TKTL2

Functionⁱ

Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Plays a pivotal role in carcinogenesis.1 Publication

Catalytic activityⁱ

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.

Cofactorⁱ

Protein has several cofactor binding sites:

Mg²⁺By similarity, Ca²⁺By similarity, Mn²⁺By similarity, Co²⁺By similarityNote: Binds 1 Mg²⁺ ion per subunit. Can also utilize other divalent metal cations, such as Ca²⁺, Mn²⁺ and Co²⁺.By similarity
thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	41	Thiamine pyrophosphateBy similarity	1
Binding siteⁱ	78	Thiamine pyrophosphateBy similarity	1
Metal bindingⁱ	156	MagnesiumBy similarity	1
Binding siteⁱ	157	Thiamine pyrophosphate; via amide nitrogenBy similarity	1
Metal bindingⁱ	186	MagnesiumBy similarity	1
Binding siteⁱ	186	Thiamine pyrophosphateBy similarity	1
Metal bindingⁱ	188	Magnesium; via carbonyl oxygenBy similarity	1
Binding siteⁱ	248	Thiamine pyrophosphateBy similarity	1
Binding siteⁱ	262	SubstrateBy similarity	1
Binding siteⁱ	262	Thiamine pyrophosphateBy similarity	1
Siteⁱ	262	Important for catalytic activityBy similarity	1
Binding siteⁱ	349	SubstrateBy similarity	1
Active siteⁱ	370	Proton donorBy similarity	1
Binding siteⁱ	370	Thiamine pyrophosphateBy similarity	1
Binding siteⁱ	396	Thiamine pyrophosphateBy similarity	1
Binding siteⁱ	420	SubstrateBy similarity	1
Binding siteⁱ	428	SubstrateBy similarity	1
Binding siteⁱ	432	Thiamine pyrophosphateBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	124 – 126	Thiamine pyrophosphateBy similarity		3

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
transketolase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Ligand	Calcium, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BRENDAⁱ	2.2.1.1 2681

BRENDAⁱ

2.2.1.1 2681

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transketolase-like protein 2 (EC:2.2.1.1)
Gene namesⁱ	Name:TKTL2
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 4

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000151005.4
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:25313 TKTL2
neXtProtⁱ	NX_Q9H0I9

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	84076
Open Targets More...OpenTargetsⁱ	ENSG00000151005
PharmGKBⁱ	PA142670806

Polymorphism and mutation databases

BioMutaⁱ	TKTL2
DMDMⁱ	74717985

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000285200	1 – 626	Transketolase-like protein 2Add BLAST		626

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9H0I9
PaxDbⁱ	Q9H0I9
PeptideAtlas More...PeptideAtlasⁱ	Q9H0I9
PRIDEⁱ	Q9H0I9
ProteomicsDBⁱ	80285

PTM databases

iPTMnetⁱ	Q9H0I9
PhosphoSitePlusⁱ	Q9H0I9

Expressionⁱ

Tissue specificityⁱ

Overexpressed in hepatoma cancer cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000151005 Expressed in 15 organ(s), highest expression level in testis
CleanExⁱ	HS_TKTL2
ExpressionAtlasⁱ	Q9H0I9 baseline and differential
Genevisibleⁱ	Q9H0I9 HS

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Protein-protein interaction databases

BioGridⁱ	123871, 14 interactors
IntActⁱ	Q9H0I9, 1 interactor
STRINGⁱ	9606.ENSP00000280605

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9H0I9
SMRⁱ	Q9H0I9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the transketolase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0523 Eukaryota COG0021 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000005240
HOGENOMⁱ	HOG000243868
HOVERGENⁱ	HBG004036
InParanoidⁱ	Q9H0I9
KEGG Orthology (KO) More...KOⁱ	K00615
OMAⁱ	FADYMRG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03M5
PhylomeDBⁱ	Q9H0I9
TreeFamⁱ	TF313097

Family and domain databases

Gene3Dⁱ	3.40.50.920, 1 hit
InterProⁱ	View protein in InterPro IPR029061 THDP-binding IPR009014 Transketo_C/PFOR_II IPR005475 Transketolase-like_Pyr-bd IPR033248 Transketolase_C IPR005474 Transketolase_N
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02779 Transket_pyr, 1 hit PF02780 Transketolase_C, 1 hit PF00456 Transketolase_N, 1 hit
SMARTⁱ	View protein in SMART SM00861 Transket_pyr, 1 hit
SUPFAMⁱ	SSF52518 SSF52518, 2 hits SSF52922 SSF52922, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9H0I9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MMANDAKPDV KTVQVLRDTA NRLRIHSIRA TCASGSGQLT SCCSAAEVVS 
        60         70         80         90        100
VLFFHTMKYK QTDPEHPDND RFILSRGHAA PILYAAWVEV GDISESDLLN 
       110        120        130        140        150
LRKLHSDLER HPTPRLPFVD VATGSLGQGL GTACGMAYTG KYLDKASYRV 
       160        170        180        190        200
FCLMGDGESS EGSVWEAFAF ASHYNLDNLV AVFDVNRLGQ SGPAPLEHGA 
       210        220        230        240        250
DIYQNCCEAF GWNTYLVDGH DVEALCQAFW QASQVKNKPT AIVAKTFKGR 
       260        270        280        290        300
GIPNIEDAEN WHGKPVPKER ADAIVKLIES QIQTNENLIP KSPVEDSPQI 
       310        320        330        340        350
SITDIKMTSP PAYKVGDKIA TQKTYGLALA KLGRANERVI VLSGDTMNST 
       360        370        380        390        400
FSEIFRKEHP ERFIECIIAE QNMVSVALGC ATRGRTIAFA GAFAAFFTRA 
       410        420        430        440        450
FDQLRMGAIS QANINLIGSH CGVSTGEDGV SQMALEDLAM FRSIPNCTVF 
       460        470        480        490        500
YPSDAISTEH AIYLAANTKG MCFIRTSQPE TAVIYTPQEN FEIGQAKVVR 
       510        520        530        540        550
HGVNDKVTVI GAGVTLHEAL EAADHLSQQG ISVRVIDPFT IKPLDAATII 
       560        570        580        590        600
SSAKATGGRV ITVEDHYREG GIGEAVCAAV SREPDILVHQ LAVSGVPQRG 
       610        620 
KTSELLDMFG ISTRHIIAAV TLTLMK

Length:626

Mass (Da):67,877

Last modified:March 1, 2001 - v1

Checksum:ⁱ446C4EC81EDC0EA4

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	54	F → L in BAB71427 (PubMed:14702039).Curated	1
Sequence conflictⁱ	302	I → V in AAH28707 (PubMed:15489334).Curated	1
Sequence conflictⁱ	311	P → H in AAH28707 (PubMed:15489334).Curated	1
Sequence conflictⁱ	406	M → I in BAB71427 (PubMed:14702039).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_031990	442	R → Q. Corresponds to variant dbSNP:rs3811750Ensembl.		1
Natural variantⁱVAR_031991	590	Q → H1 PublicationCorresponds to variant dbSNP:rs11735477Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL136779 mRNA Translation: CAB66713.1 AK057325 mRNA Translation: BAB71427.1 CR533560 mRNA Translation: CAG38591.1 BC028707 mRNA Translation: AAH28707.4 BC125101 mRNA Translation: AAI25102.1 BC142943 mRNA Translation: AAI42944.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS3805.1
NCBI Reference Sequences More...RefSeqⁱ	NP_115512.3, NM_032136.4
UniGeneⁱ	Hs.303923

Genome annotation databases

Ensemblⁱ	ENST00000280605; ENSP00000280605; ENSG00000151005
GeneIDⁱ	84076
KEGGⁱ	hsa:84076
UCSC genome browser More...UCSCⁱ	uc003iqp.5 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Protein	Similar proteins		Species	Score	Length	Source
Q9H0I9	Testis tissue sperm-binding protein Li 39a		HUMAN		626	UniRef100_Q9H0I9
cDNA FLJ32975 fis, clone TESTI2013832, moderately similar to Xenopus laevis transketolase mRNA		HUMAN		320
Uncharacterized protein DKFZp434B129 (Fragment)		HUMAN		190

Protein	Similar proteins		Species	Score	Length	Source
Q9H0I9	Testis tissue sperm-binding protein Li 39a		HUMAN		626	UniRef90_Q9H0I9
Transketolase like 2		PANTR		722
Uncharacterized protein		PANPA		722
Uncharacterized protein		RHIRO		722
Uncharacterized protein		RHIBE		722
+35

Protein	Similar proteins		Species	Score	Length	Source
Q9H0I9	Transketolase	)	HUMAN		623	UniRef50_P29401
Transketolase		RAT		623
Transketolase-like protein 2		BOVIN		626
Transketolase		MOUSE		623
Transketolase		PANTR		623
+439

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL136779 mRNA Translation: CAB66713.1 AK057325 mRNA Translation: BAB71427.1 CR533560 mRNA Translation: CAG38591.1 BC028707 mRNA Translation: AAH28707.4 BC125101 mRNA Translation: AAI25102.1 BC142943 mRNA Translation: AAI42944.1
CCDSⁱ	CCDS3805.1
RefSeqⁱ	NP_115512.3, NM_032136.4
UniGeneⁱ	Hs.303923

3D structure databases

ProteinModelPortalⁱ	Q9H0I9
SMRⁱ	Q9H0I9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	123871, 14 interactors
IntActⁱ	Q9H0I9, 1 interactor
STRINGⁱ	9606.ENSP00000280605

PTM databases

iPTMnetⁱ	Q9H0I9
PhosphoSitePlusⁱ	Q9H0I9

Polymorphism and mutation databases

BioMutaⁱ	TKTL2
DMDMⁱ	74717985

Proteomic databases

MaxQBⁱ	Q9H0I9
PaxDbⁱ	Q9H0I9
PeptideAtlasⁱ	Q9H0I9
PRIDEⁱ	Q9H0I9
ProteomicsDBⁱ	80285

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	84076
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000280605; ENSP00000280605; ENSG00000151005
GeneIDⁱ	84076
KEGGⁱ	hsa:84076
UCSCⁱ	uc003iqp.5 human

Organism-specific databases

CTDⁱ	84076
DisGeNETⁱ	84076
EuPathDBⁱ	HostDB:ENSG00000151005.4
GeneCardsⁱ	TKTL2
HGNCⁱ	HGNC:25313 TKTL2
neXtProtⁱ	NX_Q9H0I9
OpenTargetsⁱ	ENSG00000151005
PharmGKBⁱ	PA142670806
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0523 Eukaryota COG0021 LUCA
GeneTreeⁱ	ENSGT00390000005240
HOGENOMⁱ	HOG000243868
HOVERGENⁱ	HBG004036
InParanoidⁱ	Q9H0I9
KOⁱ	K00615
OMAⁱ	FADYMRG
OrthoDBⁱ	EOG091G03M5
PhylomeDBⁱ	Q9H0I9
TreeFamⁱ	TF313097

Enzyme and pathway databases

BRENDAⁱ	2.2.1.1 2681

BRENDAⁱ

2.2.1.1 2681

Miscellaneous databases

GeneWikiⁱ	TKTL2
GenomeRNAiⁱ	84076
Protein Ontology More...PROⁱ	PR:Q9H0I9

Gene expression databases

Bgeeⁱ	ENSG00000151005 Expressed in 15 organ(s), highest expression level in testis
CleanExⁱ	HS_TKTL2
ExpressionAtlasⁱ	Q9H0I9 baseline and differential
Genevisibleⁱ	Q9H0I9 HS

Family and domain databases

Gene3Dⁱ	3.40.50.920, 1 hit
InterProⁱ	View protein in InterPro IPR029061 THDP-binding IPR009014 Transketo_C/PFOR_II IPR005475 Transketolase-like_Pyr-bd IPR033248 Transketolase_C IPR005474 Transketolase_N
Pfamⁱ	View protein in Pfam PF02779 Transket_pyr, 1 hit PF02780 Transketolase_C, 1 hit PF00456 Transketolase_N, 1 hit
SMARTⁱ	View protein in SMART SM00861 Transket_pyr, 1 hit
SUPFAMⁱ	SSF52518 SSF52518, 2 hits SSF52922 SSF52922, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TKTL2_HUMAN
Accessionⁱ	Q9H0I9Primary (citable) accession number: Q9H0I9 Secondary accession number(s): A4FVB4, Q8NCT0, Q96M82
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
	Last sequence update:	March 1, 2001
	Last modified:	November 7, 2018
	This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations

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UniProtKB - Q9H0I9 (TKTL2_HUMAN)

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Transketolase-like protein 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

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<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

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Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

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Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ