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Protein

Microtubule-associated proteins 1A/1B light chain 3B

Gene

MAP1LC3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.3 Publications

Caution

PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.Curated

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagosome maturation Source: UniProtKB
  • autophagy Source: CAFA
  • autophagy of mitochondrion Source: ParkinsonsUK-UCL
  • cellular response to nitrogen starvation Source: GO_Central
  • cellular response to starvation Source: UniProtKB
  • macroautophagy Source: UniProtKB
  • positive regulation of mucus secretion Source: Ensembl

Keywordsi

Biological processAutophagy, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-1632852 Macroautophagy
R-HSA-5205685 Pink/Parkin Mediated Mitophagy
R-HSA-8854214 TBC/RABGAPs
R-HSA-8934903 Receptor Mediated Mitophagy
SIGNORiQ9GZQ8

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name:
MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene namesi
Name:MAP1LC3B
Synonyms:MAP1ALC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000140941.12
HGNCiHGNC:13352 MAP1LC3B
MIMi609604 gene
neXtProtiNX_Q9GZQ8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26Q → K: Increased interaction with WDFY3/ALFY, no effect on SQSTM1-binding; when associated with Y-27 and D-57. 1 Publication1
Mutagenesisi27H → Y: Increased interaction with WDFY3/ALFY, no effect on SQSTM1-binding; when associated with K-26 and D-57. 1 Publication1
Mutagenesisi52 – 53FL → AA: No effect on interaction with TECPR2. 1 Publication2
Mutagenesisi57H → D: Increased interaction with WDFY3/ALFY, no effect on SQSTM1-binding; when associated with K-26 and Y-27. 1 Publication1
Mutagenesisi68R → A: Impairs cleavage by ATG4B. 1 Publication1
Mutagenesisi70R → A: Abolishes interaction with TECPR2. 1 Publication1
Mutagenesisi120G → A: No processing of precursor. 1 Publication1
Mutagenesisi122K → A: No effect on processing of precursor. 1 Publication1

Organism-specific databases

DisGeNETi81631
OpenTargetsiENSG00000140941
PharmGKBiPA134923100

Polymorphism and mutation databases

BioMutaiMAP1LC3B
DMDMi17433141

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000171981 – 120Microtubule-associated proteins 1A/1B light chain 3BAdd BLAST120
PropeptideiPRO_0000017199121 – 125Removed in mature form5

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphothreonine; by PKABy similarity1
Lipidationi120Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).1 Publication
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
Phosphorylation at Thr-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120 – 121Cleavage; by ATG4BBy similarity2

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

EPDiQ9GZQ8
MaxQBiQ9GZQ8
PaxDbiQ9GZQ8
PeptideAtlasiQ9GZQ8
PRIDEiQ9GZQ8
ProteomicsDBi80115
TopDownProteomicsiQ9GZQ8

PTM databases

iPTMnetiQ9GZQ8
PhosphoSitePlusiQ9GZQ8
SwissPalmiQ9GZQ8

Miscellaneous databases

PMAP-CutDBiQ9GZQ8

Expressioni

Tissue specificityi

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.1 Publication

Gene expression databases

BgeeiENSG00000140941 Expressed in 235 organ(s), highest expression level in amniotic fluid
CleanExiHS_MAP1LC3B
ExpressionAtlasiQ9GZQ8 baseline and differential
GenevisibleiQ9GZQ8 HS

Organism-specific databases

HPAiCAB037216
HPA052474
HPA052484
HPA053767

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin (PubMed:15095872). Interacts with FYCO1 (via C-terminus) (PubMed:20100911, PubMed:24089205). Interacts with TP53INP1 and TP53INP2 (PubMed:22470510). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy (PubMed:17580304, PubMed:24089205, PubMed:24668264, PubMed:18524774). Interacts with ATG4B, MAPK15 and BNIP3 (PubMed:22948227, PubMed:23209295). Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2 (PubMed:20562859, PubMed:24089205). Interacts with TBC1D5 (PubMed:22354992). Found in a complex with UBQLN1 and UBQLN2 (PubMed:20529957). Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4 (PubMed:23459205). Interacts with ATG13 (PubMed:24290141). Interacts with RETREG2, RETREG1 and RETREG3 (PubMed:26040720). No interaction, or very weak, with WDFY3 (PubMed:24668264). Interacts with PLCL1; the interaction inhibits autophagosome formation (By similarity).By similarity16 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123565, 94 interactors
DIPiDIP-29760N
ELMiQ9GZQ8
IntActiQ9GZQ8, 381 interactors
MINTiQ9GZQ8
STRINGi9606.ENSP00000268607

Structurei

Secondary structure

1125
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9GZQ8
SMRiQ9GZQ8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9GZQ8

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiQ9GZQ8
KOiK10435
OMAiIRDQHPT
OrthoDBiEOG091G18ST
PhylomeDBiQ9GZQ8
TreeFamiTF312964

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

Q9GZQ8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE
110 120
SEKDEDGFLY MVYASQETFG MKLSV
Length:125
Mass (Da):14,688
Last modified:January 23, 2007 - v3
Checksum:iBB141DEC7653E83F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BTL1H3BTL1_HUMAN
Microtubule-associated protein 1 li...
MAP1LC3B hCG_32374
75Annotation score:
H3BTE7H3BTE7_HUMAN
Microtubule-associated proteins 1A/...
MAP1LC3B
47Annotation score:
H3BM99H3BM99_HUMAN
Microtubule-associated proteins 1A/...
MAP1LC3B
68Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61S → G in AAH67797 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303888 mRNA Translation: AAG23182.1
AF183417 mRNA Translation: AAG09686.1
AF087871 mRNA Translation: AAM10499.1
AK025556 mRNA Translation: BAB15169.1
BC018634 mRNA Translation: AAH18634.1
BC041874 mRNA Translation: AAH41874.1
BC067797 mRNA Translation: AAH67797.1
CCDSiCCDS10960.1
RefSeqiNP_073729.1, NM_022818.4
UniGeneiHs.356061

Genome annotation databases

EnsembliENST00000268607; ENSP00000268607; ENSG00000140941
GeneIDi81631
KEGGihsa:81631
UCSCiuc002fjx.4 human

Similar proteinsi

Entry informationi

Entry nameiMLP3B_HUMAN
AccessioniPrimary (citable) accession number: Q9GZQ8
Secondary accession number(s): Q6NW02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 180 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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