UniProtKB - Q9FJP9 (SDHB3_ARATH)
Protein
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial
Gene
SDH2-3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity
Catalytic activityi
- EC:1.3.5.1
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity
- [3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).CuratedProteins known to be involved in this subpathway in this organism are:
- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial (SDH2-3), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial (SDH2-1), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial (SDH2-2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit 1, mitochondrial (SDH1-1), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (AN1_LOCUS8206), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (AXX17_At5g38380), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (AXX17_At3g29870), Iron-sulfur subunit of complex II (AXX17_At5g64910), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDH2-2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (AXX17_At2g13790), Iron-sulfur subunit of complex II (SDH2-3), Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (SDH1-2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (AXX17_At5g66760), Iron-sulfur subunit of complex II
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 120 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 125 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 140 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 212 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 215 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 218 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 222 | Iron-sulfur 3 (3Fe-4S)By similarity | 1 | |
Binding sitei | 227 | Ubiquinone; shared with SdhD subunitBy similarity | 1 | |
Metal bindingi | 270 | Iron-sulfur 3 (3Fe-4S)By similarity | 1 | |
Metal bindingi | 276 | Iron-sulfur 3 (3Fe-4S)By similarity | 1 | |
Metal bindingi | 280 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- electron transfer activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
- succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
- succinate dehydrogenase activity Source: TAIR
GO - Biological processi
- aerobic respiration Source: GO_Central
- mitochondrial electron transport, succinate to ubiquinone Source: TAIR
- respiratory electron transport chain Source: GO_Central
- tricarboxylic acid cycle Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport, Tricarboxylic acid cycle |
Ligand | 2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER01006 |
Names & Taxonomyi
Protein namesi | Recommended name: Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial (EC:1.3.5.1)Alternative name(s): Iron-sulfur subunit of complex II Short name: Ip |
Gene namesi | Name:SDH2-3 Ordered Locus Names:At5g65165 ORF Names:MQN23.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G65165 |
TAIRi | locus:504954878, AT5G65165 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrion Source: TAIR
Other locations
- respiratory chain complex II Source: UniProtKB
- succinate dehydrogenase complex Source: TAIR
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 22 | MitochondrionSequence analysisAdd BLAST | 22 | |
ChainiPRO_0000247597 | 23 – 309 | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrialAdd BLAST | 287 |
Proteomic databases
PaxDbi | Q9FJP9 |
PRIDEi | Q9FJP9 |
ProteomicsDBi | 232778 |
Expressioni
Developmental stagei
Expressed during seed development. Detected during seed maturation and decreased during germination.1 Publication
Gene expression databases
ExpressionAtlasi | Q9FJP9, baseline and differential |
Interactioni
Subunit structurei
Component of complex II composed of eight subunits in plants: four classical SDH subunits SDH1, SDH2, SDH3 and SDH4 (a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.), as well as four subunits unknown in mitochondria from bacteria and heterotrophic eukaryotes.
1 PublicationProtein-protein interaction databases
BioGRIDi | 21882, 4 interactors |
STRINGi | 3702.AT5G65165.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 69 – 160 | 2Fe-2S ferredoxin-typeAdd BLAST | 92 | |
Domaini | 202 – 232 | 4Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 31 |
Sequence similaritiesi
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3049, Eukaryota |
HOGENOMi | CLU_044838_0_3_1 |
InParanoidi | Q9FJP9 |
OMAi | YDQYASI |
OrthoDBi | 1264157at2759 |
PhylomeDBi | Q9FJP9 |
Family and domain databases
Gene3Di | 1.10.1060.10, 1 hit 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR006058, 2Fe2S_fd_BS IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR012675, Beta-grasp_dom_sf IPR009051, Helical_ferredxn IPR004489, Succ_DH/fum_Rdtase_Fe-S IPR025192, Succ_DH/fum_Rdtase_N |
Pfami | View protein in Pfam PF13085, Fer2_3, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR00384, dhsB, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9FJP9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSVLRLLGR RICNPAAEKV RLSSSLSGGG DFPILNGHKA AQDLSKDTLK
60 70 80 90 100
SQDITKEKEG QHKEVKKEFK IYRWNPDKPN SKPFLQSFFV DLSSCGPMVL
110 120 130 140 150
DVLQKIKAED DASLSYRRSC REGICGSCSM NIDGTNTVAC LKPINPNTSK
160 170 180 190 200
PTIITPLPHM YVIKDLVVDL TNFYQQYKSM EPWLKTRKPP KDGREHRQSP
210 220 230 240 250
KDRKKLDGLY ECILCACCTT SCPSYWWNPE EFPGPAALLQ AYRWISDSRD
260 270 280 290 300
EYREERLQAI TESETKVYRC RAIKNCTATC PKGLNPASAI LKMKSKHLLS
DPLVRTESV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BF67 | A0A1P8BF67_ARATH | Iron-sulfur subunit of complex II | SDH2-3 succinate dehydrogenase 2-3, At5g65165 | 248 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278912 mRNA Translation: CAC19857.1 AB013395 Genomic DNA Translation: BAB11652.1 CP002688 Genomic DNA Translation: AED98011.1 |
RefSeqi | NP_680465.2, NM_148160.3 |
Genome annotation databases
EnsemblPlantsi | AT5G65165.1; AT5G65165.1; AT5G65165 |
GeneIDi | 836640 |
Gramenei | AT5G65165.1; AT5G65165.1; AT5G65165 |
KEGGi | ath:AT5G65165 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278912 mRNA Translation: CAC19857.1 AB013395 Genomic DNA Translation: BAB11652.1 CP002688 Genomic DNA Translation: AED98011.1 |
RefSeqi | NP_680465.2, NM_148160.3 |
3D structure databases
SMRi | Q9FJP9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 21882, 4 interactors |
STRINGi | 3702.AT5G65165.1 |
Proteomic databases
PaxDbi | Q9FJP9 |
PRIDEi | Q9FJP9 |
ProteomicsDBi | 232778 |
Genome annotation databases
EnsemblPlantsi | AT5G65165.1; AT5G65165.1; AT5G65165 |
GeneIDi | 836640 |
Gramenei | AT5G65165.1; AT5G65165.1; AT5G65165 |
KEGGi | ath:AT5G65165 |
Organism-specific databases
Araporti | AT5G65165 |
TAIRi | locus:504954878, AT5G65165 |
Phylogenomic databases
eggNOGi | KOG3049, Eukaryota |
HOGENOMi | CLU_044838_0_3_1 |
InParanoidi | Q9FJP9 |
OMAi | YDQYASI |
OrthoDBi | 1264157at2759 |
PhylomeDBi | Q9FJP9 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER01006 |
Miscellaneous databases
PROi | PR:Q9FJP9 |
Gene expression databases
ExpressionAtlasi | Q9FJP9, baseline and differential |
Family and domain databases
Gene3Di | 1.10.1060.10, 1 hit 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR006058, 2Fe2S_fd_BS IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR012675, Beta-grasp_dom_sf IPR009051, Helical_ferredxn IPR004489, Succ_DH/fum_Rdtase_Fe-S IPR025192, Succ_DH/fum_Rdtase_N |
Pfami | View protein in Pfam PF13085, Fer2_3, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR00384, dhsB, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SDHB3_ARATH | |
Accessioni | Q9FJP9Primary (citable) accession number: Q9FJP9 Secondary accession number(s): Q9G3L8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families