UniProtKB - Q9H1D9 (RPC6_HUMAN)
Protein
DNA-directed RNA polymerase III subunit RPC6
Gene
POLR3F
Organism
Homo sapiens (Human)
Status
Functioni
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628).3 Publications
GO - Molecular functioni
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB
- double-stranded DNA binding Source: UniProtKB
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- positive regulation of innate immune response Source: UniProtKB
- positive regulation of interferon-beta production Source: UniProtKB
- positive regulation of type I interferon production Source: Reactome
- regulation of transcription by RNA polymerase III Source: UniProtKB
- transcription by RNA polymerase III Source: InterPro
Keywordsi
Molecular function | DNA-binding |
Biological process | Antiviral defense, Immunity, Innate immunity, Transcription |
Enzyme and pathway databases
PathwayCommonsi | Q9H1D9 |
Reactomei | R-HSA-1834949, Cytosolic sensors of pathogen-associated DNA R-HSA-73780, RNA Polymerase III Chain Elongation R-HSA-73980, RNA Polymerase III Transcription Termination R-HSA-749476, RNA Polymerase III Abortive And Retractive Initiation R-HSA-76061, RNA Polymerase III Transcription Initiation From Type 1 Promoter R-HSA-76066, RNA Polymerase III Transcription Initiation From Type 2 Promoter R-HSA-76071, RNA Polymerase III Transcription Initiation From Type 3 Promoter |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-directed RNA polymerase III subunit RPC6Short name: RNA polymerase III subunit C6 Alternative name(s): DNA-directed RNA polymerase III subunit F RNA polymerase III 39 kDa subunit Short name: RPC39 |
Gene namesi | Name:POLR3F |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000132664.11 |
HGNCi | HGNC:15763, POLR3F |
MIMi | 617455, gene |
neXtProti | NX_Q9H1D9 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
- RNA polymerase III complex Source: MGI
Keywords - Cellular componenti
DNA-directed RNA polymerase, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 137 – 140 | KAVK → EAVE: Strongly impaired dsDNA-binding. No effect on interaction with POLR3C. 1 Publication | 4 | |
Mutagenesisi | 173 – 175 | ESE → KSK: Strongly impaired dsDNA-binding. No effect on interaction with POLR3C. 1 Publication | 3 |
Organism-specific databases
OpenTargetsi | ENSG00000132664 |
PharmGKBi | PA33520 |
Miscellaneous databases
Pharosi | Q9H1D9, Tbio |
Polymorphism and mutation databases
BioMutai | POLR3F |
DMDMi | 20139728 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000073972 | 2 – 316 | DNA-directed RNA polymerase III subunit RPC6Add BLAST | 315 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Cross-linki | 5 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 7 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q9H1D9 |
jPOSTi | Q9H1D9 |
MassIVEi | Q9H1D9 |
MaxQBi | Q9H1D9 |
PaxDbi | Q9H1D9 |
PeptideAtlasi | Q9H1D9 |
PRIDEi | Q9H1D9 |
ProteomicsDBi | 80402 |
PTM databases
iPTMneti | Q9H1D9 |
MetOSitei | Q9H1D9 |
PhosphoSitePlusi | Q9H1D9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000132664, Expressed in cerebellar hemisphere and 189 other tissues |
Genevisiblei | Q9H1D9, HS |
Organism-specific databases
HPAi | ENSG00000132664, Low tissue specificity |
Interactioni
Subunit structurei
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex (By similarity). Directly interacts with POLR3C (PubMed:21358628).
Interacts with TBP and TFIIIB90 and GTF3C4 (By similarity) (PubMed:10523658).
Interacts with MAF1 (PubMed:18377933).
By similarity4 PublicationsBinary interactionsi
Hide detailsQ9H1D9
Protein-protein interaction databases
BioGRIDi | 115866, 42 interactors |
DIPi | DIP-34646N |
IntActi | Q9H1D9, 38 interactors |
MINTi | Q9H1D9 |
STRINGi | 9606.ENSP00000366828 |
Miscellaneous databases
RNActi | Q9H1D9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H1D9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9H1D9 |
Family & Domainsi
Sequence similaritiesi
Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.Curated
Phylogenomic databases
eggNOGi | KOG3233, Eukaryota |
GeneTreei | ENSGT00390000009679 |
HOGENOMi | CLU_033661_1_0_1 |
InParanoidi | Q9H1D9 |
OMAi | CEYMRDW |
OrthoDBi | 1287786at2759 |
PhylomeDBi | Q9H1D9 |
TreeFami | TF103051 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR007832, RNA_pol_Rpc34 IPR016049, RNA_pol_Rpc34-like IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12780, PTHR12780, 1 hit |
Pfami | View protein in Pfam PF05158, RNA_pol_Rpc34, 1 hit |
PIRSFi | PIRSF028763, RNA_pol_Rpc34, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9H1D9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR
60 70 80 90 100
AVAINRLLSM GQLDLLRSNT GLLYRIKDSQ NAGKMKGSDN QEKLVYQIIE
110 120 130 140 150
DAGNKGIWSR DIRYKSNLPL TEINKILKNL ESKKLIKAVK SVAASKKKVY
160 170 180 190 200
MLYNLQPDRS VTGGAWYSDQ DFESEFVEVL NQQCFKFLQS KAETARESKQ
210 220 230 240 250
NPMIQRNSSF ASSHEVWKYI CELGISKVEL SMEDIETILN TLIYDGKVEM
260 270 280 290 300
TIIAAKEGTV GSVDGHMKLY RAVNPIIPPT GLVRAPCGLC PVFDDCHEGG
310
EISPSNCIYM TEWLEF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | A → G in AAB63677 (PubMed:9171375).Curated | 1 | |
Sequence conflicti | 44 – 53 | HIEAQQRAVA → QYRSPAAGSS in AAB63677 (PubMed:9171375).Curated | 10 | |
Sequence conflicti | 255 – 290 | AKEGT…PCGLC → CKRRHSWQCRWTHETVQGSQ SNHPSHRFGPGHPVDSA in AAB63677 (PubMed:9171375).CuratedAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93869 mRNA Translation: AAB63677.1 AK290892 mRNA Translation: BAF83581.1 AL121893 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10240.1 BC012588 mRNA Translation: AAH12588.1 |
CCDSi | CCDS13135.1 |
RefSeqi | NP_001269455.1, NM_001282526.1 NP_006457.2, NM_006466.3 |
Genome annotation databases
Ensembli | ENST00000377603; ENSP00000366828; ENSG00000132664 |
GeneIDi | 10621 |
KEGGi | hsa:10621 |
UCSCi | uc002wqv.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93869 mRNA Translation: AAB63677.1 AK290892 mRNA Translation: BAF83581.1 AL121893 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10240.1 BC012588 mRNA Translation: AAH12588.1 |
CCDSi | CCDS13135.1 |
RefSeqi | NP_001269455.1, NM_001282526.1 NP_006457.2, NM_006466.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DK5 | NMR | - | A | 78-155 | [»] | |
2YU3 | NMR | - | A | 61-142 | [»] | |
SMRi | Q9H1D9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115866, 42 interactors |
DIPi | DIP-34646N |
IntActi | Q9H1D9, 38 interactors |
MINTi | Q9H1D9 |
STRINGi | 9606.ENSP00000366828 |
PTM databases
iPTMneti | Q9H1D9 |
MetOSitei | Q9H1D9 |
PhosphoSitePlusi | Q9H1D9 |
Polymorphism and mutation databases
BioMutai | POLR3F |
DMDMi | 20139728 |
Proteomic databases
EPDi | Q9H1D9 |
jPOSTi | Q9H1D9 |
MassIVEi | Q9H1D9 |
MaxQBi | Q9H1D9 |
PaxDbi | Q9H1D9 |
PeptideAtlasi | Q9H1D9 |
PRIDEi | Q9H1D9 |
ProteomicsDBi | 80402 |
Protocols and materials databases
Antibodypediai | 24572, 188 antibodies |
DNASUi | 10621 |
Genome annotation databases
Ensembli | ENST00000377603; ENSP00000366828; ENSG00000132664 |
GeneIDi | 10621 |
KEGGi | hsa:10621 |
UCSCi | uc002wqv.5, human |
Organism-specific databases
CTDi | 10621 |
EuPathDBi | HostDB:ENSG00000132664.11 |
GeneCardsi | POLR3F |
HGNCi | HGNC:15763, POLR3F |
HPAi | ENSG00000132664, Low tissue specificity |
MIMi | 617455, gene |
neXtProti | NX_Q9H1D9 |
OpenTargetsi | ENSG00000132664 |
PharmGKBi | PA33520 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3233, Eukaryota |
GeneTreei | ENSGT00390000009679 |
HOGENOMi | CLU_033661_1_0_1 |
InParanoidi | Q9H1D9 |
OMAi | CEYMRDW |
OrthoDBi | 1287786at2759 |
PhylomeDBi | Q9H1D9 |
TreeFami | TF103051 |
Enzyme and pathway databases
PathwayCommonsi | Q9H1D9 |
Reactomei | R-HSA-1834949, Cytosolic sensors of pathogen-associated DNA R-HSA-73780, RNA Polymerase III Chain Elongation R-HSA-73980, RNA Polymerase III Transcription Termination R-HSA-749476, RNA Polymerase III Abortive And Retractive Initiation R-HSA-76061, RNA Polymerase III Transcription Initiation From Type 1 Promoter R-HSA-76066, RNA Polymerase III Transcription Initiation From Type 2 Promoter R-HSA-76071, RNA Polymerase III Transcription Initiation From Type 3 Promoter |
Miscellaneous databases
BioGRID-ORCSi | 10621, 688 hits in 859 CRISPR screens |
ChiTaRSi | POLR3F, human |
EvolutionaryTracei | Q9H1D9 |
GeneWikii | POLR3F |
GenomeRNAii | 10621 |
Pharosi | Q9H1D9, Tbio |
PROi | PR:Q9H1D9 |
RNActi | Q9H1D9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132664, Expressed in cerebellar hemisphere and 189 other tissues |
Genevisiblei | Q9H1D9, HS |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR007832, RNA_pol_Rpc34 IPR016049, RNA_pol_Rpc34-like IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12780, PTHR12780, 1 hit |
Pfami | View protein in Pfam PF05158, RNA_pol_Rpc34, 1 hit |
PIRSFi | PIRSF028763, RNA_pol_Rpc34, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPC6_HUMAN | |
Accessioni | Q9H1D9Primary (citable) accession number: Q9H1D9 Secondary accession number(s): A8K4C7, O15319 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM