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Protein

Putative pectate lyase 21

Gene

At5g55720

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion. Required for its activity.By similarity

Pathwayi: pectin degradation

This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Pectinesterase (PMEPCRF), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (At3g14310), Pectinesterase (AXX17_At5g47900), Pectinesterase (AXX17_At4g38050), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At3g43380), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (At5g26810), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Pectinesterase (F14I3.7), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (At3g49220), Probable pectinesterase 29 (PME29), Pectinesterase (AXX17_At4g38040), Pectinesterase (At5g20860), Probable pectinesterase 8 (PME8), Uncharacterized protein (AXX17_At5g27870), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Pectinesterase QRT1 (QRT1), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase (AXX17_At3g53530), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Pectinesterase 5 (PME5), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Probable pectinesterase 67 (PME67), Probable pectinesterase 56 (PME56), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Uncharacterized protein (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (PME44), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase (AXX17_At2g33420), Pectinesterase, Pectinesterase (At4g15980), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Uncharacterized protein (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Pectate lyase (At3g27400), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Pectate lyase (At5g04310), Probable pectate lyase 22 (At5g63180), Pectate lyase (At5g09280), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase, Pectate lyase, Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase, Pectate lyase (At3g09540), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (AXX17_At4g28670), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase, Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi189CalciumBy similarity1
Metal bindingi213CalciumBy similarity1
Metal bindingi217CalciumBy similarity1
Active sitei269Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G55720-MONOMER
UniPathwayiUPA00545; UER00824

Protein family/group databases

CAZyiPL1 Polysaccharide Lyase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pectate lyase 21 (EC:4.2.2.2)
Gene namesi
Ordered Locus Names:At5g55720
ORF Names:MDF20.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G55720
TAIRilocus:2162182 AT5G55720

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002488622 – 392Putative pectate lyase 21Add BLAST371

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FM66
PRIDEiQ9FM66

Expressioni

Gene expression databases

ExpressionAtlasiQ9FM66 baseline and differential
GenevisibleiQ9FM66 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G55720.1

Structurei

3D structure databases

ProteinModelPortaliQ9FM66
SMRiQ9FM66
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEMI Eukaryota
COG3866 LUCA
HOGENOMiHOG000237948
InParanoidiQ9FM66
KOiK01728
OMAiCEIDQAC
OrthoDBiEOG09360CLV
PhylomeDBiQ9FM66

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR018082 AmbAllergen
IPR002022 Pec_lyase
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
PfamiView protein in Pfam
PF00544 Pec_lyase_C, 1 hit
PRINTSiPR00807 AMBALLERGEN
SMARTiView protein in SMART
SM00656 Amb_all, 1 hit
SUPFAMiSSF51126 SSF51126, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FM66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIVCTFFLF LLNTSFAFAF AIPKPPIVRR LSTTVTSNST ASSCSANGNP
60 70 80 90 100
IDECWRCDEN WKDNRKNLAD CAVGFGRDSI GGRAGEFYTV TDSGDDNPLN
110 120 130 140 150
PTPGTLRYAA TQDQPLWIIF DRDMVIQLKQ DLQVASYKTI DGRGNNVQIA
160 170 180 190 200
YGPCLTLYKV SNIIINNLYI HDCVPVKRNA LSSLGGYSDG DGISIFESRD
210 220 230 240 250
IWIDHCTLEK CYDGLIDAVN GSTDITISNS YMLNHNEVML LGHSDEYSGD
260 270 280 290 300
RDMRVTIAFN YFGEGLVQRM PRCRHGYFHI VNNIYRDWKM YAIGGSANPT
310 320 330 340 350
IFSQGNVFIA SNNQFTKEVT KRESADGDEE WKEWNWKSEG DEMVNGAFFT
360 370 380 390
PSGKEDSPSY AKFSSMVARP ASLLKTTHPS VGVLSCEIDQ AC
Length:392
Mass (Da):43,682
Last modified:March 1, 2001 - v1
Checksum:i305F94A437C30ECB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009050 Genomic DNA Translation: BAB09239.1
CP002688 Genomic DNA Translation: AED96672.1
RefSeqiNP_200383.1, NM_124954.3
UniGeneiAt.29429

Genome annotation databases

EnsemblPlantsiAT5G55720.1; AT5G55720.1; AT5G55720
GeneIDi835666
GrameneiAT5G55720.1; AT5G55720.1; AT5G55720
KEGGiath:AT5G55720

Similar proteinsi

Entry informationi

Entry nameiPLY21_ARATH
AccessioniPrimary (citable) accession number: Q9FM66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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