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Protein

SprT-like domain-containing protein Spartan

Gene

SPRTN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri453 – 476UBZ-typeAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-110320 Translesion Synthesis by POLH

Names & Taxonomyi

Protein namesi
Recommended name:
SprT-like domain-containing protein Spartan
Alternative name(s):
DNA damage protein targeting VCP
Short name:
DVC1
Protein with SprT-like domain at the N terminus
Short name:
Spartan
Gene namesi
Name:SPRTN
Synonyms:C1orf124, DVC1
ORF Names:UNQ1880/PRO4323
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000010072.15
HGNCiHGNC:25356 SPRTN
MIMi616086 gene
neXtProtiNX_Q9H040

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Involvement in diseasei

Ruijs-Aalfs syndrome (RJALS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by genomic instability, progeroid features, and susceptibility toward early onset hepatocellular carcinoma.
See also OMIM:616200
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072708117Y → C in RJALS; cells ectopically expressing the mutant are completely unable to restore DNA replication fork progression. 1 PublicationCorresponds to variant dbSNP:rs527236213EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi253F → A: Abolishes binding to VCP/p97; when associated with A-260. 1 Publication1
Mutagenesisi260L → A: Abolishes binding to VCP/p97; when associated with A-253. 1 Publication1
Mutagenesisi325 – 332QNVLSNYF → ANVASNAA: Abolishes binding to PCNA. 1 Publication8
Mutagenesisi331 – 332YF → AA: Abolishes binding to PCNA. 2 Publications2
Mutagenesisi456 – 459CPVC → APVA: Abolishes binding to ubiquitin. 3 Publications4
Mutagenesisi473D → A: Abolishes binding to ubiquitin. 1 Publication1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi83932
MalaCardsiSPRTN
MIMi616200 phenotype
OpenTargetsiENSG00000010072
PharmGKBiPA142672442

Polymorphism and mutation databases

BioMutaiSPRTN
DMDMi162416221

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003127481 – 489SprT-like domain-containing protein SpartanAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki341Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki361Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki376Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki423Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki484Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9H040
MaxQBiQ9H040
PaxDbiQ9H040
PeptideAtlasiQ9H040
PRIDEiQ9H040
ProteomicsDBi80202
80203 [Q9H040-2]

PTM databases

iPTMnetiQ9H040
PhosphoSitePlusiQ9H040

Expressioni

Developmental stagei

Predominantly expressed during S- and G2-phases and early M-phase. It then drops, and is probably degraded by the APC/C complex.1 Publication

Gene expression databases

BgeeiENSG00000010072 Expressed in 177 organ(s), highest expression level in testis
CleanExiHS_C1orf124
ExpressionAtlasiQ9H040 baseline and differential
GenevisibleiQ9H040 HS

Organism-specific databases

HPAiHPA025073

Interactioni

Subunit structurei

Interacts with PCNA (when ubiquitinated). Interacts with RAD18. Interacts (via its SHP-box) with VCP/p97. Interacts with KCTD13 and POLD3.6 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123817, 97 interactors
IntActiQ9H040, 4 interactors
STRINGi9606.ENSP00000295050

Structurei

Secondary structure

1489
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9H040
SMRiQ9H040
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 212SprT-likeAdd BLAST168

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi253 – 261SHP-box9
Motifi325 – 332PIP-box8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi444 – 451Poly-Ser8

Domaini

The PIP-box mediates the interaction with PCNA, while the UBZ-type zinc finger mediates binding to 'Lys-48'- and 'Lys-63'-linked polyubiquitin (PubMed:22894931, PubMed:22681887, PubMed:23042607, PubMed:23042605 and PubMed:22987070).3 Publications

Sequence similaritiesi

Belongs to the Spartan family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri453 – 476UBZ-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3931 Eukaryota
ENOG410XPXU LUCA
GeneTreeiENSGT00390000003585
HOGENOMiHOG000031496
HOVERGENiHBG101206
InParanoidiQ9H040
OMAiNEHLDWC
OrthoDBiEOG091G02ZI
PhylomeDBiQ9H040
TreeFamiTF314762

Family and domain databases

InterProiView protein in InterPro
IPR006640 SprT-like_domain
IPR006642 Znf_Rad18_put
PfamiView protein in Pfam
PF10263 SprT-like, 1 hit
SMARTiView protein in SMART
SM00731 SprT, 1 hit
SM00734 ZnF_Rad18, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H040-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDDDLMLALR LQEEWNLQEA ERDHAQESLS LVDASWELVD PTPDLQALFV
60 70 80 90 100
QFNDQFFWGQ LEAVEVKWSV RMTLCAGICS YEGKGGMCSI RLSEPLLKLR
110 120 130 140 150
PRKDLVETLL HEMIHAYLFV TNNDKDREGH GPEFCKHMHR INSLTGANIT
160 170 180 190 200
VYHTFHDEVD EYRRHWWRCN GPCQHRPPYY GYVKRATNRE PSAHDYWWAE
210 220 230 240 250
HQKTCGGTYI KIKEPENYSK KGKGKAKLGK EPVLAAENKD KPNRGEAQLV
260 270 280 290 300
IPFSGKGYVL GETSNLPSPG KLITSHAINK TQDLLNQNHS ANAVRPNSKI
310 320 330 340 350
KVKFEQNGSS KNSHLVSPAV SNSHQNVLSN YFPRVSFANQ KAFRGVNGSP
360 370 380 390 400
RISVTVGNIP KNSVSSSSQR RVSSSKISLR NSSKVTESAS VMPSQDVSGS
410 420 430 440 450
EDTFPNKRPR LEDKTVFDNF FIKKEQIKSS GNDPKYSTTT AQNSSSSSSQ
460 470 480
SKMVNCPVCQ NEVLESQINE HLDWCLEGDS IKVKSEESL
Length:489
Mass (Da):55,134
Last modified:December 4, 2007 - v2
Checksum:i9CF437C057B2BA2B
GO
Isoform 2 (identifier: Q9H040-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     240-250: DKPNRGEAQLV → GTFVYILLIFM
     251-489: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):29,191
Checksum:iF06A426627CF2473
GO
Isoform 3 (identifier: Q9H040-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-150: Missing.
     240-250: DKPNRGEAQLV → GTFVYILLIFM
     251-489: Missing.

Note: No experimental confirmation available.
Show »
Length:207
Mass (Da):24,247
Checksum:i18733A05FD49D4B1
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AKT1B1AKT1_HUMAN
SprT-like domain-containing protein...
SPRTN
187Annotation score:

Sequence cautioni

The sequence BAB55037 differs from that shown. Reason: Frameshift at position 224.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072708117Y → C in RJALS; cells ectopically expressing the mutant are completely unable to restore DNA replication fork progression. 1 PublicationCorresponds to variant dbSNP:rs527236213EnsemblClinVar.1
Natural variantiVAR_037556296P → L3 PublicationsCorresponds to variant dbSNP:rs2437150Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046925108 – 150Missing in isoform 3. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_029891240 – 250DKPNRGEAQLV → GTFVYILLIFM in isoform 2 and isoform 3. 3 PublicationsAdd BLAST11
Alternative sequenceiVSP_029892251 – 489Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST239

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358611 mRNA Translation: AAQ88974.1
AK027613 mRNA Translation: BAB55232.1
AK027317 mRNA Translation: BAB55037.1 Frameshift.
AL512744 mRNA Translation: CAC21670.1
AL117352 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW69956.1
BC015740 mRNA Translation: AAH15740.1
BC068478 mRNA Translation: AAH68478.1
CCDSiCCDS1594.1 [Q9H040-1]
CCDS31054.1 [Q9H040-2]
CCDS58066.1 [Q9H040-3]
RefSeqiNP_001010984.1, NM_001010984.3 [Q9H040-2]
NP_001248391.1, NM_001261462.2 [Q9H040-3]
NP_114407.3, NM_032018.6 [Q9H040-1]
UniGeneiHs.554892

Genome annotation databases

EnsembliENST00000008440; ENSP00000008440; ENSG00000010072 [Q9H040-3]
ENST00000295050; ENSP00000295050; ENSG00000010072 [Q9H040-1]
ENST00000391858; ENSP00000375731; ENSG00000010072 [Q9H040-2]
GeneIDi83932
KEGGihsa:83932
UCSCiuc001hur.5 human [Q9H040-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358611 mRNA Translation: AAQ88974.1
AK027613 mRNA Translation: BAB55232.1
AK027317 mRNA Translation: BAB55037.1 Frameshift.
AL512744 mRNA Translation: CAC21670.1
AL117352 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW69956.1
BC015740 mRNA Translation: AAH15740.1
BC068478 mRNA Translation: AAH68478.1
CCDSiCCDS1594.1 [Q9H040-1]
CCDS31054.1 [Q9H040-2]
CCDS58066.1 [Q9H040-3]
RefSeqiNP_001010984.1, NM_001010984.3 [Q9H040-2]
NP_001248391.1, NM_001261462.2 [Q9H040-3]
NP_114407.3, NM_032018.6 [Q9H040-1]
UniGeneiHs.554892

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY4X-ray2.94B/D/F321-336[»]
ProteinModelPortaliQ9H040
SMRiQ9H040
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123817, 97 interactors
IntActiQ9H040, 4 interactors
STRINGi9606.ENSP00000295050

PTM databases

iPTMnetiQ9H040
PhosphoSitePlusiQ9H040

Polymorphism and mutation databases

BioMutaiSPRTN
DMDMi162416221

Proteomic databases

EPDiQ9H040
MaxQBiQ9H040
PaxDbiQ9H040
PeptideAtlasiQ9H040
PRIDEiQ9H040
ProteomicsDBi80202
80203 [Q9H040-2]

Protocols and materials databases

DNASUi83932
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000008440; ENSP00000008440; ENSG00000010072 [Q9H040-3]
ENST00000295050; ENSP00000295050; ENSG00000010072 [Q9H040-1]
ENST00000391858; ENSP00000375731; ENSG00000010072 [Q9H040-2]
GeneIDi83932
KEGGihsa:83932
UCSCiuc001hur.5 human [Q9H040-1]

Organism-specific databases

CTDi83932
DisGeNETi83932
EuPathDBiHostDB:ENSG00000010072.15
GeneCardsiSPRTN
H-InvDBiHIX0001695
HGNCiHGNC:25356 SPRTN
HPAiHPA025073
MalaCardsiSPRTN
MIMi616086 gene
616200 phenotype
neXtProtiNX_Q9H040
OpenTargetsiENSG00000010072
PharmGKBiPA142672442
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3931 Eukaryota
ENOG410XPXU LUCA
GeneTreeiENSGT00390000003585
HOGENOMiHOG000031496
HOVERGENiHBG101206
InParanoidiQ9H040
OMAiNEHLDWC
OrthoDBiEOG091G02ZI
PhylomeDBiQ9H040
TreeFamiTF314762

Enzyme and pathway databases

ReactomeiR-HSA-110320 Translesion Synthesis by POLH

Miscellaneous databases

GeneWikiiC1orf124
GenomeRNAii83932
PROiPR:Q9H040
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000010072 Expressed in 177 organ(s), highest expression level in testis
CleanExiHS_C1orf124
ExpressionAtlasiQ9H040 baseline and differential
GenevisibleiQ9H040 HS

Family and domain databases

InterProiView protein in InterPro
IPR006640 SprT-like_domain
IPR006642 Znf_Rad18_put
PfamiView protein in Pfam
PF10263 SprT-like, 1 hit
SMARTiView protein in SMART
SM00731 SprT, 1 hit
SM00734 ZnF_Rad18, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPRTN_HUMAN
AccessioniPrimary (citable) accession number: Q9H040
Secondary accession number(s): B1AKT0
, B5MEF7, Q5TE78, Q6UWW6, Q96BC5, Q96KA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: September 12, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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