UniProtKB - Q9H0X9 (OSBL5_HUMAN)
Protein
Oxysterol-binding protein-related protein 5
Gene
OSBPL5
Organism
Homo sapiens (Human)
Status
Functioni
Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:23934110, PubMed:26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed:21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).4 Publications
Miscellaneous
According to a report, the gene is imprinted in placenta, where it is predominantly expressed from the maternal allele only. Not imprinted in other tissues (PubMed:12504849). According to another report, it is not imprinted in trophoblast stem cells (PubMed:20644730).2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 449 | PhosphatidylserineBy similarity | 1 | |
Binding sitei | 504 | PhosphatidylserineBy similarity | 1 | |
Binding sitei | 670 | Phosphatidylinositol 4-phosphateBy similarity | 1 | |
Binding sitei | 674 | Phosphatidylinositol 4-phosphateBy similarity | 1 | |
Binding sitei | 678 | Phosphatidylinositol 4-phosphateBy similarity | 1 |
GO - Molecular functioni
- cholesterol binding Source: BHF-UCL
- lipid binding Source: GO_Central
- oxysterol binding Source: UniProtKB
- phosphatidylinositol-4-phosphate binding Source: UniProtKB
- phosphatidylserine binding Source: UniProtKB
- phosphatidylserine transfer activity Source: GO_Central
- phospholipid transporter activity Source: Reactome
- sterol binding Source: GO_Central
- sterol transporter activity Source: GO_Central
GO - Biological processi
- cholesterol metabolic process Source: UniProtKB
- cholesterol transport Source: UniProtKB
- Golgi to plasma membrane transport Source: UniProtKB
- phosphatidylserine acyl-chain remodeling Source: Reactome
- phospholipid transport Source: UniProtKB
Keywordsi
Biological process | Lipid transport, Transport |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H0X9 |
Reactomei | R-HSA-1482801, Acyl chain remodelling of PS |
Protein family/group databases
TCDBi | 2.D.1.1.1, the pi4p/ps counter transporter (p/p-ct) family |
Chemistry databases
SwissLipidsi | SLP:000000527 |
Names & Taxonomyi
Protein namesi | Recommended name: Oxysterol-binding protein-related protein 5Short name: ORP-5 Short name: OSBP-related protein 5 Alternative name(s): Oxysterol-binding protein homolog 11 Publication |
Gene namesi | Name:OSBPL5 Synonyms:KIAA1534, OBPH11 Publication, ORP5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000021762.19 |
HGNCi | HGNC:16392, OSBPL5 |
MIMi | 606733, gene |
neXtProti | NX_Q9H0X9 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Single-pass membrane protein 1 Publication
Note: Localizes to endoplasmic reticulum-plasma membrane contact sites (EPCS). Localizes to the cortical endoplasmic reticulum at the EPCS.2 Publications
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- endoplasmic reticulum-plasma membrane contact site Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: HPA
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 860 – 878 | HelicalSequence analysisAdd BLAST | 19 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 389 | L → D: Impaired lipid countertransport between the endoplasmic reticulum and the plasma membrane. 1 Publication | 1 | |
Mutagenesisi | 478 – 479 | HH → AA: Impaired lipid countertransport between the endoplasmic reticulum and the plasma membrane. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 114879 |
OpenTargetsi | ENSG00000021762 |
PharmGKBi | PA32829 |
Miscellaneous databases
Pharosi | Q9H0X9, Tbio |
Polymorphism and mutation databases
BioMutai | OSBPL5 |
DMDMi | 20139173 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100373 | 1 – 879 | Oxysterol-binding protein-related protein 5Add BLAST | 879 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphoserineBy similarity | 1 | |
Modified residuei | 747 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9H0X9 |
jPOSTi | Q9H0X9 |
MassIVEi | Q9H0X9 |
MaxQBi | Q9H0X9 |
PaxDbi | Q9H0X9 |
PeptideAtlasi | Q9H0X9 |
PRIDEi | Q9H0X9 |
ProteomicsDBi | 5257 80342 [Q9H0X9-1] 80343 [Q9H0X9-2] |
PTM databases
iPTMneti | Q9H0X9 |
PhosphoSitePlusi | Q9H0X9 |
Expressioni
Tissue specificityi
Ubiquitously expressed.2 Publications
Gene expression databases
Bgeei | ENSG00000021762, Expressed in endocervix and 212 other tissues |
ExpressionAtlasi | Q9H0X9, baseline and differential |
Genevisiblei | Q9H0X9, HS |
Organism-specific databases
HPAi | ENSG00000021762, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 125380, 24 interactors |
DIPi | DIP-52332N |
IntActi | Q9H0X9, 22 interactors |
STRINGi | 9606.ENSP00000263650 |
Miscellaneous databases
RNActi | Q9H0X9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 126 – 243 | PHPROSITE-ProRule annotationAdd BLAST | 118 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 384 – 389 | Phosphatidylinositol 4-phosphate bindingBy similarity | 6 | |
Regioni | 384 – 389 | Phosphatidylserine bindingBy similarity | 6 | |
Regioni | 446 – 449 | Phosphatidylinositol 4-phosphate bindingBy similarity | 4 | |
Regioni | 478 – 479 | Phosphatidylinositol 4-phosphate bindingBy similarity | 2 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 93 – 123 | Sequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Belongs to the OSBP family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2210, Eukaryota |
GeneTreei | ENSGT00940000159535 |
HOGENOMi | CLU_012334_2_1_1 |
InParanoidi | Q9H0X9 |
OMAi | WDNEVFI |
OrthoDBi | 949920at2759 |
PhylomeDBi | Q9H0X9 |
TreeFami | TF312807 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR037239, OSBP_sf IPR000648, Oxysterol-bd IPR018494, Oxysterol-bd_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR10972, PTHR10972, 1 hit |
Pfami | View protein in Pfam PF01237, Oxysterol_BP, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
SUPFAMi | SSF144000, SSF144000, 1 hit |
PROSITEi | View protein in PROSITE PS01013, OSBP, 1 hit PS50003, PH_DOMAIN, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9H0X9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKEEAFLRRR FSLCPPSSTP QKVDPRKLTR NLLLSGDNEL YPLSPGKDME
60 70 80 90 100
PNGPSLPRDE GPPTPSSATK VPPAEYRLCN GSDKECVSPT ARVTKKETLK
110 120 130 140 150
AQKENYRQEK KRATRQLLSA LTDPSVVIMA DSLKIRGTLK SWTKLWCVLK
160 170 180 190 200
PGVLLIYKTP KVGQWVGTVL LHCCELIERP SKKDGFCFKL FHPLDQSVWA
210 220 230 240 250
VKGPKGESVG SITQPLPSSY LIFRAASESD GRCWLDALEL ALRCSSLLRL
260 270 280 290 300
GTCKPGRDGE PGTSPDASPS SLCGLPASAT VHPDQDLFPL NGSSLENDAF
310 320 330 340 350
SDKSERENPE ESDTETQDHS RKTESGSDQS ETPGAPVRRG TTYVEQVQEE
360 370 380 390 400
LGELGEASQV ETVSEENKSL MWTLLKQLRP GMDLSRVVLP TFVLEPRSFL
410 420 430 440 450
NKLSDYYYHA DLLSRAAVEE DAYSRMKLVL RWYLSGFYKK PKGIKKPYNP
460 470 480 490 500
ILGETFRCCW FHPQTDSRTF YIAEQVSHHP PVSAFHVSNR KDGFCISGSI
510 520 530 540 550
TAKSRFYGNS LSALLDGKAT LTFLNRAEDY TLTMPYAHCK GILYGTMTLE
560 570 580 590 600
LGGKVTIECA KNNFQAQLEF KLKPFFGGST SINQISGKIT SGEEVLASLS
610 620 630 640 650
GHWDRDVFIK EEGSGSSALF WTPSGEVRRQ RLRQHTVPLE EQTELESERL
660 670 680 690 700
WQHVTRAISK GDQHRATQEK FALEEAQRQR ARERQESLMP WKPQLFHLDP
710 720 730 740 750
ITQEWHYRYE DHSPWDPLKD IAQFEQDGIL RTLQQEAVAR QTTFLGSPGP
760 770 780 790 800
RHERSGPDQR LRKASDQPSG HSQATESSGS TPESCPELSD EEQDGDFVPG
810 820 830 840 850
GESPCPRCRK EARRLQALHE AILSIREAQQ ELHRHLSAML SSTARAAQAP
860 870
TPGLLQSPRS WFLLCVFLAC QLFINHILK
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YCD7 | H0YCD7_HUMAN | Oxysterol-binding protein | OSBPL5 | 432 | Annotation score: | ||
E9PPQ2 | E9PPQ2_HUMAN | Oxysterol-binding protein-related p... | OSBPL5 | 139 | Annotation score: | ||
E9PJE6 | E9PJE6_HUMAN | Oxysterol-binding protein-related p... | OSBPL5 | 124 | Annotation score: | ||
E9PNH0 | E9PNH0_HUMAN | Oxysterol-binding protein-related p... | OSBPL5 | 333 | Annotation score: | ||
E9PIJ6 | E9PIJ6_HUMAN | Oxysterol-binding protein-related p... | OSBPL5 | 98 | Annotation score: | ||
E9PQB4 | E9PQB4_HUMAN | Oxysterol-binding protein-related p... | OSBPL5 | 89 | Annotation score: | ||
E9PRA9 | E9PRA9_HUMAN | Oxysterol-binding protein-related p... | OSBPL5 | 115 | Annotation score: | ||
E9PLN3 | E9PLN3_HUMAN | Oxysterol-binding protein-related p... | OSBPL5 | 69 | Annotation score: |
Sequence cautioni
The sequence BAA96058 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_060079 | 90 | T → I. Corresponds to variant dbSNP:rs6578323Ensembl. | 1 | |
Natural variantiVAR_020414 | 774 | A → T. Corresponds to variant dbSNP:rs2277301Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057408 | 1 – 48 | Missing in isoform 3. 1 PublicationAdd BLAST | 48 | |
Alternative sequenceiVSP_043071 | 134 – 201 | Missing in isoform 2. 1 PublicationAdd BLAST | 68 | |
Alternative sequenceiVSP_057409 | 161 – 201 | Missing in isoform 3. 1 PublicationAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB074006 mRNA Translation: BAB85686.1 AF392453 mRNA Translation: AAL40666.1 AF410430 mRNA Translation: AAK98617.1 AL136918 mRNA Translation: CAB66852.1 AK056510 mRNA Translation: BAG51735.1 AK301003 mRNA Translation: BAG62622.1 AC108448 Genomic DNA No translation available. KC877382 Genomic DNA No translation available. KF455307 Genomic DNA No translation available. KF455310 Genomic DNA No translation available. CH471158 Genomic DNA Translation: EAX02542.1 CH471158 Genomic DNA Translation: EAX02543.1 BC032646 mRNA Translation: AAH32646.2 BC039579 mRNA Translation: AAH39579.1 AF331964 mRNA Translation: AAG53417.1 AB040967 mRNA Translation: BAA96058.1 Sequence problems. |
CCDSi | CCDS31343.1 [Q9H0X9-2] CCDS31344.1 [Q9H0X9-1] |
RefSeqi | NP_001137535.1, NM_001144063.1 [Q9H0X9-2] NP_065947.1, NM_020896.3 [Q9H0X9-1] NP_663613.1, NM_145638.2 [Q9H0X9-2] XP_011518175.1, XM_011519873.2 [Q9H0X9-1] XP_016872652.1, XM_017017163.1 [Q9H0X9-1] |
Genome annotation databases
Ensembli | ENST00000263650; ENSP00000263650; ENSG00000021762 [Q9H0X9-1] ENST00000348039; ENSP00000302872; ENSG00000021762 [Q9H0X9-2] ENST00000389989; ENSP00000374639; ENSG00000021762 [Q9H0X9-2] ENST00000525498; ENSP00000433342; ENSG00000021762 [Q9H0X9-3] |
GeneIDi | 114879 |
KEGGi | hsa:114879 |
UCSCi | uc001lxk.3, human [Q9H0X9-1] uc010qxq.2, human |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB074006 mRNA Translation: BAB85686.1 AF392453 mRNA Translation: AAL40666.1 AF410430 mRNA Translation: AAK98617.1 AL136918 mRNA Translation: CAB66852.1 AK056510 mRNA Translation: BAG51735.1 AK301003 mRNA Translation: BAG62622.1 AC108448 Genomic DNA No translation available. KC877382 Genomic DNA No translation available. KF455307 Genomic DNA No translation available. KF455310 Genomic DNA No translation available. CH471158 Genomic DNA Translation: EAX02542.1 CH471158 Genomic DNA Translation: EAX02543.1 BC032646 mRNA Translation: AAH32646.2 BC039579 mRNA Translation: AAH39579.1 AF331964 mRNA Translation: AAG53417.1 AB040967 mRNA Translation: BAA96058.1 Sequence problems. |
CCDSi | CCDS31343.1 [Q9H0X9-2] CCDS31344.1 [Q9H0X9-1] |
RefSeqi | NP_001137535.1, NM_001144063.1 [Q9H0X9-2] NP_065947.1, NM_020896.3 [Q9H0X9-1] NP_663613.1, NM_145638.2 [Q9H0X9-2] XP_011518175.1, XM_011519873.2 [Q9H0X9-1] XP_016872652.1, XM_017017163.1 [Q9H0X9-1] |
3D structure databases
SMRi | Q9H0X9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125380, 24 interactors |
DIPi | DIP-52332N |
IntActi | Q9H0X9, 22 interactors |
STRINGi | 9606.ENSP00000263650 |
Chemistry databases
SwissLipidsi | SLP:000000527 |
Protein family/group databases
TCDBi | 2.D.1.1.1, the pi4p/ps counter transporter (p/p-ct) family |
PTM databases
iPTMneti | Q9H0X9 |
PhosphoSitePlusi | Q9H0X9 |
Polymorphism and mutation databases
BioMutai | OSBPL5 |
DMDMi | 20139173 |
Proteomic databases
EPDi | Q9H0X9 |
jPOSTi | Q9H0X9 |
MassIVEi | Q9H0X9 |
MaxQBi | Q9H0X9 |
PaxDbi | Q9H0X9 |
PeptideAtlasi | Q9H0X9 |
PRIDEi | Q9H0X9 |
ProteomicsDBi | 5257 80342 [Q9H0X9-1] 80343 [Q9H0X9-2] |
Protocols and materials databases
Antibodypediai | 23281, 78 antibodies |
DNASUi | 114879 |
Genome annotation databases
Ensembli | ENST00000263650; ENSP00000263650; ENSG00000021762 [Q9H0X9-1] ENST00000348039; ENSP00000302872; ENSG00000021762 [Q9H0X9-2] ENST00000389989; ENSP00000374639; ENSG00000021762 [Q9H0X9-2] ENST00000525498; ENSP00000433342; ENSG00000021762 [Q9H0X9-3] |
GeneIDi | 114879 |
KEGGi | hsa:114879 |
UCSCi | uc001lxk.3, human [Q9H0X9-1] uc010qxq.2, human |
Organism-specific databases
CTDi | 114879 |
DisGeNETi | 114879 |
EuPathDBi | HostDB:ENSG00000021762.19 |
GeneCardsi | OSBPL5 |
HGNCi | HGNC:16392, OSBPL5 |
HPAi | ENSG00000021762, Low tissue specificity |
MIMi | 606733, gene |
neXtProti | NX_Q9H0X9 |
OpenTargetsi | ENSG00000021762 |
PharmGKBi | PA32829 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2210, Eukaryota |
GeneTreei | ENSGT00940000159535 |
HOGENOMi | CLU_012334_2_1_1 |
InParanoidi | Q9H0X9 |
OMAi | WDNEVFI |
OrthoDBi | 949920at2759 |
PhylomeDBi | Q9H0X9 |
TreeFami | TF312807 |
Enzyme and pathway databases
PathwayCommonsi | Q9H0X9 |
Reactomei | R-HSA-1482801, Acyl chain remodelling of PS |
Miscellaneous databases
BioGRID-ORCSi | 114879, 5 hits in 839 CRISPR screens |
ChiTaRSi | OSBPL5, human |
GeneWikii | OSBPL5 |
GenomeRNAii | 114879 |
Pharosi | Q9H0X9, Tbio |
PROi | PR:Q9H0X9 |
RNActi | Q9H0X9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000021762, Expressed in endocervix and 212 other tissues |
ExpressionAtlasi | Q9H0X9, baseline and differential |
Genevisiblei | Q9H0X9, HS |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR037239, OSBP_sf IPR000648, Oxysterol-bd IPR018494, Oxysterol-bd_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR10972, PTHR10972, 1 hit |
Pfami | View protein in Pfam PF01237, Oxysterol_BP, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
SUPFAMi | SSF144000, SSF144000, 1 hit |
PROSITEi | View protein in PROSITE PS01013, OSBP, 1 hit PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OSBL5_HUMAN | |
Accessioni | Q9H0X9Primary (citable) accession number: Q9H0X9 Secondary accession number(s): A6NDP0 Q9P1Z4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 11, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations