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Protein

Protein Wnt-8a

Gene

WNT8A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.By similarity

GO - Molecular functioni

  • frizzled binding Source: GO_Central
  • receptor ligand activity Source: WormBase

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-3238698 WNT ligand biogenesis and trafficking
R-HSA-373080 Class B/2 (Secretin family receptors)
R-HSA-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
SignaLinkiQ9H1J5
SIGNORiQ9H1J5

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8a
Alternative name(s):
Protein Wnt-8d
Gene namesi
Name:WNT8A
Synonyms:WNT8D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000061492.11
HGNCiHGNC:12788 WNT8A
MIMi606360 gene
neXtProtiNX_Q9H1J5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi7478
OpenTargetsiENSG00000061492
PharmGKBiPA37389

Polymorphism and mutation databases

BioMutaiWNT8A
DMDMi288558833

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000004144825 – 351Protein Wnt-8aAdd BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 65By similarity
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi104 ↔ 112By similarity
Disulfide bondi114 ↔ 132By similarity
Disulfide bondi180 ↔ 194By similarity
Disulfide bondi182 ↔ 189By similarity
Lipidationi186O-palmitoleoyl serineBy similarity1
Disulfide bondi259 ↔ 297By similarity
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi275 ↔ 290By similarity
Glycosylationi281N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi294 ↔ 336By similarity
Disulfide bondi312 ↔ 327By similarity
Disulfide bondi314 ↔ 324By similarity
Disulfide bondi319 ↔ 320By similarity

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors (By similarity). Depalmitoleoylation leads to Wnt signaling pathway inhibition (By similarity).By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT8A.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiQ9H1J5
PeptideAtlasiQ9H1J5
PRIDEiQ9H1J5
ProteomicsDBi80418
80419 [Q9H1J5-2]

2D gel databases

REPRODUCTION-2DPAGEiQ9H1J5

PTM databases

iPTMnetiQ9H1J5
PhosphoSitePlusiQ9H1J5

Expressioni

Gene expression databases

BgeeiENSG00000061492 Expressed in 17 organ(s), highest expression level in caudate nucleus
CleanExiHS_WNT8A
ExpressionAtlasiQ9H1J5 baseline and differential
GenevisibleiQ9H1J5 HS

Organism-specific databases

HPAiHPA038539

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720).

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113315, 1 interactor
STRINGi9606.ENSP00000381739

Structurei

3D structure databases

ProteinModelPortaliQ9H1J5
SMRiQ9H1J5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00690000101857
HOGENOMiHOG000039529
HOVERGENiHBG001595
InParanoidiQ9H1J5
KOiK00714
PhylomeDBiQ9H1J5
TreeFamiTF105310

Family and domain databases

InterProiView protein in InterPro
IPR034312 Protein_Wnt-8A/8C
IPR005817 Wnt
IPR013301 Wnt8
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PTHR12027:SF92 PTHR12027:SF92, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01892 WNT8PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H1J5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGNLFMLWAA LGICCAAFSA SAWSVNNFLI TGPKAYLTYT TSVALGAQSG
60 70 80 90 100
IEECKFQFAW ERWNCPENAL QLSTHNRLRS ATRETSFIHA ISSAGVMYII
110 120 130 140 150
TKNCSMGDFE NCGCDGSNNG KTGGHGWIWG GCSDNVEFGE RISKLFVDSL
160 170 180 190 200
EKGKDARALM NLHNNRAGRL AVRATMKRTC KCHGISGSCS IQTCWLQLAE
210 220 230 240 250
FREMGDYLKA KYDQALKIEM DKRQLRAGNS AEGHWVPAEA FLPSAEAELI
260 270 280 290 300
FLEESPDYCT CNSSLGIYGT EGRECLQNSH NTSRWERRSC GRLCTECGLQ
310 320 330 340 350
VEERKTEVIS SCNCKFQWCC TVKCDQCRHV VSKYYCARSP GSAQSLGKGS

A
Length:351
Mass (Da):38,849
Last modified:February 9, 2010 - v2
Checksum:iB3432171301C17E5
GO
Isoform 2 (identifier: Q9H1J5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-351: KGSA → RVWFGVYI

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:355
Mass (Da):39,527
Checksum:i605B1D5DD755FBD6
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RF47D6RF47_HUMAN
Protein Wnt
WNT8A
369Annotation score:
D6RF94D6RF94_HUMAN
Protein Wnt
WNT8A
360Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038706348 – 351KGSA → RVWFGVYI in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057725 mRNA Translation: BAB60960.1
AY009402 mRNA Translation: AAG38662.1
CCDSiCCDS43368.1 [Q9H1J5-1]
RefSeqiNP_001287867.1, NM_001300938.1
NP_001287868.1, NM_001300939.1
NP_490645.1, NM_058244.3 [Q9H1J5-1]
UniGeneiHs.591274

Genome annotation databases

EnsembliENST00000361560; ENSP00000354726; ENSG00000061492 [Q9H1J5-2]
ENST00000398754; ENSP00000381739; ENSG00000061492 [Q9H1J5-1]
GeneIDi7478
KEGGihsa:7478
UCSCiuc003lcd.1 human [Q9H1J5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057725 mRNA Translation: BAB60960.1
AY009402 mRNA Translation: AAG38662.1
CCDSiCCDS43368.1 [Q9H1J5-1]
RefSeqiNP_001287867.1, NM_001300938.1
NP_001287868.1, NM_001300939.1
NP_490645.1, NM_058244.3 [Q9H1J5-1]
UniGeneiHs.591274

3D structure databases

ProteinModelPortaliQ9H1J5
SMRiQ9H1J5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113315, 1 interactor
STRINGi9606.ENSP00000381739

PTM databases

iPTMnetiQ9H1J5
PhosphoSitePlusiQ9H1J5

Polymorphism and mutation databases

BioMutaiWNT8A
DMDMi288558833

2D gel databases

REPRODUCTION-2DPAGEiQ9H1J5

Proteomic databases

PaxDbiQ9H1J5
PeptideAtlasiQ9H1J5
PRIDEiQ9H1J5
ProteomicsDBi80418
80419 [Q9H1J5-2]

Protocols and materials databases

DNASUi7478
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361560; ENSP00000354726; ENSG00000061492 [Q9H1J5-2]
ENST00000398754; ENSP00000381739; ENSG00000061492 [Q9H1J5-1]
GeneIDi7478
KEGGihsa:7478
UCSCiuc003lcd.1 human [Q9H1J5-1]

Organism-specific databases

CTDi7478
DisGeNETi7478
EuPathDBiHostDB:ENSG00000061492.11
GeneCardsiWNT8A
HGNCiHGNC:12788 WNT8A
HPAiHPA038539
MIMi606360 gene
neXtProtiNX_Q9H1J5
OpenTargetsiENSG00000061492
PharmGKBiPA37389
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00690000101857
HOGENOMiHOG000039529
HOVERGENiHBG001595
InParanoidiQ9H1J5
KOiK00714
PhylomeDBiQ9H1J5
TreeFamiTF105310

Enzyme and pathway databases

ReactomeiR-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-3238698 WNT ligand biogenesis and trafficking
R-HSA-373080 Class B/2 (Secretin family receptors)
R-HSA-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
SignaLinkiQ9H1J5
SIGNORiQ9H1J5

Miscellaneous databases

GenomeRNAii7478
PROiPR:Q9H1J5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000061492 Expressed in 17 organ(s), highest expression level in caudate nucleus
CleanExiHS_WNT8A
ExpressionAtlasiQ9H1J5 baseline and differential
GenevisibleiQ9H1J5 HS

Family and domain databases

InterProiView protein in InterPro
IPR034312 Protein_Wnt-8A/8C
IPR005817 Wnt
IPR013301 Wnt8
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PTHR12027:SF92 PTHR12027:SF92, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01892 WNT8PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWNT8A_HUMAN
AccessioniPrimary (citable) accession number: Q9H1J5
Secondary accession number(s): Q96S51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: February 9, 2010
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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