NUAK2

Functionⁱ

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.4 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactorⁱ

Mg²⁺2 Publications

Enzyme regulationⁱ

Activated by phosphorylation on Thr-208.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	81	ATPPROSITE-ProRule annotation1 Publication		1
Active siteⁱ	175	Proton acceptorPROSITE-ProRule annotationBy similarity		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	59 – 67	ATPPROSITE-ProRule annotationBy similarity		9

GO - Molecular functionⁱ

ATP binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14976552
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 14976552
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 14976552
- 19927127

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin cytoskeleton organization
Source: UniProtKB
Inferred from direct assayⁱ
- 14575707
apoptotic process Source: UniProtKB-KW
cellular response to glucose starvation
Source: UniProtKB
Inferred from direct assayⁱ
- 14575707
intracellular signal transduction Source: GO_Central
negative regulation of apoptotic process
Source: UniProtKB
Inferred from direct assayⁱ
- 15345718
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 14976552

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Apoptosis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkⁱ	Q9H093
SIGNORⁱ	Q9H093

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: NUAK family SNF1-like kinase 2 (EC:2.7.11.1) Alternative name(s): Omphalocele kinase 2 SNF1/AMP kinase-related kinase Short name: SNARK
Gene namesⁱ	Name:NUAK2Imported Synonyms:OMPHK2, SNARK
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000163545.8
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:29558 NUAK2
MIMⁱ	608131 gene
neXtProtⁱ	NX_Q9H093

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	81	K → R: Loss of autophosphorylation, kinase activity and of anti-apoptotic activity. 1 Publication		1
Mutagenesisⁱ	208	T → A: Prevents phosphorylation and activation by STK11/LKB1 complex. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	81788
PharmGKBⁱ	PA142671243

Chemistry databases

ChEMBLⁱ	CHEMBL5698
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2130

Polymorphism and mutation databases

BioMutaⁱ	NUAK2
DMDMⁱ	74761376

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000247756	1 – 628	NUAK family SNF1-like kinase 2Add BLAST		628

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources	1
Modified residueⁱ	208	Phosphothreonine; by LKB11 Publication	1
Modified residueⁱ	435	PhosphoserineCombined sources	1
Modified residueⁱ	523	PhosphoserineCombined sources	1
Modified residueⁱ	544	PhosphoserineCombined sources	1
Modified residueⁱ	547	PhosphoserineCombined sources	1
Modified residueⁱ	573	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro.1 Publication

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q9H093
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9H093
PaxDbⁱ	Q9H093
PeptideAtlas More...PeptideAtlasⁱ	Q9H093
PRIDEⁱ	Q9H093
ProteomicsDBⁱ	80220

PTM databases

iPTMnetⁱ	Q9H093
PhosphoSitePlusⁱ	Q9H093

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000163545
CleanExⁱ	HS_NUAK2
ExpressionAtlasⁱ	Q9H093 baseline and differential
Genevisibleⁱ	Q9H093 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA008958

HPAⁱ

HPA008958

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	123581, 17 interactors
IntActⁱ	Q9H093, 4 interactors
STRINGⁱ	9606.ENSP00000356125

Chemistry databases

BindingDBⁱ

Q9H093

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9H093
SMRⁱ	Q9H093
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	53 – 303	Protein kinasePROSITE-ProRule annotationAdd BLAST		251

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0611 Eukaryota ENOG410XPDM LUCA
HOGENOMⁱ	HOG000004864
HOVERGENⁱ	HBG007160
InParanoidⁱ	Q9H093
KEGG Orthology (KO) More...KOⁱ	K08800
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G033W
PhylomeDBⁱ	Q9H093
TreeFamⁱ	TF324572

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9H093-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MESLVFARRS GPTPSAAELA RPLAEGLIKS PKPLMKKQAV KRHHHKHNLR 
        60         70         80         90        100
HRYEFLETLG KGTYGKVKKA RESSGRLVAI KSIRKDKIKD EQDLMHIRRE 
       110        120        130        140        150
IEIMSSLNHP HIIAIHEVFE NSSKIVIVME YASRGDLYDY ISERQQLSER 
       160        170        180        190        200
EARHFFRQIV SAVHYCHQNR VVHRDLKLEN ILLDANGNIK IADFGLSNLY 
       210        220        230        240        250
HQGKFLQTFC GSPLYASPEI VNGKPYTGPE VDSWSLGVLL YILVHGTMPF 
       260        270        280        290        300
DGHDHKILVK QISNGAYREP PKPSDACGLI RWLLMVNPTR RATLEDVASH 
       310        320        330        340        350
WWVNWGYATR VGEQEAPHEG GHPGSDSARA SMADWLRRSS RPLLENGAKV 
       360        370        380        390        400
CSFFKQHAPG GGSTTPGLER QHSLKKSRKE NDMAQSLHSD TADDTAHRPG 
       410        420        430        440        450
KSNLKLPKGI LKKKVSASAE GVQEDPPELS PIPASPGQAA PLLPKKGILK 
       460        470        480        490        500
KPRQRESGYY SSPEPSESGE LLDAGDVFVS GDPKEQKPPQ ASGLLLHRKG 
       510        520        530        540        550
ILKLNGKFSQ TALELAAPTT FGSLDELAPP RPLARASRPS GAVSEDSILS 
       560        570        580        590        600
SESFDQLDLP ERLPEPPLRG CVSVDNLTGL EEPPSEGPGS CLRRWRQDPL 
       610        620 
GDSCFSLTDC QEVTATYRQA LRVCSKLT

Length:628

Mass (Da):69,612

Last modified:March 1, 2001 - v1

Checksum:ⁱF76F8B1BF94F4C87

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_040964	309	T → S1 PublicationCorresponds to variant dbSNP:rs55745939Ensembl.	1
Natural variantⁱVAR_040965	341	R → L1 PublicationCorresponds to variant dbSNP:rs35208615Ensembl.	1
Natural variantⁱVAR_040966	503	K → R in an ovarian Endometrioid carcinoma sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_040967	516	A → V1 PublicationCorresponds to variant dbSNP:rs35070935Ensembl.	1
Natural variantⁱVAR_040968	541	G → E in a breast pleomorphic lobular carcinoma sample; somatic mutation. 1 Publication	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL136891 mRNA Translation: CAB66825.1 AK074830 mRNA Translation: BAC11234.1 BC017306 mRNA Translation: AAH17306.1
NCBI Reference Sequences More...RefSeqⁱ	NP_112214.2, NM_030952.2
UniGeneⁱ	Hs.497512

Genome annotation databases

Ensemblⁱ	ENST00000367157; ENSP00000356125; ENSG00000163545
GeneIDⁱ	81788
KEGGⁱ	hsa:81788
UCSC genome browser More...UCSCⁱ	uc001hce.4 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Protein	Similar proteins		Species	Score	Length
Q9H093	cDNA FLJ56013, highly similar to NUAK family SNF1-like kinase 2 (EC 2.7.11.1)		HUMAN		672
NUAK family kinase 2		MACMU		672
NUAK family kinase 2		PANTR		672
NUAK family kinase 2		CERAT		672
NUAK family kinase 2		RHIRO		672
+24

Protein	Similar proteins		Species	Score	Length
Q9H093	cDNA FLJ56013, highly similar to NUAK family SNF1-like kinase 2 (EC 2.7.11.1)		HUMAN		672
NUAK family kinase 2		RHIRO		672
NUAK family kinase 2		RHIBE		672
NUAK family kinase 2		PANPA		672
NUAK family kinase 2		CHLSB		672
+155

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL136891 mRNA Translation: CAB66825.1 AK074830 mRNA Translation: BAC11234.1 BC017306 mRNA Translation: AAH17306.1
NCBI Reference Sequences More...RefSeqⁱ	NP_112214.2, NM_030952.2
UniGeneⁱ	Hs.497512

3D structure databases

ProteinModelPortalⁱ	Q9H093
SMRⁱ	Q9H093
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	123581, 17 interactors
IntActⁱ	Q9H093, 4 interactors
STRINGⁱ	9606.ENSP00000356125

Chemistry databases

BindingDBⁱ	Q9H093
ChEMBLⁱ	CHEMBL5698
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2130

PTM databases

iPTMnetⁱ	Q9H093
PhosphoSitePlusⁱ	Q9H093

Polymorphism and mutation databases

BioMutaⁱ	NUAK2
DMDMⁱ	74761376

Proteomic databases

EPDⁱ	Q9H093
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9H093
PaxDbⁱ	Q9H093
PeptideAtlas More...PeptideAtlasⁱ	Q9H093
PRIDEⁱ	Q9H093
ProteomicsDBⁱ	80220

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	81788
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000367157; ENSP00000356125; ENSG00000163545
GeneIDⁱ	81788
KEGGⁱ	hsa:81788
UCSC genome browser More...UCSCⁱ	uc001hce.4 human

Organism-specific databases

CTDⁱ	81788
DisGeNET More...DisGeNETⁱ	81788
EuPathDBⁱ	HostDB:ENSG00000163545.8
GeneCardsⁱ	NUAK2
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:29558 NUAK2
Human Protein Atlas More...HPAⁱ	HPA008958
MIMⁱ	608131 gene
neXtProtⁱ	NX_Q9H093
PharmGKBⁱ	PA142671243
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0611 Eukaryota ENOG410XPDM LUCA
HOGENOMⁱ	HOG000004864
HOVERGENⁱ	HBG007160
InParanoidⁱ	Q9H093
KEGG Orthology (KO) More...KOⁱ	K08800
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G033W
PhylomeDBⁱ	Q9H093
TreeFamⁱ	TF324572

Enzyme and pathway databases

SignaLinkⁱ	Q9H093
SIGNORⁱ	Q9H093

Miscellaneous databases

GeneWikiⁱ	NUAK2
GenomeRNAiⁱ	81788
Protein Ontology More...PROⁱ	PR:Q9H093
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000163545
CleanExⁱ	HS_NUAK2
ExpressionAtlasⁱ	Q9H093 baseline and differential
Genevisibleⁱ	Q9H093 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NUAK2_HUMAN
Accessionⁱ	Q9H093Primary (citable) accession number: Q9H093
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
	Last sequence update:	March 1, 2001
	Last modified:	June 20, 2018
	This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human and mouse protein kinases
Human and mouse protein kinases: classification and index
SIMILARITY comments
Index of protein domains and families

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Supporting data

UniProtKB - Q9H093 (NUAK2_HUMAN)

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NUAK family SNF1-like kinase 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Enzyme regulationⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ