UniProtKB - Q9FLW2 (FRO5_ARATH)
Ferric reduction oxidase 5
FRO5
Functioni
Ferric chelate reductase probably involved in iron reduction in shoots. May participate in the transport of electrons to a Fe3+ ion via FAD and heme intermediates. May act in iron metabolism in reproductive organs (PubMed:16006655).
May function as root surface cupric chelate reductase and participate in the reduction of Cu2+, for Cu+ acquisition via Cu+ transporters in response to copper deficiency (PubMed:22374396).
2 PublicationsCatalytic activityi
- EC:1.16.1.7
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 200 | Iron (heme axial ligand)Sequence analysis | 1 | |
Metal bindingi | 214 | Iron (heme axial ligand)Sequence analysis | 1 | |
Metal bindingi | 274 | Iron (heme axial ligand)Sequence analysis | 1 | |
Metal bindingi | 287 | Iron (heme axial ligand)Sequence analysis | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 362 – 365 | FADSequence analysis | 4 | |
Nucleotide bindingi | 408 – 411 | NADSequence analysis | 4 |
GO - Molecular functioni
- ferric-chelate reductase (NADH) activity Source: UniProtKB-EC
- ferric-chelate reductase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- ion transport Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Ion transport, Transport |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | ARA:AT5G23990-MONOMER |
BRENDAi | 1.16.1.10, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Ferric reduction oxidase 5 (EC:1.16.1.7)Short name: AtFRO5 Alternative name(s): Ferric-chelate reductase 5 |
Gene namesi | Name:FRO5 Ordered Locus Names:At5g23990 ORF Names:MZF18.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G23990 |
TAIRi | locus:2178687, AT5G23990 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 29 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 30 – 54 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 55 – 73 | HelicalBy similarityAdd BLAST | 19 | |
Topological domaini | 74 – 106 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 107 – 130 | HelicalBy similarityAdd BLAST | 24 | |
Topological domaini | 131 – 198 | ExtracellularSequence analysisAdd BLAST | 68 | |
Transmembranei | 199 – 222 | HelicalBy similarityAdd BLAST | 24 | |
Topological domaini | 223 – 272 | CytoplasmicSequence analysisAdd BLAST | 50 | |
Transmembranei | 273 – 297 | HelicalBy similarityAdd BLAST | 25 | |
Topological domaini | 298 – 319 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 320 – 340 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 341 – 533 | CytoplasmicSequence analysisAdd BLAST | 193 | |
Transmembranei | 534 – 556 | HelicalBy similarityAdd BLAST | 23 | |
Topological domaini | 557 – 576 | ExtracellularSequence analysisAdd BLAST | 20 | |
Transmembranei | 577 – 598 | HelicalBy similarityAdd BLAST | 22 | |
Topological domaini | 599 – 707 | CytoplasmicSequence analysisAdd BLAST | 109 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413203 | 1 – 707 | Ferric reduction oxidase 5Add BLAST | 707 |
Proteomic databases
ProteomicsDBi | 230045 [Q9FLW2-1] |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
ExpressionAtlasi | Q9FLW2, baseline and differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 165 – 284 | Ferric oxidoreductaseAdd BLAST | 120 | |
Domaini | 313 – 416 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 104 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 608 – 630 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
InParanoidi | Q9FLW2 |
PhylomeDBi | Q9FLW2 |
Family and domain databases
Gene3Di | 3.40.50.80, 2 hits |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNMRSLVKM LMVVLFLGWI FVWIMISTNR FQNIWTPKLA KYLKTTYFGP
60 70 80 90 100
QGMNLVLLTV PMMFIAVLSC VYLHTQKQPS QTQSLYKCRE WKVKGRMGRV
110 120 130 140 150
MMVMNPLGIV TATELTFSLL FLALLVWALS NYLYLSYHVH LHNHDNANDM
160 170 180 190 200
CRWQAKFRAF GLRIGYVGHY CWAFLFFPVT RASTILPLVG LTSESSIKYH
210 220 230 240 250
IWLGHVSNFC FLVHTVVFLI YWAMVNKLME TFAWNATYVP NLAGTIAMVI
260 270 280 290 300
GIAIWVTSLP SFRRKKFEIF FYTHHLYGLY IVFYAIHVGD SWFCMILPNI
310 320 330 340 350
FLFFIDRYLR FLQSTKRSRL VSAKILPSDN LELTFAKTSG LHYTPTSILF
360 370 380 390 400
LHVPSISKLQ WHPFTITSSS NLEKDTLSVV IRKQGSWTQK LYTHLSSSID
410 420 430 440 450
SLEVSTEGPY GPNSFDVSRH DSLILVGGGS GVTPFISVIR ELIFQSQNRS
460 470 480 490 500
TKLPNVLLVC AFKNYHDLAF LDLIFPSDIS VSDISKLNLR IEAYITREDK
510 520 530 540 550
KPETTDDHKL LQTKWFKPQP LDSPISPVLG PNNFLWLGVV ILSSFVMFLL
560 570 580 590 600
LIGIVTRYYI YPVDHNTGSI YNFTYRVLWV MFLGCVCIFI SSSIIFLWRK
610 620 630 640 650
KENKEGDKDS KKQVQSVEFQ TPTSSPGSWF HGHERELESV PYQSIVQATS
660 670 680 690 700
VHFGSKPNLK KILFEAEGSE DVGVMVCGPK KMRHEVAKIC SSGLAKNLHF
EAISFNW
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8B9Z4 | A0A1P8B9Z4_ARATH | Ferric reduction oxidase 5 | FRO5 ATFRO5, FERRIC REDUCTION OXIDASE 5, ferric reduction oxidase 5, MZF18.13, MZF18_13 | 567 | Annotation score: | ||
A0A178UNE5 | A0A178UNE5_ARATH | Ferric reduction oxidase 5 | FRO5 ATFRO5, FERRIC REDUCTION OXIDASE 5, ferric reduction oxidase 5, MZF18.13, MZF18_13 | 699 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041874 | 42 – 154 | YLKTT…MCRWQ → HESCATHGSNDVHCGSELCL FAYPKATLPNSKRMEGKREN GEGDDGYEPIGDSDSHRVDF FSV in isoform 2. CuratedAdd BLAST | 113 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009056 Genomic DNA Translation: BAB08722.1 CP002688 Genomic DNA Translation: AED93243.1 |
RefSeqi | NP_197787.1, NM_122304.1 [Q9FLW2-2] |
Genome annotation databases
EnsemblPlantsi | AT5G23990.1; AT5G23990.1; AT5G23990 [Q9FLW2-2] |
GeneIDi | 832464 |
Gramenei | AT5G23990.1; AT5G23990.1; AT5G23990 [Q9FLW2-2] |
KEGGi | ath:AT5G23990 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009056 Genomic DNA Translation: BAB08722.1 CP002688 Genomic DNA Translation: AED93243.1 |
RefSeqi | NP_197787.1, NM_122304.1 [Q9FLW2-2] |
3D structure databases
SMRi | Q9FLW2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 17739, 2 interactors |
Proteomic databases
ProteomicsDBi | 230045 [Q9FLW2-1] |
Genome annotation databases
EnsemblPlantsi | AT5G23990.1; AT5G23990.1; AT5G23990 [Q9FLW2-2] |
GeneIDi | 832464 |
Gramenei | AT5G23990.1; AT5G23990.1; AT5G23990 [Q9FLW2-2] |
KEGGi | ath:AT5G23990 |
Organism-specific databases
Araporti | AT5G23990 |
TAIRi | locus:2178687, AT5G23990 |
Phylogenomic databases
InParanoidi | Q9FLW2 |
PhylomeDBi | Q9FLW2 |
Enzyme and pathway databases
BioCyci | ARA:AT5G23990-MONOMER |
BRENDAi | 1.16.1.10, 399 |
Miscellaneous databases
PROi | PR:Q9FLW2 |
Gene expression databases
ExpressionAtlasi | Q9FLW2, baseline and differential |
Family and domain databases
Gene3Di | 3.40.50.80, 2 hits |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FRO5_ARATH | |
Accessioni | Q9FLW2Primary (citable) accession number: Q9FLW2 Secondary accession number(s): F4KFN9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families