UniProtKB - Q9H0F6 (SHRPN_HUMAN)
Sharpin
SHARPIN
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 348 – 377 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 30 |
GO - Molecular functioni
- identical protein binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- protein-containing complex binding Source: Ensembl
- ubiquitin binding Source: GO_Central
- ubiquitin-protein transferase activity Source: GO_Central
GO - Biological processi
- apoptotic nuclear changes Source: Ensembl
- brain development Source: Ensembl
- I-kappaB kinase/NF-kappaB signaling Source: Reactome
- keratinization Source: Ensembl
- mitochondrion organization Source: Ensembl
- negative regulation of inflammatory response Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein linear polyubiquitination Source: UniProtKB
- regulation of CD40 signaling pathway Source: UniProtKB
- regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Keywordsi
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9H0F6 |
Reactomei | R-HSA-5357905, Regulation of TNFR1 signaling R-HSA-5357956, TNFR1-induced NFkappaB signaling pathway R-HSA-6794361, Neurexins and neuroligins |
Names & Taxonomyi
Protein namesi | Recommended name: Sharpin1 PublicationAlternative name(s): Shank-associated RH domain-interacting protein Shank-interacting protein-like 1 Short name: hSIPL1 |
Gene namesi | ORF Names:PSEC0216 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25321, SHARPIN |
MIMi | 611885, gene |
neXtProti | NX_Q9H0F6 |
VEuPathDBi | HostDB:ENSG00000179526.16 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Other locations
- dendrite Source: Ensembl
- LUBAC complex Source: UniProtKB
- postsynaptic density Source: Ensembl
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Cell junction, Cytoplasm, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 114 | V → D: Abolishes homodimerization. 1 Publication | 1 | |
Mutagenesisi | 272 | I → A: Abolishes interaction with RNF31 and ability to mediate linear polyubiquitination. 1 Publication | 1 | |
Mutagenesisi | 354 | C → S: Abolishes binding to ubiquitin without affecting interaction with RNF31; when associated with S-357. 1 Publication | 1 | |
Mutagenesisi | 357 | C → S: Abolishes binding to ubiquitin without affecting interaction with RNF31; when associated with S-354. 1 Publication | 1 | |
Mutagenesisi | 358 – 359 | TF → LV: Abolishes binding to ubiquitin and ability to mediate linear polyubiquitination. 1 Publication | 2 | |
Mutagenesisi | 368 | C → S: Abolishes binding to ubiquitin without affecting interaction with RNF31; when associated with S-371. 1 Publication | 1 | |
Mutagenesisi | 371 | C → S: Abolishes binding to ubiquitin without affecting interaction with RNF31; when associated with S-368. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 81858 |
OpenTargetsi | ENSG00000179526 |
PharmGKBi | PA142670925 |
Miscellaneous databases
Pharosi | Q9H0F6, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4296109 |
Genetic variation databases
BioMutai | SHARPIN |
DMDMi | 74733523 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000280634 | 1 – 387 | SharpinAdd BLAST | 387 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 165 | PhosphoserineCombined sources | 1 | |
Modified residuei | 312 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9H0F6 |
jPOSTi | Q9H0F6 |
MassIVEi | Q9H0F6 |
MaxQBi | Q9H0F6 |
PaxDbi | Q9H0F6 |
PeptideAtlasi | Q9H0F6 |
PRIDEi | Q9H0F6 |
ProteomicsDBi | 80272 [Q9H0F6-1] 80273 [Q9H0F6-2] |
PTM databases
iPTMneti | Q9H0F6 |
PhosphoSitePlusi | Q9H0F6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000179526, Expressed in testis and 224 other tissues |
ExpressionAtlasi | Q9H0F6, baseline and differential |
Genevisiblei | Q9H0F6, HS |
Organism-specific databases
HPAi | ENSG00000179526, Tissue enhanced (testis) |
Interactioni
Subunit structurei
Monomer and homodimer (By similarity).
Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31 (PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22549881). LUBAC has a MW of approximately 600 kDa suggesting a heteromultimeric assembly of its subunits (PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22549881). Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner (PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22549881).
Interacts with EYA1, EYA2, SHANK1 and SHANK3 (via ANK repeats) (By similarity).
By similarity4 PublicationsBinary interactionsi
Hide detailsQ9H0F6
Isoform 2 [Q9H0F6-2]
GO - Molecular functioni
- identical protein binding Source: Ensembl
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- ubiquitin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 123608, 154 interactors |
CORUMi | Q9H0F6 |
DIPi | DIP-57842N |
IntActi | Q9H0F6, 68 interactors |
MINTi | Q9H0F6 |
STRINGi | 9606.ENSP00000381698 |
Chemistry databases
BindingDBi | Q9H0F6 |
Miscellaneous databases
RNActi | Q9H0F6, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9H0F6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 219 – 288 | Ubiquitin-likeAdd BLAST | 70 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 180 | Self-associationBy similarityAdd BLAST | 180 | |
Regioni | 175 – 310 | Interaction with SHANK1By similarityAdd BLAST | 136 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 348 – 377 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 30 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1815, Eukaryota |
GeneTreei | ENSGT00940000161574 |
HOGENOMi | CLU_014998_0_1_1 |
InParanoidi | Q9H0F6 |
OMAi | YTIRGPS |
PhylomeDBi | Q9H0F6 |
TreeFami | TF323486 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR026261, RBCK1/SHARPIN IPR031912, Sharpin_PH IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR22770:SF43, PTHR22770:SF43, 1 hit |
Pfami | View protein in Pfam PF16764, Sharpin_PH, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPPAGGAAA AASDLGSAAV LLAVHAAVRP LGAGPDAEAQ LRRLQLSADP
60 70 80 90 100
ERPGRFRLEL LGAGPGAVNL EWPLESVSYT IRGPTQHELQ PPPGGPGTLS
110 120 130 140 150
LHFLNPQEAQ RWAVLVRGAT VEGQNGSKSN SPPALGPEAC PVSLPSPPEA
160 170 180 190 200
STLKGPPPEA DLPRSPGNLT EREELAGSLA RAIAGGDEKG AAQVAAVLAQ
210 220 230 240 250
HRVALSVQLQ EACFPPGPIR LQVTLEDAAS AASAASSAHV ALQVHPHCTV
260 270 280 290 300
AALQEQVFSE LGFPPAVQRW VIGRCLCVPE RSLASYGVRQ DGDPAFLYLL
310 320 330 340 350
SAPREAPATG PSPQHPQKMD GELGRLFPPS LGLPPGPQPA ASSLPSPLQP
360 370 380
SWSCPSCTFI NAPDRPGCEM CSTQRPCTWD PLAAAST
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0YCU2 | H0YCU2_HUMAN | Sharpin | SHARPIN | 95 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 238 | A → P in AAH02688 (Ref. 6) Curated | 1 | |
Sequence conflicti | 243 | Q → R in BAC11666 (PubMed:11230166).Curated | 1 | |
Sequence conflicti | 257 | V → A in AAH02688 (Ref. 6) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047799 | 282 | S → T. Corresponds to variant dbSNP:rs11541804Ensembl. | 1 | |
Natural variantiVAR_047800 | 294 | P → S. Corresponds to variant dbSNP:rs34674752EnsemblClinVar. | 1 | |
Natural variantiVAR_047801 | 311 | P → R. Corresponds to variant dbSNP:rs35844464Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023837 | 309 – 326 | TGPSP…ELGRL → QLVLSFLHLHQCPRPPWL in isoform 2. 2 PublicationsAdd BLAST | 18 | |
Alternative sequenceiVSP_023838 | 327 – 387 | Missing in isoform 2. 2 PublicationsAdd BLAST | 61 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB052764 mRNA Translation: BAC53797.1 Frameshift. AB052765 mRNA Translation: BAC53798.1 Sequence problems. FJ655995 mRNA Translation: ACN38058.1 AL136816 mRNA Translation: CAB66750.1 AK075518 mRNA Translation: BAC11666.1 Frameshift. AC104592 Genomic DNA No translation available. CH471162 Genomic DNA Translation: EAW82152.1 CH471162 Genomic DNA Translation: EAW82153.1 CH471162 Genomic DNA Translation: EAW82150.1 CH471162 Genomic DNA Translation: EAW82151.1 BC002688 mRNA Translation: AAH02688.2 BC025244 mRNA Translation: AAH25244.1 BC034028 mRNA Translation: AAH34028.1 |
CCDSi | CCDS43777.1 [Q9H0F6-1] |
RefSeqi | NP_112236.3, NM_030974.3 [Q9H0F6-1] XP_016869377.1, XM_017013888.1 [Q9H0F6-2] |
Genome annotation databases
Ensembli | ENST00000359551; ENSP00000352551; ENSG00000179526 [Q9H0F6-2] ENST00000398712; ENSP00000381698; ENSG00000179526 [Q9H0F6-1] |
GeneIDi | 81858 |
KEGGi | hsa:81858 |
UCSCi | uc003zba.4, human [Q9H0F6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB052764 mRNA Translation: BAC53797.1 Frameshift. AB052765 mRNA Translation: BAC53798.1 Sequence problems. FJ655995 mRNA Translation: ACN38058.1 AL136816 mRNA Translation: CAB66750.1 AK075518 mRNA Translation: BAC11666.1 Frameshift. AC104592 Genomic DNA No translation available. CH471162 Genomic DNA Translation: EAW82152.1 CH471162 Genomic DNA Translation: EAW82153.1 CH471162 Genomic DNA Translation: EAW82150.1 CH471162 Genomic DNA Translation: EAW82151.1 BC002688 mRNA Translation: AAH02688.2 BC025244 mRNA Translation: AAH25244.1 BC034028 mRNA Translation: AAH34028.1 |
CCDSi | CCDS43777.1 [Q9H0F6-1] |
RefSeqi | NP_112236.3, NM_030974.3 [Q9H0F6-1] XP_016869377.1, XM_017013888.1 [Q9H0F6-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4EMO | X-ray | 2.00 | A/B/C/D | 1-127 | [»] | |
5X0W | X-ray | 3.00 | B/D/F/H | 206-309 | [»] | |
SMRi | Q9H0F6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123608, 154 interactors |
CORUMi | Q9H0F6 |
DIPi | DIP-57842N |
IntActi | Q9H0F6, 68 interactors |
MINTi | Q9H0F6 |
STRINGi | 9606.ENSP00000381698 |
Chemistry databases
BindingDBi | Q9H0F6 |
ChEMBLi | CHEMBL4296109 |
PTM databases
iPTMneti | Q9H0F6 |
PhosphoSitePlusi | Q9H0F6 |
Genetic variation databases
BioMutai | SHARPIN |
DMDMi | 74733523 |
Proteomic databases
EPDi | Q9H0F6 |
jPOSTi | Q9H0F6 |
MassIVEi | Q9H0F6 |
MaxQBi | Q9H0F6 |
PaxDbi | Q9H0F6 |
PeptideAtlasi | Q9H0F6 |
PRIDEi | Q9H0F6 |
ProteomicsDBi | 80272 [Q9H0F6-1] 80273 [Q9H0F6-2] |
Protocols and materials databases
Antibodypediai | 7700, 246 antibodies |
Genome annotation databases
Ensembli | ENST00000359551; ENSP00000352551; ENSG00000179526 [Q9H0F6-2] ENST00000398712; ENSP00000381698; ENSG00000179526 [Q9H0F6-1] |
GeneIDi | 81858 |
KEGGi | hsa:81858 |
UCSCi | uc003zba.4, human [Q9H0F6-1] |
Organism-specific databases
CTDi | 81858 |
DisGeNETi | 81858 |
GeneCardsi | SHARPIN |
HGNCi | HGNC:25321, SHARPIN |
HPAi | ENSG00000179526, Tissue enhanced (testis) |
MIMi | 611885, gene |
neXtProti | NX_Q9H0F6 |
OpenTargetsi | ENSG00000179526 |
PharmGKBi | PA142670925 |
VEuPathDBi | HostDB:ENSG00000179526.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1815, Eukaryota |
GeneTreei | ENSGT00940000161574 |
HOGENOMi | CLU_014998_0_1_1 |
InParanoidi | Q9H0F6 |
OMAi | YTIRGPS |
PhylomeDBi | Q9H0F6 |
TreeFami | TF323486 |
Enzyme and pathway databases
PathwayCommonsi | Q9H0F6 |
Reactomei | R-HSA-5357905, Regulation of TNFR1 signaling R-HSA-5357956, TNFR1-induced NFkappaB signaling pathway R-HSA-6794361, Neurexins and neuroligins |
Miscellaneous databases
BioGRID-ORCSi | 81858, 13 hits in 993 CRISPR screens |
ChiTaRSi | SHARPIN, human |
GenomeRNAii | 81858 |
Pharosi | Q9H0F6, Tbio |
PROi | PR:Q9H0F6 |
RNActi | Q9H0F6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000179526, Expressed in testis and 224 other tissues |
ExpressionAtlasi | Q9H0F6, baseline and differential |
Genevisiblei | Q9H0F6, HS |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR026261, RBCK1/SHARPIN IPR031912, Sharpin_PH IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR22770:SF43, PTHR22770:SF43, 1 hit |
Pfami | View protein in Pfam PF16764, Sharpin_PH, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SHRPN_HUMAN | |
Accessioni | Q9H0F6Primary (citable) accession number: Q9H0F6 Secondary accession number(s): A6NEG3 Q9BUE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references