UniProtKB - Q9H0I9 (TKTL2_HUMAN)
Protein
Transketolase-like protein 2
Gene
TKTL2
Organism
Homo sapiens (Human)
Status
Functioni
Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Plays a pivotal role in carcinogenesis.1 Publication
Catalytic activityi
- D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphateEC:2.2.1.1
Cofactori
Protein has several cofactor binding sites:- Mg2+By similarity, Ca2+By similarity, Mn2+By similarity, Co2+By similarityNote: Binds 1 Mg2+ ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+.By similarity
- thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 41 | Thiamine pyrophosphateBy similarity | 1 | |
Binding sitei | 78 | Thiamine pyrophosphateBy similarity | 1 | |
Metal bindingi | 156 | MagnesiumBy similarity | 1 | |
Binding sitei | 157 | Thiamine pyrophosphate; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 186 | MagnesiumBy similarity | 1 | |
Binding sitei | 186 | Thiamine pyrophosphateBy similarity | 1 | |
Metal bindingi | 188 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 248 | Thiamine pyrophosphateBy similarity | 1 | |
Binding sitei | 262 | SubstrateBy similarity | 1 | |
Binding sitei | 262 | Thiamine pyrophosphateBy similarity | 1 | |
Sitei | 262 | Important for catalytic activityBy similarity | 1 | |
Binding sitei | 349 | SubstrateBy similarity | 1 | |
Active sitei | 370 | Proton donorBy similarity | 1 | |
Binding sitei | 370 | Thiamine pyrophosphateBy similarity | 1 | |
Binding sitei | 396 | Thiamine pyrophosphateBy similarity | 1 | |
Binding sitei | 420 | SubstrateBy similarity | 1 | |
Binding sitei | 428 | SubstrateBy similarity | 1 | |
Binding sitei | 432 | Thiamine pyrophosphateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 124 – 126 | Thiamine pyrophosphateBy similarity | 3 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- thiamine pyrophosphate binding Source: GO_Central
- transketolase activity Source: GO_Central
Keywordsi
Molecular function | Transferase |
Ligand | Calcium, Magnesium, Metal-binding, Thiamine pyrophosphate |
Enzyme and pathway databases
BRENDAi | 2.2.1.1, 2681 |
PathwayCommonsi | Q9H0I9 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TKTL2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000151005.4 |
HGNCi | HGNC:25313, TKTL2 |
neXtProti | NX_Q9H0I9 |
Subcellular locationi
Other locations
- cytoplasm Source: LIFEdb
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 84076 |
OpenTargetsi | ENSG00000151005 |
PharmGKBi | PA142670806 |
Miscellaneous databases
Pharosi | Q9H0I9, Tdark |
Polymorphism and mutation databases
BioMutai | TKTL2 |
DMDMi | 74717985 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285200 | 1 – 626 | Transketolase-like protein 2Add BLAST | 626 |
Proteomic databases
jPOSTi | Q9H0I9 |
MassIVEi | Q9H0I9 |
MaxQBi | Q9H0I9 |
PaxDbi | Q9H0I9 |
PeptideAtlasi | Q9H0I9 |
PRIDEi | Q9H0I9 |
ProteomicsDBi | 80285 |
PTM databases
iPTMneti | Q9H0I9 |
PhosphoSitePlusi | Q9H0I9 |
Expressioni
Tissue specificityi
Overexpressed in hepatoma cancer cells.1 Publication
Gene expression databases
Bgeei | ENSG00000151005, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q9H0I9, baseline and differential |
Genevisiblei | Q9H0I9, HS |
Organism-specific databases
HPAi | ENSG00000151005, Tissue enriched (testis) |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 123871, 14 interactors |
IntActi | Q9H0I9, 5 interactors |
STRINGi | 9606.ENSP00000280605 |
Miscellaneous databases
RNActi | Q9H0I9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the transketolase family.Curated
Phylogenomic databases
eggNOGi | KOG0523, Eukaryota |
GeneTreei | ENSGT00940000161969 |
HOGENOMi | CLU_009227_3_0_1 |
InParanoidi | Q9H0I9 |
OMAi | GCAPMGY |
OrthoDBi | 354970at2759 |
PhylomeDBi | Q9H0I9 |
TreeFami | TF313097 |
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR033248, Transketolase_C IPR005474, Transketolase_N |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9H0I9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMANDAKPDV KTVQVLRDTA NRLRIHSIRA TCASGSGQLT SCCSAAEVVS
60 70 80 90 100
VLFFHTMKYK QTDPEHPDND RFILSRGHAA PILYAAWVEV GDISESDLLN
110 120 130 140 150
LRKLHSDLER HPTPRLPFVD VATGSLGQGL GTACGMAYTG KYLDKASYRV
160 170 180 190 200
FCLMGDGESS EGSVWEAFAF ASHYNLDNLV AVFDVNRLGQ SGPAPLEHGA
210 220 230 240 250
DIYQNCCEAF GWNTYLVDGH DVEALCQAFW QASQVKNKPT AIVAKTFKGR
260 270 280 290 300
GIPNIEDAEN WHGKPVPKER ADAIVKLIES QIQTNENLIP KSPVEDSPQI
310 320 330 340 350
SITDIKMTSP PAYKVGDKIA TQKTYGLALA KLGRANERVI VLSGDTMNST
360 370 380 390 400
FSEIFRKEHP ERFIECIIAE QNMVSVALGC ATRGRTIAFA GAFAAFFTRA
410 420 430 440 450
FDQLRMGAIS QANINLIGSH CGVSTGEDGV SQMALEDLAM FRSIPNCTVF
460 470 480 490 500
YPSDAISTEH AIYLAANTKG MCFIRTSQPE TAVIYTPQEN FEIGQAKVVR
510 520 530 540 550
HGVNDKVTVI GAGVTLHEAL EAADHLSQQG ISVRVIDPFT IKPLDAATII
560 570 580 590 600
SSAKATGGRV ITVEDHYREG GIGEAVCAAV SREPDILVHQ LAVSGVPQRG
610 620
KTSELLDMFG ISTRHIIAAV TLTLMK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | F → L in BAB71427 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 302 | I → V in AAH28707 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 311 | P → H in AAH28707 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 406 | M → I in BAB71427 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031990 | 442 | R → Q. Corresponds to variant dbSNP:rs3811750Ensembl. | 1 | |
Natural variantiVAR_031991 | 590 | Q → H1 PublicationCorresponds to variant dbSNP:rs11735477Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136779 mRNA Translation: CAB66713.1 AK057325 mRNA Translation: BAB71427.1 CR533560 mRNA Translation: CAG38591.1 BC028707 mRNA Translation: AAH28707.4 BC125101 mRNA Translation: AAI25102.1 BC142943 mRNA Translation: AAI42944.1 |
CCDSi | CCDS3805.1 |
RefSeqi | NP_115512.3, NM_032136.4 |
Genome annotation databases
Ensembli | ENST00000280605; ENSP00000280605; ENSG00000151005 |
GeneIDi | 84076 |
KEGGi | hsa:84076 |
UCSCi | uc003iqp.5, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136779 mRNA Translation: CAB66713.1 AK057325 mRNA Translation: BAB71427.1 CR533560 mRNA Translation: CAG38591.1 BC028707 mRNA Translation: AAH28707.4 BC125101 mRNA Translation: AAI25102.1 BC142943 mRNA Translation: AAI42944.1 |
CCDSi | CCDS3805.1 |
RefSeqi | NP_115512.3, NM_032136.4 |
3D structure databases
SMRi | Q9H0I9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123871, 14 interactors |
IntActi | Q9H0I9, 5 interactors |
STRINGi | 9606.ENSP00000280605 |
PTM databases
iPTMneti | Q9H0I9 |
PhosphoSitePlusi | Q9H0I9 |
Polymorphism and mutation databases
BioMutai | TKTL2 |
DMDMi | 74717985 |
Proteomic databases
jPOSTi | Q9H0I9 |
MassIVEi | Q9H0I9 |
MaxQBi | Q9H0I9 |
PaxDbi | Q9H0I9 |
PeptideAtlasi | Q9H0I9 |
PRIDEi | Q9H0I9 |
ProteomicsDBi | 80285 |
Protocols and materials databases
Antibodypediai | 28268, 134 antibodies |
DNASUi | 84076 |
Genome annotation databases
Ensembli | ENST00000280605; ENSP00000280605; ENSG00000151005 |
GeneIDi | 84076 |
KEGGi | hsa:84076 |
UCSCi | uc003iqp.5, human |
Organism-specific databases
CTDi | 84076 |
DisGeNETi | 84076 |
EuPathDBi | HostDB:ENSG00000151005.4 |
GeneCardsi | TKTL2 |
HGNCi | HGNC:25313, TKTL2 |
HPAi | ENSG00000151005, Tissue enriched (testis) |
neXtProti | NX_Q9H0I9 |
OpenTargetsi | ENSG00000151005 |
PharmGKBi | PA142670806 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0523, Eukaryota |
GeneTreei | ENSGT00940000161969 |
HOGENOMi | CLU_009227_3_0_1 |
InParanoidi | Q9H0I9 |
OMAi | GCAPMGY |
OrthoDBi | 354970at2759 |
PhylomeDBi | Q9H0I9 |
TreeFami | TF313097 |
Enzyme and pathway databases
BRENDAi | 2.2.1.1, 2681 |
PathwayCommonsi | Q9H0I9 |
Miscellaneous databases
BioGRID-ORCSi | 84076, 2 hits in 838 CRISPR screens |
GeneWikii | TKTL2 |
GenomeRNAii | 84076 |
Pharosi | Q9H0I9, Tdark |
PROi | PR:Q9H0I9 |
RNActi | Q9H0I9, protein |
Gene expression databases
Bgeei | ENSG00000151005, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q9H0I9, baseline and differential |
Genevisiblei | Q9H0I9, HS |
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR033248, Transketolase_C IPR005474, Transketolase_N |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TKTL2_HUMAN | |
Accessioni | Q9H0I9Primary (citable) accession number: Q9H0I9 Secondary accession number(s): A4FVB4, Q8NCT0, Q96M82 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations