PME59

Functionⁱ

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityⁱ

[(1→4)-α-D-galacturonosyl methyl ester]_(n)
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+ n
H₂O
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See the description of this molecule in ChEBI.
=
[(1→4)-α-D-galacturonosyl]_(n)
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Search chemical reactions in Rhea for this molecule.
+ n
H⁺
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See the description of this molecule in ChEBI.
+ n
methanol
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See the description of this molecule in ChEBI.
EC:
3.1.1.11
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- See the description of this EC number in ENZYME.
Source:
Rhea
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See the description of this reaction in Rhea.
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[(1→4)-α-D-galacturonosyl methyl ester]_(n)
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+
n
H₂O
=
[(1→4)-α-D-galacturonosyl]_(n)
zoom
+
n
H⁺
+
n
methanol
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Pathwayⁱ: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	298	Substrate; for pectinesterase activityBy similarity	1
Binding siteⁱ	328	Substrate; for pectinesterase activityBy similarity	1
Siteⁱ	350	Transition state stabilizerBy similarity	1
Active siteⁱ	351	Proton donor; for pectinesterase activityPROSITE-ProRule annotation	1
Active siteⁱ	372	Nucleophile; for pectinesterase activityPROSITE-ProRule annotation	1
Binding siteⁱ	440	Substrate; for pectinesterase activityBy similarity	1
Binding siteⁱ	442	Substrate; for pectinesterase activityBy similarity	1

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell wall modification Source: InterPro
pectin catabolic process Source: UniProtKB-UniPathway

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aspartyl esterase, Hydrolase
Biological process	Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCycⁱ	ARA:AT5G51490-MONOMER
UniPathwayⁱ	UPA00545;UER00823

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable pectinesterase/pectinesterase inhibitor 59 Including the following 2 domains: Pectinesterase inhibitor 59 Alternative name(s): Pectin methylesterase inhibitor 59 Pectinesterase 59 (EC:3.1.1.11) Short name: PE 59 Alternative name(s): Pectin methylesterase 59 Short name: AtPME59
Gene namesⁱ	Name:PME59 Synonyms:ARATH59 Ordered Locus Names:At5g51490 ORF Names:K17N15.4
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliopsida › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 5

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT5G51490
TAIRⁱ	locus:2153112, AT5G51490

Keywords - Cellular componentⁱ

Cell wall, Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 30	Sequence analysisAdd BLAST		30
ChainⁱPRO_0000370180	31 – 536	Probable pectinesterase/pectinesterase inhibitor 59Add BLAST		506

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	33	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	91	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	116	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	159	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	195	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	365 ↔ 385	By similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	Q9FHN5
PRIDEⁱ	Q9FHN5
ProteomicsDBⁱ	235047

Expressionⁱ

Tissue specificityⁱ

Expressed in siliques.1 Publication

Developmental stageⁱ

Expressed during late developmental phases of siliques.1 Publication

Gene expression databases

ExpressionAtlasⁱ	Q9FHN5, baseline and differential
Genevisibleⁱ	Q9FHN5, AT

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	3702.AT5G51490.1

STRINGⁱ

3702.AT5G51490.1

Structureⁱ

3D structure databases

SMRⁱ	Q9FHN5
ModBaseⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	31 – 183	Pectinesterase inhibitor 59Add BLAST		153
Regionⁱ	221 – 522	Pectinesterase 59Add BLAST		302

Sequence similaritiesⁱ

In the N-terminal section; belongs to the PMEI family.Curated

In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG502QU67, Eukaryota
HOGENOMⁱ	CLU_012243_9_1_1
InParanoidⁱ	Q9FHN5
OMAⁱ	LNNDNLM
Database of Orthologous Groups More...OrthoDBⁱ	674407at2759
PhylomeDBⁱ	Q9FHN5

Family and domain databases

Gene3Dⁱ	1.20.140.40, 1 hit 2.160.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit
SMARTⁱ	View protein in SMART SM00856, PMEI, 1 hit
SUPFAMⁱ	SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit
TIGRFAMsⁱ	TIGR01614, PME_inhib, 1 hit
PROSITEⁱ	View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9FHN5-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MNMMMQKLSI LFLHLILLVL LCVHPLTTVA DRNSTDWCDK TPYPDPCKCY 
        60         70         80         90        100
FKNHNGFQQP TQLSEFRVML VEAAMDRAIS ARAELTNSGK NCTDSKKQAV 
       110        120        130        140        150
LADCIDLYGD TIMQLNRTLH GVSPKAGAAK SCTDFDAQTW LSTALTNTET 
       160        170        180        190        200
CRRGSSDLNV TDFITPIVSN TKISHLISNC LAVNGALLTA GNKGNTTANQ 
       210        220        230        240        250
KGFPTWLSRK DKRLLRAVRA NLVVAKDGSG HFNTVQAAID VAGRRKVTSG 
       260        270        280        290        300
RFVIYVKRGI YQENINVRLN NDDIMLVGDG MRSTIITGGR SVQGGYTTYN 
       310        320        330        340        350
SATAGIEGLH FIAKGITFRN TAGPAKGQAV ALRSSSDLSI FYKCSIEGYQ 
       360        370        380        390        400
DTLMVHSQRQ FYRECYIYGT VDFIFGNAAA VFQNCLILPR RPLKGQANVI 
       410        420        430        440        450
TAQGRADPFQ NTGISIHNSR ILPAPDLKPV VGTVKTYMGR PWMKFSRTVV 
       460        470        480        490        500
LQTYLDNVVS PVGWSPWIEG SVFGLDTLFY AEYKNTGPAS STRWRVSWKG 
       510        520        530 
FHVLGRASDA SAFTVGKFIA GTAWLPRTGI PFTSGL

Length:536

Mass (Da):58,868

Last modified:March 1, 2001 - v1

Checksum:ⁱ17FBC0F027F35BF9

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB018109 Genomic DNA Translation: BAB08665.1 CP002688 Genomic DNA Translation: AED96090.1
NCBI Reference Sequences More...RefSeqⁱ	NP_199962.1, NM_124528.3

Genome annotation databases

EnsemblPlantsⁱ	AT5G51490.1; AT5G51490.1; AT5G51490
GeneIDⁱ	835223
Grameneⁱ	AT5G51490.1; AT5G51490.1; AT5G51490
KEGGⁱ	ath:AT5G51490

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9FHN5	Pectinesterase		ARATH		536	UniRef100_Q9FHN5

Protein	Similar proteins		Species	Score	Length	Source
Q9FHN5	Pectinesterase		ARATH		536	UniRef90_Q9FHN5
Pectinesterase		ARALL		539
Pectinesterase		ARATH		536
Pectinesterase		ARATH		505
+3

Protein	Similar proteins		Species	Score	Length	Source
Q9FHN5	Probable pectinesterase/pectinesterase inhibitor 60		ARATH		540	UniRef50_Q9FHN4
Uncharacterized protein		Kingdonia uniflora		499
Pectinesterase		CICAR		539
Pectinesterase		SESIN		525
Pectinesterase		CAPCH		537
+340

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB018109 Genomic DNA Translation: BAB08665.1 CP002688 Genomic DNA Translation: AED96090.1
RefSeqⁱ	NP_199962.1, NM_124528.3

3D structure databases

SMRⁱ	Q9FHN5
ModBaseⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	3702.AT5G51490.1

STRINGⁱ

3702.AT5G51490.1

Proteomic databases

PaxDbⁱ	Q9FHN5
PRIDEⁱ	Q9FHN5
ProteomicsDBⁱ	235047

Genome annotation databases

EnsemblPlantsⁱ	AT5G51490.1; AT5G51490.1; AT5G51490
GeneIDⁱ	835223
Grameneⁱ	AT5G51490.1; AT5G51490.1; AT5G51490
KEGGⁱ	ath:AT5G51490

Organism-specific databases

Araportⁱ	AT5G51490
TAIRⁱ	locus:2153112, AT5G51490

Phylogenomic databases

eggNOGⁱ	ENOG502QU67, Eukaryota
HOGENOMⁱ	CLU_012243_9_1_1
InParanoidⁱ	Q9FHN5
OMAⁱ	LNNDNLM
OrthoDBⁱ	674407at2759
PhylomeDBⁱ	Q9FHN5

Enzyme and pathway databases

UniPathwayⁱ	UPA00545;UER00823
BioCycⁱ	ARA:AT5G51490-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9FHN5

PROⁱ

PR:Q9FHN5

Gene expression databases

ExpressionAtlasⁱ	Q9FHN5, baseline and differential
Genevisibleⁱ	Q9FHN5, AT

Family and domain databases

Gene3Dⁱ	1.20.140.40, 1 hit 2.160.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS
Pfamⁱ	View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit
SMARTⁱ	View protein in SMART SM00856, PMEI, 1 hit
SUPFAMⁱ	SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit
TIGRFAMsⁱ	TIGR01614, PME_inhib, 1 hit
PROSITEⁱ	View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PME59_ARATH
Accessionⁱ	Q9FHN5Primary (citable) accession number: Q9FHN5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
	Last sequence update:	March 1, 2001
	Last modified:	December 2, 2020
	This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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UniProtKB - Q9FHN5 (PME59_ARATH)

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Probable pectinesterase/pectinesterase inhibitor 59

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: pectin degradation

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cell wall

Cell Wall

Extracellular region or secreted

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Catalytic activityⁱ

Pathwayⁱ: pectin degradation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ