protein in InterPro IPR037231, NAP-like_sf IPR002164, NAP_family |
PANTHERi | PTHR11875, PTHR11875, 1 hit |
Pfami | View protein in Pfam PF00956, NAP, 1 hit |
SUPFAMi | SSF143113, SSF143113, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9H2G4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE
60 70 80 90 100
ETEAAQVLAD MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST
110 120 130 140 150
IESGYGEAPP PTESLEALPT PEASGGSLEI DFQVVQSSSF GGEGALETCS
160 170 180 190 200
AVGWAPQRLV DPKSKEEAII IVEDEDEDER ESMRSSRRRR RRRRRKQRKV
210 220 230 240 250
KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL KRKFIQMRRP
260 270 280 290 300
FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR
310 320 330 340 350
HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP
360 370 380 390 400
QARRHGNQDA SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG
410 420 430 440 450
SRIKRKKQEM KKRKTRGRCE VVIMEDAPDY YAVEDIFSEI SDIDETIHDI
460 470 480 490 500
KISDFMETTD YFETTDNEIT DINENICDSE NPDHNEVPNN ETTDNNESAD
510 520 530 540 550
DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG NNFFKGGFWG
560 570 580 590 600
SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD
610 620 630 640 650
RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE
660 670 680 690
GNYEEEGSED VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KST2 | J3KST2_HUMAN | Testis-specific Y-encoded-like prot... | TSPYL2 | 123 | Annotation score: | ||
J3KS33 | J3KS33_HUMAN | Testis-specific Y-encoded-like prot... | TSPYL2 | 250 | Annotation score: |
Sequence cautioni
The sequence AAG53596 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 442 | D → N in AAG53596 (PubMed:11318608).Curated | 1 | |
Sequence conflicti | 566 | D → V in AAG53596 (PubMed:11318608).Curated | 1 | |
Sequence conflicti | 609 | V → G in BAA34802 (PubMed:9853615).Curated | 1 | |
Sequence conflicti | 615 | K → R in BAA34802 (PubMed:9853615).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_075536 | 262 | I → M Found in patients with mild intellectual disability, unknown pathological significance. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF273046 mRNA Translation: AAG34906.1 AY040871 mRNA Translation: AAK72407.1 AL713652 mRNA Translation: CAD28461.1 BX322635 Genomic DNA No translation available. BC001566 mRNA Translation: AAH01566.1 BC024270 mRNA Translation: AAH24270.1 AF254794 mRNA Translation: AAG53596.1 Different initiation. AB015345 mRNA Translation: BAA34802.1 |
CCDSi | CCDS14350.1 |
RefSeqi | NP_071400.1, NM_022117.3 |
Genome annotation databases
Ensembli | ENST00000375442; ENSP00000364591; ENSG00000184205 |
GeneIDi | 64061 |
KEGGi | hsa:64061 |
UCSCi | uc004drw.4, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF273046 mRNA Translation: AAG34906.1 AY040871 mRNA Translation: AAK72407.1 AL713652 mRNA Translation: CAD28461.1 BX322635 Genomic DNA No translation available. BC001566 mRNA Translation: AAH01566.1 BC024270 mRNA Translation: AAH24270.1 AF254794 mRNA Translation: AAG53596.1 Different initiation. AB015345 mRNA Translation: BAA34802.1 |
CCDSi | CCDS14350.1 |
RefSeqi | NP_071400.1, NM_022117.3 |
3D structure databases
SMRi | Q9H2G4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122034, 66 interactors |
IntActi | Q9H2G4, 56 interactors |
STRINGi | 9606.ENSP00000364591 |
PTM databases
iPTMneti | Q9H2G4 |
PhosphoSitePlusi | Q9H2G4 |
Polymorphism and mutation databases
BioMutai | TSPYL2 |
DMDMi | 74752604 |
Proteomic databases
EPDi | Q9H2G4 |
jPOSTi | Q9H2G4 |
MassIVEi | Q9H2G4 |
MaxQBi | Q9H2G4 |
PaxDbi | Q9H2G4 |
PeptideAtlasi | Q9H2G4 |
PRIDEi | Q9H2G4 |
ProteomicsDBi | 80546 |
Protocols and materials databases
Antibodypediai | 12456, 238 antibodies |
DNASUi | 64061 |
Genome annotation databases
Ensembli | ENST00000375442; ENSP00000364591; ENSG00000184205 |
GeneIDi | 64061 |
KEGGi | hsa:64061 |
UCSCi | uc004drw.4, human |
Organism-specific databases
CTDi | 64061 |
DisGeNETi | 64061 |
EuPathDBi | HostDB:ENSG00000184205.14 |
GeneCardsi | TSPYL2 |
HGNCi | HGNC:24358, TSPYL2 |
HPAi | ENSG00000184205, Tissue enhanced (brain) |
MIMi | 300564, gene |
neXtProti | NX_Q9H2G4 |
OpenTargetsi | ENSG00000184205 |
PharmGKBi | PA134930188 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1508, Eukaryota |
GeneTreei | ENSGT00940000162496 |
HOGENOMi | CLU_025445_0_0_1 |
InParanoidi | Q9H2G4 |
OMAi | PNGWANP |
OrthoDBi | 1191764at2759 |
PhylomeDBi | Q9H2G4 |
TreeFami | TF313386 |
Enzyme and pathway databases
PathwayCommonsi | Q9H2G4 |
Reactomei | R-HSA-381038, XBP1(S) activates chaperone genes |
SIGNORi | Q9H2G4 |
Miscellaneous databases
BioGRID-ORCSi | 64061, 5 hits in 477 CRISPR screens |
ChiTaRSi | TSPYL2, human |
GeneWikii | TSPYL2 |
GenomeRNAii | 64061 |
Pharosi | Q9H2G4, Tbio |
PROi | PR:Q9H2G4 |
RNActi | Q9H2G4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000184205, Expressed in frontal cortex and 238 other tissues |
ExpressionAtlasi | Q9H2G4, baseline and differential |
Genevisiblei | Q9H2G4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR037231, NAP-like_sf IPR002164, NAP_family |
PANTHERi | PTHR11875, PTHR11875, 1 hit |
Pfami | View protein in Pfam PF00956, NAP, 1 hit |
SUPFAMi | SSF143113, SSF143113, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TSYL2_HUMAN | |
Accessioni | Q9H2G4Primary (citable) accession number: Q9H2G4 Secondary accession number(s): O94799, Q96DG7, Q9BZW6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations