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UniProtKB - Q9GJX5 (CP4AL_PIG)
Protein
Taurochenodeoxycholic 6 alpha-hydroxylase
Gene
CYP4A21
Organism
Sus scrofa (Pig)
Status
Functioni
Catalyzes the 6 alpha hydroxylation oxidation of taurodeoxycholate to produce the pig specific bile acid taurohyocholic acid.
3 PublicationsCatalytic activityi
- O2 + reduced [NADPH—hemoprotein reductase] + taurochenodeoxycholate = H+ + H2O + oxidized [NADPH—hemoprotein reductase] + taurohyocholate2 PublicationsEC:1.14.14.572 Publications
- lithocholate + O2 + reduced [NADPH—hemoprotein reductase] = H+ + H2O + hyodeoxycholate + oxidized [NADPH—hemoprotein reductase]2 PublicationsEC:1.14.14.572 Publications
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 451 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- taurochenodeoxycholate 6alpha-hydroxylase activity Source: RHEA
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
BRENDAi | 1.14.14.57, 6170 |
Names & Taxonomyi
Protein namesi | Recommended name: Taurochenodeoxycholic 6 alpha-hydroxylase (EC:1.14.14.572 Publications)Alternative name(s): CYPIVA21 Cytochrome P450 4A21 |
Gene namesi | Name:CYP4A21 |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 6 – 26 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 110 – 130 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 314 – 319 | AAGHDS → FEGHDT: Complete loss of activity. 1 Publication | 6 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000280742 | 2 – 504 | Taurochenodeoxycholic 6 alpha-hydroxylaseAdd BLAST | 503 |
Proteomic databases
PaxDbi | Q9GJX5 |
PeptideAtlasi | Q9GJX5 |
Expressioni
Tissue specificityi
Primarily expressed in liver. Low expression in kidney.1 Publication
Gene expression databases
ExpressionAtlasi | Q9GJX5, baseline |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000155173 |
InParanoidi | Q9GJX5 |
OrthoDBi | 1247045at2759 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9GJX5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVPALASVS GLLQVASLLG LLLLLLKAAQ LYLRRQWLLK ALQQFPSPPS
60 70 80 90 100
HWLYGHSREF QEESELQPLL KRVEKYPSAC ARWLWGTRAM VLVYDPDYMK
110 120 130 140 150
VVLARSEPKA PVLYRLLIPW IGCGLLLLNG QMWFQRRRML TPAFHYDILK
160 170 180 190 200
PYVGLMAKSV QVMLDKWEQL VAQDPRLEIV GPVSLMTLDT IMKCAFSHQG
210 220 230 240 250
SAQTDGDSQS YIQAIWDLKN LIFSRLRSAF LQNDIIYRLS PEGRQCQRAC
260 270 280 290 300
QKVHQHTDRV IQLRKTHLQK EGEMENVKKK RHLDFLDILL FARMENGNSL
310 320 330 340 350
SDTDVRAEVD TFMAAGHDST ASGISWVLYA LASNPEHQQR CREEIQGLLG
360 370 380 390 400
DGTSITWDHL DQMPYTTMCI KEALRLYPPV PSVGRELSKP ITFPDGRSLP
410 420 430 440 450
AGIILSLSIY GLHHNPQVWP NPEEFDPSRF APGSARHSHA FMPFSGGSRN
460 470 480 490 500
CIGKQFAMNE MKVVVALTLL RFELAPDPSR IPVPIQGIVL KSKNGIHLNL
RKIP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 135 | Q → P in CAE52532 (PubMed:14641109).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278474 mRNA Translation: CAC19358.1 AJ586859 Genomic DNA Translation: CAE52546.1 AJ586618 Genomic DNA Translation: CAE52532.1 |
RefSeqi | NP_999590.1, NM_214425.1 |
Genome annotation databases
Ensembli | ENSSSCT00000004307; ENSSSCP00000004207; ENSSSCG00000003891 |
GeneIDi | 403327 |
KEGGi | ssc:403327 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278474 mRNA Translation: CAC19358.1 AJ586859 Genomic DNA Translation: CAE52546.1 AJ586618 Genomic DNA Translation: CAE52532.1 |
RefSeqi | NP_999590.1, NM_214425.1 |
3D structure databases
SMRi | Q9GJX5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000004207 |
Proteomic databases
PaxDbi | Q9GJX5 |
PeptideAtlasi | Q9GJX5 |
Genome annotation databases
Ensembli | ENSSSCT00000004307; ENSSSCP00000004207; ENSSSCG00000003891 |
GeneIDi | 403327 |
KEGGi | ssc:403327 |
Organism-specific databases
CTDi | 403327 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000155173 |
InParanoidi | Q9GJX5 |
OrthoDBi | 1247045at2759 |
Enzyme and pathway databases
BRENDAi | 1.14.14.57, 6170 |
Gene expression databases
ExpressionAtlasi | Q9GJX5, baseline |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP4AL_PIG | |
Accessioni | Q9GJX5Primary (citable) accession number: Q9GJX5 Secondary accession number(s): Q70BW2, Q70BZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | June 2, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families