UniProtKB - Q9GLG7 (CAN3_MACFA)
Protein
Calpain-3
Gene
CAPN3
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Functioni
Calcium-regulated non-lysosomal thiol-protease.
Catalytic activityi
- Broad endopeptidase activity. EC:3.4.22.54
Activity regulationi
Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 129 | PROSITE-ProRule annotation | 1 | |
Active sitei | 334 | PROSITE-ProRule annotation | 1 | |
Active sitei | 358 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 656 – 666 | 1By similarityAdd BLAST | 11 | |
Calcium bindingi | 699 – 710 | 2PROSITE-ProRule annotationBy similarityAdd BLAST | 12 | |
Calcium bindingi | 729 – 735 | 3PROSITE-ProRule annotationBy similarity | 7 | |
Calcium bindingi | 794 – 800 | 4By similarity | 7 |
GO - Molecular functioni
- calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
- calcium ion binding Source: UniProtKB
- catalytic activity Source: UniProtKB
- ligase regulator activity Source: UniProtKB
- molecular adaptor activity Source: UniProtKB
- peptidase activity Source: UniProtKB
- sodium ion binding Source: UniProtKB
- structural constituent of muscle Source: UniProtKB
- titin binding Source: UniProtKB
GO - Biological processi
- calcium-dependent self proteolysis Source: UniProtKB
- cellular response to calcium ion Source: UniProtKB
- cellular response to salt stress Source: UniProtKB
- muscle structure development Source: UniProtKB
- myofibril assembly Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of protein sumoylation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of proteolysis Source: UniProtKB
- positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
- positive regulation of satellite cell activation involved in skeletal muscle regeneration Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- protein-containing complex assembly Source: UniProtKB
- protein localization to membrane Source: UniProtKB
- proteolysis Source: UniProtKB
- regulation of catalytic activity Source: UniProtKB
- regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- response to calcium ion Source: UniProtKB
- response to muscle activity Source: UniProtKB
- sarcomere organization Source: UniProtKB
- self proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.22.54, 1793 |
Protein family/group databases
MEROPSi | C02.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Calpain-3 (EC:3.4.22.54)Alternative name(s): Calcium-activated neutral proteinase 3 Short name: CANP 3 Calpain L3 Calpain p94 Cn94 Muscle-specific calcium-activated neutral protease 3 |
Gene namesi | Name:CAPN3 |
Organismi | Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) |
Taxonomic identifieri | 9541 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca |
Proteomesi |
|
Subcellular locationi
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
- T-tubule Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- myofibril Source: UniProtKB
- protein-containing complex Source: UniProtKB
- Z disc Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207707 | 1 – 815 | Calpain-3Add BLAST | 815 |
Interactioni
Subunit structurei
Homodimer; via EF-hand domain 4.
Interacts with TTN/titin.
Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.
By similarityGO - Molecular functioni
- titin binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9541.XP_005559348.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 417 | Calpain catalyticPROSITE-ProRule annotationAdd BLAST | 344 | |
Domaini | 643 – 677 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 686 – 719 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 716 – 751 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 781 – 815 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 418 – 586 | Domain IIIAdd BLAST | 169 | |
Regioni | 587 – 649 | LinkerAdd BLAST | 63 | |
Regioni | 650 – 815 | Domain IVAdd BLAST | 166 |
Sequence similaritiesi
Belongs to the peptidase C2 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0045, Eukaryota |
GeneTreei | ENSGT00940000156092 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029531, CAPN3/9 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF329, PTHR10183:SF329, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q9GLG7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPTVISASVA PRTAAEPRSP GPVPHPAQSK ATEAGGGNAS GIYSAIISRN
60 70 80 90 100
FPIIGVKEKT FEQLHKKCLE KKVLYVDPEF PPDETSLFYS QKFPIQFIWK
110 120 130 140 150
RPPEICENPR FIIDGANRTD ICQGDLGDCW FLAAIACLTL NQRLLFRVIP
160 170 180 190 200
HDQSFIENYA GIFHFQFWRY GEWVDVVIDD CLPTYNNQLV FTKSNHRNEF
210 220 230 240 250
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVTEFFE IRDAPSDMHK
260 270 280 290 300
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLFR
310 320 330 340 350
DSDLDPRASV ERPTRTIVPV QYETRMACGL VRGHAYSVTG LDEVLFKGEK
360 370 380 390 400
VKLVRLRNPW GQVEWNGSWS DGWKDWSFVD KDEKARLQHQ VTEDGEFWMS
410 420 430 440 450
YEDFIYHFTK LEICNLTADA LQSDKLQTWT VSVNEGRWVR GCSAGGCRNF
460 470 480 490 500
PDTFWTNPQY RLKLLEEDDD PDDSEVICSF LVALMQKNRR KDRKLGANLF
510 520 530 540 550
TIGFAIYEVP KEMHGNRQHL QKDFFLYNAS RARSKTYINM REVSQRFRLP
560 570 580 590 600
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEVENTISV DRPVPIIFVS
610 620 630 640 650
DRANSNKELG VDQESEEGKG KTSPDKQEQS PQPQPGSSDQ ESEEQQQFRN
660 670 680 690 700
IFKQIAGDDM EICADELKKV LNTVVNKHKD LKTHGFTLES CRSMIALMDT
710 720 730 740 750
DGSGKLNLQE FHHLWNKIKA WQKIFKHYDT DQSGTINSYE MRNAVNDAGF
760 770 780 790 800
HLNNQLYDII TMRYADKHMN IDFDSFICCF VRLEGMFRAF HAFDKDGDGI
810
IKLNVLEWLQ LTMYA
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2K5UIF5 | A0A2K5UIF5_MACFA | Uncharacterized protein | 821 | Annotation score: | |||
A0A2K5UIG7 | A0A2K5UIG7_MACFA | Uncharacterized protein | 811 | Annotation score: | |||
A0A2K5UIG5 | A0A2K5UIG5_MACFA | Uncharacterized protein | 773 | Annotation score: | |||
A0A2K5UIH0 | A0A2K5UIH0_MACFA | Uncharacterized protein | 729 | Annotation score: | |||
A0A2K5UIJ5 | A0A2K5UIJ5_MACFA | Uncharacterized protein | 240 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF277376 mRNA Translation: AAG27599.1 |
RefSeqi | NP_001274630.1, NM_001287701.1 |
Genome annotation databases
Ensembli | ENSMFAT00000056106; ENSMFAP00000012183; ENSMFAG00000008163 |
GeneIDi | 102115794 |
KEGGi | mcf:102115794 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF277376 mRNA Translation: AAG27599.1 |
RefSeqi | NP_001274630.1, NM_001287701.1 |
3D structure databases
SMRi | Q9GLG7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9541.XP_005559348.1 |
Protein family/group databases
MEROPSi | C02.004 |
Genome annotation databases
Ensembli | ENSMFAT00000056106; ENSMFAP00000012183; ENSMFAG00000008163 |
GeneIDi | 102115794 |
KEGGi | mcf:102115794 |
Organism-specific databases
CTDi | 825 |
Phylogenomic databases
eggNOGi | KOG0045, Eukaryota |
GeneTreei | ENSGT00940000156092 |
Enzyme and pathway databases
BRENDAi | 3.4.22.54, 1793 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029531, CAPN3/9 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF329, PTHR10183:SF329, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAN3_MACFA | |
Accessioni | Q9GLG7Primary (citable) accession number: Q9GLG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2003 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries