UniProtKB - Q9GM50 (UCHL1_HORSE)
Protein
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Gene
UCHL1
Organism
Equus caballus (Horse)
Status
Functioni
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (By similarity). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).By similarity
Miscellaneous
In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity
Caution
The homodimer may have ATP-independent ubiquitin ligase activity. However, in another study, UCHL1 was shown to lack ubiquitin ligase activity.By similarity
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 90 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 161 | Proton donorPROSITE-ProRule annotation | 1 | |
Sitei | 176 | Important for enzyme activityBy similarity | 1 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
- ligase activity Source: InterPro
- thiol-dependent ubiquitin-specific protease activity Source: GO_Central
GO - Biological processi
- protein deubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Protein family/group databases
MEROPSi | C12.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12By similarity)Short name: UCH-L1 Alternative name(s): Neuron cytoplasmic protein 9.5 PGP 9.5 Short name: PGP9.5 Ubiquitin thioesterase L1 |
Gene namesi | Name:UCHL1 |
Organismi | Equus caballus (Horse) |
Taxonomic identifieri | 9796 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Lipid-anchor By similarity
Other locations
- Cytoplasm By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211054 | 1 – 220 | Ubiquitin carboxyl-terminal hydrolase isozyme L1Add BLAST | 220 | |
PropeptideiPRO_0000414310 | 221 – 223 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 125 | PhosphoserineBy similarity | 1 | |
Lipidationi | 220 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
O-glycosylated.By similarity
Keywords - PTMi
Glycoprotein, Lipoprotein, Phosphoprotein, PrenylationProteomic databases
PaxDbi | Q9GM50 |
PeptideAtlasi | Q9GM50 |
PRIDEi | Q9GM50 |
Interactioni
Subunit structurei
Monomer. Homodimer.
Interacts with COPS5 and SNCA (By similarity).
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 5 – 10 | Interaction with ubiquitinBy similarity | 6 | |
Regioni | 211 – 216 | Interaction with ubiquitinBy similarity | 6 |
Sequence similaritiesi
Belongs to the peptidase C12 family.Curated
Phylogenomic databases
InParanoidi | Q9GM50 |
OrthoDBi | 1013351at2759 |
Family and domain databases
Gene3Di | 3.40.532.10, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001578, Peptidase_C12_UCH IPR036959, Peptidase_C12_UCH_sf IPR030297, UCHL1 |
PANTHERi | PTHR10589, PTHR10589, 1 hit PTHR10589:SF19, PTHR10589:SF19, 1 hit |
Pfami | View protein in Pfam PF01088, Peptidase_C12, 1 hit |
PRINTSi | PR00707, UBCTHYDRLASE |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00140, UCH_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9GM50-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQLKPMEINP EMLNKVLARL GVAGQWRFVD VLGLEEETLG SVPAPACALL
60 70 80 90 100
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA
110 120 130 140 150
NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDAVAQEG
160 170 180 190 200
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DLLLQDAAKV
210 220
CREFTEREQG EVRFSAVALC KAA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 187 | A → T in BAB13757 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB049188 mRNA Translation: BAB13757.1 AB013344 mRNA Translation: BAA28214.1 |
RefSeqi | NP_001075289.1, NM_001081820.1 |
Genome annotation databases
GeneIDi | 100033838 |
KEGGi | ecb:100033838 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB049188 mRNA Translation: BAB13757.1 AB013344 mRNA Translation: BAA28214.1 |
RefSeqi | NP_001075289.1, NM_001081820.1 |
3D structure databases
BMRBi | Q9GM50 |
SMRi | Q9GM50 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | C12.001 |
Proteomic databases
PaxDbi | Q9GM50 |
PeptideAtlasi | Q9GM50 |
PRIDEi | Q9GM50 |
Genome annotation databases
GeneIDi | 100033838 |
KEGGi | ecb:100033838 |
Organism-specific databases
CTDi | 7345 |
Phylogenomic databases
InParanoidi | Q9GM50 |
OrthoDBi | 1013351at2759 |
Family and domain databases
Gene3Di | 3.40.532.10, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001578, Peptidase_C12_UCH IPR036959, Peptidase_C12_UCH_sf IPR030297, UCHL1 |
PANTHERi | PTHR10589, PTHR10589, 1 hit PTHR10589:SF19, PTHR10589:SF19, 1 hit |
Pfami | View protein in Pfam PF01088, Peptidase_C12, 1 hit |
PRINTSi | PR00707, UBCTHYDRLASE |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00140, UCH_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UCHL1_HORSE | |
Accessioni | Q9GM50Primary (citable) accession number: Q9GM50 Secondary accession number(s): O62662 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | July 5, 2005 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families