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UniProtKB - Q9FV49 (MAP21_ARATH)
Protein
Methionine aminopeptidase 2A
Gene
MAP2A
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).
UniRule annotationCatalytic activityi
- Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation EC:3.4.11.18
Cofactori
Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 194 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 214 | Divalent metal cation 1UniRule annotation | 1 | |
Metal bindingi | 225 | Divalent metal cation 1UniRule annotation | 1 | |
Metal bindingi | 225 | Divalent metal cation 2; catalyticUniRule annotation | 1 | |
Metal bindingi | 294 | Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation | 1 | |
Binding sitei | 302 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 327 | Divalent metal cation 2; catalyticUniRule annotation | 1 | |
Metal bindingi | 422 | Divalent metal cation 1UniRule annotation | 1 | |
Metal bindingi | 422 | Divalent metal cation 2; catalyticUniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-UniRule
- metalloaminopeptidase activity Source: UniProtKB-UniRule
GO - Biological processi
- N-terminal protein amino acid modification Source: TAIR
- protein initiator methionine removal Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Protease |
Ligand | Metal-binding |
Protein family/group databases
MEROPSi | M24.A02 |
Names & Taxonomyi
Protein namesi | Recommended name: Methionine aminopeptidase 2AUniRule annotation (EC:3.4.11.18UniRule annotation)Short name: MAP 2AUniRule annotation Short name: MetAP 2AUniRule annotation Alternative name(s): Peptidase MUniRule annotation |
Gene namesi | Name:MAP2AUniRule annotation Ordered Locus Names:At2g44180 ORF Names:F6E13.31 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G44180 |
TAIRi | locus:2051849, AT2G44180 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Other locations
- cytoplasm Source: TAIR
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000148970 | 1 – 441 | Methionine aminopeptidase 2AAdd BLAST | 441 |
Proteomic databases
PaxDbi | Q9FV49 |
PRIDEi | Q9FV49 |
ProteomicsDBi | 238868 |
PTM databases
iPTMneti | Q9FV49 |
Expressioni
Tissue specificityi
Ubiquitous. Preferentially expressed in roots.1 Publication
Gene expression databases
ExpressionAtlasi | Q9FV49, baseline and differential |
Genevisiblei | Q9FV49, AT |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 103 | DisorderedSequence analysisAdd BLAST | 103 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 34 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 36 – 61 | Basic and acidic residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | KOG2775, Eukaryota |
HOGENOMi | CLU_015857_7_1_1 |
InParanoidi | Q9FV49 |
OrthoDBi | 601484at2759 |
PhylomeDBi | Q9FV49 |
Family and domain databases
CDDi | cd01088, MetAP2, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.90.230.10, 1 hit |
HAMAPi | MF_03175, MetAP_2_euk, 1 hit |
InterProi | View protein in InterPro IPR036005, Creatinase/aminopeptidase-like IPR000994, Pept_M24 IPR001714, Pept_M24_MAP IPR002468, Pept_M24A_MAP2 IPR018349, Pept_M24A_MAP2_BS IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00557, Peptidase_M24, 1 hit |
PRINTSi | PR00599, MAPEPTIDASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF55920, SSF55920, 1 hit |
TIGRFAMsi | TIGR00501, met_pdase_II, 1 hit |
PROSITEi | View protein in PROSITE PS01202, MAP_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9FV49-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIGNPEVAT MGKENTEAES SNGNESQLSS DLTKSLDLAE VKEDEKDNNQ
60 70 80 90 100
EEEDGLKAEA STKKKKKKSK SKKKKSSLQQ TDPPSIPVLE LFPSGDFPQG
110 120 130 140 150
EIQQYNDDNL WRTTSEEKRE MERLQKPIYN SLRQAAEVHR QVRKYMRSIL
160 170 180 190 200
KPGMLMIDLC ETLENTVRKL ISENGLQAGI AFPTGCSLNN VAAHWTPNSG
210 220 230 240 250
DKTVLQYDDV MKLDFGTHID GHIVDSAFTV AFNPMFDPLL AASRDATYTG
260 270 280 290 300
IKEAGVDVRL CDVGAAVQEV MESYEVEING KVYQVKSIRN LNGHSIGRYQ
310 320 330 340 350
IHAEKSVPNV RGGEQTKMEE GELYAIETFG STGKGYVRED LECSHYMKNY
360 370 380 390 400
DVGHVPLRLP RAKQLLATIN KNFSTLAFCR RYLDRLGETK YLMALKNLCD
410 420 430 440
SGIIEPCPPV CDVKGSYISQ FEHTILLRPT CKEIISKGDD Y
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A178VUM7 | A0A178VUM7_ARATH | Methionine aminopeptidase 2 | MAP2A methionine aminopeptidase 2A, At2g44180, F6E13.31 | 321 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | G → E in AAG33978 (PubMed:11060042).Curated | 1 | |
Sequence conflicti | 51 | Missing in AAG33978 (PubMed:11060042).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF250964 mRNA Translation: AAG33978.1 AC004005 Genomic DNA Translation: AAC23422.2 CP002685 Genomic DNA Translation: AEC10386.1 BT006476 mRNA Translation: AAP21284.1 |
PIRi | T00698 |
RefSeqi | NP_566013.1, NM_129981.5 |
Genome annotation databases
EnsemblPlantsi | AT2G44180.1; AT2G44180.1; AT2G44180 |
GeneIDi | 819025 |
Gramenei | AT2G44180.1; AT2G44180.1; AT2G44180 |
KEGGi | ath:AT2G44180 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF250964 mRNA Translation: AAG33978.1 AC004005 Genomic DNA Translation: AAC23422.2 CP002685 Genomic DNA Translation: AEC10386.1 BT006476 mRNA Translation: AAP21284.1 |
PIRi | T00698 |
RefSeqi | NP_566013.1, NM_129981.5 |
3D structure databases
SMRi | Q9FV49 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G44180.1 |
Protein family/group databases
MEROPSi | M24.A02 |
PTM databases
iPTMneti | Q9FV49 |
Proteomic databases
PaxDbi | Q9FV49 |
PRIDEi | Q9FV49 |
ProteomicsDBi | 238868 |
Genome annotation databases
EnsemblPlantsi | AT2G44180.1; AT2G44180.1; AT2G44180 |
GeneIDi | 819025 |
Gramenei | AT2G44180.1; AT2G44180.1; AT2G44180 |
KEGGi | ath:AT2G44180 |
Organism-specific databases
Araporti | AT2G44180 |
TAIRi | locus:2051849, AT2G44180 |
Phylogenomic databases
eggNOGi | KOG2775, Eukaryota |
HOGENOMi | CLU_015857_7_1_1 |
InParanoidi | Q9FV49 |
OrthoDBi | 601484at2759 |
PhylomeDBi | Q9FV49 |
Miscellaneous databases
PROi | PR:Q9FV49 |
Gene expression databases
ExpressionAtlasi | Q9FV49, baseline and differential |
Genevisiblei | Q9FV49, AT |
Family and domain databases
CDDi | cd01088, MetAP2, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.90.230.10, 1 hit |
HAMAPi | MF_03175, MetAP_2_euk, 1 hit |
InterProi | View protein in InterPro IPR036005, Creatinase/aminopeptidase-like IPR000994, Pept_M24 IPR001714, Pept_M24_MAP IPR002468, Pept_M24A_MAP2 IPR018349, Pept_M24A_MAP2_BS IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00557, Peptidase_M24, 1 hit |
PRINTSi | PR00599, MAPEPTIDASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF55920, SSF55920, 1 hit |
TIGRFAMsi | TIGR00501, met_pdase_II, 1 hit |
PROSITEi | View protein in PROSITE PS01202, MAP_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAP21_ARATH | |
Accessioni | Q9FV49Primary (citable) accession number: Q9FV49 Secondary accession number(s): O80587 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2006 |
Last sequence update: | January 24, 2006 | |
Last modified: | February 23, 2022 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families