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UniProtKB - Q9FUJ3 (CKX2_ARATH)
Protein
Cytokinin dehydrogenase 2
Gene
CKX2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group.
1 PublicationCatalytic activityi
- EC:1.5.99.121 Publication
Cofactori
FADBy similarity
Kineticsi
- KM=0.3 µM for isopentenyladenine1 Publication
- Vmax=7.4 nmol/h/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 97 | FAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 150 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 155 | FADBy similarity | 1 | |
Binding sitei | 216 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 460 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 87 – 91 | FADBy similarity | 5 | |
Nucleotide bindingi | 92 – 93 | FADBy similarity | 2 | |
Nucleotide bindingi | 161 – 165 | FADBy similarity | 5 | |
Nucleotide bindingi | 495 – 498 | FADBy similarity | 4 |
GO - Molecular functioni
- cytokinin dehydrogenase activity Source: TAIR
- FAD binding Source: InterPro
- oxidoreductase activity Source: GO_Central
- primary amine oxidase activity Source: TAIR
GO - Biological processi
- cytokinin catabolic process Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | ARA:AT2G19500-MONOMER |
BRENDAi | 1.5.99.12, 399 |
SABIO-RKi | Q9FUJ3 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytokinin dehydrogenase 2 (EC:1.5.99.121 Publication)Alternative name(s): Cytokinin oxidase 2 Short name: AtCKX2 Short name: CKO 2 |
Gene namesi | Name:CKX2 Ordered Locus Names:At2g19500 ORF Names:F3P11.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G19500 |
TAIRi | locus:2050349, AT2G19500 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Extracellular region or secreted
- extracellular space 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: TAIR
Extracellular region or secreted
- extracellular space Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulum, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000020419 | 23 – 501 | Cytokinin dehydrogenase 2Add BLAST | 479 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 32 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 51 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 92 | Pros-8alpha-FAD histidineBy similarity | 1 | |
Glycosylationi | 107 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9FUJ3 |
ProteomicsDBi | 222613 |
Expressioni
Tissue specificityi
Expressed in the shoot apex, in stipules, and occasionally in the most apical part of the inflorescence stems. Not detected in roots.1 Publication
Gene expression databases
ExpressionAtlasi | Q9FUJ3, baseline and differential |
Genevisiblei | Q9FUJ3, AT |
Interactioni
Protein-protein interaction databases
STRINGi | 3702.AT2G19500.1 |
Chemistry databases
BindingDBi | Q9FUJ3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 53 – 226 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 174 |
Sequence similaritiesi
Belongs to the oxygen-dependent FAD-linked oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q9FUJ3 |
OMAi | RAKWFRM |
OrthoDBi | 440623at2759 |
PhylomeDBi | Q9FUJ3 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9FUJ3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANLRLMITL ITVLMITKSS NGIKIDLPKS LNLTLSTDPS IISAASHDFG
60 70 80 90 100
NITTVTPGGV ICPSSTADIS RLLQYAANGK STFQVAARGQ GHSLNGQASV
110 120 130 140 150
SGGVIVNMTC ITDVVVSKDK KYADVAAGTL WVDVLKKTAE KGVSPVSWTD
160 170 180 190 200
YLHITVGGTL SNGGIGGQVF RNGPLVSNVL ELDVITGKGE MLTCSRQLNP
210 220 230 240 250
ELFYGVLGGL GQFGIITRAR IVLDHAPKRA KWFRMLYSDF TTFTKDQERL
260 270 280 290 300
ISMANDIGVD YLEGQIFLSN GVVDTSFFPP SDQSKVADLV KQHGIIYVLE
310 320 330 340 350
VAKYYDDPNL PIISKVIDTL TKTLSYLPGF ISMHDVAYFD FLNRVHVEEN
360 370 380 390 400
KLRSLGLWEL PHPWLNLYVP KSRILDFHNG VVKDILLKQK SASGLALLYP
410 420 430 440 450
TNRNKWDNRM SAMIPEIDED VIYIIGLLQS ATPKDLPEVE SVNEKIIRFC
460 470 480 490 500
KDSGIKIKQY LMHYTSKEDW IEHFGSKWDD FSKRKDLFDP KKLLSPGQDI
F
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 157 | G → R in AAG30905 (PubMed:11154345).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF303978 mRNA Translation: AAG30905.1 AC005917 Genomic DNA Translation: AAD10149.2 CP002685 Genomic DNA Translation: AEC06889.1 BT004107 mRNA Translation: AAO42130.1 BT005653 mRNA Translation: AAO64073.1 |
PIRi | E84577 |
RefSeqi | NP_565455.1, NM_127508.2 |
Genome annotation databases
EnsemblPlantsi | AT2G19500.1; AT2G19500.1; AT2G19500 |
GeneIDi | 816469 |
Gramenei | AT2G19500.1; AT2G19500.1; AT2G19500 |
KEGGi | ath:AT2G19500 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF303978 mRNA Translation: AAG30905.1 AC005917 Genomic DNA Translation: AAD10149.2 CP002685 Genomic DNA Translation: AEC06889.1 BT004107 mRNA Translation: AAO42130.1 BT005653 mRNA Translation: AAO64073.1 |
PIRi | E84577 |
RefSeqi | NP_565455.1, NM_127508.2 |
3D structure databases
SMRi | Q9FUJ3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G19500.1 |
Chemistry databases
BindingDBi | Q9FUJ3 |
ChEMBLi | CHEMBL6133 |
Proteomic databases
PaxDbi | Q9FUJ3 |
ProteomicsDBi | 222613 |
Genome annotation databases
EnsemblPlantsi | AT2G19500.1; AT2G19500.1; AT2G19500 |
GeneIDi | 816469 |
Gramenei | AT2G19500.1; AT2G19500.1; AT2G19500 |
KEGGi | ath:AT2G19500 |
Organism-specific databases
Araporti | AT2G19500 |
TAIRi | locus:2050349, AT2G19500 |
Phylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q9FUJ3 |
OMAi | RAKWFRM |
OrthoDBi | 440623at2759 |
PhylomeDBi | Q9FUJ3 |
Enzyme and pathway databases
BioCyci | ARA:AT2G19500-MONOMER |
BRENDAi | 1.5.99.12, 399 |
SABIO-RKi | Q9FUJ3 |
Miscellaneous databases
PROi | PR:Q9FUJ3 |
Gene expression databases
ExpressionAtlasi | Q9FUJ3, baseline and differential |
Genevisiblei | Q9FUJ3, AT |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CKX2_ARATH | |
Accessioni | Q9FUJ3Primary (citable) accession number: Q9FUJ3 Secondary accession number(s): Q9ZUP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2002 |
Last sequence update: | August 2, 2002 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families