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Entry version 153 (07 Apr 2021)
Sequence version 2 (04 Dec 2007)
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Protein

DNA-dependent metalloprotease SPRTN

Gene

SPRTN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed:27852435, PubMed:27871366, PubMed:27871365, PubMed:32649882, PubMed:30893605). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed:27852435, PubMed:27871366, PubMed:27871365, PubMed:32649882). Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis (PubMed:27852435, PubMed:27871366, PubMed:27871365, PubMed:32649882). Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4 (PubMed:27871366). Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs (By similarity). SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs (By similarity). Involved in recruitment of VCP/p97 to sites of DNA damage (PubMed:22902628, PubMed:23042605, PubMed:23042607, PubMed:32152270). Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation (PubMed:31316063). Does not activate CHEK1 in response to DNA damage (PubMed:31316063). May also act as a 'reader' of ubiquitinated PCNA: recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA (PubMed:22681887, PubMed:22894931, PubMed:22902628, PubMed:22987070). Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis (PubMed:22681887).By similarity13 Publications

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

DNA-binding activates the protease activity: single-stranded DNA-binding specifically activates ability to cleave covalent DNA-protein cross-links (DPCs) (PubMed:27871366, PubMed:27871365, PubMed:30893605). In contrast, double-stranded DNA-binding specifically activates autocatalytic cleavage, and subsequent inactivation (PubMed:27852435, PubMed:27871365, PubMed:30893605).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi111Zinc; catalyticPROSITE-ProRule annotationCombined sources1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei112PROSITE-ProRule annotation4 Publications1
Metal bindingi115Zinc; catalyticPROSITE-ProRule annotationCombined sources1 Publication1
Metal bindingi130Zinc; catalyticPROSITE-ProRule annotationCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri453 – 480UBZ4-typePROSITE-ProRule annotationAdd BLAST28

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...
PathwayCommonsi
Q9H040

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-110320, Translesion Synthesis by POLH

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA-dependent metalloprotease SPRTNCurated (EC:3.4.24.-4 Publications)
Alternative name(s):
DNA damage protein targeting VCP1 Publication
Short name:
DVC11 Publication
Protein with SprT-like domain at the N terminus1 Publication
Short name:
Spartan1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SPRTN1 PublicationImported
Synonyms:C1orf1241 PublicationImported, DVC11 Publication
ORF Names:UNQ1880/PRO4323
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:25356, SPRTN

Online Mendelian Inheritance in Man (OMIM)

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MIMi
616086, gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_Q9H040

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
HostDB:ENSG00000010072.15

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Ruijs-Aalfs syndrome (RJALS)4 Publications
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by genomic instability, progeroid features, and susceptibility toward early onset hepatocellular carcinoma.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_072708117Y → C in RJALS; impaired metalloprotease activity and ability to cleave covalent DNA-protein cross-links (DPCs); cells are completely unable to restore DNA replication fork progression. 4 PublicationsCorresponds to variant dbSNP:rs527236213EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi71R → E: Abolished ability to mediate autocleavage. Does not affect DNA-binding. 1 Publication1
Mutagenesisi91R → E: Abolished ability to mediate autocleavage. Does not affect DNA-binding. 1 Publication1
Mutagenesisi112E → A or Q: Abolished metalloprotease activity and ability to cleave covalent DNA-protein cross-links (DPCs). Abolished ability to cleave CHEK1 during physiological DNA replication. 4 Publications1
Mutagenesisi149I → A: Abolished ability to mediate autocleavage in presence of ssDNA. 1 Publication1
Mutagenesisi153H → A: Abolished ability to mediate autocleavage in presence of ssDNA. 1 Publication1
Mutagenesisi155F → A: Abolished ability to mediate autocleavage in presence of ssDNA. 1 Publication1
Mutagenesisi156H → A: Abolished ability to mediate autocleavage in presence of ssDNA. 1 Publication1
Mutagenesisi157D → K: Increased ability to mediate autocleavage. 1 Publication1
Mutagenesisi159V → A: Abolished ability to mediate autocleavage in presence of ssDNA. 1 Publication1
Mutagenesisi163R → E: Abolished ability to mediate autocleavage. Strongly reduced DNA-binding. 1 Publication1
Mutagenesisi179Y → A: Abolished metalloprotease activity and ability to mediate autocleavage. Slightly impaired ability to cleave covalent DNA-protein cross-links (DPCs). 1 Publication1
Mutagenesisi185R → A: Abolished metalloprotease activity and ability to cleave covalent DNA-protein cross-links (DPCs). 1 Publication1
Mutagenesisi189R → E: Abolished ability to mediate autocleavage. Strongly reduced DNA-binding. 1 Publication1
Mutagenesisi194H → E: Strongly reduced ability to mediate autocleavage. Strongly reduced DNA-binding. 1 Publication1
Mutagenesisi197W → A: Abolished metalloprotease activity, ability to cleave covalent DNA-protein cross-links (DPCs) and to mediate autocleavage. 1 Publication1
Mutagenesisi211K → E: Strongly reduced ability to mediate autocleavage. Strongly reduced DNA-binding. 1 Publication1
Mutagenesisi230K → Q: Mimics acetylation, promoting cleavage of covalent DNA-protein cross-links (DPCs). 1 Publication1
Mutagenesisi230K → R: Abolished acetylation, leading to impaired localization to chromatin. 1 Publication1
Mutagenesisi253F → A: Abolishes binding to VCP/p97; when associated with A-260. 1 Publication1
Mutagenesisi260L → A: Abolishes binding to VCP/p97; when associated with A-253. 1 Publication1
Mutagenesisi325 – 332QNVLSNYF → ANVASNAA: Abolished interaction with PCNA. 1 Publication8
Mutagenesisi326N → V: Decreased interaction with PCNA. 1 Publication1
Mutagenesisi331 – 332YF → AA: Abolished interaction with PCNA. 2 Publications2
Mutagenesisi331 – 332YF → FY: Abolished interaction with PCNA. 1 Publication2
Mutagenesisi331Y → F: Abolished interaction with PCNA. 1 Publication1
Mutagenesisi332F → Y: Abolished interaction with PCNA. 1 Publication1
Mutagenesisi341K → R: Does not abolish monoubiquitination; when associated with R-376, R-414 and R-435. 1 Publication1
Mutagenesisi373S → A: Abolished phosphorylation by CHEK1; when associated with A-374 and A-383. 1 Publication1
Mutagenesisi374S → A: Abolished phosphorylation by CHEK1; when associated with A-373 and A-383. 1 Publication1
Mutagenesisi376K → R: Does not abolish monoubiquitination; when associated with R-341, R-414 and R-435. 1 Publication1
Mutagenesisi383S → A: Abolished phosphorylation by CHEK1; when associated with A-373 and A-374. 1 Publication1
Mutagenesisi408 – 411RPRL → APRA: Promotes relocalization to the cytoplasm. 1 Publication4
Mutagenesisi414K → R: Does not abolish monoubiquitination; when associated with R-341, R-376 and R-435. 1 Publication1
Mutagenesisi435K → R: Does not abolish monoubiquitination; when associated with R-341, R-376 and R-414. 1 Publication1
Mutagenesisi456 – 459CPVC → APVA: Abolishes binding to ubiquitin. 3 Publications4
Mutagenesisi473D → A: Abolishes binding to ubiquitin. 1 Publication1

Keywords - Diseasei

Disease variant

Organism-specific databases

DisGeNET

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DisGeNETi
83932

MalaCards human disease database

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MalaCardsi
SPRTN
MIMi616200, phenotype

Open Targets

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OpenTargetsi
ENSG00000010072

Orphanet; a database dedicated to information on rare diseases and orphan drugs

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Orphaneti
435953, Progeroid features-hepatocellular carcinoma predisposition syndrome

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA142672442

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

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Pharosi
Q9H040, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
SPRTN

Domain mapping of disease mutations (DMDM)

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DMDMi
162416221

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003127481 – 489DNA-dependent metalloprotease SPRTNAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1N-acetylmethionineCombined sources1
Modified residuei230N6-acetyllysine1 Publication1
Modified residuei268PhosphoserineCombined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki341Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki341Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Cross-linki361Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei373Phosphoserine; by CHEK11 Publication1
Modified residuei374Phosphoserine; by CHEK11 Publication1
Cross-linki376Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki376Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Modified residuei383Phosphoserine; by CHEK11 Publication1
Cross-linki414Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki423Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki435Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki484Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Autocatalytically cleaved in response to double-stranded DNA-binding: autocatalytic cleavage takes place in trans and leads to inactivation.4 Publications
Monoubiquitinated; monoubiquitination promotes exclusion from chromatin (PubMed:27871365, PubMed:32649882). Deubiquitinated by VCPIP1: deubiquitination is required for subsequent acetylation and recruitment to chromatin and DNA damage sites (PubMed:27871365, PubMed:32649882).2 Publications
Acetylated following deubiquitination by VCPIP1, leading to recruitment to chromatin and DNA damage sites.1 Publication
Phosphorylation by CHEK1 promotes recruitment to chromatin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei227 – 228Cleavage; by autolysis1 Publication2

Keywords - PTMi

Acetylation, Autocatalytic cleavage, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
Q9H040

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q9H040

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q9H040

MaxQB - The MaxQuant DataBase

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MaxQBi
Q9H040

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q9H040

PeptideAtlas

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PeptideAtlasi
Q9H040

PRoteomics IDEntifications database

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PRIDEi
Q9H040

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
3103
80202 [Q9H040-1]
80203 [Q9H040-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q9H040

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q9H040

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Predominantly expressed during S- and G2-phases and early M-phase (PubMed:23042605). It then drops, and is probably degraded by the APC/C complex (PubMed:23042605).1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000010072, Expressed in testis and 193 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q9H040, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q9H040, HS

Organism-specific databases

Human Protein Atlas

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HPAi
ENSG00000010072, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer (PubMed:30893605).

Interacts (VIA PIP-box) with PCNA (when ubiquitinated) (PubMed:22894931, PubMed:22902628, PubMed:22681887, PubMed:23042605, PubMed:23042607, PubMed:22987070, PubMed:27084448).

Interacts (via its SHP-box) with VCP/p97 (PubMed:22902628, PubMed:23042605, PubMed:23042607).

Interacts with RAD18 (PubMed:22681887).

Interacts with KCTD13 and POLD3 (PubMed:22902628).

8 Publications

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
123817, 100 interactors

Protein interaction database and analysis system

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IntActi
Q9H040, 11 interactors

STRING: functional protein association networks

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STRINGi
9606.ENSP00000295050

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
Q9H040, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1489
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9H040

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini45 – 212SprT-likeSequence analysisAdd BLAST168

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi253 – 261SHP-box1 Publication9
Motifi325 – 332PIP-box4 Publications8
Motifi402 – 413Nuclear localization signal1 PublicationAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi444 – 451Poly-Ser8

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PIP-box mediates the interaction with PCNA, while the UBZ4-type zinc finger mediates binding to 'Lys-48'- and 'Lys-63'-linked polyubiquitin.6 Publications

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Spartan family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri453 – 480UBZ4-typePROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3931, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000003585

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_083493_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q9H040

Identification of Orthologs from Complete Genome Data

More...
OMAi
AYLFITN

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9H040

TreeFam database of animal gene trees

More...
TreeFami
TF314762

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006642, Rad18_UBZ4
IPR044245, Spartan
IPR006640, SprT-like_domain

The PANTHER Classification System

More...
PANTHERi
PTHR21220, PTHR21220, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF10263, SprT-like, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00731, SprT, 1 hit
SM00734, ZnF_Rad18, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51908, ZF_UBZ4, 1 hit
PS00142, ZINC_PROTEASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9H040-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDDDLMLALR LQEEWNLQEA ERDHAQESLS LVDASWELVD PTPDLQALFV
60 70 80 90 100
QFNDQFFWGQ LEAVEVKWSV RMTLCAGICS YEGKGGMCSI RLSEPLLKLR
110 120 130 140 150
PRKDLVETLL HEMIHAYLFV TNNDKDREGH GPEFCKHMHR INSLTGANIT
160 170 180 190 200
VYHTFHDEVD EYRRHWWRCN GPCQHRPPYY GYVKRATNRE PSAHDYWWAE
210 220 230 240 250
HQKTCGGTYI KIKEPENYSK KGKGKAKLGK EPVLAAENKD KPNRGEAQLV
260 270 280 290 300
IPFSGKGYVL GETSNLPSPG KLITSHAINK TQDLLNQNHS ANAVRPNSKI
310 320 330 340 350
KVKFEQNGSS KNSHLVSPAV SNSHQNVLSN YFPRVSFANQ KAFRGVNGSP
360 370 380 390 400
RISVTVGNIP KNSVSSSSQR RVSSSKISLR NSSKVTESAS VMPSQDVSGS
410 420 430 440 450
EDTFPNKRPR LEDKTVFDNF FIKKEQIKSS GNDPKYSTTT AQNSSSSSSQ
460 470 480
SKMVNCPVCQ NEVLESQINE HLDWCLEGDS IKVKSEESL
Length:489
Mass (Da):55,134
Last modified:December 4, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9CF437C057B2BA2B
GO
Isoform 2 (identifier: Q9H040-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     240-250: DKPNRGEAQLV → GTFVYILLIFM
     251-489: Missing.

Show »
Length:250
Mass (Da):29,191
Checksum:iF06A426627CF2473
GO
Isoform 3 (identifier: Q9H040-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-150: Missing.
     240-250: DKPNRGEAQLV → GTFVYILLIFM
     251-489: Missing.

Show »
Length:207
Mass (Da):24,247
Checksum:i18733A05FD49D4B1
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AKT1B1AKT1_HUMAN
DNA-dependent metalloprotease SPRTN
SPRTN
187Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAB55037 differs from that shown. Reason: Frameshift.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072708117Y → C in RJALS; impaired metalloprotease activity and ability to cleave covalent DNA-protein cross-links (DPCs); cells are completely unable to restore DNA replication fork progression. 4 PublicationsCorresponds to variant dbSNP:rs527236213EnsemblClinVar.1
Natural variantiVAR_037556296P → L3 PublicationsCorresponds to variant dbSNP:rs2437150Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_046925108 – 150Missing in isoform 3. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_029891240 – 250DKPNRGEAQLV → GTFVYILLIFM in isoform 2 and isoform 3. 3 PublicationsAdd BLAST11
Alternative sequenceiVSP_029892251 – 489Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST239

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY358611 mRNA Translation: AAQ88974.1
AK027613 mRNA Translation: BAB55232.1
AK027317 mRNA Translation: BAB55037.1 Frameshift.
AL512744 mRNA Translation: CAC21670.1
AL117352 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW69956.1
BC015740 mRNA Translation: AAH15740.1
BC068478 mRNA Translation: AAH68478.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS1594.1 [Q9H040-1]
CCDS31054.1 [Q9H040-2]
CCDS58066.1 [Q9H040-3]

NCBI Reference Sequences

More...
RefSeqi
NP_001010984.1, NM_001010984.3 [Q9H040-2]
NP_001248391.1, NM_001261462.2 [Q9H040-3]
NP_114407.3, NM_032018.6 [Q9H040-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000008440; ENSP00000008440; ENSG00000010072 [Q9H040-3]
ENST00000295050; ENSP00000295050; ENSG00000010072 [Q9H040-1]
ENST00000391858; ENSP00000375731; ENSG00000010072 [Q9H040-2]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
83932

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:83932

UCSC genome browser

More...
UCSCi
uc001hur.5, human [Q9H040-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358611 mRNA Translation: AAQ88974.1
AK027613 mRNA Translation: BAB55232.1
AK027317 mRNA Translation: BAB55037.1 Frameshift.
AL512744 mRNA Translation: CAC21670.1
AL117352 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW69956.1
BC015740 mRNA Translation: AAH15740.1
BC068478 mRNA Translation: AAH68478.1
CCDSiCCDS1594.1 [Q9H040-1]
CCDS31054.1 [Q9H040-2]
CCDS58066.1 [Q9H040-3]
RefSeqiNP_001010984.1, NM_001010984.3 [Q9H040-2]
NP_001248391.1, NM_001261462.2 [Q9H040-3]
NP_114407.3, NM_032018.6 [Q9H040-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY4X-ray2.94B/D/F321-336[»]
6MDWX-ray1.50A26-214[»]
6MDXX-ray1.55A28-214[»]
SMRiQ9H040
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi123817, 100 interactors
IntActiQ9H040, 11 interactors
STRINGi9606.ENSP00000295050

PTM databases

iPTMnetiQ9H040
PhosphoSitePlusiQ9H040

Genetic variation databases

BioMutaiSPRTN
DMDMi162416221

Proteomic databases

EPDiQ9H040
jPOSTiQ9H040
MassIVEiQ9H040
MaxQBiQ9H040
PaxDbiQ9H040
PeptideAtlasiQ9H040
PRIDEiQ9H040
ProteomicsDBi3103
80202 [Q9H040-1]
80203 [Q9H040-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
20798, 129 antibodies

The DNASU plasmid repository

More...
DNASUi
83932

Genome annotation databases

EnsembliENST00000008440; ENSP00000008440; ENSG00000010072 [Q9H040-3]
ENST00000295050; ENSP00000295050; ENSG00000010072 [Q9H040-1]
ENST00000391858; ENSP00000375731; ENSG00000010072 [Q9H040-2]
GeneIDi83932
KEGGihsa:83932
UCSCiuc001hur.5, human [Q9H040-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
83932
DisGeNETi83932

GeneCards: human genes, protein and diseases

More...
GeneCardsi
SPRTN
HGNCiHGNC:25356, SPRTN
HPAiENSG00000010072, Low tissue specificity
MalaCardsiSPRTN
MIMi616086, gene
616200, phenotype
neXtProtiNX_Q9H040
OpenTargetsiENSG00000010072
Orphaneti435953, Progeroid features-hepatocellular carcinoma predisposition syndrome
PharmGKBiPA142672442
VEuPathDBiHostDB:ENSG00000010072.15

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG3931, Eukaryota
GeneTreeiENSGT00390000003585
HOGENOMiCLU_083493_0_0_1
InParanoidiQ9H040
OMAiAYLFITN
PhylomeDBiQ9H040
TreeFamiTF314762

Enzyme and pathway databases

PathwayCommonsiQ9H040
ReactomeiR-HSA-110320, Translesion Synthesis by POLH

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
83932, 573 hits in 967 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
SPRTN, human

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
C1orf124

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
83932
PharosiQ9H040, Tbio

Protein Ontology

More...
PROi
PR:Q9H040
RNActiQ9H040, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000010072, Expressed in testis and 193 other tissues
ExpressionAtlasiQ9H040, baseline and differential
GenevisibleiQ9H040, HS

Family and domain databases

InterProiView protein in InterPro
IPR006642, Rad18_UBZ4
IPR044245, Spartan
IPR006640, SprT-like_domain
PANTHERiPTHR21220, PTHR21220, 1 hit
PfamiView protein in Pfam
PF10263, SprT-like, 1 hit
SMARTiView protein in SMART
SM00731, SprT, 1 hit
SM00734, ZnF_Rad18, 1 hit
PROSITEiView protein in PROSITE
PS51908, ZF_UBZ4, 1 hit
PS00142, ZINC_PROTEASE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPRTN_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9H040
Secondary accession number(s): B1AKT0
, B5MEF7, Q5TE78, Q6UWW6, Q96BC5, Q96KA0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: April 7, 2021
This is version 153 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
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