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Entry version 145 (31 Jul 2019)
Sequence version 1 (01 Mar 2001)
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Protein

Calcium-dependent protein kinase 28

Gene

CPK28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger (Probable). Acts as developmentally controlled regulator for coordinated stem elongation and vascular development. Acts as key component which contributes to the developmental switch that establishes the transition from vegetative to reproductive growth (PubMed:23252373). Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling. Interacts with and phosphorylates the kinase BIK1, a central rate-limiting kinase in PTI signaling. Facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several PAMPs. Its kinase activity is necessary and sufficient for its function in PTI signaling (PubMed:25525792).1 Publication2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by calcium (PubMed:23252373). Autophosphorylation plays an important role in the regulation of the kinase activity (PubMed:23252373).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei91ATPPROSITE-ProRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei188Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi68 – 76ATPPROSITE-ProRule annotation9
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position(s) of the calcium-binding region(s) within the protein. One common calcium-binding motif is the EF-hand, but other calcium-binding motifs also exist.<p><a href='/help/ca_bind' target='_top'>More...</a></p>Calcium bindingi378 – 3891PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi415 – 4262PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi457 – 4683PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi487 – 4984PROSITE-ProRule annotationAdd BLAST12

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processGrowth regulation, Plant defense
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Calcium-dependent protein kinase 281 Publication (EC:2.7.11.11 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CPK281 Publication
Ordered Locus Names:At5g66210
ORF Names:K2A18.29
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

Organism-specific databases

Arabidopsis Information Portal

More...
Araporti
AT5G66210

The Arabidopsis Information Resource

More...
TAIRi
locus:2156947 AT5G66210

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Severe growth defect in shoot elongation. Severe growth defect of flowering stem.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi188D → A: Abolishes kinase activity. 1 Publication1
Mutagenesisi245S → L in mod1; abolishes kinase activity; when associated with V-295. 1 Publication1
Mutagenesisi295A → V in mod1; abolishes kinase activity; when associated with L-245. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCurated
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003633502 – 523Calcium-dependent protein kinase 28Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section specifies the position(s) and the type of covalently attached lipid group(s).<p><a href='/help/lipid' target='_top'>More...</a></p>Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi4S-palmitoyl cysteineBy similarity1
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei228Phosphoserine1 Publication1
Modified residuei318Phosphoserine1 Publication1
Modified residuei495Phosphoserine1 Publication1
Modified residuei515PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9FKW4

PRoteomics IDEntifications database

More...
PRIDEi
Q9FKW4

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q9FKW4

SwissPalm database of S-palmitoylation events

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SwissPalmi
Q9FKW4

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in vascular and meristematic tissues throughout plant development.1 Publication

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q9FKW4 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q9FKW4 AT

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with BIK1.

1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
3702.AT5G66210.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9FKW4

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini62 – 322Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini365 – 401EF-hand 1PROSITE-ProRule annotationAdd BLAST37
Domaini402 – 437EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini438 – 479EF-hand 3PROSITE-ProRule annotationAdd BLAST42
Domaini480 – 509EF-hand 4PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni328 – 358Autoinhibitory domainBy similarityAdd BLAST31

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (328-358) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG0032 Eukaryota
ENOG410XRMJ LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000233030

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q9FKW4

KEGG Orthology (KO)

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KOi
K13412

Identification of Orthologs from Complete Genome Data

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OMAi
NIMFESK

Database of Orthologous Groups

More...
OrthoDBi
330091at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9FKW4

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13499 EF-hand_7, 2 hits
PF00069 Pkinase, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00054 EFh, 4 hits
SM00220 S_TKc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9FKW4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGVCFSAIRV TGASSSRRSS QTKSKAAPTP IDTKASTKRR TGSIPCGKRT
60 70 80 90 100
DFGYSKDFHD HYTIGKLLGH GQFGYTYVAI HRPNGDRVAV KRLDKSKMVL
110 120 130 140 150
PIAVEDVKRE VQILIALSGH ENVVQFHNAF EDDDYVYIVM ELCEGGELLD
160 170 180 190 200
RILSKKGNRY SEKDAAVVVR QMLKVAGECH LHGLVHRDMK PENFLFKSAQ
210 220 230 240 250
LDSPLKATDF GLSDFIKPGK RFHDIVGSAY YVAPEVLKRR SGPESDVWSI
260 270 280 290 300
GVITYILLCG RRPFWDRTED GIFKEVLRNK PDFSRKPWAT ISDSAKDFVK
310 320 330 340 350
KLLVKDPRAR LTAAQALSHA WVREGGNATD IPVDISVLNN LRQFVRYSRL
360 370 380 390 400
KQFALRALAS TLDEAEISDL RDQFDAIDVD KNGVISLEEM RQALAKDLPW
410 420 430 440 450
KLKDSRVAEI LEAIDSNTDG LVDFTEFVAA ALHVHQLEEH DSEKWQLRSR
460 470 480 490 500
AAFEKFDLDK DGYITPEELR MHTGLRGSID PLLDEADIDR DGKISLHEFR
510 520
RLLRTASISS QRAPSPAGHR NLR
Length:523
Mass (Da):58,972
Last modified:March 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC4211CAA1F22619D
GO
Isoform 2 (identifier: Q9FKW4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     472-488: HTGLRGSIDPLLDEADI → AQQNKERKWSENPIDEI
     489-523: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:488
Mass (Da):55,254
Checksum:iFCA1D0D1CEF861A1
GO
Isoform 3 (identifier: Q9FKW4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-435: IDSNTDGLVDFTEFVAAALHVH → VVIFLFSIAIASLGVSEGDVVS
     436-523: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:435
Mass (Da):48,644
Checksum:i52E82FE60A7271F2
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1P8BD52A0A1P8BD52_ARATH
Calcium-dependent protein kinase 28
CPK28 At5g66210, K2A18.29, K2A18_29
473Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti34K → E in BAF01180 (Ref. 4) Curated1
Sequence conflicti183G → D in AAM63052 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_036292414 – 435IDSNT…ALHVH → VVIFLFSIAIASLGVSEGDV VS in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_036293436 – 523Missing in isoform 3. 1 PublicationAdd BLAST88
Alternative sequenceiVSP_036294472 – 488HTGLR…DEADI → AQQNKERKWSENPIDEI in isoform 2. CuratedAdd BLAST17
Alternative sequenceiVSP_036295489 – 523Missing in isoform 2. CuratedAdd BLAST35

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AB011474 Genomic DNA Translation: BAB10426.1
CP002688 Genomic DNA Translation: AED98177.1
CP002688 Genomic DNA Translation: AED98178.1
CP002688 Genomic DNA Translation: AED98179.1
CP002688 Genomic DNA Translation: AED98180.1
CP002688 Genomic DNA Translation: ANM69530.1
AY139991 mRNA Translation: AAM98133.1
BT010380 mRNA Translation: AAQ56823.1
AK227052 mRNA Translation: BAE99112.1
AK229317 mRNA Translation: BAF01180.1
AY085837 mRNA Translation: AAM63052.1

NCBI Reference Sequences

More...
RefSeqi
NP_001078806.1, NM_001085337.1 [Q9FKW4-2]
NP_001119508.1, NM_001126036.2 [Q9FKW4-3]
NP_001331200.1, NM_001345752.1 [Q9FKW4-3]
NP_201422.1, NM_126019.4 [Q9FKW4-1]
NP_851280.1, NM_180949.3 [Q9FKW4-1]

Genome annotation databases

Ensembl plant genome annotation project

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EnsemblPlantsi
AT5G66210.1; AT5G66210.1; AT5G66210 [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210 [Q9FKW4-1]
AT5G66210.3; AT5G66210.3; AT5G66210 [Q9FKW4-2]
AT5G66210.4; AT5G66210.4; AT5G66210 [Q9FKW4-3]
AT5G66210.6; AT5G66210.6; AT5G66210 [Q9FKW4-3]

Database of genes from NCBI RefSeq genomes

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GeneIDi
836753

Gramene; a comparative resource for plants

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Gramenei
AT5G66210.1; AT5G66210.1; AT5G66210 [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210 [Q9FKW4-1]
AT5G66210.3; AT5G66210.3; AT5G66210 [Q9FKW4-2]
AT5G66210.4; AT5G66210.4; AT5G66210 [Q9FKW4-3]
AT5G66210.6; AT5G66210.6; AT5G66210 [Q9FKW4-3]

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
ath:AT5G66210

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011474 Genomic DNA Translation: BAB10426.1
CP002688 Genomic DNA Translation: AED98177.1
CP002688 Genomic DNA Translation: AED98178.1
CP002688 Genomic DNA Translation: AED98179.1
CP002688 Genomic DNA Translation: AED98180.1
CP002688 Genomic DNA Translation: ANM69530.1
AY139991 mRNA Translation: AAM98133.1
BT010380 mRNA Translation: AAQ56823.1
AK227052 mRNA Translation: BAE99112.1
AK229317 mRNA Translation: BAF01180.1
AY085837 mRNA Translation: AAM63052.1
RefSeqiNP_001078806.1, NM_001085337.1 [Q9FKW4-2]
NP_001119508.1, NM_001126036.2 [Q9FKW4-3]
NP_001331200.1, NM_001345752.1 [Q9FKW4-3]
NP_201422.1, NM_126019.4 [Q9FKW4-1]
NP_851280.1, NM_180949.3 [Q9FKW4-1]

3D structure databases

SMRiQ9FKW4
ModBaseiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G66210.1

PTM databases

iPTMnetiQ9FKW4
SwissPalmiQ9FKW4

Proteomic databases

PaxDbiQ9FKW4
PRIDEiQ9FKW4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G66210.1; AT5G66210.1; AT5G66210 [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210 [Q9FKW4-1]
AT5G66210.3; AT5G66210.3; AT5G66210 [Q9FKW4-2]
AT5G66210.4; AT5G66210.4; AT5G66210 [Q9FKW4-3]
AT5G66210.6; AT5G66210.6; AT5G66210 [Q9FKW4-3]
GeneIDi836753
GrameneiAT5G66210.1; AT5G66210.1; AT5G66210 [Q9FKW4-1]
AT5G66210.2; AT5G66210.2; AT5G66210 [Q9FKW4-1]
AT5G66210.3; AT5G66210.3; AT5G66210 [Q9FKW4-2]
AT5G66210.4; AT5G66210.4; AT5G66210 [Q9FKW4-3]
AT5G66210.6; AT5G66210.6; AT5G66210 [Q9FKW4-3]
KEGGiath:AT5G66210

Organism-specific databases

AraportiAT5G66210
TAIRilocus:2156947 AT5G66210

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
HOGENOMiHOG000233030
InParanoidiQ9FKW4
KOiK13412
OMAiNIMFESK
OrthoDBi330091at2759
PhylomeDBiQ9FKW4

Miscellaneous databases

Protein Ontology

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PROi
PR:Q9FKW4

Gene expression databases

ExpressionAtlasiQ9FKW4 baseline and differential
GenevisibleiQ9FKW4 AT

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCDPKS_ARATH
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9FKW4
Secondary accession number(s): A8MQP5, Q0WNW9, Q8LDS1
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: March 1, 2001
Last modified: July 31, 2019
This is version 145 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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