UniProtKB - Q9H0F5 (RNF38_HUMAN)
Protein
E3 ubiquitin-protein ligase RNF38
Gene
RNF38
Organism
Homo sapiens (Human)
Status
Functioni
Acts as an E3 ubiquitin-protein ligase able to ubiquitinate p53/TP53 which promotes its relocalization to discrete foci associated with PML nuclear bodies. Exhibits preference for UBE2D2 as a E2 enzyme.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 463 – 504 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: FlyBase
GO - Biological processi
- male gonad development Source: Ensembl
- protein ubiquitination Source: FlyBase
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9H0F5 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF38 (EC:2.3.2.27)Alternative name(s): RING finger protein 38 RING-type E3 ubiquitin transferase RNF38Curated |
Gene namesi | Name:RNF38 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18052, RNF38 |
MIMi | 612488, gene |
neXtProti | NX_Q9H0F5 |
VEuPathDBi | HostDB:ENSG00000137075.17 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: FlyBase
Other locations
- sperm flagellum Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 152006 |
OpenTargetsi | ENSG00000137075 |
PharmGKBi | PA34438 |
Miscellaneous databases
Pharosi | Q9H0F5, Tdark |
Genetic variation databases
BioMutai | RNF38 |
DMDMi | 56749664 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056078 | 1 – 515 | E3 ubiquitin-protein ligase RNF38Add BLAST | 515 |
Proteomic databases
PaxDbi | Q9H0F5 |
PeptideAtlasi | Q9H0F5 |
PRIDEi | Q9H0F5 |
ProteomicsDBi | 3188 80269 [Q9H0F5-1] 80270 [Q9H0F5-2] 80271 [Q9H0F5-3] 81080 |
PTM databases
iPTMneti | Q9H0F5 |
PhosphoSitePlusi | Q9H0F5 |
Expressioni
Tissue specificityi
Widely expressed with highest levels in testis.1 Publication
Gene expression databases
Bgeei | ENSG00000137075, Expressed in sperm and 237 other tissues |
Genevisiblei | Q9H0F5, HS |
Organism-specific databases
HPAi | ENSG00000137075, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsQ9H0F5
Isoform 2 [Q9H0F5-2]
With | #Exp. | IntAct |
---|---|---|
ATXN1 [P54253] | 6 | EBI-25866807,EBI-930964 |
CCT5 [P48643] | 3 | EBI-25866807,EBI-355710 |
HTT [P42858] | 21 | EBI-25866807,EBI-466029 |
WFS1 [O76024] | 3 | EBI-25866807,EBI-720609 |
Protein-protein interaction databases
BioGRIDi | 127416, 39 interactors |
IntActi | Q9H0F5, 28 interactors |
MINTi | Q9H0F5 |
STRINGi | 9606.ENSP00000259605 |
Miscellaneous databases
RNActi | Q9H0F5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9H0F5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9H0F5 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 57 – 71 | Bipartite nuclear localization signal 1Add BLAST | 15 | |
Motifi | 115 – 131 | Bipartite nuclear localization signal 2Add BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 239 – 404 | Pro-richAdd BLAST | 166 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 463 – 504 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000156228 |
HOGENOMi | CLU_024578_0_0_1 |
InParanoidi | Q9H0F5 |
OMAi | RSGYYQQ |
OrthoDBi | 1570520at2759 |
PhylomeDBi | Q9H0F5 |
TreeFami | TF325756 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9H0F5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MACKISPGAN SASLPGHPNK VICERVRLQS LFPLLPSDQN TTVQEDAHFK
60 70 80 90 100
AFFQSEDSPS PKRQRLSHSV FDYTSASPAP SPPMRPWEMT SNRQPPSVRP
110 120 130 140 150
SQHHFSGERC NTPARNRRSP PVRRQRGRRD RLSRHNSISQ DENYHHLPYA
160 170 180 190 200
QQQAIEEPRA FHPPNVSPRL LHPAAHPPQQ NAVMVDIHDQ LHQGTVPVSY
210 220 230 240 250
TVTTVAPHGI PLCTGQHIPA CSTQQVPGCS VVFSGQHLPV CSVPPPMLQA
260 270 280 290 300
CSVQHLPVPY AAFPPLISSD PFLIHPPHLS PHHPPHLPPP GQFVPFQTQQ
310 320 330 340 350
SRSPLQRIEN EVELLGEHLP VGGFTYPPSA HPPTLPPSAP LQFLTHDPLH
360 370 380 390 400
QEVSFGVPYP PFMPRRLTGR SRYRSQQPIP PPPYHPSLLP YVLSMLPVPP
410 420 430 440 450
AVGPTFSFEL DVEDGEVENY EALLNLAERL GEAKPRGLTK ADIEQLPSYR
460 470 480 490 500
FNPNNHQSEQ TLCVVCMCDF ESRQLLRVLP CNHEFHAKCV DKWLKANRTC
510
PICRADASEV HRDSE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 308 | I → K in CAB66751 (PubMed:11230166).Curated | 1 | |
Sequence conflicti | 421 | E → G in BAG52726 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055400 | 206 | A → T1 PublicationCorresponds to variant dbSNP:rs183475137Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053845 | 1 – 183 | Missing in isoform 5. 1 PublicationAdd BLAST | 183 | |
Alternative sequenceiVSP_037337 | 1 – 83 | Missing in isoform 3. 1 PublicationAdd BLAST | 83 | |
Alternative sequenceiVSP_053846 | 1 – 44 | MACKI…NTTVQ → MQHTCTQQKKVTYKHIARFS PRNRLCQADAVFNNNLAGFQ LQSP in isoform 4. 1 PublicationAdd BLAST | 44 | |
Alternative sequenceiVSP_012243 | 5 – 54 | Missing in isoform 2. 1 PublicationAdd BLAST | 50 | |
Alternative sequenceiVSP_053847 | 45 – 120 | Missing in isoform 4. 1 PublicationAdd BLAST | 76 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF394047 mRNA Translation: AAM73697.1 AL136817 mRNA Translation: CAB66751.3 AK024996 mRNA Translation: BAB15050.1 AK093480 mRNA Translation: BAG52726.1 AL161792 Genomic DNA No translation available. AL354935 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58305.1 BC033786 mRNA Translation: AAH33786.2 |
CCDSi | CCDS6603.1 [Q9H0F5-1] CCDS6604.1 [Q9H0F5-2] |
RefSeqi | NP_073618.3, NM_022781.4 [Q9H0F5-1] NP_919309.1, NM_194328.2 [Q9H0F5-3] NP_919310.1, NM_194329.2 [Q9H0F5-2] NP_919311.1, NM_194330.2 [Q9H0F5-3] NP_919313.1, NM_194332.2 [Q9H0F5-3] XP_005251423.1, XM_005251366.3 [Q9H0F5-3] XP_005251424.1, XM_005251367.3 [Q9H0F5-3] XP_005251425.1, XM_005251368.3 XP_006716784.1, XM_006716721.3 [Q9H0F5-3] XP_011516014.1, XM_011517712.2 [Q9H0F5-3] XP_011516015.1, XM_011517713.2 [Q9H0F5-3] XP_016869784.1, XM_017014295.1 |
Genome annotation databases
Ensembli | ENST00000259605; ENSP00000259605; ENSG00000137075 [Q9H0F5-1] ENST00000350199; ENSP00000343947; ENSG00000137075 [Q9H0F5-3] ENST00000353739; ENSP00000335239; ENSG00000137075 [Q9H0F5-2] ENST00000357058; ENSP00000349566; ENSG00000137075 [Q9H0F5-3] ENST00000377877; ENSP00000367109; ENSG00000137075 [Q9H0F5-4] ENST00000377885; ENSP00000367117; ENSG00000137075 [Q9H0F5-3] ENST00000611646; ENSP00000483536; ENSG00000137075 [Q9H0F5-4] |
GeneIDi | 152006 |
KEGGi | hsa:152006 |
UCSCi | uc003zzh.5, human [Q9H0F5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF394047 mRNA Translation: AAM73697.1 AL136817 mRNA Translation: CAB66751.3 AK024996 mRNA Translation: BAB15050.1 AK093480 mRNA Translation: BAG52726.1 AL161792 Genomic DNA No translation available. AL354935 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58305.1 BC033786 mRNA Translation: AAH33786.2 |
CCDSi | CCDS6603.1 [Q9H0F5-1] CCDS6604.1 [Q9H0F5-2] |
RefSeqi | NP_073618.3, NM_022781.4 [Q9H0F5-1] NP_919309.1, NM_194328.2 [Q9H0F5-3] NP_919310.1, NM_194329.2 [Q9H0F5-2] NP_919311.1, NM_194330.2 [Q9H0F5-3] NP_919313.1, NM_194332.2 [Q9H0F5-3] XP_005251423.1, XM_005251366.3 [Q9H0F5-3] XP_005251424.1, XM_005251367.3 [Q9H0F5-3] XP_005251425.1, XM_005251368.3 XP_006716784.1, XM_006716721.3 [Q9H0F5-3] XP_011516014.1, XM_011517712.2 [Q9H0F5-3] XP_011516015.1, XM_011517713.2 [Q9H0F5-3] XP_016869784.1, XM_017014295.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X4J | NMR | - | A | 445-506 | [»] | |
4V3K | X-ray | 2.04 | C/F | 439-515 | [»] | |
4V3L | X-ray | 1.53 | C | 439-515 | [»] | |
SMRi | Q9H0F5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127416, 39 interactors |
IntActi | Q9H0F5, 28 interactors |
MINTi | Q9H0F5 |
STRINGi | 9606.ENSP00000259605 |
PTM databases
iPTMneti | Q9H0F5 |
PhosphoSitePlusi | Q9H0F5 |
Genetic variation databases
BioMutai | RNF38 |
DMDMi | 56749664 |
Proteomic databases
PaxDbi | Q9H0F5 |
PeptideAtlasi | Q9H0F5 |
PRIDEi | Q9H0F5 |
ProteomicsDBi | 3188 80269 [Q9H0F5-1] 80270 [Q9H0F5-2] 80271 [Q9H0F5-3] 81080 |
Protocols and materials databases
Antibodypediai | 2887, 87 antibodies |
DNASUi | 152006 |
Genome annotation databases
Organism-specific databases
CTDi | 152006 |
DisGeNETi | 152006 |
GeneCardsi | RNF38 |
HGNCi | HGNC:18052, RNF38 |
HPAi | ENSG00000137075, Low tissue specificity |
MIMi | 612488, gene |
neXtProti | NX_Q9H0F5 |
OpenTargetsi | ENSG00000137075 |
PharmGKBi | PA34438 |
VEuPathDBi | HostDB:ENSG00000137075.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000156228 |
HOGENOMi | CLU_024578_0_0_1 |
InParanoidi | Q9H0F5 |
OMAi | RSGYYQQ |
OrthoDBi | 1570520at2759 |
PhylomeDBi | Q9H0F5 |
TreeFami | TF325756 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q9H0F5 |
Miscellaneous databases
BioGRID-ORCSi | 152006, 2 hits in 992 CRISPR screens |
ChiTaRSi | RNF38, human |
EvolutionaryTracei | Q9H0F5 |
GeneWikii | RNF38 |
GenomeRNAii | 152006 |
Pharosi | Q9H0F5, Tdark |
PROi | PR:Q9H0F5 |
RNActi | Q9H0F5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000137075, Expressed in sperm and 237 other tissues |
Genevisiblei | Q9H0F5, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNF38_HUMAN | |
Accessioni | Q9H0F5Primary (citable) accession number: Q9H0F5 Secondary accession number(s): A6PVP9 Q9H748 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | December 21, 2004 | |
Last modified: | April 7, 2021 | |
This is version 163 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references