UniProtKB - Q9GZV8 (PRD14_HUMAN)
Protein
PR domain zinc finger protein 14
Gene
PRDM14
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.By similarity2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 400 – 424 | C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 432 – 455 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 461 – 483 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 489 – 511 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 517 – 540 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 546 – 568 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- chromatin DNA binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- methyltransferase activity Source: UniProtKB-KW
- RNA binding Source: Ensembl
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- cell fate specification Source: Ensembl
- cell morphogenesis Source: Ensembl
- embryo implantation Source: Ensembl
- fertilization Source: Ensembl
- germ cell development Source: Ensembl
- germ-line stem cell population maintenance Source: Ensembl
- histone H3-R26 methylation Source: GO_Central
- homeostasis of number of cells within a tissue Source: Ensembl
- inactivation of paternal X chromosome Source: Ensembl
- inner cell mass cell fate commitment Source: Ensembl
- negative regulation of fibroblast growth factor receptor signaling pathway Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- positive regulation of flagellated sperm motility Source: Ensembl
- positive regulation of stem cell population maintenance Source: Ensembl
- regulation of DNA methylation Source: Ensembl
- regulation of gene expression Source: GO_Central
- regulation of transcription by RNA polymerase II Source: GO_Central
- somatic stem cell population maintenance Source: Reactome
Keywordsi
Molecular function | DNA-binding, Methyltransferase, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9GZV8 |
Reactomei | R-HSA-452723, Transcriptional regulation of pluripotent stem cells |
SignaLinki | Q9GZV8 |
SIGNORi | Q9GZV8 |
Names & Taxonomyi
Protein namesi | Recommended name: PR domain zinc finger protein 14 (EC:2.1.1.-)Alternative name(s): PR domain-containing protein 14 |
Gene namesi | Name:PRDM14 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14001, PRDM14 |
MIMi | 611781, gene |
neXtProti | NX_Q9GZV8 |
VEuPathDBi | HostDB:ENSG00000147596.3 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 63978 |
OpenTargetsi | ENSG00000147596 |
PharmGKBi | PA33712 |
Miscellaneous databases
Pharosi | Q9GZV8, Tbio |
Genetic variation databases
BioMutai | PRDM14 |
DMDMi | 17368925 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047771 | 1 – 571 | PR domain zinc finger protein 14Add BLAST | 571 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 79 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9GZV8 |
jPOSTi | Q9GZV8 |
MassIVEi | Q9GZV8 |
PaxDbi | Q9GZV8 |
PeptideAtlasi | Q9GZV8 |
PRIDEi | Q9GZV8 |
ProteomicsDBi | 80159 |
PTM databases
iPTMneti | Q9GZV8 |
PhosphoSitePlusi | Q9GZV8 |
Expressioni
Tissue specificityi
Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level).2 Publications
Gene expression databases
Bgeei | ENSG00000147596, Expressed in buccal mucosa cell and 19 other tissues |
ExpressionAtlasi | Q9GZV8, baseline and differential |
Genevisiblei | Q9GZV8, HS |
Organism-specific databases
HPAi | ENSG00000147596, Tissue enhanced (testis) |
Interactioni
Subunit structurei
Interacts with CBFA2T2.
1 PublicationBinary interactionsi
Q9GZV8
Protein-protein interaction databases
BioGRIDi | 122025, 79 interactors |
DIPi | DIP-58143N |
IntActi | Q9GZV8, 79 interactors |
MINTi | Q9GZV8 |
STRINGi | 9606.ENSP00000276594 |
Miscellaneous databases
RNActi | Q9GZV8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 251 – 367 | SETPROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 194 – 384 | Interaction with CBFA2T2By similarityAdd BLAST | 191 |
Domaini
The first 5 zinc fingers, but not the last one, are required for DNA-binding and transcriptional activity.
Sequence similaritiesi
Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 400 – 424 | C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 432 – 455 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 461 – 483 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 489 – 511 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 517 – 540 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 546 – 568 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000159454 |
HOGENOMi | CLU_034551_1_0_1 |
InParanoidi | Q9GZV8 |
OMAi | WYPIPHL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9GZV8 |
TreeFami | TF106412 |
Family and domain databases
InterProi | View protein in InterPro IPR001214, SET_dom IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00317, SET, 1 hit SM00355, ZnF_C2H2, 6 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS50280, SET, 1 hit PS00028, ZINC_FINGER_C2H2_1, 5 hits PS50157, ZINC_FINGER_C2H2_2, 6 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9GZV8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALPRPSEAV PQDKVCYPPE SSPQNLAAYY TPFPSYGHYR NSLATVEEDF
60 70 80 90 100
QPFRQLEAAA SAAPAMPPFP FRMAPPLLSP GLGLQREPLY DLPWYSKLPP
110 120 130 140 150
WYPIPHVPRE VPPFLSSSHE YAGASSEDLG HQIIGGDNES GPCCGPDTLI
160 170 180 190 200
PPPPADASLL PEGLRTSQLL PCSPSKQSED GPKPSNQEGK SPARFQFTEE
210 220 230 240 250
DLHFVLYGVT PSLEHPASLH HAISGLLVPP DSSGSDSLPQ TLDKDSLQLP
260 270 280 290 300
EGLCLMQTVF GEVPHFGVFC SSFIAKGVRF GPFQGKVVNA SEVKTYGDNS
310 320 330 340 350
VMWEIFEDGH LSHFIDGKGG TGNWMSYVNC ARFPKEQNLV AVQCQGHIFY
360 370 380 390 400
ESCKEIHQNQ ELLVWYGDCY EKFLDIPVSL QVTEPGKQPS GPSEESAEGY
410 420 430 440 450
RCERCGKVFT YKYYRDKHLK YTPCVDKGDR KFPCSLCKRS FEKRDRLRIH
460 470 480 490 500
ILHVHEKHRP HKCSTCGKCF SQSSSLNKHM RVHSGDRPYQ CVYCTKRFTA
510 520 530 540 550
SSILRTHIRQ HSGEKPFKCK YCGKSFASHA AHDSHVRRSH KEDDGCSCSI
560 570
CGKIFSDQET FYSHMKFHED Y
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JMM8 | C9JMM8_HUMAN | PR domain zinc finger protein 14 | PRDM14 | 99 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021895 | 244 | K → E1 PublicationCorresponds to variant dbSNP:rs3750228Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF319458 mRNA Translation: AAG39635.1 AK022595 mRNA Translation: BAB14120.1 BC052311 mRNA Translation: AAH52311.1 |
CCDSi | CCDS6206.1 |
RefSeqi | NP_078780.1, NM_024504.3 |
Genome annotation databases
Ensembli | ENST00000276594; ENSP00000276594; ENSG00000147596 |
GeneIDi | 63978 |
KEGGi | hsa:63978 |
UCSCi | uc003xym.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF319458 mRNA Translation: AAG39635.1 AK022595 mRNA Translation: BAB14120.1 BC052311 mRNA Translation: AAH52311.1 |
CCDSi | CCDS6206.1 |
RefSeqi | NP_078780.1, NM_024504.3 |
3D structure databases
SMRi | Q9GZV8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122025, 79 interactors |
DIPi | DIP-58143N |
IntActi | Q9GZV8, 79 interactors |
MINTi | Q9GZV8 |
STRINGi | 9606.ENSP00000276594 |
PTM databases
iPTMneti | Q9GZV8 |
PhosphoSitePlusi | Q9GZV8 |
Genetic variation databases
BioMutai | PRDM14 |
DMDMi | 17368925 |
Proteomic databases
EPDi | Q9GZV8 |
jPOSTi | Q9GZV8 |
MassIVEi | Q9GZV8 |
PaxDbi | Q9GZV8 |
PeptideAtlasi | Q9GZV8 |
PRIDEi | Q9GZV8 |
ProteomicsDBi | 80159 |
Protocols and materials databases
Antibodypediai | 12206, 401 antibodies |
DNASUi | 63978 |
Genome annotation databases
Ensembli | ENST00000276594; ENSP00000276594; ENSG00000147596 |
GeneIDi | 63978 |
KEGGi | hsa:63978 |
UCSCi | uc003xym.3, human |
Organism-specific databases
CTDi | 63978 |
DisGeNETi | 63978 |
GeneCardsi | PRDM14 |
HGNCi | HGNC:14001, PRDM14 |
HPAi | ENSG00000147596, Tissue enhanced (testis) |
MIMi | 611781, gene |
neXtProti | NX_Q9GZV8 |
OpenTargetsi | ENSG00000147596 |
PharmGKBi | PA33712 |
VEuPathDBi | HostDB:ENSG00000147596.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000159454 |
HOGENOMi | CLU_034551_1_0_1 |
InParanoidi | Q9GZV8 |
OMAi | WYPIPHL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9GZV8 |
TreeFami | TF106412 |
Enzyme and pathway databases
PathwayCommonsi | Q9GZV8 |
Reactomei | R-HSA-452723, Transcriptional regulation of pluripotent stem cells |
SignaLinki | Q9GZV8 |
SIGNORi | Q9GZV8 |
Miscellaneous databases
BioGRID-ORCSi | 63978, 7 hits in 894 CRISPR screens |
GenomeRNAii | 63978 |
Pharosi | Q9GZV8, Tbio |
PROi | PR:Q9GZV8 |
RNActi | Q9GZV8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000147596, Expressed in buccal mucosa cell and 19 other tissues |
ExpressionAtlasi | Q9GZV8, baseline and differential |
Genevisiblei | Q9GZV8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR001214, SET_dom IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00317, SET, 1 hit SM00355, ZnF_C2H2, 6 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS50280, SET, 1 hit PS00028, ZINC_FINGER_C2H2_1, 5 hits PS50157, ZINC_FINGER_C2H2_2, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRD14_HUMAN | |
Accessioni | Q9GZV8Primary (citable) accession number: Q9GZV8 Secondary accession number(s): Q86UX9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with genetic variants
List of human entries with genetic variants