UniProtKB - Q9H1Y0 (ATG5_HUMAN)
Autophagy protein 5
ATG5
Functioni
GO - Biological processi
- aggrephagy Source: ParkinsonsUK-UCL
- antigen processing and presentation of endogenous antigen Source: Ensembl
- apoptotic process Source: UniProtKB-KW
- autophagosome assembly Source: UniProtKB
- autophagy Source: UniProtKB
- autophagy of mitochondrion Source: GO_Central
- autophagy of nucleus Source: GO_Central
- blood vessel remodeling Source: Ensembl
- cellular response to nitrogen starvation Source: GO_Central
- cellular response to nitrosative stress Source: Ensembl
- C-terminal protein lipidation Source: GO_Central
- heart contraction Source: Ensembl
- macroautophagy Source: Reactome
- negative regulation of apoptotic process Source: Ensembl
- negative regulation of cell death Source: MGI
- negative regulation of histone H4-K16 acetylation Source: Ensembl
- negative regulation of phagocytosis Source: Ensembl
- negative regulation of protein ubiquitination Source: Ensembl
- negative regulation of reactive oxygen species metabolic process Source: Ensembl
- negative stranded viral RNA replication Source: Ensembl
- negative thymic T cell selection Source: Ensembl
- otolith development Source: Ensembl
- positive regulation by symbiont of host autophagy Source: UniProtKB
- positive regulation of mucus secretion Source: Ensembl
- post-translational protein modification Source: UniProtKB
- protein lipidation involved in autophagosome assembly Source: Ensembl
- regulation of cilium assembly Source: UniProtKB
- regulation of cytokine production involved in immune response Source: Ensembl
- regulation of release of sequestered calcium ion into cytosol Source: Ensembl
- response to drug Source: Ensembl
- response to fungus Source: Ensembl
- vasodilation Source: Ensembl
- ventricular cardiac muscle cell development Source: Ensembl
Keywordsi
Biological process | Apoptosis, Autophagy, Host-virus interaction, Immunity |
Enzyme and pathway databases
PathwayCommonsi | Q9H1Y0 |
Reactomei | R-HSA-1632852, Macroautophagy R-HSA-5205685, PINK1-PRKN Mediated Mitophagy R-HSA-8934903, Receptor Mediated Mitophagy R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling |
SIGNORi | Q9H1Y0 |
Names & Taxonomyi
Protein namesi | Recommended name: Autophagy protein 5Alternative name(s): APG5-like Apoptosis-specific protein |
Gene namesi | Name:ATG5 Synonyms:APG5L, ASP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:589, ATG5 |
MIMi | 604261, gene |
neXtProti | NX_Q9H1Y0 |
VEuPathDBi | HostDB:ENSG00000057663.12 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
Note: Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.By similarity
Cytoskeleton
- axoneme Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Other locations
- Atg12-Atg5-Atg16 complex Source: GO_Central
- autophagosome Source: MGI
- cytoplasm Source: UniProtKB
- membrane Source: MGI
- mitochondria-associated endoplasmic reticulum membrane Source: Ensembl
- phagocytic vesicle membrane Source: Reactome
- phagophore assembly site membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Involvement in diseasei
Spinocerebellar ataxia, autosomal recessive, 25 (SCAR25)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_079274 | 122 | E → D in SCAR25; reduced conjugation to ATG12; decrease in autophagy activity. 1 PublicationCorresponds to variant dbSNP:rs1131692265EnsemblClinVar. | 1 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 130 | K → R: Loss of conjugaction with ATG12. Does affect interaction with DHX58, nor with MAVS. 2 Publications | 1 |
Keywords - Diseasei
Disease variant, NeurodegenerationOrganism-specific databases
DisGeNETi | 9474 |
MalaCardsi | ATG5 |
MIMi | 617584, phenotype |
OpenTargetsi | ENSG00000057663 |
PharmGKBi | PA24880 |
Miscellaneous databases
Pharosi | Q9H1Y0, Tbio |
Genetic variation databases
BioMutai | ATG5 |
DMDMi | 17366828 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218994 | 1 – 275 | Autophagy protein 5Add BLAST | 275 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Cross-linki | 130 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)Curated |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q9H1Y0 |
jPOSTi | Q9H1Y0 |
MassIVEi | Q9H1Y0 |
MaxQBi | Q9H1Y0 |
PaxDbi | Q9H1Y0 |
PeptideAtlasi | Q9H1Y0 |
PRIDEi | Q9H1Y0 |
ProteomicsDBi | 80456 [Q9H1Y0-1] 80457 [Q9H1Y0-2] |
PTM databases
iPTMneti | Q9H1Y0 |
PhosphoSitePlusi | Q9H1Y0 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000057663, Expressed in colonic mucosa and 236 other tissues |
ExpressionAtlasi | Q9H1Y0, baseline and differential |
Genevisiblei | Q9H1Y0, HS |
Organism-specific databases
HPAi | ENSG00000057663, Low tissue specificity |
Interactioni
Subunit structurei
Forms a conjugate with ATG12 (PubMed:12207896, PubMed:11825910, PubMed:17709747, PubMed:26812546). The ATG5-ATG12 conjugate forms a complex with several units of ATG16L1.
Forms an 800-kDa complex composed of ATG12-ATG5 and ATG16L2 (By similarity).
Interacts with TECPR1; the interaction is direct and does not take place when ATG16L1 is associated with the ATG5-ATG12 conjugate (PubMed:21575909, PubMed:22342342).
Interacts with DHX58/RIG-1, IFIH1/MDA5 and MAVS/IPS-1 in monomeric form as well as in ATG12-ATG5 conjugate form. The interaction with MAVS is further enhanced upon vesicular stomatitis virus (VSV) infection (PubMed:17709747).
Interacts with ATG3 (PubMed:12207896, PubMed:11825910).
Interacts with ATG7 and ATG10 (By similarity).
Interacts with FADD (PubMed:15778222).
Interacts with Bassoon/BSN; this interaction is important for the regulation of presynaptic autophagy (By similarity).
Interacts with ATG16L2 (By similarity).
By similarity7 Publications(Microbial infection) Interacts transiently interacts with hepatitis C virus (HCV) protein NS5B during HCV infection.
1 PublicationBinary interactionsi
Hide detailsQ9H1Y0
Protein-protein interaction databases
BioGRIDi | 114859, 66 interactors |
ComplexPortali | CPX-200, ATG12-ATG5-ATG16L1 complex CPX-354, ATG12-ATG5-ATG16L2 complex CPX-356, ATG5-ATG12 complex CPX-358, ATG5-ATG12-TECPR1 complex |
DIPi | DIP-29758N |
IntActi | Q9H1Y0, 55 interactors |
MINTi | Q9H1Y0 |
STRINGi | 9606.ENSP00000358072 |
Miscellaneous databases
RNActi | Q9H1Y0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9H1Y0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2976, Eukaryota |
GeneTreei | ENSGT00390000004766 |
HOGENOMi | CLU_051894_1_0_1 |
InParanoidi | Q9H1Y0 |
OMAi | MVPRLSY |
PhylomeDBi | Q9H1Y0 |
TreeFami | TF314415 |
Family and domain databases
Gene3Di | 1.10.246.190, 1 hit 3.10.20.620, 1 hit |
InterProi | View protein in InterPro IPR007239, Atg5 IPR042526, Atg5_HR IPR042527, Atg5_UblA_dom |
PANTHERi | PTHR13040, PTHR13040, 1 hit |
Pfami | View protein in Pfam PF04106, APG5, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD
60 70 80 90 100
KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI
110 120 130 140 150
TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH
160 170 180 190 200
KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ
210 220 230 240 250
KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP
260 270
LQWLSEHLSY PDNFLHISII PQPTD
The sequence of this isoform differs from the canonical sequence as follows:
1-79: MTDDKDVLRD...FEYEGTPLKW → M
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GV54 | A0A1B0GV54_HUMAN | Autophagy protein 5 | ATG5 | 153 | Annotation score: | ||
A0A1B0GUS1 | A0A1B0GUS1_HUMAN | Autophagy protein 5 | ATG5 | 159 | Annotation score: | ||
A0A2R8Y718 | A0A2R8Y718_HUMAN | Autophagy protein 5 | ATG5 | 233 | Annotation score: | ||
L7UQJ2 | L7UQJ2_HUMAN | Autophagy protein 5 | ATG5 | 162 | Annotation score: | ||
Q7Z3H3 | Q7Z3H3_HUMAN | Autophagy protein 5 | ATG5 DKFZp686F0183 | 91 | Annotation score: | ||
L7UMD8 | L7UMD8_HUMAN | ATG5 variant 3 | ATG5 | 44 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036243 | 58 | K → M in a colorectal cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_079274 | 122 | E → D in SCAR25; reduced conjugation to ATG12; decrease in autophagy activity. 1 PublicationCorresponds to variant dbSNP:rs1131692265EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003791 | 1 – 79 | MTDDK…TPLKW → M in isoform Short. 2 PublicationsAdd BLAST | 79 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y11588 mRNA Translation: CAA72327.1 AF293841 mRNA Translation: AAG44955.1 CR749386 mRNA Translation: CAH18236.1 AL022067 Genomic DNA No translation available. AL133509 Genomic DNA No translation available. AL138917 Genomic DNA No translation available. BC002699 mRNA Translation: AAH02699.1 BC093011 mRNA Translation: AAH93011.1 |
CCDSi | CCDS5055.1 [Q9H1Y0-1] CCDS69159.1 [Q9H1Y0-2] |
RefSeqi | NP_001273035.1, NM_001286106.1 [Q9H1Y0-1] NP_001273036.1, NM_001286107.1 [Q9H1Y0-2] NP_001273037.1, NM_001286108.1 NP_001273040.1, NM_001286111.1 NP_004840.1, NM_004849.3 [Q9H1Y0-1] |
Genome annotation databases
Ensembli | ENST00000343245; ENSP00000343313; ENSG00000057663 [Q9H1Y0-1] ENST00000369076; ENSP00000358072; ENSG00000057663 [Q9H1Y0-1] ENST00000635758; ENSP00000490493; ENSG00000057663 [Q9H1Y0-2] |
GeneIDi | 9474 |
KEGGi | hsa:9474 |
UCSCi | uc003prf.4, human [Q9H1Y0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y11588 mRNA Translation: CAA72327.1 AF293841 mRNA Translation: AAG44955.1 CR749386 mRNA Translation: CAH18236.1 AL022067 Genomic DNA No translation available. AL133509 Genomic DNA No translation available. AL138917 Genomic DNA No translation available. BC002699 mRNA Translation: AAH02699.1 BC093011 mRNA Translation: AAH93011.1 |
CCDSi | CCDS5055.1 [Q9H1Y0-1] CCDS69159.1 [Q9H1Y0-2] |
RefSeqi | NP_001273035.1, NM_001286106.1 [Q9H1Y0-1] NP_001273036.1, NM_001286107.1 [Q9H1Y0-2] NP_001273037.1, NM_001286108.1 NP_001273040.1, NM_001286111.1 NP_004840.1, NM_004849.3 [Q9H1Y0-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4GDK | X-ray | 2.70 | B/E | 1-275 | [»] | |
4GDL | X-ray | 2.88 | B | 1-275 | [»] | |
4NAW | X-ray | 2.20 | B/F/J/N | 1-275 | [»] | |
4TQ0 | X-ray | 2.70 | A/C/E | 1-275 | [»] | |
4TQ1 | X-ray | 1.80 | A | 1-275 | [»] | |
5D7G | X-ray | 3.00 | A/C/E/G | 1-275 | [»] | |
5NPV | X-ray | 3.10 | A/C | 1-275 | [»] | |
5NPW | X-ray | 3.10 | A/C/E/G | 1-275 | [»] | |
SMRi | Q9H1Y0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114859, 66 interactors |
ComplexPortali | CPX-200, ATG12-ATG5-ATG16L1 complex CPX-354, ATG12-ATG5-ATG16L2 complex CPX-356, ATG5-ATG12 complex CPX-358, ATG5-ATG12-TECPR1 complex |
DIPi | DIP-29758N |
IntActi | Q9H1Y0, 55 interactors |
MINTi | Q9H1Y0 |
STRINGi | 9606.ENSP00000358072 |
PTM databases
iPTMneti | Q9H1Y0 |
PhosphoSitePlusi | Q9H1Y0 |
Genetic variation databases
BioMutai | ATG5 |
DMDMi | 17366828 |
Proteomic databases
EPDi | Q9H1Y0 |
jPOSTi | Q9H1Y0 |
MassIVEi | Q9H1Y0 |
MaxQBi | Q9H1Y0 |
PaxDbi | Q9H1Y0 |
PeptideAtlasi | Q9H1Y0 |
PRIDEi | Q9H1Y0 |
ProteomicsDBi | 80456 [Q9H1Y0-1] 80457 [Q9H1Y0-2] |
Protocols and materials databases
Antibodypediai | 32129, 1033 antibodies |
DNASUi | 9474 |
Genome annotation databases
Ensembli | ENST00000343245; ENSP00000343313; ENSG00000057663 [Q9H1Y0-1] ENST00000369076; ENSP00000358072; ENSG00000057663 [Q9H1Y0-1] ENST00000635758; ENSP00000490493; ENSG00000057663 [Q9H1Y0-2] |
GeneIDi | 9474 |
KEGGi | hsa:9474 |
UCSCi | uc003prf.4, human [Q9H1Y0-1] |
Organism-specific databases
CTDi | 9474 |
DisGeNETi | 9474 |
GeneCardsi | ATG5 |
HGNCi | HGNC:589, ATG5 |
HPAi | ENSG00000057663, Low tissue specificity |
MalaCardsi | ATG5 |
MIMi | 604261, gene 617584, phenotype |
neXtProti | NX_Q9H1Y0 |
OpenTargetsi | ENSG00000057663 |
PharmGKBi | PA24880 |
VEuPathDBi | HostDB:ENSG00000057663.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2976, Eukaryota |
GeneTreei | ENSGT00390000004766 |
HOGENOMi | CLU_051894_1_0_1 |
InParanoidi | Q9H1Y0 |
OMAi | MVPRLSY |
PhylomeDBi | Q9H1Y0 |
TreeFami | TF314415 |
Enzyme and pathway databases
PathwayCommonsi | Q9H1Y0 |
Reactomei | R-HSA-1632852, Macroautophagy R-HSA-5205685, PINK1-PRKN Mediated Mitophagy R-HSA-8934903, Receptor Mediated Mitophagy R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling |
SIGNORi | Q9H1Y0 |
Miscellaneous databases
BioGRID-ORCSi | 9474, 16 hits in 1004 CRISPR screens |
ChiTaRSi | ATG5, human |
GeneWikii | ATG5 |
GenomeRNAii | 9474 |
Pharosi | Q9H1Y0, Tbio |
PROi | PR:Q9H1Y0 |
RNActi | Q9H1Y0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000057663, Expressed in colonic mucosa and 236 other tissues |
ExpressionAtlasi | Q9H1Y0, baseline and differential |
Genevisiblei | Q9H1Y0, HS |
Family and domain databases
Gene3Di | 1.10.246.190, 1 hit 3.10.20.620, 1 hit |
InterProi | View protein in InterPro IPR007239, Atg5 IPR042526, Atg5_HR IPR042527, Atg5_UblA_dom |
PANTHERi | PTHR13040, PTHR13040, 1 hit |
Pfami | View protein in Pfam PF04106, APG5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG5_HUMAN | |
Accessioni | Q9H1Y0Primary (citable) accession number: Q9H1Y0 Secondary accession number(s): O60875 Q9HCZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | November 16, 2001 | |
Last modified: | April 7, 2021 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families