UniProtKB - Q9FMZ0 (BKI1_ARATH)
Protein
BRI1 kinase inhibitor 1
Gene
BKI1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Negative regulator of brassinosteroid signaling. When associated to the membrane, limits the interaction of BRI1 with BAK1 by binding to the kinase-inactive form of BRI1.1 Publication
Miscellaneous
BRI1 is not required for BKI1 association with the plasma membrane, but the BRI1 kinase activity is necessary for the membrane release.
GO - Molecular functioni
- protein heterodimerization activity Source: TAIR
- protein kinase inhibitor activity Source: TAIR
GO - Biological processi
- brassinosteroid mediated signaling pathway Source: UniProtKB-KW
- lipid metabolic process Source: UniProtKB-KW
- negative regulation of brassinosteroid biosynthetic process Source: TAIR
Keywordsi
Biological process | Brassinosteroid signaling pathway, Lipid metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: BRI1 kinase inhibitor 1 |
Gene namesi | Name:BKI1 Ordered Locus Names:At5g42750 ORF Names:MJB21.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G42750 |
TAIRi | locus:2165472, AT5G42750 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Other locations
- Cytoplasm 1 Publication
Note: Once phosphorylated following brassinosteroid treatment, displaced from the plasma membrane into the cytosol where it is inactive.
Cytosol
- cytosol Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 179 – 180 | RK → AA: No effect on subcellular location. Cytosolic only; when associated with 197-A-A-198 or 197-A-A-198 and 209-A-A-210 and 218-A-A-219. 1 Publication | 2 | |
Mutagenesisi | 197 – 198 | KK → AA: Reduced cell membrane localization. Cytosolic only; when associated with 179-A-A-180 or 179-A-A-180 and 209-A-A-210 and 218-A-A-219. 1 Publication | 2 | |
Mutagenesisi | 209 – 210 | KK → AA: Reduced cell membrane localization. Cytosolic only; when associated with 218-A-A-219 or 179-A-A-180 and 197-A-A-198 and 218-A-A-219. 1 Publication | 2 | |
Mutagenesisi | 211 | Y → D: Cytosolic. Loss of membrane localization in absence of brassinosteroid treatment. 1 Publication | 1 | |
Mutagenesisi | 211 | Y → F: Loss of cytosolic localization. No dissociation from the membrane upon brassinosteroid treatment. 1 Publication | 1 | |
Mutagenesisi | 218 – 219 | KR → AA: No effect on subcellular location. Cytosolic only; when associated with 209-A-A-210 or 179-A-A-180 and 197-A-A-198 and 209-A-A-210. 1 Publication | 2 | |
Mutagenesisi | 322 | C → A: No effect on phosphorylation and subcellular location. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000410475 | 1 – 337 | BRI1 kinase inhibitor 1Add BLAST | 337 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 211 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Phosphorylated on Tyr-211 in response to brassinosteroid perception, leading to its inactivation: once phosphorylated, displaced into the cytosol where it is inactive.2 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9FMZ0 |
PRIDEi | Q9FMZ0 |
ProteomicsDBi | 240353 |
PTM databases
iPTMneti | Q9FMZ0 |
Expressioni
Tissue specificityi
Expressed in leaves, petioles, shoot apices, hypocotyls, roots and flowers.1 Publication
Gene expression databases
ExpressionAtlasi | Q9FMZ0, baseline and differential |
Genevisiblei | Q9FMZ0, AT |
Interactioni
Subunit structurei
Interacts (via C-terminus) with BRI1 (via kinase domain).
1 PublicationBinary interactionsi
Q9FMZ0
With | #Exp. | IntAct |
---|---|---|
BRL1 [Q9ZWC8] | 4 | EBI-1111615,EBI-590903 |
GO - Molecular functioni
- protein heterodimerization activity Source: TAIR
Protein-protein interaction databases
BioGRIDi | 19534, 1 interactor |
IntActi | Q9FMZ0, 1 interactor |
STRINGi | 3702.AT5G42750.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9FMZ0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 70 | Ser-richAdd BLAST | 45 | |
Compositional biasi | 131 – 135 | Poly-Asn | 5 | |
Compositional biasi | 189 – 194 | Poly-Gln | 6 | |
Compositional biasi | 232 – 306 | Ser-richAdd BLAST | 75 |
Domaini
The c-terminal part (253-337) is necessary and sufficient for the interaction with BRI1.
Phylogenomic databases
eggNOGi | ENOG502QT0J, Eukaryota |
HOGENOMi | CLU_869899_0_0_1 |
InParanoidi | Q9FMZ0 |
OMAi | KKYIRML |
OrthoDBi | 1319803at2759 |
PhylomeDBi | Q9FMZ0 |
Family and domain databases
InterProi | View protein in InterPro IPR039620, BKI1/MAKR1/3/4 |
PANTHERi | PTHR33312, PTHR33312, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9FMZ0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METNLQQVKN SSQTFSEKQN PKQEASPSPI SSTCSSPSHD FSFTISLQPL
60 70 80 90 100
SSSSKHISPT LRSPSKTTSS YQQTDPFAVD LSPADEIFFH GHLLPLHLLS
110 120 130 140 150
HLPVSPRTST GSYNDGFTLP VKDILPDQPT NNNNNTENAI TNISTEAKDD
160 170 180 190 200
NTEDKAEGEI RVKTKPIKSF SLFGLSKWRK GFESNEREQE QQQQKIKKPM
210 220 230 240 250
SLDLSHAVKK YIRMLFQKRG NGTQFWNRRQ TSSYSFSSSL MGPNGNSKTM
260 270 280 290 300
INGSYNKRDL IRGRRGELFS APASMRTSPT NSGHLRVSTA GLSSSSGSTS
310 320 330
SSSSDSTMEE LQAAIQAAIA HCKNSSAVDR DDKVKDS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB007647 Genomic DNA Translation: BAB10631.1 CP002688 Genomic DNA Translation: AED94856.1 AY087216 mRNA Translation: AAM64772.1 BT033090 mRNA Translation: ACF04813.1 |
RefSeqi | NP_568610.1, NM_123639.3 |
Genome annotation databases
EnsemblPlantsi | AT5G42750.1; AT5G42750.1; AT5G42750 |
GeneIDi | 834284 |
Gramenei | AT5G42750.1; AT5G42750.1; AT5G42750 |
KEGGi | ath:AT5G42750 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB007647 Genomic DNA Translation: BAB10631.1 CP002688 Genomic DNA Translation: AED94856.1 AY087216 mRNA Translation: AAM64772.1 BT033090 mRNA Translation: ACF04813.1 |
RefSeqi | NP_568610.1, NM_123639.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4OH4 | X-ray | 2.25 | E/F | 306-325 | [»] | |
4Q5J | X-ray | 2.77 | E/F | 306-325 | [»] | |
SMRi | Q9FMZ0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 19534, 1 interactor |
IntActi | Q9FMZ0, 1 interactor |
STRINGi | 3702.AT5G42750.1 |
PTM databases
iPTMneti | Q9FMZ0 |
Proteomic databases
PaxDbi | Q9FMZ0 |
PRIDEi | Q9FMZ0 |
ProteomicsDBi | 240353 |
Genome annotation databases
EnsemblPlantsi | AT5G42750.1; AT5G42750.1; AT5G42750 |
GeneIDi | 834284 |
Gramenei | AT5G42750.1; AT5G42750.1; AT5G42750 |
KEGGi | ath:AT5G42750 |
Organism-specific databases
Araporti | AT5G42750 |
TAIRi | locus:2165472, AT5G42750 |
Phylogenomic databases
eggNOGi | ENOG502QT0J, Eukaryota |
HOGENOMi | CLU_869899_0_0_1 |
InParanoidi | Q9FMZ0 |
OMAi | KKYIRML |
OrthoDBi | 1319803at2759 |
PhylomeDBi | Q9FMZ0 |
Miscellaneous databases
PROi | PR:Q9FMZ0 |
Gene expression databases
ExpressionAtlasi | Q9FMZ0, baseline and differential |
Genevisiblei | Q9FMZ0, AT |
Family and domain databases
InterProi | View protein in InterPro IPR039620, BKI1/MAKR1/3/4 |
PANTHERi | PTHR33312, PTHR33312, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BKI1_ARATH | |
Accessioni | Q9FMZ0Primary (citable) accession number: Q9FMZ0 Secondary accession number(s): Q8LBG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 28, 2011 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references