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Protein

Abscisic acid 8'-hydroxylase 3

Gene

CYP707A3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of postgermination growth.3 Publications

Catalytic activityi

+-abscisate + [reduced NADPH--hemoprotein reductase] + O2 = 8'-hydroxyabscisate + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Activity regulationi

Inhibited by tetcyclcis, but not by metyrapone.1 Publication

Kineticsi

  1. KM=1.3 µM for (+)-ABA1 Publication

    Pathwayi: abscisic acid degradation

    This protein is involved in the pathway abscisic acid degradation, which is part of Plant hormone degradation.
    View all proteins of this organism that are known to be involved in the pathway abscisic acid degradation and in Plant hormone degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi411Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    • (+)-abscisic acid 8'-hydroxylase activity Source: TAIR
    • heme binding Source: InterPro
    • iron ion binding Source: InterPro
    • monooxygenase activity Source: GO_Central

    GO - Biological processi

    • abscisic acid catabolic process Source: TAIR
    • abscisic acid metabolic process Source: GO_Central
    • oxidation-reduction process Source: GO_Central
    • response to red or far red light Source: TAIR
    • response to water deprivation Source: TAIR
    • sterol metabolic process Source: GO_Central

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    Biological processStress response
    LigandHeme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G45340-MONOMER
    MetaCyc:AT5G45340-MONOMER
    BRENDAi1.14.13.93 399
    ReactomeiR-ATH-211916 Vitamins
    R-ATH-5365859 RA biosynthesis pathway
    SABIO-RKiQ9FH76
    UniPathwayi
    UPA00093

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Abscisic acid 8'-hydroxylase 3 (EC:1.14.14.137By similarity)
    Short name:
    ABA 8'-hydroxylase 3
    Alternative name(s):
    Cytochrome P450 707A3
    Gene namesi
    Name:CYP707A3
    Ordered Locus Names:At5g45340
    ORF Names:K9E15.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G45340
    TAIRilocus:2158480 AT5G45340

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei6 – 26HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Plants are not affected in seed germination, but show a restricted greening rate after germination and increase the drought resistance.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi411C → A: Loss of activity. 1 Publication1

    Chemistry databases

    ChEMBLiCHEMBL4538

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002886411 – 463Abscisic acid 8'-hydroxylase 3Add BLAST463

    Proteomic databases

    PaxDbiQ9FH76

    PTM databases

    iPTMnetiQ9FH76

    Expressioni

    Tissue specificityi

    Mainly expressed in flower buds, flowers, rosette leaves and roots. Lower expression in mature siliques and inflorescence stems. Not expressed in dry seeds.2 Publications

    Developmental stagei

    Up-regulated 12 hours after imbibition.1 Publication

    Inductioni

    By abscisic acid, brassinosteroid or gibberellin treatments, by salt or osmotic stresses, and by dehydration and rehydration. Expression regulated by phytochrome B.4 Publications

    Gene expression databases

    ExpressionAtlasiQ9FH76 baseline and differential
    GenevisibleiQ9FH76 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G45340.1

    Chemistry databases

    BindingDBiQ9FH76

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FH76
    SMRiQ9FH76
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0157 Eukaryota
    COG2124 LUCA
    HOGENOMiHOG000237614
    KOiK09843
    OMAiPHVCPGA
    OrthoDBiEOG0936099G
    PhylomeDBiQ9FH76

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
    Isoform 1 (identifier: Q9FH76-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MDFSGLFLTL SAAALFLCLL RFIAGVRRSS STKLPLPPGT MGYPYVGETF
    60 70 80 90 100
    QLYSQDPNVF FAAKQRRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL
    110 120 130 140 150
    FKPTFPASKE RMLGKQAIFF HQGDYHSKLR KLVLRAFMPD AIRNMVPHIE
    160 170 180 190 200
    SIAQESLNSW DGTQLNTYQE MKTYTFNVAL ISILGKDEVY YREDLKRCYY
    210 220 230 240 250
    ILEKGYNSMP INLPGTLFHK AMKARKELAQ ILANILSKRR QNPSSHTDLL
    260 270 280 290 300
    GSFMEDKAGL TDEQIADNII GVIFAARDTT ASVLTWILKY LADNPTVLEA
    310 320 330 340 350
    VTEEQMAIRK DKKEGESLTW EDTKKMPLTY RVIQETLRAA TILSFTFREA
    360 370 380 390 400
    VEDVEYEGYL IPKGWKVLPL FRNIHHNADI FSDPGKFDPS RFEVAPKPNT
    410 420 430 440 450
    FMPFGSGIHS CPGNELAKLE ISVLIHHLTT KYRWSIVGPS DGIQYGPFAL
    460
    PQNGLPIALE RKP
    Length:463
    Mass (Da):52,367
    Last modified:March 1, 2001 - v1
    Checksum:iCCD17293F553F812
    GO
    Isoform 2 (identifier: Q9FH76-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         434-447: WSIVGPSDGIQYGP → LVHLQNDNSPFGN
         448-463: Missing.

    Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
    Show »
    Length:446
    Mass (Da):50,599
    Checksum:i281DA0613C096FD3
    GO

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_025739434 – 447WSIVG…IQYGP → LVHLQNDNSPFGN in isoform 2. CuratedAdd BLAST14
    Alternative sequenceiVSP_025740448 – 463Missing in isoform 2. CuratedAdd BLAST16

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB122150 mRNA Translation: BAD16630.1
    AB020744 Genomic DNA Translation: BAB10255.1
    CP002688 Genomic DNA Translation: AED95234.1
    CP002688 Genomic DNA Translation: AED95235.1
    AY065065 mRNA Translation: AAL57698.1
    AY102136 mRNA Translation: AAM26703.1
    RefSeqiNP_199347.2, NM_123902.3 [Q9FH76-2]
    NP_851136.1, NM_180805.4 [Q9FH76-1]
    UniGeneiAt.9625

    Genome annotation databases

    EnsemblPlantsiAT5G45340.1; AT5G45340.1; AT5G45340 [Q9FH76-1]
    AT5G45340.2; AT5G45340.2; AT5G45340 [Q9FH76-2]
    GeneIDi834570
    GrameneiAT5G45340.1; AT5G45340.1; AT5G45340 [Q9FH76-1]
    AT5G45340.2; AT5G45340.2; AT5G45340 [Q9FH76-2]
    KEGGiath:AT5G45340

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB122150 mRNA Translation: BAD16630.1
    AB020744 Genomic DNA Translation: BAB10255.1
    CP002688 Genomic DNA Translation: AED95234.1
    CP002688 Genomic DNA Translation: AED95235.1
    AY065065 mRNA Translation: AAL57698.1
    AY102136 mRNA Translation: AAM26703.1
    RefSeqiNP_199347.2, NM_123902.3 [Q9FH76-2]
    NP_851136.1, NM_180805.4 [Q9FH76-1]
    UniGeneiAt.9625

    3D structure databases

    ProteinModelPortaliQ9FH76
    SMRiQ9FH76
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT5G45340.1

    Chemistry databases

    BindingDBiQ9FH76
    ChEMBLiCHEMBL4538

    PTM databases

    iPTMnetiQ9FH76

    Proteomic databases

    PaxDbiQ9FH76

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G45340.1; AT5G45340.1; AT5G45340 [Q9FH76-1]
    AT5G45340.2; AT5G45340.2; AT5G45340 [Q9FH76-2]
    GeneIDi834570
    GrameneiAT5G45340.1; AT5G45340.1; AT5G45340 [Q9FH76-1]
    AT5G45340.2; AT5G45340.2; AT5G45340 [Q9FH76-2]
    KEGGiath:AT5G45340

    Organism-specific databases

    AraportiAT5G45340
    TAIRilocus:2158480 AT5G45340

    Phylogenomic databases

    eggNOGiKOG0157 Eukaryota
    COG2124 LUCA
    HOGENOMiHOG000237614
    KOiK09843
    OMAiPHVCPGA
    OrthoDBiEOG0936099G
    PhylomeDBiQ9FH76

    Enzyme and pathway databases

    UniPathwayi
    UPA00093

    BioCyciARA:AT5G45340-MONOMER
    MetaCyc:AT5G45340-MONOMER
    BRENDAi1.14.13.93 399
    ReactomeiR-ATH-211916 Vitamins
    R-ATH-5365859 RA biosynthesis pathway
    SABIO-RKiQ9FH76

    Miscellaneous databases

    PROiPR:Q9FH76

    Gene expression databases

    ExpressionAtlasiQ9FH76 baseline and differential
    GenevisibleiQ9FH76 AT

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiABAH3_ARATH
    AccessioniPrimary (citable) accession number: Q9FH76
    Secondary accession number(s): Q3E8G7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: March 1, 2001
    Last modified: November 7, 2018
    This is version 131 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
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    Main funding by: National Institutes of Health

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