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Protein

Probable ATP-dependent RNA helicase DDX47

Gene

DDX47

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in apoptosis. May have a role in rRNA processing and mRNA splicing. Associates with pre-rRNA precursors.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 75ATP8

GO - Molecular functioni

GO - Biological processi

  • extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • RNA secondary structure unwinding Source: GO_Central
  • RNA splicing Source: UniProtKB
  • rRNA processing Source: UniProtKB

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processApoptosis, mRNA processing, mRNA splicing, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6790901 rRNA modification in the nucleus and cytosol
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX47 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 47
Gene namesi
Name:DDX47
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000213782.7
HGNCiHGNC:18682 DDX47
MIMi615428 gene
neXtProtiNX_Q9H0S4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000213782
PharmGKBiPA134918403

Polymorphism and mutation databases

BioMutaiDDX47
DMDMi52782792

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000550502 – 455Probable ATP-dependent RNA helicase DDX47Add BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei9PhosphoserineCombined sources1
Modified residuei149PhosphothreonineCombined sources1
Modified residuei424PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H0S4
MaxQBiQ9H0S4
PaxDbiQ9H0S4
PeptideAtlasiQ9H0S4
PRIDEiQ9H0S4
ProteomicsDBi80322

2D gel databases

SWISS-2DPAGEiQ9H0S4

PTM databases

iPTMnetiQ9H0S4
PhosphoSitePlusiQ9H0S4
SwissPalmiQ9H0S4

Expressioni

Gene expression databases

BgeeiENSG00000213782
CleanExiHS_DDX47
ExpressionAtlasiQ9H0S4 baseline and differential
GenevisibleiQ9H0S4 HS

Organism-specific databases

HPAiHPA014855

Interactioni

Subunit structurei

Interacts with AGO1 and AGO2. Interacts with GABARAP. Interacts with NOL8; the interaction is RNA-dependent.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GABARAPO951663EBI-2515241,EBI-712001

Protein-protein interaction databases

BioGridi119375, 65 interactors
IntActiQ9H0S4, 36 interactors
MINTiQ9H0S4
STRINGi9606.ENSP00000350698

Structurei

Secondary structure

1455
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 23Combined sources6
Helixi27 – 29Combined sources3
Helixi33 – 41Combined sources9
Helixi49 – 59Combined sources11
Beta strandi64 – 67Combined sources4
Helixi74 – 88Combined sources15
Beta strandi95 – 98Combined sources4
Helixi102 – 116Combined sources15
Helixi117 – 119Combined sources3
Beta strandi123 – 126Combined sources4
Helixi132 – 140Combined sources9
Beta strandi144 – 148Combined sources5
Helixi150 – 159Combined sources10
Beta strandi170 – 173Combined sources4
Helixi176 – 181Combined sources6
Helixi185 – 193Combined sources9
Beta strandi197 – 207Combined sources11
Helixi210 – 219Combined sources10
Beta strandi224 – 227Combined sources4

3D structure databases

ProteinModelPortaliQ9H0S4
SMRiQ9H0S4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H0S4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 226Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini237 – 397Helicase C-terminalPROSITE-ProRule annotationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi24 – 52Q motifAdd BLAST29
Motifi174 – 177DEAD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0330 Eukaryota
ENOG410XQU7 LUCA
GeneTreeiENSGT00730000110914
HOGENOMiHOG000268802
HOVERGENiHBG100512
InParanoidiQ9H0S4
KOiK14777
OMAiLNMDFEV
OrthoDBiEOG091G07QH
PhylomeDBiQ9H0S4
TreeFamiTF105714

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H0S4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPEEHDSP TEASQPIVEE EETKTFKDLG VTDVLCEACD QLGWTKPTKI
60 70 80 90 100
QIEAIPLALQ GRDIIGLAET GSGKTGAFAL PILNALLETP QRLFALVLTP
110 120 130 140 150
TRELAFQISE QFEALGSSIG VQSAVIVGGI DSMSQSLALA KKPHIIIATP
160 170 180 190 200
GRLIDHLENT KGFNLRALKY LVMDEADRIL NMDFETEVDK ILKVIPRDRK
210 220 230 240 250
TFLFSATMTK KVQKLQRAAL KNPVKCAVSS KYQTVEKLQQ YYIFIPSKFK
260 270 280 290 300
DTYLVYILNE LAGNSFMIFC STCNNTQRTA LLLRNLGFTA IPLHGQMSQS
310 320 330 340 350
KRLGSLNKFK AKARSILLAT DVASRGLDIP HVDVVVNFDI PTHSKDYIHR
360 370 380 390 400
VGRTARAGRS GKAITFVTQY DVELFQRIEH LIGKKLPGFP TQDDEVMMLT
410 420 430 440 450
ERVAEAQRFA RMELREHGEK KKRSREDAGD NDDTEGAIGV RNKVAGGKMK

KRKGR
Length:455
Mass (Da):50,647
Last modified:March 1, 2001 - v1
Checksum:i5F64F26AD2C11286
GO
Isoform 2 (identifier: Q9H0S4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     251-299: Missing.

Note: No experimental confirmation available.
Show »
Length:406
Mass (Da):45,169
Checksum:iE6FFADA03AFAB679
GO

Sequence cautioni

The sequence AAF23354 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55I → V in BAB70762 (PubMed:16541075).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045239251 – 299Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136666 mRNA Translation: CAB66601.1
AK054574 mRNA Translation: BAB70762.1
AK127712 mRNA Translation: BAG54556.1
AC007215 Genomic DNA No translation available.
CH471094 Genomic DNA Translation: EAW96277.1
CH471094 Genomic DNA Translation: EAW96283.1
BC009379 mRNA Translation: AAH09379.2
BC068009 mRNA Translation: AAH68009.1
AF078843 mRNA Translation: AAF23354.1 Different initiation.
CCDSiCCDS8655.1 [Q9H0S4-1]
CCDS8656.1 [Q9H0S4-2]
RefSeqiNP_057439.2, NM_016355.3 [Q9H0S4-1]
NP_957518.1, NM_201224.1 [Q9H0S4-2]
UniGeneiHs.719938

Genome annotation databases

EnsembliENST00000352940; ENSP00000319578; ENSG00000213782 [Q9H0S4-2]
ENST00000358007; ENSP00000350698; ENSG00000213782 [Q9H0S4-1]
GeneIDi51202
KEGGihsa:51202
UCSCiuc001rax.4 human [Q9H0S4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDDX47_HUMAN
AccessioniPrimary (citable) accession number: Q9H0S4
Secondary accession number(s): B3KXP4
, G5E955, Q96GM0, Q96NV8, Q9UI98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2001
Last modified: June 20, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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