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UniProtKB - Q9FHN5 (PME59_ARATH)
Protein
Probable pectinesterase/pectinesterase inhibitor 59
Gene
PME59
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Acts in the modification of cell walls via demethylesterification of cell wall pectin.
By similarityMiscellaneous
The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.
Catalytic activityi
- [(1→4)-α-D-galacturonosyl methyl ester](n) + n H2O = [(1→4)-α-D-galacturonosyl](n) + n H+ + n methanolEC:3.1.1.11
: pectin degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin. This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 298 | Substrate; for pectinesterase activityBy similarity | 1 | |
Binding sitei | 328 | Substrate; for pectinesterase activityBy similarity | 1 | |
Sitei | 350 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 351 | Proton donor; for pectinesterase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 372 | Nucleophile; for pectinesterase activityPROSITE-ProRule annotation | 1 | |
Binding sitei | 440 | Substrate; for pectinesterase activityBy similarity | 1 | |
Binding sitei | 442 | Substrate; for pectinesterase activityBy similarity | 1 |
GO - Molecular functioni
- aspartyl esterase activity Source: UniProtKB-KW
- pectinesterase activity Source: GO_Central
- pectinesterase inhibitor activity Source: GO_Central
GO - Biological processi
- cell wall modification Source: InterPro
- pectin catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Aspartyl esterase, Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | ARA:AT5G51490-MONOMER |
UniPathwayi | UPA00545;UER00823 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable pectinesterase/pectinesterase inhibitor 59Including the following 2 domains: Pectinesterase inhibitor 59 Alternative name(s): Pectin methylesterase inhibitor 59 Alternative name(s): Pectin methylesterase 59 Short name: AtPME59 |
Gene namesi | Name:PME59 Synonyms:ARATH59 Ordered Locus Names:At5g51490 ORF Names:K17N15.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G51490 |
TAIRi | locus:2153112, AT5G51490 |
Subcellular locationi
Cell wall
- cell wall By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Keywords - Cellular componenti
Cell wall, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 30 | Sequence analysisAdd BLAST | 30 | |
ChainiPRO_0000370180 | 31 – 536 | Probable pectinesterase/pectinesterase inhibitor 59Add BLAST | 506 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 33 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 91 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 116 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 159 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 195 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 365 ↔ 385 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9FHN5 |
PRIDEi | Q9FHN5 |
ProteomicsDBi | 235047 |
Expressioni
Tissue specificityi
Expressed in siliques.1 Publication
Developmental stagei
Expressed during late developmental phases of siliques.1 Publication
Gene expression databases
ExpressionAtlasi | Q9FHN5, baseline and differential |
Genevisiblei | Q9FHN5, AT |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 31 – 183 | Pectinesterase inhibitor 59Add BLAST | 153 | |
Regioni | 221 – 522 | Pectinesterase 59Add BLAST | 302 |
Sequence similaritiesi
In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QU67, Eukaryota |
HOGENOMi | CLU_012243_9_1_1 |
InParanoidi | Q9FHN5 |
OMAi | GLFVIYV |
OrthoDBi | 674407at2759 |
PhylomeDBi | Q9FHN5 |
Family and domain databases
Gene3Di | 1.20.140.40, 1 hit 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit |
SMARTi | View protein in SMART SM00856, PMEI, 1 hit |
SUPFAMi | SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit |
TIGRFAMsi | TIGR01614, PME_inhib, 1 hit |
PROSITEi | View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9FHN5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNMMMQKLSI LFLHLILLVL LCVHPLTTVA DRNSTDWCDK TPYPDPCKCY
60 70 80 90 100
FKNHNGFQQP TQLSEFRVML VEAAMDRAIS ARAELTNSGK NCTDSKKQAV
110 120 130 140 150
LADCIDLYGD TIMQLNRTLH GVSPKAGAAK SCTDFDAQTW LSTALTNTET
160 170 180 190 200
CRRGSSDLNV TDFITPIVSN TKISHLISNC LAVNGALLTA GNKGNTTANQ
210 220 230 240 250
KGFPTWLSRK DKRLLRAVRA NLVVAKDGSG HFNTVQAAID VAGRRKVTSG
260 270 280 290 300
RFVIYVKRGI YQENINVRLN NDDIMLVGDG MRSTIITGGR SVQGGYTTYN
310 320 330 340 350
SATAGIEGLH FIAKGITFRN TAGPAKGQAV ALRSSSDLSI FYKCSIEGYQ
360 370 380 390 400
DTLMVHSQRQ FYRECYIYGT VDFIFGNAAA VFQNCLILPR RPLKGQANVI
410 420 430 440 450
TAQGRADPFQ NTGISIHNSR ILPAPDLKPV VGTVKTYMGR PWMKFSRTVV
460 470 480 490 500
LQTYLDNVVS PVGWSPWIEG SVFGLDTLFY AEYKNTGPAS STRWRVSWKG
510 520 530
FHVLGRASDA SAFTVGKFIA GTAWLPRTGI PFTSGL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB018109 Genomic DNA Translation: BAB08665.1 CP002688 Genomic DNA Translation: AED96090.1 |
RefSeqi | NP_199962.1, NM_124528.3 |
Genome annotation databases
EnsemblPlantsi | AT5G51490.1; AT5G51490.1; AT5G51490 |
GeneIDi | 835223 |
Gramenei | AT5G51490.1; AT5G51490.1; AT5G51490 |
KEGGi | ath:AT5G51490 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB018109 Genomic DNA Translation: BAB08665.1 CP002688 Genomic DNA Translation: AED96090.1 |
RefSeqi | NP_199962.1, NM_124528.3 |
3D structure databases
SMRi | Q9FHN5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G51490.1 |
Proteomic databases
PaxDbi | Q9FHN5 |
PRIDEi | Q9FHN5 |
ProteomicsDBi | 235047 |
Genome annotation databases
EnsemblPlantsi | AT5G51490.1; AT5G51490.1; AT5G51490 |
GeneIDi | 835223 |
Gramenei | AT5G51490.1; AT5G51490.1; AT5G51490 |
KEGGi | ath:AT5G51490 |
Organism-specific databases
Araporti | AT5G51490 |
TAIRi | locus:2153112, AT5G51490 |
Phylogenomic databases
eggNOGi | ENOG502QU67, Eukaryota |
HOGENOMi | CLU_012243_9_1_1 |
InParanoidi | Q9FHN5 |
OMAi | GLFVIYV |
OrthoDBi | 674407at2759 |
PhylomeDBi | Q9FHN5 |
Enzyme and pathway databases
UniPathwayi | UPA00545;UER00823 |
BioCyci | ARA:AT5G51490-MONOMER |
Miscellaneous databases
PROi | PR:Q9FHN5 |
Gene expression databases
ExpressionAtlasi | Q9FHN5, baseline and differential |
Genevisiblei | Q9FHN5, AT |
Family and domain databases
Gene3Di | 1.20.140.40, 1 hit 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR035513, Invertase/methylesterase_inhib IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat IPR006501, Pectinesterase_inhib_dom IPR018040, Pectinesterase_Tyr_AS |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit PF04043, PMEI, 1 hit |
SMARTi | View protein in SMART SM00856, PMEI, 1 hit |
SUPFAMi | SSF101148, SSF101148, 1 hit SSF51126, SSF51126, 1 hit |
TIGRFAMsi | TIGR01614, PME_inhib, 1 hit |
PROSITEi | View protein in PROSITE PS00800, PECTINESTERASE_1, 1 hit PS00503, PECTINESTERASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PME59_ARATH | |
Accessioni | Q9FHN5Primary (citable) accession number: Q9FHN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 14, 2009 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families