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Protein

WD repeat-containing protein 61

Gene

WDR61

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.4 Publications

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-429958 mRNA decay by 3' to 5' exoribonuclease
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
SignaLinkiQ9GZS3

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 61
Alternative name(s):
Meiotic recombination REC14 protein homolog
SKI8 homolog
Short name:
Ski8
Cleaved into the following chain:
Gene namesi
Name:WDR61
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000140395.8
HGNCiHGNC:30300 WDR61
MIMi609540 gene
neXtProtiNX_Q9GZS3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000140395
PharmGKBiPA142670595

Polymorphism and mutation databases

BioMutaiWDR61
DMDMi74761365

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004257481 – 305WD repeat-containing protein 61Add BLAST305
Initiator methionineiRemoved; alternateCombined sources
ChainiPRO_00002458512 – 305WD repeat-containing protein 61, N-terminally processedAdd BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei2N-acetylthreonine; in WD repeat-containing protein 61, N-terminally processedCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9GZS3
MaxQBiQ9GZS3
PaxDbiQ9GZS3
PeptideAtlasiQ9GZS3
PRIDEiQ9GZS3
ProteomicsDBi80126

PTM databases

iPTMnetiQ9GZS3
PhosphoSitePlusiQ9GZS3

Expressioni

Gene expression databases

BgeeiENSG00000140395 Expressed in 232 organ(s), highest expression level in pituitary gland
CleanExiHS_WDR61
ExpressionAtlasiQ9GZS3 baseline and differential
GenevisibleiQ9GZS3 HS

Organism-specific databases

HPAiHPA039932
HPA040065

Interactioni

Subunit structurei

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61 (PubMed:16024656, PubMed:19952111, PubMed:20178742). The PAF1 complex interacts with PHF5A. Within the PAF1 complex interacts directly with PHF5A (By similarity). Component of the SKI complex which consists of WDR61, SKIV2L and TTC37 (PubMed:16024656).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTR9Q6PD6210EBI-358545,EBI-1019583

Protein-protein interaction databases

BioGridi123255, 51 interactors
CORUMiQ9GZS3
DIPiDIP-36672N
IntActiQ9GZS3, 30 interactors
MINTiQ9GZS3
STRINGi9606.ENSP00000267973

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9GZS3
SMRiQ9GZS3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9GZS3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 57WD 1Add BLAST44
Repeati62 – 101WD 2Add BLAST40
Repeati104 – 143WD 3Add BLAST40
Repeati146 – 187WD 4Add BLAST42
Repeati188 – 227WD 5Add BLAST40
Repeati230 – 269WD 6Add BLAST40
Repeati272 – 305WD 7Add BLAST34

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4155 Eukaryota
ENOG410XP1Z LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000036765
HOVERGENiHBG059813
InParanoidiQ9GZS3
KOiK12602
OMAiERKCLHT
OrthoDBiEOG091G0F6P
PhylomeDBiQ9GZS3
TreeFamiTF324549

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 6 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q9GZS3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTNQYGILFK QEQAHDDAIW SVAWGTNKKE NSETVVTGSL DDLVKVWKWR
60 70 80 90 100
DERLDLQWSL EGHQLGVVSV DISHTLPIAA SSSLDAHIRL WDLENGKQIK
110 120 130 140 150
SIDAGPVDAW TLAFSPDSQY LATGTHVGKV NIFGVESGKK EYSLDTRGKF
160 170 180 190 200
ILSIAYSPDG KYLASGAIDG IINIFDIATG KLLHTLEGHA MPIRSLTFSP
210 220 230 240 250
DSQLLVTASD DGYIKIYDVQ HANLAGTLSG HASWVLNVAF CPDDTHFVSS
260 270 280 290 300
SSDKSVKVWD VGTRTCVHTF FDHQDQVWGV KYNGNGSKIV SVGDDQEIHI

YDCPI
Length:305
Mass (Da):33,581
Last modified:March 1, 2001 - v1
Checksum:iA26C4BBD4F8ADB80
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YN81H0YN81_HUMAN
WD repeat-containing protein 61
WDR61
294Annotation score:
H0YL19H0YL19_HUMAN
WD repeat-containing protein 61
WDR61
212Annotation score:
H0YMF9H0YMF9_HUMAN
WD repeat-containing protein 61
WDR61
189Annotation score:
H3BQA8H3BQA8_HUMAN
WD repeat-containing protein 61
WDR61
163Annotation score:
H0YLA1H0YLA1_HUMAN
WD repeat-containing protein 61
WDR61
72Annotation score:
H0YM76H0YM76_HUMAN
WD repeat-containing protein 61
WDR61
48Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91W → R in AAP97249 (Ref. 2) Curated1
Sequence conflicti176D → A in AAP97249 (Ref. 2) Curated1
Sequence conflicti229S → G in CAG33614 (Ref. 4) Curated1
Sequence conflicti255S → N in CAG33614 (Ref. 4) Curated1
Sequence conflicti259W → R in AAP97249 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309553 mRNA Translation: AAG31639.1
AF100786 mRNA Translation: AAP97225.1
AF127799 mRNA Translation: AAP97249.1
AK024754 mRNA Translation: BAB14986.1
CR457333 mRNA Translation: CAG33614.1
CH471136 Genomic DNA Translation: EAW99174.1
CH471136 Genomic DNA Translation: EAW99175.1
BC010080 mRNA Translation: AAH10080.1
CCDSiCCDS10300.1
RefSeqiNP_001290176.1, NM_001303247.1
NP_001290177.1, NM_001303248.1
NP_079510.1, NM_025234.2
XP_011520396.1, XM_011522094.1
UniGeneiHs.513055

Genome annotation databases

EnsembliENST00000267973; ENSP00000267973; ENSG00000140395
ENST00000558311; ENSP00000453801; ENSG00000140395
GeneIDi80349
KEGGihsa:80349
UCSCiuc002bdn.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309553 mRNA Translation: AAG31639.1
AF100786 mRNA Translation: AAP97225.1
AF127799 mRNA Translation: AAP97249.1
AK024754 mRNA Translation: BAB14986.1
CR457333 mRNA Translation: CAG33614.1
CH471136 Genomic DNA Translation: EAW99174.1
CH471136 Genomic DNA Translation: EAW99175.1
BC010080 mRNA Translation: AAH10080.1
CCDSiCCDS10300.1
RefSeqiNP_001290176.1, NM_001303247.1
NP_001290177.1, NM_001303248.1
NP_079510.1, NM_025234.2
XP_011520396.1, XM_011522094.1
UniGeneiHs.513055

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OW8X-ray2.30A/B/C/D2-305[»]
6GMHelectron microscopy3.10W1-305[»]
ProteinModelPortaliQ9GZS3
SMRiQ9GZS3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123255, 51 interactors
CORUMiQ9GZS3
DIPiDIP-36672N
IntActiQ9GZS3, 30 interactors
MINTiQ9GZS3
STRINGi9606.ENSP00000267973

PTM databases

iPTMnetiQ9GZS3
PhosphoSitePlusiQ9GZS3

Polymorphism and mutation databases

BioMutaiWDR61
DMDMi74761365

Proteomic databases

EPDiQ9GZS3
MaxQBiQ9GZS3
PaxDbiQ9GZS3
PeptideAtlasiQ9GZS3
PRIDEiQ9GZS3
ProteomicsDBi80126

Protocols and materials databases

DNASUi80349
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267973; ENSP00000267973; ENSG00000140395
ENST00000558311; ENSP00000453801; ENSG00000140395
GeneIDi80349
KEGGihsa:80349
UCSCiuc002bdn.4 human

Organism-specific databases

CTDi80349
EuPathDBiHostDB:ENSG00000140395.8
GeneCardsiWDR61
HGNCiHGNC:30300 WDR61
HPAiHPA039932
HPA040065
MIMi609540 gene
neXtProtiNX_Q9GZS3
OpenTargetsiENSG00000140395
PharmGKBiPA142670595
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4155 Eukaryota
ENOG410XP1Z LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000036765
HOVERGENiHBG059813
InParanoidiQ9GZS3
KOiK12602
OMAiERKCLHT
OrthoDBiEOG091G0F6P
PhylomeDBiQ9GZS3
TreeFamiTF324549

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-429958 mRNA decay by 3' to 5' exoribonuclease
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
SignaLinkiQ9GZS3

Miscellaneous databases

ChiTaRSiWDR61 human
EvolutionaryTraceiQ9GZS3
GenomeRNAii80349
PROiPR:Q9GZS3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140395 Expressed in 232 organ(s), highest expression level in pituitary gland
CleanExiHS_WDR61
ExpressionAtlasiQ9GZS3 baseline and differential
GenevisibleiQ9GZS3 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 6 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWDR61_HUMAN
AccessioniPrimary (citable) accession number: Q9GZS3
Secondary accession number(s): D3DW84, Q6IA22, Q7Z4X4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2001
Last modified: October 10, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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