UniProtKB - Q9GLE4 (MMP14_BOVIN)
Protein
Matrix metalloproteinase-14
Gene
MMP14
Organism
Bos taurus (Bovine)
Status
Functioni
Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 in association with pro-MMP2. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.By similarity
Catalytic activityi
- Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain. EC:3.4.24.80
Cofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 92 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 238 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 239 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 242 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 248 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metalloendopeptidase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- branching morphogenesis of an epithelial tube Source: AgBase
- cell migration Source: AgBase
- collagen catabolic process Source: GO_Central
- extracellular matrix organization Source: GO_Central
- lung development Source: AgBase
- negative regulation of Notch signaling pathway Source: UniProtKB
- positive regulation of B cell differentiation Source: UniProtKB
- positive regulation of myotube differentiation Source: UniProtKB
- skeletal system development Source: GO_Central
- zymogen activation Source: AgBase
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Calcium, Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M10.014 |
Names & Taxonomyi
Protein namesi | Recommended name: Matrix metalloproteinase-14 (EC:3.4.24.80)Short name: MMP-14 Alternative name(s): Membrane-type matrix metalloproteinase 1 Short name: MT-MMP 1 Short name: MTMMP1 Membrane-type-1 matrix metalloproteinase Short name: MT1-MMP Short name: MT1MMP |
Gene namesi | Name:MMP14 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type I membrane protein Curated
- Melanosome By similarity
- Cytoplasm By similarity
Note: Forms a complex with BST2 and localizes to the cytoplasm.By similarity
Extracellular region or secreted
- extracellular matrix Source: InterPro
Other locations
- integral component of membrane Source: UniProtKB-KW
- melanosome Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 111 – 541 | ExtracellularSequence analysisAdd BLAST | 431 | |
Transmembranei | 542 – 562 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 563 – 582 | CytoplasmicSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
PropeptideiPRO_0000289295 | 21 – 110 | Activation peptideAdd BLAST | 90 | |
ChainiPRO_0000289296 | 111 – 582 | Matrix metalloproteinase-14Add BLAST | 472 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 318 ↔ 507 | By similarity | ||
Modified residuei | 398 | Phosphotyrosine; by PKDCCBy similarity | 1 |
Post-translational modificationi
The precursor is cleaved by a furin endopeptidase.By similarity
Tyrosine phosphorylated by PKDCC/VLK.By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein, ZymogenProteomic databases
PaxDbi | Q9GLE4 |
PRIDEi | Q9GLE4 |
Interactioni
Subunit structurei
Interacts (via C-terminal cytoplasmic tail) with BST2.
Interacts with DLL1; inhibits DLL1-induced Notch signaling.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000019744 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 315 – 363 | Hemopexin 1Add BLAST | 49 | |
Repeati | 364 – 409 | Hemopexin 2Add BLAST | 46 | |
Repeati | 411 – 459 | Hemopexin 3Add BLAST | 49 | |
Repeati | 460 – 507 | Hemopexin 4Add BLAST | 48 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 90 – 97 | Cysteine switchBy similarity | 8 |
Domaini
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similaritiesi
Belongs to the peptidase M10A family.Curated
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1565, Eukaryota |
InParanoidi | Q9GLE4 |
OrthoDBi | 1075463at2759 |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021805, Pept_M10A_metallopeptidase_C IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf |
Pfami | View protein in Pfam PF11857, DUF3377, 1 hit PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9GLE4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSPAPRPACS LLLPVLTLAS ALASLSSAQS SFSPEAWLQQ HGYLPPGDLR
60 70 80 90 100
THTQRSPQSL SAAIAAMQRF YGLRVTGKAD ADTMKAMRRP RCGVPDKFGA
110 120 130 140 150
EIKANVRRKR YAIQGLKWQH NEITFCIQNY TPNVGEYATF EAIRKAFRVW
160 170 180 190 200
ESATPLRFRE VPYAYIREGH EKQADIMIFF AEGFHGDSTP FDGEGGFLAH
210 220 230 240 250
AYFPGPNIGG DTHFDSAEPW TVRNEDLNGN DIFLVAVHEL GHALGLEHSN
260 270 280 290 300
DPSAIMAPFY QWMDTENFVL PDDDRRGIQQ LYGSKSGSPT KMPPQPRTTS
310 320 330 340 350
RPSVPDKPKN PTYGPNICDG NFDTVAMLRG EMFVFKERWF WRVRKNQVMD
360 370 380 390 400
GYPMPIGQFW RGLPASINTA YERKDGKFVF FKGDKHWVFD EASLEPGYPK
410 420 430 440 450
HIKELGRGLP TDRIDAALFW MPNGKTYFFR GNKYYRFNEE LRIVESEYPK
460 470 480 490 500
NIKVWEGIPE SPRGSFMGSD EVFTYFYKGN KYWKFNNQKL KVEPGYPKSA
510 520 530 540 550
LRDWMGCPSS GGQPDEGTEE ETEVIIIEVD EEGSGAVSAA AVVLPVLLLL
560 570 580
LVLAVGLAVF FFRRHGTPKR LLYCQRSLLD KV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF290429 mRNA Translation: AAG28170.1 |
RefSeqi | NP_776815.1, NM_174390.2 |
Genome annotation databases
GeneIDi | 281915 |
KEGGi | bta:281915 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF290429 mRNA Translation: AAG28170.1 |
RefSeqi | NP_776815.1, NM_174390.2 |
3D structure databases
SMRi | Q9GLE4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000019744 |
Protein family/group databases
MEROPSi | M10.014 |
Proteomic databases
PaxDbi | Q9GLE4 |
PRIDEi | Q9GLE4 |
Genome annotation databases
GeneIDi | 281915 |
KEGGi | bta:281915 |
Organism-specific databases
CTDi | 4323 |
Phylogenomic databases
eggNOGi | KOG1565, Eukaryota |
InParanoidi | Q9GLE4 |
OrthoDBi | 1075463at2759 |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021805, Pept_M10A_metallopeptidase_C IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf |
Pfami | View protein in Pfam PF11857, DUF3377, 1 hit PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMP14_BOVIN | |
Accessioni | Q9GLE4Primary (citable) accession number: Q9GLE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries