UniProtKB - Q9GKX2 (DHRS4_RABIT)
Dehydrogenase/reductase SDR family member 4
DHRS4
Functioni
NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones (PubMed:12604222, PubMed:9880795, PubMed:19056333).
Reduces all-trans-retinal and 9-cis retinal (Probable). Reduces 3-ketosteroids and benzil into 3alpha-hydroxysteroids and S-benzoin, respectively, in contrast to the stereoselectivity of primates DHRS4s which produce 3beta-hydroxysteroids and R-benzoin (By similarity).
In the reverse reaction, catalyze the NADP-dependent oxidation of 3alpha-hydroxysteroids and alcohol, but with much lower efficiency (By similarity).
Involved in the metabolism of 3alpha-hydroxysteroids, retinoid, isatin and xenobiotic carbonyl compounds (PubMed:12604222, PubMed:19056333).
By similarity1 Publication3 PublicationsMiscellaneous
Catalytic activityi
- EC:1.1.1.1843 PublicationsThis reaction proceeds in the backward3 Publications direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- EC:1.1.1.3001 PublicationThis reaction proceeds in the backward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Activity regulationi
Kineticsi
- KM=0.028 mM for 16-Ketoestrone (at pH6)1 Publication
- KM=0.045 mM for 16-Ketoestrone (at pH7.4)1 Publication
- KM=0.007 mM for all-trans-Retinal (at pH7.4)1 Publication
- KM=0.079 mM for 9-cis-retinal (at pH7.4)1 Publication
- KM=0.11 mM for Isatin (at pH6)1 Publication
- KM=0.061 mM for Isatin (at pH7.4)1 Publication
- KM=0.89 mM for Propiophenone (at pH6)1 Publication
- KM=0.063 mM for Heptanophenone (at pH6)1 Publication
- KM=0.027 mM for Nonanophenone (at pH6)1 Publication
- KM=0.095 mM for 4-Hexanoylpyridine (at pH6)1 Publication
- KM=0.022 mM for Hexanophenone (at pH6)1 Publication
- KM=0.11 mM for Valerophenone (at pH6)1 Publication
- KM=0.18 mM for n-Butyrophenone (at pH6)1 Publication
- KM=0.52 mM for 3-Benzoylpyridine (at pH6)1 Publication
- KM=0.69 mM for 4-Benzoylpyridine (at pH6)1 Publication
- KM=1.7 mM for 2,3-Hexanedione (at pH6)1 Publication
- KM=0.4 mM for 1-phenyl-1,2-propanedione (at pH6)1 Publication
- KM=0.11 mM for Benzil (at pH6)1 Publication
- KM=13 mM for Diacetyl (at pH6)1 Publication
- KM=1.8 mM for Pyridine-4-aldehyde (at pH6)1 Publication
- KM=0.006 mM for 1-Phenylisatin (at pH6)1 Publication
- KM=0.001 mM for 9,10-Phenanthrenequinone (at pH6)1 Publication
- KM=0.001 mM for 9,10-Phenanthrenequinone (at pH7.4)1 Publication
- KM=0.12 mM for Menadione (at pH6)1 Publication
- KM=0.27 mM for S---1-Phenyl-1-butanol (at pH7.4)1 Publication
- KM=0.034 mM for all-trans-Retinol (at pH7.4)1 Publication
- KM=0.33 mM for 3-Benzoylpyridine (at pH6)1 Publication
- KM=0.63 mM for 4-Benzoylpyridine (at pH6)1 Publication
- KM=1.55 mM for 4-Acetylpyridine (at pH6)1 Publication
- KM=1.8 mM for Pyridine-3-aldehyde (at pH6)1 Publication
- KM=0.9 mM for Pyridine-4-aldehyde (at pH6)1 Publication
- KM=0.59 mM for 4-Nitroacetophenone (at pH6)1 Publication
- KM=0.18 mM for Menadione (at pH6)1 Publication
- KM=0.028 mM for 5beta-Pregnane-3,20-dione (at pH7.4)1 Publication
- Vmax=48.7 µmol/min/mg enzyme with 16-Ketoestrone as substrate (at pH6)1 Publication
- Vmax=24.2 µmol/min/mg enzyme with 16-Ketoestrone as substrate (at pH7.4)1 Publication
- Vmax=2.54 µmol/min/mg enzyme with all-trans-Retinal as substrate (at pH7.4)1 Publication
- Vmax=6.34 µmol/min/mg enzyme with 9-cis-retinal as substrate (at pH7.4)1 Publication
- Vmax=83.6 µmol/min/mg enzyme with Isatin as substrate (at pH6)1 Publication
- Vmax=34.5 µmol/min/mg enzyme with Isatin as substrate (at pH7.4)1 Publication
- Vmax=1 µmol/min/mg enzyme with Propiophenone as substrate (at pH6)1 Publication
- Vmax=6.5 µmol/min/mg enzyme with Heptanophenone as substrate (at pH6)1 Publication
- Vmax=3.5 µmol/min/mg enzyme with Nonanophenoneas substrate (at pH6)1 Publication
- Vmax=55.7 µmol/min/mg enzyme with 4-Hexanoylpyridine as substrate (at pH6)1 Publication
- Vmax=5.8 µmol/min/mg enzyme with Hexanophenone as substrate (at pH6)1 Publication
- Vmax=5.8 µmol/min/mg enzyme with Valerophenone as substrate (at pH6)1 Publication
- Vmax=3.3 µmol/min/mg enzyme with n-Butyrophenone as substrate (at pH6)1 Publication
- Vmax=3.8 µmol/min/mg enzyme with 3-benzoylpyridine as substrate (at pH6)1 Publication
- Vmax=13.3 µmol/min/mg enzyme with 4-Benzoylpyridine as substrate (at pH6)1 Publication
- Vmax=115 µmol/min/mg enzyme with 2,3-Hexanedione as substrate (at pH6)1 Publication
- Vmax=115 µmol/min/mg enzyme with 1-phenyl-1,2-propanedione as substrate (at pH6)1 Publication
- Vmax=66.8 µmol/min/mg enzyme for the reduction of Benzil into S-benzoin (at pH6)1 Publication
- Vmax=110 µmol/min/mg enzyme with Diacetyl as substrate (at pH6)1 Publication
- Vmax=98.9 µmol/min/mg enzyme with Pyridine-4-aldehyde as substrate (at pH6)1 Publication
- Vmax=152 µmol/min/mg enzyme with 1-Phenylisatin as substrate (at pH6)1 Publication
- Vmax=121 µmol/min/mg enzyme with 9,10-Phenanthrenequinone as substrate (at pH6)1 Publication
- Vmax=78 µmol/min/mg enzyme with 9,10-Phenanthrenequinone as substrate (at pH7.4)1 Publication
- Vmax=2.6 µmol/min/mg enzyme with Menadione as substrate (at pH6)1 Publication
- Vmax=1.35 µmol/min/mg enzyme with S---1-Phenyl-1-butanol as substrate (at pH7.4)1 Publication
- Vmax=0.034 µmol/min/mg enzyme with all-trans-Retinol as substrate (at pH7.4)1 Publication
- Vmax=0.76 µmol/min/mg enzyme with 3-Benzoylpyridine as substrate (at pH6)1 Publication
- Vmax=2.98 µmol/min/mg enzyme with 4-Benzoylpyridine as substrate (at pH6)1 Publication
- Vmax=1.76 µmol/min/mg enzyme with 4-Acetylpyridine as substrate (at pH6)1 Publication
- Vmax=3.64 µmol/min/mg enzyme with Pyridine-3-aldehyde as substrate (at pH6)1 Publication
- Vmax=10.5 µmol/min/mg enzyme with Pyridine-4-aldehyde as substrate (at pH6)1 Publication
- Vmax=1.73 µmol/min/mg enzyme with 4-Nitroacetophenone as substrate (at pH6)1 Publication
- Vmax=2.47 µmol/min/mg enzyme with Menadione as substrate (at pH6)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 151 | SubstrateBy similarity | 1 | |
Sitei | 158 | Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoinBy similarity | 1 | |
Sitei | 161 | Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoinBy similarity | 1 | |
Active sitei | 164 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 168 | NADPBy similarity | 1 | |
Sitei | 177 | Important for the maintenance of the quaternary structure, the catalytic activity and cold stabilityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 18 – 42 | NADPBy similarityAdd BLAST | 25 |
GO - Molecular functioni
- carbonyl reductase (NADPH) activity Source: UniProtKB
- identical protein binding Source: UniProtKB
- NADP-retinol dehydrogenase activity Source: UniProtKB
GO - Biological processi
- cellular ketone metabolic process Source: UniProtKB
- steroid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.300, 1749 |
Names & Taxonomyi
Protein namesi | Recommended name: Dehydrogenase/reductase SDR family member 41 Publication (EC:1.1.1.1843 Publications, EC:1.1.1.3001 Publication)Alternative name(s): NADPH-dependent carbonyl reductase1 Publication Short name: CR1 Publication Short name: RACR1 Publication NADPH-dependent retinol dehydrogenase/reductase1 Publication Short name: NDRD1 Publication Peroxisomal short-chain alcohol dehydrogenase Short name: PSCD Short chain dehydrogenase/reductase family 25C member 2By similarity Short name: Protein SDR25C2By similarity rabNRDR |
Gene namesi | Name:DHRS4 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Peroxisome
- peroxisome Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054651 | ‹1 – 260 | Dehydrogenase/reductase SDR family member 4Add BLAST | ›260 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 74 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 74 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 198 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 198 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 202 | PhosphoserineBy similarity | 1 | |
Modified residuei | 209 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Homotetramer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000019647 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 258 – 260 | Peroxisomal targeting signalBy similarity | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
InParanoidi | Q9GKX2 |
Family and domain databases
InterProi | View protein in InterPro IPR029511, DHRS4-like IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PANTHERi | PTHR43943:SF8, PTHR43943:SF8, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Fragment.
10 20 30 40 50
MASSGVTRRD PLANKVAIVT ASTDGIGLAI ARRLAQDGAH VVISSRKQQN
60 70 80 90 100
VDRAVAALQA EGLSVTGTVC HVGKAEDRER LVATALNLHG GIDILVSNAA
110 120 130 140 150
VNPFFGKLMD VTEEVWDKIL DINVKAMALM TKAVVPEMEK RGGGSVVIVA
160 170 180 190 200
SIAAFNPFSG LGPYNVSKTA LVGLTKNLAL ELAAQNIRVN CLAPGLIKTS
210 220 230 240 250
FSKALWEDKA QEENIIQKLR IRRLGKPEEC AGIVSFLCSE DASYITGETV
260
VVAGGAPSRL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB045133 mRNA Translation: BAB18777.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB045133 mRNA Translation: BAB18777.1 |
3D structure databases
AlphaFoldDBi | Q9GKX2 |
SMRi | Q9GKX2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000019647 |
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
InParanoidi | Q9GKX2 |
Enzyme and pathway databases
BRENDAi | 1.1.1.300, 1749 |
Family and domain databases
InterProi | View protein in InterPro IPR029511, DHRS4-like IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PANTHERi | PTHR43943:SF8, PTHR43943:SF8, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHRS4_RABIT | |
Accessioni | Q9GKX2Primary (citable) accession number: Q9GKX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families