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Protein

E3 ubiquitin-protein ligase SINA-like 11

Gene

At5g62800

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103Zinc 1By similarity1
Metal bindingi110Zinc 1By similarity1
Metal bindingi122Zinc 1By similarity1
Metal bindingi126Zinc 1By similarity1
Metal bindingi133Zinc 2By similarity1
Metal bindingi138Zinc 2By similarity1
Metal bindingi150Zinc 2By similarity1
Metal bindingi155Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri43 – 81RING-type; degenerateAdd BLAST39
Zinc fingeri98 – 156SIAH-typePROSITE-ProRule annotationAdd BLAST59

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SINA-like 11 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase SINA-like 11Curated
Seven in absentia-like protein 11
Gene namesi
Ordered Locus Names:At5g62800
ORF Names:MQB2.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G62800

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002992001 – 314E3 ubiquitin-protein ligase SINA-like 11Add BLAST314

Expressioni

Gene expression databases

ExpressionAtlasiQ9FM14 baseline and differential

Structurei

3D structure databases

ProteinModelPortaliQ9FM14
SMRiQ9FM14
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 280SBDBy similarityAdd BLAST186

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri43 – 81RING-type; degenerateAdd BLAST39
Zinc fingeri98 – 156SIAH-typePROSITE-ProRule annotationAdd BLAST59

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiQ9FM14
KOiK04506
OMAiLINDHES
PhylomeDBiQ9FM14

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS51081 ZF_SIAH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FM14-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEDSNSHPQN QTSKRKSSHP QKKQRMENET RSAKLLDLDV LDCPVCFEPL
60 70 80 90 100
TIPTFQCDDG HIVCNFCFAK VSNKCPGPGC DLPIGNKRCF AMERVLESAF
110 120 130 140 150
VPCQNTEFGC TKSVSYEKVS SHEKECNYSQ CSCPNLECNY TGSYNIIYGH
160 170 180 190 200
FMRRHLYNST IVSSKWGYST VDVLINIKEK VSVLWESRQK LLFVVQCFKE
210 220 230 240 250
RHGVYVTVRR IAPPASEFKK FSYRLSYSID GHNVTYESPE VKRLLEVNSQ
260 270 280 290 300
IPDDSFMFVP NCLLHGFLIK PANEVQQVTI AQETVMEDPP TSLFKNSVPI
310
REDQIQNAIT NSIR
Length:314
Mass (Da):35,880
Last modified:September 11, 2007 - v2
Checksum:i8AEF52BE2E55D964
GO

Sequence cautioni

The sequence AED97659 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAB10849 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009053 Genomic DNA Translation: BAB10849.1 Sequence problems.
CP002688 Genomic DNA Translation: AED97659.1 Sequence problems.
CP002688 Genomic DNA Translation: ANM69719.1
RefSeqiNP_001331378.1, NM_001345561.1
NP_201086.1, NM_125675.2
UniGeneiAt.29032

Genome annotation databases

EnsemblPlantsiAT5G62800.2; AT5G62800.2; AT5G62800
GeneIDi836401
GrameneiAT5G62800.2; AT5G62800.2; AT5G62800
KEGGiath:AT5G62800

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009053 Genomic DNA Translation: BAB10849.1 Sequence problems.
CP002688 Genomic DNA Translation: AED97659.1 Sequence problems.
CP002688 Genomic DNA Translation: ANM69719.1
RefSeqiNP_001331378.1, NM_001345561.1
NP_201086.1, NM_125675.2
UniGeneiAt.29032

3D structure databases

ProteinModelPortaliQ9FM14
SMRiQ9FM14
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G62800.2; AT5G62800.2; AT5G62800
GeneIDi836401
GrameneiAT5G62800.2; AT5G62800.2; AT5G62800
KEGGiath:AT5G62800

Organism-specific databases

AraportiAT5G62800

Phylogenomic databases

InParanoidiQ9FM14
KOiK04506
OMAiLINDHES
PhylomeDBiQ9FM14

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q9FM14

Gene expression databases

ExpressionAtlasiQ9FM14 baseline and differential

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIL11_ARATH
AccessioniPrimary (citable) accession number: Q9FM14
Secondary accession number(s): F4K7T7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 7, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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