MED28

Functionⁱ

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.1 Publication

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of smooth muscle cell differentiation Source: Ensembl
regulation of transcription, DNA-templated Source: UniProtKB-KW
stem cell population maintenance Source: Ensembl
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Actin-binding, Activator
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1989781 PPARA activates gene expression R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
SIGNORⁱ	Q9H204

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mediator of RNA polymerase II transcription subunit 28 Alternative name(s): Endothelial-derived protein 1 Mediator complex subunit 28 Merlin and Grb2-interacting cytoskeletal protein Short name: Magicin Tumor angiogenesis marker EG-1
Gene namesⁱ	Name:MED28 Synonyms:EG1 ORF Names:FKSG20
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 4

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000118579.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:24628 MED28
MIMⁱ	610311 gene
neXtProtⁱ	NX_Q9H204

Keywords - Cellular componentⁱ

Cytoplasm, Membrane, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	80306
Open Targets More...OpenTargetsⁱ	ENSG00000118579
PharmGKBⁱ	PA134935853

Polymorphism and mutation databases

BioMutaⁱ	MED28
DMDMⁱ	59799838

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000113981	1 – 178	Mediator of RNA polymerase II transcription subunit 28Add BLAST		178

Proteomic databases

EPDⁱ	Q9H204
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9H204
PaxDbⁱ	Q9H204
PeptideAtlas More...PeptideAtlasⁱ	Q9H204
PRIDEⁱ	Q9H204
ProteomicsDBⁱ	80465

PTM databases

iPTMnetⁱ	Q9H204
PhosphoSitePlusⁱ	Q9H204

Expressionⁱ

Tissue specificityⁱ

Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.1 Publication

Inductionⁱ

Up-regulated by endothelial cells when exposed to tumor conditional media.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000118579
CleanExⁱ	HS_MED28
ExpressionAtlasⁱ	Q9H204 baseline and differential
Genevisibleⁱ	Q9H204 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA035900 HPA035901

HPAⁱ

HPA035900
HPA035901

Interactionⁱ

Subunit structureⁱ

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
	P03255	2	EBI-514199,EBI-2603114	From Human adenovirus C serotype 5.
FYN	P06241	6	EBI-514199,EBI-515315
GRB2	P62993	3	EBI-514199,EBI-401755
LCK	P06239	4	EBI-514199,EBI-1348
MAGI3	Q5TCQ9	2	EBI-514199,EBI-310506
Med11	Q9D8C6	2	EBI-514199,EBI-6260909	From Mus musculus.
MED22	Q15528	2	EBI-514199,EBI-394687
MED29	Q9NX70	3	EBI-514199,EBI-394656
MED30	Q96HR3	4	EBI-514199,EBI-394659
Med30	Q9CQI9	2	EBI-514199,EBI-309220	From Mus musculus.
Med8	Q9D7W5	2	EBI-514199,EBI-7990252	From Mus musculus.
NF2	P35240	4	EBI-514199,EBI-1014472
NF2	P35240-1	2	EBI-514199,EBI-1014500
PRMT6	Q96LA8	2	EBI-514199,EBI-912440
PUF60	Q9UHX1	6	EBI-514199,EBI-1053259
SPERT	Q8NA61	3	EBI-514199,EBI-741724
SRC	P12931	3	EBI-514199,EBI-621482
YES1	P07947	2	EBI-514199,EBI-515331

Show more details

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	123219, 86 interactors
ComplexPortalⁱ	CPX-3227 Core mediator complex
CORUMⁱ	Q9H204
IntActⁱ	Q9H204, 59 interactors
Molecular INTeraction database More...MINTⁱ	Q9H204
STRINGⁱ	9606.ENSP00000237380

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9H204
SMRⁱ	Q9H204
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	109 – 145	Sequence analysisAdd BLAST		37

Sequence similaritiesⁱ

Belongs to the Mediator complex subunit 28 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	ENOG410IETV Eukaryota ENOG4111HUA LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006192
HOGENOMⁱ	HOG000113540
HOVERGENⁱ	HBG052448
InParanoidⁱ	Q9H204
KEGG Orthology (KO) More...KOⁱ	K15141
PhylomeDBⁱ	Q9H204
TreeFamⁱ	TF326988

Family and domain databases

InterProⁱ	View protein in InterPro IPR021640 Mediator_Med28
PANTHERⁱ	PTHR13512 PTHR13512, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9H204-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES 
        60         70         80         90        100
SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL 
       110        120        130        140        150
QLSVQKPEQV IKEDVSELRN ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH 
       160        170 
KKPADIPQGS LAYLEQASAN IPAPLKPT

Length:178

Mass (Da):19,520

Last modified:March 1, 2001 - v1

Checksum:ⁱ63DE023972908066

GO

Sequence cautionⁱ

The sequence AAK11563 differs from that shown. Reason: Frameshift at position 165.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF321617 mRNA Translation: AAG38612.1 AF358829 mRNA Translation: AAK32724.1 AF318059 mRNA Translation: AAL26906.1 AF317680 mRNA Translation: AAK11563.1 Frameshift. BC011936 mRNA Translation: AAH11936.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33963.1
NCBI Reference Sequences More...RefSeqⁱ	NP_079481.2, NM_025205.4
UniGeneⁱ	Hs.731966

Genome annotation databases

Ensemblⁱ	ENST00000237380; ENSP00000237380; ENSG00000118579
GeneIDⁱ	80306
KEGGⁱ	hsa:80306
UCSC genome browser More...UCSCⁱ	uc003gpk.2 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q9H204	MED28 isoform 1		PANTR		178
MED28 isoform 1		PONAB		178
Mediator complex subunit 28		GORGO		178
Uncharacterized protein		NOMLE		223

Protein	Similar proteins		Species	Score	Length
Q9H204	MED28 isoform 1		PANTR		178
MED28 isoform 1		PONAB		178
Mediator complex subunit 28		GORGO		178
Uncharacterized protein		NOMLE		223
Mediator complex subunit 28		PAPAN		191
+7

Protein	Similar proteins		Species	Score	Length
Q9H204	MED28 isoform 1		PANTR		178
MED28 isoform 1		PONAB		178
Mediator complex subunit 28		GORGO		178
Uncharacterized protein		NOMLE		223
Mediator complex subunit 28		PAPAN		191
+111

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF321617 mRNA Translation: AAG38612.1 AF358829 mRNA Translation: AAK32724.1 AF318059 mRNA Translation: AAL26906.1 AF317680 mRNA Translation: AAK11563.1 Frameshift. BC011936 mRNA Translation: AAH11936.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33963.1
NCBI Reference Sequences More...RefSeqⁱ	NP_079481.2, NM_025205.4
UniGeneⁱ	Hs.731966

3D structure databases

ProteinModelPortalⁱ	Q9H204
SMRⁱ	Q9H204
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	123219, 86 interactors
ComplexPortalⁱ	CPX-3227 Core mediator complex
CORUMⁱ	Q9H204
IntActⁱ	Q9H204, 59 interactors
Molecular INTeraction database More...MINTⁱ	Q9H204
STRINGⁱ	9606.ENSP00000237380

PTM databases

iPTMnetⁱ	Q9H204
PhosphoSitePlusⁱ	Q9H204

Polymorphism and mutation databases

BioMutaⁱ	MED28
DMDMⁱ	59799838

Proteomic databases

EPDⁱ	Q9H204
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9H204
PaxDbⁱ	Q9H204
PeptideAtlas More...PeptideAtlasⁱ	Q9H204
PRIDEⁱ	Q9H204
ProteomicsDBⁱ	80465

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	80306
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000237380; ENSP00000237380; ENSG00000118579
GeneIDⁱ	80306
KEGGⁱ	hsa:80306
UCSC genome browser More...UCSCⁱ	uc003gpk.2 human

Organism-specific databases

CTDⁱ	80306
DisGeNET More...DisGeNETⁱ	80306
EuPathDBⁱ	HostDB:ENSG00000118579.11
GeneCardsⁱ	MED28
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:24628 MED28
Human Protein Atlas More...HPAⁱ	HPA035900 HPA035901
MIMⁱ	610311 gene
neXtProtⁱ	NX_Q9H204
Open Targets More...OpenTargetsⁱ	ENSG00000118579
PharmGKBⁱ	PA134935853
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IETV Eukaryota ENOG4111HUA LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006192
HOGENOMⁱ	HOG000113540
HOVERGENⁱ	HBG052448
InParanoidⁱ	Q9H204
KEGG Orthology (KO) More...KOⁱ	K15141
PhylomeDBⁱ	Q9H204
TreeFamⁱ	TF326988

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1989781 PPARA activates gene expression R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
SIGNORⁱ	Q9H204

Miscellaneous databases

ChiTaRSⁱ	MED28 human
GeneWikiⁱ	MED28
GenomeRNAiⁱ	80306
Protein Ontology More...PROⁱ	PR:Q9H204
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000118579
CleanExⁱ	HS_MED28
ExpressionAtlasⁱ	Q9H204 baseline and differential
Genevisibleⁱ	Q9H204 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR021640 Mediator_Med28
PANTHERⁱ	PTHR13512 PTHR13512, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MED28_HUMAN
Accessionⁱ	Q9H204Primary (citable) accession number: Q9H204 Secondary accession number(s): Q9BZJ5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
	Last sequence update:	March 1, 2001
	Last modified:	July 18, 2018
	This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9H204 (MED28_HUMAN)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Mediator of RNA polymerase II transcription subunit 28

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytoskeleton

Nucleus

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ