UniProtKB - Q9FLQ4 (ODO2A_ARATH)
Protein
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial
Gene
At5g55070
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity
Catalytic activityi
- (R)-N6-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N6-(S8-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoAEC:2.3.1.61
Cofactori
(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity
: L-lysine degradation via saccharopine pathway Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes glutaryl-CoA from L-lysine.Proteins known to be involved in the 6 steps of the subpathway in this organism are:
- Lysine ketoglutarate reductase (C24_LOCUS20033), Lysine ketoglutarate reductase (LKR), Lysine ketoglutarate reductase (AXX17_At4g37980), Lysine ketoglutarate reductase (AN1_LOCUS20138), Alpha-aminoadipic semialdehyde synthase (LKR/SDH)
- Lysine ketoglutarate reductase (C24_LOCUS20033), Lysine ketoglutarate reductase (LKR), Lysine ketoglutarate reductase (AXX17_At4g37980), Lysine ketoglutarate reductase (AN1_LOCUS20138), Alpha-aminoadipic semialdehyde synthase (LKR/SDH)
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
- Dihydrolipoyllysine-residue succinyltransferase (C24_LOCUS25606), Dihydrolipoyllysine-residue succinyltransferase (AXX17_At4g31060), Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial (At4g26910), Dihydrolipoyllysine-residue succinyltransferase (AXX17_At5g54170), Dihydrolipoyllysine-residue succinyltransferase (AN1_LOCUS25778), Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial (At5g55070), Dihydrolipoyllysine-residue succinyltransferase (AXX17_At4g31060)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutaryl-CoA from L-lysine, the pathway L-lysine degradation via saccharopine pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 435 | By similarity | 1 | |
Active sitei | 439 | By similarity | 1 |
GO - Molecular functioni
- dihydrolipoyllysine-residue succinyltransferase activity Source: UniProtKB-EC
- zinc ion binding Source: TAIR
GO - Biological processi
- L-lysine catabolic process to acetyl-CoA via saccharopine Source: UniProtKB-UniPathway
- response to oxidative stress Source: TAIR
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Tricarboxylic acid cycle |
Enzyme and pathway databases
BioCyci | ARA:AT5G55070-MONOMER |
UniPathwayi | UPA00868;UER00840 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial (EC:2.3.1.61)Alternative name(s): 2-oxoglutarate dehydrogenase complex component E2-1 Short name: OGDC-E2-1 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1 E2K-1 |
Gene namesi | Ordered Locus Names:At5g55070 ORF Names:MCO15.2 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G55070 |
TAIRi | locus:2161670, AT5G55070 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication1 Publication
Cytosol
- cytosolic ribosome Source: TAIR
Mitochondrion
- mitochondrion Source: TAIR
Other locations
- oxoglutarate dehydrogenase complex Source: InterPro
- plastid Source: TAIR
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 86 | Mitochondrion1 PublicationAdd BLAST | 86 | |
ChainiPRO_0000399512 | 87 – 464 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrialAdd BLAST | 378 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 134 | N6-lipoyllysinePROSITE-ProRule annotation | 1 |
Proteomic databases
PaxDbi | Q9FLQ4 |
PRIDEi | Q9FLQ4 |
ProteomicsDBi | 250874 |
PTM databases
MetOSitei | Q9FLQ4 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9FLQ4, baseline and differential |
Genevisiblei | Q9FLQ4, AT |
Interactioni
Subunit structurei
Forms a 24-polypeptide structural core with octahedral symmetry.
By similarityProtein-protein interaction databases
BioGRIDi | 20842, 11 interactors |
IntActi | Q9FLQ4, 1 interactor |
STRINGi | 3702.AT5G55070.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 93 – 168 | Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST | 76 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 179 – 231 | Pro-richAdd BLAST | 53 |
Sequence similaritiesi
Belongs to the 2-oxoacid dehydrogenase family.Curated
Keywords - Domaini
Lipoyl, Transit peptidePhylogenomic databases
eggNOGi | KOG0559, Eukaryota |
HOGENOMi | CLU_016733_0_2_1 |
InParanoidi | Q9FLQ4 |
OMAi | DMAGATF |
OrthoDBi | 850255at2759 |
PhylomeDBi | Q9FLQ4 |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR011053, Single_hybrid_motif IPR006255, SucB |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR01347, sucB, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9FLQ4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMLRAVFRRA SIRGSSSASG LGKSLQSSRV AVSAQFHSVS ATETLVPRGN
60 70 80 90 100
HAHSFHHRSC PGCPDCSRTI INGYQGTALQ RWVRPFSSDS GDVVEAVVPH
110 120 130 140 150
MGESITDGTL AAFLKKPGDR VEADEAIAQI ETDKVTIDIA SPASGVIQEF
160 170 180 190 200
LVKEGDTVEP GNKVARISTS ADAVSHVAPS EKAPEKPAPK PSPPAEKPKV
210 220 230 240 250
ESTKVAEKPK APSPPPPPPS KQSAKEPQLP PKDRERRVPM TRLRKRVATR
260 270 280 290 300
LKDSQNTFAL LTTFNEVDMT NLMKLRSQYK DAFLEKHGVK LGLMSGFIKA
310 320 330 340 350
AVSALQHQPV VNAVIDGDDI IYRDYVDISI AVGTSKGLVV PVIRDADKMN
360 370 380 390 400
FADIEKTING LAKKATEGTI SIDEMAGGSF TVSNGGVYGS LISTPIINPP
410 420 430 440 450
QSAILGMHSI VQRPMVVGGS VVPRPMMYVA LTYDHRLIDG REAVYFLRRI
460
KDVVEDPQRL LLDI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 – 34 | VAVSA → LVASS in CAA11553 (Ref. 1) Curated | 5 | |
Sequence conflicti | 70 | I → V in CAA11553 (Ref. 1) Curated | 1 | |
Sequence conflicti | 74 | Y → F in CAA11553 (Ref. 1) Curated | 1 | |
Sequence conflicti | 218 – 219 | Missing in CAA11553 (Ref. 1) Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223803 mRNA Translation: CAA11553.1 AB010071 Genomic DNA Translation: BAB08576.1 CP002688 Genomic DNA Translation: AED96577.1 CP002688 Genomic DNA Translation: ANM68999.1 AY042897 mRNA Translation: AAK68837.1 AY128726 mRNA Translation: AAM91126.1 |
RefSeqi | NP_001330709.1, NM_001345105.1 NP_200318.1, NM_124889.5 |
Genome annotation databases
EnsemblPlantsi | AT5G55070.1; AT5G55070.1; AT5G55070 AT5G55070.2; AT5G55070.2; AT5G55070 |
GeneIDi | 835598 |
Gramenei | AT5G55070.1; AT5G55070.1; AT5G55070 AT5G55070.2; AT5G55070.2; AT5G55070 |
KEGGi | ath:AT5G55070 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223803 mRNA Translation: CAA11553.1 AB010071 Genomic DNA Translation: BAB08576.1 CP002688 Genomic DNA Translation: AED96577.1 CP002688 Genomic DNA Translation: ANM68999.1 AY042897 mRNA Translation: AAK68837.1 AY128726 mRNA Translation: AAM91126.1 |
RefSeqi | NP_001330709.1, NM_001345105.1 NP_200318.1, NM_124889.5 |
3D structure databases
SMRi | Q9FLQ4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 20842, 11 interactors |
IntActi | Q9FLQ4, 1 interactor |
STRINGi | 3702.AT5G55070.1 |
PTM databases
MetOSitei | Q9FLQ4 |
Proteomic databases
PaxDbi | Q9FLQ4 |
PRIDEi | Q9FLQ4 |
ProteomicsDBi | 250874 |
Genome annotation databases
EnsemblPlantsi | AT5G55070.1; AT5G55070.1; AT5G55070 AT5G55070.2; AT5G55070.2; AT5G55070 |
GeneIDi | 835598 |
Gramenei | AT5G55070.1; AT5G55070.1; AT5G55070 AT5G55070.2; AT5G55070.2; AT5G55070 |
KEGGi | ath:AT5G55070 |
Organism-specific databases
Araporti | AT5G55070 |
TAIRi | locus:2161670, AT5G55070 |
Phylogenomic databases
eggNOGi | KOG0559, Eukaryota |
HOGENOMi | CLU_016733_0_2_1 |
InParanoidi | Q9FLQ4 |
OMAi | DMAGATF |
OrthoDBi | 850255at2759 |
PhylomeDBi | Q9FLQ4 |
Enzyme and pathway databases
UniPathwayi | UPA00868;UER00840 |
BioCyci | ARA:AT5G55070-MONOMER |
Miscellaneous databases
PROi | PR:Q9FLQ4 |
Gene expression databases
ExpressionAtlasi | Q9FLQ4, baseline and differential |
Genevisiblei | Q9FLQ4, AT |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR011053, Single_hybrid_motif IPR006255, SucB |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR01347, sucB, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ODO2A_ARATH | |
Accessioni | Q9FLQ4Primary (citable) accession number: Q9FLQ4 Secondary accession number(s): Q9ZRQ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families