UniProtKB - Q9H204 (MED28_HUMAN)
Mediator of RNA polymerase II transcription subunit 28
MED28
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
1 PublicationGO - Molecular functioni
- actin binding Source: UniProtKB-KW
GO - Biological processi
- negative regulation of smooth muscle cell differentiation Source: Ensembl
- positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
- positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
- RNA polymerase II preinitiation complex assembly Source: ComplexPortal
- stem cell population maintenance Source: Ensembl
Keywordsi
Molecular function | Actin-binding, Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9H204 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
SignaLinki | Q9H204 |
SIGNORi | Q9H204 |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 28Alternative name(s): Endothelial-derived protein 1 Mediator complex subunit 28 Merlin and Grb2-interacting cytoskeletal protein Short name: Magicin Tumor angiogenesis marker EG-1 |
Gene namesi | Name:MED28 Synonyms:EG1 ORF Names:FKSG20 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24628, MED28 |
MIMi | 610311, gene |
neXtProti | NX_Q9H204 |
VEuPathDBi | HostDB:ENSG00000118579 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- Nucleus 1 Publication
Other locations
- Membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.
Cytoskeleton
- cortical actin cytoskeleton Source: Ensembl
Nucleus
- core mediator complex Source: ComplexPortal
- mediator complex Source: GO_Central
- nucleoplasm Source: HPA
- nucleus Source: ComplexPortal
Other locations
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 80306 |
OpenTargetsi | ENSG00000118579 |
PharmGKBi | PA134935853 |
Miscellaneous databases
Pharosi | Q9H204, Tbio |
Genetic variation databases
BioMutai | MED28 |
DMDMi | 59799838 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000113981 | 1 – 178 | Mediator of RNA polymerase II transcription subunit 28Add BLAST | 178 |
Proteomic databases
EPDi | Q9H204 |
jPOSTi | Q9H204 |
MassIVEi | Q9H204 |
MaxQBi | Q9H204 |
PaxDbi | Q9H204 |
PeptideAtlasi | Q9H204 |
PRIDEi | Q9H204 |
ProteomicsDBi | 80465 |
PTM databases
iPTMneti | Q9H204 |
PhosphoSitePlusi | Q9H204 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000118579, Expressed in metanephros and 240 other tissues |
ExpressionAtlasi | Q9H204, baseline and differential |
Genevisiblei | Q9H204, HS |
Organism-specific databases
HPAi | ENSG00000118579, Low tissue specificity |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
Forms a ternary complex with NF2/merlin and GRB2. Binds to actin.
2 PublicationsBinary interactionsi
Q9H204
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 123219, 111 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q9H204 |
IntActi | Q9H204, 77 interactors |
MINTi | Q9H204 |
STRINGi | 9606.ENSP00000237380 |
Miscellaneous databases
RNActi | Q9H204, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9H204 |
SMRi | Q9H204 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 109 – 145 | Sequence analysisAdd BLAST | 37 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QSN9, Eukaryota |
GeneTreei | ENSGT00390000006192 |
InParanoidi | Q9H204 |
OMAi | MQPSPQH |
OrthoDBi | 1504193at2759 |
PhylomeDBi | Q9H204 |
TreeFami | TF326988 |
Family and domain databases
InterProi | View protein in InterPro IPR021640, Mediator_Med28 |
PANTHERi | PTHR13512, PTHR13512, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES
60 70 80 90 100
SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL
110 120 130 140 150
QLSVQKPEQV IKEDVSELRN ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH
160 170
KKPADIPQGS LAYLEQASAN IPAPLKPT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0YAA8 | H0YAA8_HUMAN | Mediator of RNA polymerase II trans... | MED28 | 179 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF321617 mRNA Translation: AAG38612.1 AF358829 mRNA Translation: AAK32724.1 AF318059 mRNA Translation: AAL26906.1 AF317680 mRNA Translation: AAK11563.1 Frameshift. BC011936 mRNA Translation: AAH11936.1 |
CCDSi | CCDS33963.1 |
RefSeqi | NP_079481.2, NM_025205.4 |
Genome annotation databases
Ensembli | ENST00000237380.12; ENSP00000237380.6; ENSG00000118579.13 |
GeneIDi | 80306 |
KEGGi | hsa:80306 |
MANE-Selecti | ENST00000237380.12; ENSP00000237380.6; NM_025205.5; NP_079481.2 |
UCSCi | uc003gpk.2, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF321617 mRNA Translation: AAG38612.1 AF358829 mRNA Translation: AAK32724.1 AF318059 mRNA Translation: AAL26906.1 AF317680 mRNA Translation: AAK11563.1 Frameshift. BC011936 mRNA Translation: AAH11936.1 |
CCDSi | CCDS33963.1 |
RefSeqi | NP_079481.2, NM_025205.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7EMF | electron microscopy | 3.50 | 1 | 1-178 | [»] | |
7ENA | electron microscopy | 4.07 | e | 1-178 | [»] | |
7ENC | electron microscopy | 4.13 | e | 1-178 | [»] | |
7ENJ | electron microscopy | 4.40 | 1 | 1-178 | [»] | |
7LBM | electron microscopy | 4.80 | o | 1-178 | [»] | |
7NVR | electron microscopy | 4.50 | q | 1-178 | [»] | |
AlphaFoldDBi | Q9H204 | |||||
SMRi | Q9H204 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123219, 111 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q9H204 |
IntActi | Q9H204, 77 interactors |
MINTi | Q9H204 |
STRINGi | 9606.ENSP00000237380 |
PTM databases
iPTMneti | Q9H204 |
PhosphoSitePlusi | Q9H204 |
Genetic variation databases
BioMutai | MED28 |
DMDMi | 59799838 |
Proteomic databases
EPDi | Q9H204 |
jPOSTi | Q9H204 |
MassIVEi | Q9H204 |
MaxQBi | Q9H204 |
PaxDbi | Q9H204 |
PeptideAtlasi | Q9H204 |
PRIDEi | Q9H204 |
ProteomicsDBi | 80465 |
Protocols and materials databases
Antibodypediai | 23086, 168 antibodies from 28 providers |
DNASUi | 80306 |
Genome annotation databases
Ensembli | ENST00000237380.12; ENSP00000237380.6; ENSG00000118579.13 |
GeneIDi | 80306 |
KEGGi | hsa:80306 |
MANE-Selecti | ENST00000237380.12; ENSP00000237380.6; NM_025205.5; NP_079481.2 |
UCSCi | uc003gpk.2, human |
Organism-specific databases
CTDi | 80306 |
DisGeNETi | 80306 |
GeneCardsi | MED28 |
HGNCi | HGNC:24628, MED28 |
HPAi | ENSG00000118579, Low tissue specificity |
MIMi | 610311, gene |
neXtProti | NX_Q9H204 |
OpenTargetsi | ENSG00000118579 |
PharmGKBi | PA134935853 |
VEuPathDBi | HostDB:ENSG00000118579 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSN9, Eukaryota |
GeneTreei | ENSGT00390000006192 |
InParanoidi | Q9H204 |
OMAi | MQPSPQH |
OrthoDBi | 1504193at2759 |
PhylomeDBi | Q9H204 |
TreeFami | TF326988 |
Enzyme and pathway databases
PathwayCommonsi | Q9H204 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
SignaLinki | Q9H204 |
SIGNORi | Q9H204 |
Miscellaneous databases
BioGRID-ORCSi | 80306, 707 hits in 1099 CRISPR screens |
ChiTaRSi | MED28, human |
GeneWikii | MED28 |
GenomeRNAii | 80306 |
Pharosi | Q9H204, Tbio |
PROi | PR:Q9H204 |
RNActi | Q9H204, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000118579, Expressed in metanephros and 240 other tissues |
ExpressionAtlasi | Q9H204, baseline and differential |
Genevisiblei | Q9H204, HS |
Family and domain databases
InterProi | View protein in InterPro IPR021640, Mediator_Med28 |
PANTHERi | PTHR13512, PTHR13512, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MED28_HUMAN | |
Accessioni | Q9H204Primary (citable) accession number: Q9H204 Secondary accession number(s): Q9BZJ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families