UniProtKB - Q9H093 (NUAK2_HUMAN)
Protein
NUAK family SNF1-like kinase 2
Gene
NUAK2
Organism
Homo sapiens (Human)
Status
Functioni
Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.4 Publications
Catalytic activityi
Cofactori
Mg2+2 Publications
Activity regulationi
Activated by phosphorylation on Thr-208.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 81 | ATPPROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 175 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 59 – 67 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- apoptotic process Source: UniProtKB-KW
- cellular response to glucose starvation Source: UniProtKB
- intracellular signal transduction Source: GO_Central
- negative regulation of apoptotic process Source: UniProtKB
- protein phosphorylation Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9H093 |
SignaLinki | Q9H093 |
SIGNORi | Q9H093 |
Names & Taxonomyi
Protein namesi | Recommended name: NUAK family SNF1-like kinase 2 (EC:2.7.11.1)Alternative name(s): Omphalocele kinase 2 SNF1/AMP kinase-related kinase Short name: SNARK |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29558, NUAK2 |
MIMi | 608131, gene |
neXtProti | NX_Q9H093 |
VEuPathDBi | HostDB:ENSG00000163545.8 |
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 81 | K → R: Loss of autophosphorylation, kinase activity and of anti-apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 208 | T → A: Prevents phosphorylation and activation by STK11/LKB1 complex. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 81788 |
OpenTargetsi | ENSG00000163545 |
PharmGKBi | PA142671243 |
Miscellaneous databases
Pharosi | Q9H093, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5698 |
DrugBanki | DB11936, Bempedoic acid DB12010, Fostamatinib |
DrugCentrali | Q9H093 |
GuidetoPHARMACOLOGYi | 2130 |
Genetic variation databases
BioMutai | NUAK2 |
DMDMi | 74761376 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247756 | 1 – 628 | NUAK family SNF1-like kinase 2Add BLAST | 628 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 208 | Phosphothreonine; by LKB11 Publication | 1 | |
Modified residuei | 435 | PhosphoserineCombined sources | 1 | |
Modified residuei | 523 | PhosphoserineCombined sources | 1 | |
Modified residuei | 544 | PhosphoserineCombined sources | 1 | |
Modified residuei | 547 | PhosphoserineCombined sources | 1 | |
Modified residuei | 573 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9H093 |
jPOSTi | Q9H093 |
MassIVEi | Q9H093 |
MaxQBi | Q9H093 |
PaxDbi | Q9H093 |
PeptideAtlasi | Q9H093 |
PRIDEi | Q9H093 |
ProteomicsDBi | 80220 |
PTM databases
iPTMneti | Q9H093 |
PhosphoSitePlusi | Q9H093 |
Expressioni
Gene expression databases
Bgeei | ENSG00000163545, Expressed in ectocervix and 175 other tissues |
ExpressionAtlasi | Q9H093, baseline and differential |
Genevisiblei | Q9H093, HS |
Organism-specific databases
HPAi | ENSG00000163545, Tissue enhanced (blood) |
Interactioni
Binary interactionsi
Q9H093
With | #Exp. | IntAct |
---|---|---|
GRIPAP1 [Q4V328] | 3 | EBI-1181722,EBI-717919 |
Protein-protein interaction databases
BioGRIDi | 123581, 19 interactors |
IntActi | Q9H093, 16 interactors |
STRINGi | 9606.ENSP00000356125 |
Chemistry databases
BindingDBi | Q9H093 |
Miscellaneous databases
RNActi | Q9H093, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 53 – 303 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 251 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0611, Eukaryota |
GeneTreei | ENSGT00940000158422 |
HOGENOMi | CLU_000288_63_42_1 |
InParanoidi | Q9H093 |
OrthoDBi | 1539664at2759 |
PhylomeDBi | Q9H093 |
TreeFami | TF324572 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9H093-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESLVFARRS GPTPSAAELA RPLAEGLIKS PKPLMKKQAV KRHHHKHNLR
60 70 80 90 100
HRYEFLETLG KGTYGKVKKA RESSGRLVAI KSIRKDKIKD EQDLMHIRRE
110 120 130 140 150
IEIMSSLNHP HIIAIHEVFE NSSKIVIVME YASRGDLYDY ISERQQLSER
160 170 180 190 200
EARHFFRQIV SAVHYCHQNR VVHRDLKLEN ILLDANGNIK IADFGLSNLY
210 220 230 240 250
HQGKFLQTFC GSPLYASPEI VNGKPYTGPE VDSWSLGVLL YILVHGTMPF
260 270 280 290 300
DGHDHKILVK QISNGAYREP PKPSDACGLI RWLLMVNPTR RATLEDVASH
310 320 330 340 350
WWVNWGYATR VGEQEAPHEG GHPGSDSARA SMADWLRRSS RPLLENGAKV
360 370 380 390 400
CSFFKQHAPG GGSTTPGLER QHSLKKSRKE NDMAQSLHSD TADDTAHRPG
410 420 430 440 450
KSNLKLPKGI LKKKVSASAE GVQEDPPELS PIPASPGQAA PLLPKKGILK
460 470 480 490 500
KPRQRESGYY SSPEPSESGE LLDAGDVFVS GDPKEQKPPQ ASGLLLHRKG
510 520 530 540 550
ILKLNGKFSQ TALELAAPTT FGSLDELAPP RPLARASRPS GAVSEDSILS
560 570 580 590 600
SESFDQLDLP ERLPEPPLRG CVSVDNLTGL EEPPSEGPGS CLRRWRQDPL
610 620
GDSCFSLTDC QEVTATYRQA LRVCSKLT
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_040964 | 309 | T → S1 PublicationCorresponds to variant dbSNP:rs55745939Ensembl. | 1 | |
Natural variantiVAR_040965 | 341 | R → L1 PublicationCorresponds to variant dbSNP:rs35208615Ensembl. | 1 | |
Natural variantiVAR_040966 | 503 | K → R in an ovarian Endometrioid carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1271546767Ensembl. | 1 | |
Natural variantiVAR_040967 | 516 | A → V1 PublicationCorresponds to variant dbSNP:rs35070935Ensembl. | 1 | |
Natural variantiVAR_040968 | 541 | G → E in a breast pleomorphic lobular carcinoma sample; somatic mutation. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136891 mRNA Translation: CAB66825.1 AK074830 mRNA Translation: BAC11234.1 BC017306 mRNA Translation: AAH17306.1 |
CCDSi | CCDS1453.2 |
RefSeqi | NP_112214.2, NM_030952.2 |
Genome annotation databases
Ensembli | ENST00000367157; ENSP00000356125; ENSG00000163545 |
GeneIDi | 81788 |
KEGGi | hsa:81788 |
UCSCi | uc001hce.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136891 mRNA Translation: CAB66825.1 AK074830 mRNA Translation: BAC11234.1 BC017306 mRNA Translation: AAH17306.1 |
CCDSi | CCDS1453.2 |
RefSeqi | NP_112214.2, NM_030952.2 |
3D structure databases
SMRi | Q9H093 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123581, 19 interactors |
IntActi | Q9H093, 16 interactors |
STRINGi | 9606.ENSP00000356125 |
Chemistry databases
BindingDBi | Q9H093 |
ChEMBLi | CHEMBL5698 |
DrugBanki | DB11936, Bempedoic acid DB12010, Fostamatinib |
DrugCentrali | Q9H093 |
GuidetoPHARMACOLOGYi | 2130 |
PTM databases
iPTMneti | Q9H093 |
PhosphoSitePlusi | Q9H093 |
Genetic variation databases
BioMutai | NUAK2 |
DMDMi | 74761376 |
Proteomic databases
EPDi | Q9H093 |
jPOSTi | Q9H093 |
MassIVEi | Q9H093 |
MaxQBi | Q9H093 |
PaxDbi | Q9H093 |
PeptideAtlasi | Q9H093 |
PRIDEi | Q9H093 |
ProteomicsDBi | 80220 |
Protocols and materials databases
Antibodypediai | 2090, 262 antibodies |
DNASUi | 81788 |
Genome annotation databases
Ensembli | ENST00000367157; ENSP00000356125; ENSG00000163545 |
GeneIDi | 81788 |
KEGGi | hsa:81788 |
UCSCi | uc001hce.4, human |
Organism-specific databases
CTDi | 81788 |
DisGeNETi | 81788 |
GeneCardsi | NUAK2 |
HGNCi | HGNC:29558, NUAK2 |
HPAi | ENSG00000163545, Tissue enhanced (blood) |
MIMi | 608131, gene |
neXtProti | NX_Q9H093 |
OpenTargetsi | ENSG00000163545 |
PharmGKBi | PA142671243 |
VEuPathDBi | HostDB:ENSG00000163545.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0611, Eukaryota |
GeneTreei | ENSGT00940000158422 |
HOGENOMi | CLU_000288_63_42_1 |
InParanoidi | Q9H093 |
OrthoDBi | 1539664at2759 |
PhylomeDBi | Q9H093 |
TreeFami | TF324572 |
Enzyme and pathway databases
PathwayCommonsi | Q9H093 |
SignaLinki | Q9H093 |
SIGNORi | Q9H093 |
Miscellaneous databases
BioGRID-ORCSi | 81788, 3 hits in 903 CRISPR screens |
ChiTaRSi | NUAK2, human |
GeneWikii | NUAK2 |
GenomeRNAii | 81788 |
Pharosi | Q9H093, Tchem |
PROi | PR:Q9H093 |
RNActi | Q9H093, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163545, Expressed in ectocervix and 175 other tissues |
ExpressionAtlasi | Q9H093, baseline and differential |
Genevisiblei | Q9H093, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUAK2_HUMAN | |
Accessioni | Q9H093Primary (citable) accession number: Q9H093 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families