UniProtKB - Q9H0E7 (UBP44_HUMAN)
Protein
Ubiquitin carboxyl-terminal hydrolase 44
Gene
USP44
Organism
Homo sapiens (Human)
Status
Functioni
Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.2 Publications
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).3 Publications EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 282 | Nucleophile | 1 | |
Active sitei | 636 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 27 – 88 | UBP-typePROSITE-ProRule annotationAdd BLAST | 62 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome segregation Source: Ensembl
- negative regulation of ubiquitin protein ligase activity Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein deubiquitination Source: UniProtKB
- regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Cell cycle, Cell division, Mitosis, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9H0E7 |
Reactomei | R-HSA-5689880, Ub-specific processing proteases |
Protein family/group databases
MEROPSi | C19.057 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 44 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 44 Ubiquitin thioesterase 44 Ubiquitin-specific-processing protease 44 |
Gene namesi | Name:USP44 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20064, USP44 |
MIMi | 610993, gene |
neXtProti | NX_Q9H0E7 |
VEuPathDBi | HostDB:ENSG00000136014.11 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Peaks in interphase, with relatively low levels maintained throughout mitosis.By similarity
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 282 | C → S or A: Abolishes deubiquitinase activity. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 84101 |
OpenTargetsi | ENSG00000136014 |
PharmGKBi | PA134931457 |
Miscellaneous databases
Pharosi | Q9H0E7, Tbio |
Genetic variation databases
BioMutai | USP44 |
DMDMi | 300669621 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080673 | 1 – 712 | Ubiquitin carboxyl-terminal hydrolase 44Add BLAST | 712 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 169 | Phosphoserine1 Publication | 1 | |
Modified residuei | 239 | Phosphoserine1 Publication | 1 | |
Modified residuei | 269 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 401 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Dephosphorylated by CTDP1.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome.1 Publication
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9H0E7 |
jPOSTi | Q9H0E7 |
MassIVEi | Q9H0E7 |
PaxDbi | Q9H0E7 |
PeptideAtlasi | Q9H0E7 |
PRIDEi | Q9H0E7 |
ProteomicsDBi | 80264 |
PTM databases
iPTMneti | Q9H0E7 |
PhosphoSitePlusi | Q9H0E7 |
Expressioni
Tissue specificityi
Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia.2 Publications
Developmental stagei
Elevated in mitosis; levels increase in mitotic cells and rapidly decrease once cells have completed chromosome attachment and exit from mitosis.1 Publication
Inductioni
Transcriptionally regulated by POU5F1/OCT4 in embryonic stem cells and embryonal carcinoma cells.1 Publication
Gene expression databases
Bgeei | ENSG00000136014, Expressed in secondary oocyte and 123 other tissues |
ExpressionAtlasi | Q9H0E7, baseline and differential |
Genevisiblei | Q9H0E7, HS |
Organism-specific databases
HPAi | ENSG00000136014, Tissue enhanced (testis) |
Interactioni
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 123890, 24 interactors |
IntActi | Q9H0E7, 12 interactors |
STRINGi | 9606.ENSP00000258499 |
Miscellaneous databases
RNActi | Q9H0E7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 273 – 678 | USPAdd BLAST | 406 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 27 – 88 | UBP-typePROSITE-ProRule annotationAdd BLAST | 62 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1867, Eukaryota |
GeneTreei | ENSGT00940000160526 |
HOGENOMi | CLU_008279_13_1_1 |
InParanoidi | Q9H0E7 |
OMAi | YCCRESL |
OrthoDBi | 1230415at2759 |
PhylomeDBi | Q9H0E7 |
TreeFami | TF315281 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
Pfami | View protein in Pfam PF00443, UCH, 1 hit PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00290, ZnF_UBP, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit PS50271, ZF_UBP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9H0E7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAMDTCKHV GQLQLAQDHS SLNPQKWHCV DCNTTESIWA CLSCSHVACG
60 70 80 90 100
RYIEEHALKH FQESSHPVAL EVNEMYVFCY LCDDYVLNDN TTGDLKLLRR
110 120 130 140 150
TLSAIKSQNY HCTTRSGRFL RSMGTGDDSY FLHDGAQSLL QSEDQLYTAL
160 170 180 190 200
WHRRRILMGK IFRTWFEQSP IGRKKQEEPF QEKIVVKREV KKRRQELEYQ
210 220 230 240 250
VKAELESMPP RKSLRLQGLA QSTIIEIVSV QVPAQTPASP AKDKVLSTSE
260 270 280 290 300
NEISQKVSDS SVKRRPIVTP GVTGLRNLGN TCYMNSVLQV LSHLLIFRQC
310 320 330 340 350
FLKLDLNQWL AMTASEKTRS CKHPPVTDTV VYQMNECQEK DTGFVCSRQS
360 370 380 390 400
SLSSGLSGGA SKGRKMELIQ PKEPTSQYIS LCHELHTLFQ VMWSGKWALV
410 420 430 440 450
SPFAMLHSVW RLIPAFRGYA QQDAQEFLCE LLDKIQRELE TTGTSLPALI
460 470 480 490 500
PTSQRKLIKQ VLNVVNNIFH GQLLSQVTCL ACDNKSNTIE PFWDLSLEFP
510 520 530 540 550
ERYQCSGKDI ASQPCLVTEM LAKFTETEAL EGKIYVCDQC NSKRRRFSSK
560 570 580 590 600
PVVLTEAQKQ LMICHLPQVL RLHLKRFRWS GRNNREKIGV HVGFEEILNM
610 620 630 640 650
EPYCCRETLK SLRPECFIYD LSAVVMHHGK GFGSGHYTAY CYNSEGGFWV
660 670 680 690 700
HCNDSKLSMC TMDEVCKAQA YILFYTQRVT ENGHSKLLPP ELLLGSQHPN
710
EDADTSSNEI LS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VRI7 | F8VRI7_HUMAN | Ubiquitinyl hydrolase 1 | USP44 | 496 | Annotation score: | ||
F8VRW0 | F8VRW0_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP44 | 164 | Annotation score: | ||
F8VVD6 | F8VVD6_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP44 | 111 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017125 | 91 | T → A3 PublicationsCorresponds to variant dbSNP:rs3812813Ensembl. | 1 | |
Natural variantiVAR_057043 | 316 | E → Q. Corresponds to variant dbSNP:rs7305024Ensembl. | 1 | |
Natural variantiVAR_057044 | 348 | R → G. Corresponds to variant dbSNP:rs7135642EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136825 mRNA Translation: CAB66759.1 AK315697 mRNA Translation: BAG38060.1 AC018475 Genomic DNA No translation available. BC030704 mRNA Translation: AAH30704.1 |
CCDSi | CCDS9053.1 |
RefSeqi | NP_001035862.1, NM_001042403.2 NP_001265322.1, NM_001278393.1 NP_001334865.1, NM_001347936.1 NP_001334866.1, NM_001347937.1 NP_115523.2, NM_032147.4 XP_016875499.1, XM_017020010.1 |
Genome annotation databases
Ensembli | ENST00000258499; ENSP00000258499; ENSG00000136014 ENST00000393091; ENSP00000376806; ENSG00000136014 ENST00000537435; ENSP00000442629; ENSG00000136014 |
GeneIDi | 84101 |
KEGGi | hsa:84101 |
UCSCi | uc001teg.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136825 mRNA Translation: CAB66759.1 AK315697 mRNA Translation: BAG38060.1 AC018475 Genomic DNA No translation available. BC030704 mRNA Translation: AAH30704.1 |
CCDSi | CCDS9053.1 |
RefSeqi | NP_001035862.1, NM_001042403.2 NP_001265322.1, NM_001278393.1 NP_001334865.1, NM_001347936.1 NP_001334866.1, NM_001347937.1 NP_115523.2, NM_032147.4 XP_016875499.1, XM_017020010.1 |
3D structure databases
SMRi | Q9H0E7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123890, 24 interactors |
IntActi | Q9H0E7, 12 interactors |
STRINGi | 9606.ENSP00000258499 |
Protein family/group databases
MEROPSi | C19.057 |
PTM databases
iPTMneti | Q9H0E7 |
PhosphoSitePlusi | Q9H0E7 |
Genetic variation databases
BioMutai | USP44 |
DMDMi | 300669621 |
Proteomic databases
EPDi | Q9H0E7 |
jPOSTi | Q9H0E7 |
MassIVEi | Q9H0E7 |
PaxDbi | Q9H0E7 |
PeptideAtlasi | Q9H0E7 |
PRIDEi | Q9H0E7 |
ProteomicsDBi | 80264 |
Protocols and materials databases
Antibodypediai | 17590, 273 antibodies |
DNASUi | 84101 |
Genome annotation databases
Ensembli | ENST00000258499; ENSP00000258499; ENSG00000136014 ENST00000393091; ENSP00000376806; ENSG00000136014 ENST00000537435; ENSP00000442629; ENSG00000136014 |
GeneIDi | 84101 |
KEGGi | hsa:84101 |
UCSCi | uc001teg.5, human |
Organism-specific databases
CTDi | 84101 |
DisGeNETi | 84101 |
GeneCardsi | USP44 |
HGNCi | HGNC:20064, USP44 |
HPAi | ENSG00000136014, Tissue enhanced (testis) |
MIMi | 610993, gene |
neXtProti | NX_Q9H0E7 |
OpenTargetsi | ENSG00000136014 |
PharmGKBi | PA134931457 |
VEuPathDBi | HostDB:ENSG00000136014.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1867, Eukaryota |
GeneTreei | ENSGT00940000160526 |
HOGENOMi | CLU_008279_13_1_1 |
InParanoidi | Q9H0E7 |
OMAi | YCCRESL |
OrthoDBi | 1230415at2759 |
PhylomeDBi | Q9H0E7 |
TreeFami | TF315281 |
Enzyme and pathway databases
PathwayCommonsi | Q9H0E7 |
Reactomei | R-HSA-5689880, Ub-specific processing proteases |
Miscellaneous databases
BioGRID-ORCSi | 84101, 8 hits in 994 CRISPR screens |
ChiTaRSi | USP44, human |
GeneWikii | USP44 |
GenomeRNAii | 84101 |
Pharosi | Q9H0E7, Tbio |
PROi | PR:Q9H0E7 |
RNActi | Q9H0E7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000136014, Expressed in secondary oocyte and 123 other tissues |
ExpressionAtlasi | Q9H0E7, baseline and differential |
Genevisiblei | Q9H0E7, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
Pfami | View protein in Pfam PF00443, UCH, 1 hit PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00290, ZnF_UBP, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit PS50271, ZF_UBP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP44_HUMAN | |
Accessioni | Q9H0E7Primary (citable) accession number: Q9H0E7 Secondary accession number(s): B2RDW3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | July 13, 2010 | |
Last modified: | April 7, 2021 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families