UniProtKB - Q9FGI6 (NDUS1_ARATH)
Protein
NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial
Gene
EMB1467
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity
Catalytic activityi
- EC:7.1.1.2
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 106 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 117 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 120 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 134 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 166 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 170 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 173 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 179 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 218 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 221 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 224 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 268 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
- photorespiration Source: TAIR
- response to oxidative stress Source: TAIR
Keywordsi
Molecular function | Oxidoreductase, Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | ARA:AT5G37510-MONOMER MetaCyc:AT5G37510-MONOMER |
Protein family/group databases
TCDBi | 3.D.1.6.3, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Names & Taxonomyi
Protein namesi | Recommended name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (EC:7.1.1.2)Alternative name(s): Protein EMBRYO DEFECTIVE 1467 |
Gene namesi | Name:EMB1467 Ordered Locus Names:At5g37510 ORF Names:MPA22.5 |
Organismi | Arabidopsis thaliana (Mouse-ear cress)Imported |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G37510 |
TAIRi | locus:2169871, AT5G37510 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity
Chloroplast
- chloroplast Source: TAIR
Mitochondrion
- mitochondrial membrane Source: TAIR
- mitochondrial respiratory chain complex I Source: TAIR
- mitochondrion Source: TAIR
Other locations
- plastid Source: TAIR
- respiratory chain complex I Source: TAIR
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 33 | MitochondrionBy similarityAdd BLAST | 33 | |
ChainiPRO_0000019972 | 34 – 748 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrialAdd BLAST | 715 |
Proteomic databases
PaxDbi | Q9FGI6 |
PRIDEi | Q9FGI6 |
ProteomicsDBi | 251103 [Q9FGI6-1] |
PTM databases
iPTMneti | Q9FGI6 |
MetOSitei | Q9FGI6 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9FGI6, baseline and differential |
Genevisiblei | Q9FGI6, AT |
Interactioni
Subunit structurei
Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.
Protein-protein interaction databases
BioGRIDi | 18980, 3 interactors |
IntActi | Q9FGI6, 2 interactors |
STRINGi | 3702.AT5G37510.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 72 – 150 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 150 – 189 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 287 – 343 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2282, Eukaryota |
InParanoidi | Q9FGI6 |
OrthoDBi | 1095510at2759 |
PhylomeDBi | Q9FGI6 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405, NuoG_C |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit PF09326, NADH_dhqG_C, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9FGI6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGLGILASRT IRPASRLLQS QTSNFFLRTI VSKPELQSPE SAAVSEPEPP
60 70 80 90 100
TQILPPRNPV GGARVHFSNP EDAIEVFVDG YAVKVPKGFT VLQACEVAGV
110 120 130 140 150
DIPRFCYHSR LSIAGNCRMC LVEVEKSPKP VASCAMPALP GMKIKTDTPI
160 170 180 190 200
AKKAREGVME FLLMNHPLDC PICDQGGECD LQDQSMAFGS DRGRFTEMKR
210 220 230 240 250
SVVDKNLGPL VKTVMTRCIQ CTRCVRFASE VAGVQDLGIL GRGSGEEIGT
260 270 280 290 300
YVEKLMTSEL SGNVIDICPV GALTSKPFAF KARNWELKAT ETIDVSDAVG
310 320 330 340 350
SNIRVDSRGP EVMRIIPRLN EDINEEWISD KTRFCYDGLK RQRLSDPMIR
360 370 380 390 400
DSDGRFKAVS WRDALAVVGD IIHQVKPDEI VGVAGQLSDA ESMMVLKDFV
410 420 430 440 450
NRMGSDNVWC EGTAAGVDAD LRYSYLMNTS ISGLENADLF LLIGTQPRVE
460 470 480 490 500
AAMVNARICK TVRASNAKVG YVGPPAEFNY DCKHLGTGPD TLKEIAEGRH
510 520 530 540 550
PFCTALKNAK NPAIIVGAGL FNRTDKNAIL SSVESIAQAN NVVRPDWNGL
560 570 580 590 600
NFLLQYAAQA AALDLGLIQQ SAKALESAKF VYLMGADDVN VDKIPKDAFV
610 620 630 640 650
VYQGHHGDKA VYRANVILPA SAFTEKEGTY ENTEGFTQQT VPAVPTVGDA
660 670 680 690 700
RDDWKIVRAL SEVSGVKLPY NSIEGVRSRI KSVAPNLVHT DEREPAAFGP
710 720 730 740
SLKPECKEAM STTPFQTVVE NFYMTNSITR ASKIMAQCSA VLLKKPFV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB9DFQ9 | B9DFQ9_ARATH | AT5G37510 protein | EMB1467 CI76, embryo defective 1467, MPA22.5, MPA22_5, At5g37510 | 745 | Annotation score: |
Sequence cautioni
The sequence BAB10668 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025630 Genomic DNA Translation: BAB10668.1 Sequence problems. CP002688 Genomic DNA Translation: AED94200.1 AY056140 mRNA Translation: AAL07219.1 |
RefSeqi | NP_568550.1, NM_123110.2 [Q9FGI6-1] NP_851103.1, NM_180772.2 |
Genome annotation databases
EnsemblPlantsi | AT5G37510.2; AT5G37510.2; AT5G37510 [Q9FGI6-1] |
GeneIDi | 833729 |
Gramenei | AT5G37510.2; AT5G37510.2; AT5G37510 [Q9FGI6-1] |
KEGGi | ath:AT5G37510 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025630 Genomic DNA Translation: BAB10668.1 Sequence problems. CP002688 Genomic DNA Translation: AED94200.1 AY056140 mRNA Translation: AAL07219.1 |
RefSeqi | NP_568550.1, NM_123110.2 [Q9FGI6-1] NP_851103.1, NM_180772.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7A23 | electron microscopy | 3.70 | C | 1-748 | [»] | |
7A24 | electron microscopy | 3.80 | C | 1-748 | [»] | |
SMRi | Q9FGI6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 18980, 3 interactors |
IntActi | Q9FGI6, 2 interactors |
STRINGi | 3702.AT5G37510.2 |
Protein family/group databases
TCDBi | 3.D.1.6.3, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
PTM databases
iPTMneti | Q9FGI6 |
MetOSitei | Q9FGI6 |
Proteomic databases
PaxDbi | Q9FGI6 |
PRIDEi | Q9FGI6 |
ProteomicsDBi | 251103 [Q9FGI6-1] |
Genome annotation databases
EnsemblPlantsi | AT5G37510.2; AT5G37510.2; AT5G37510 [Q9FGI6-1] |
GeneIDi | 833729 |
Gramenei | AT5G37510.2; AT5G37510.2; AT5G37510 [Q9FGI6-1] |
KEGGi | ath:AT5G37510 |
Organism-specific databases
Araporti | AT5G37510 |
TAIRi | locus:2169871, AT5G37510 |
Phylogenomic databases
eggNOGi | KOG2282, Eukaryota |
InParanoidi | Q9FGI6 |
OrthoDBi | 1095510at2759 |
PhylomeDBi | Q9FGI6 |
Enzyme and pathway databases
BioCyci | ARA:AT5G37510-MONOMER MetaCyc:AT5G37510-MONOMER |
Miscellaneous databases
PROi | PR:Q9FGI6 |
Gene expression databases
ExpressionAtlasi | Q9FGI6, baseline and differential |
Genevisiblei | Q9FGI6, AT |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405, NuoG_C |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit PF09326, NADH_dhqG_C, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDUS1_ARATH | |
Accessioni | Q9FGI6Primary (citable) accession number: Q9FGI6 Secondary accession number(s): Q940B1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 15, 2002 |
Last sequence update: | October 25, 2004 | |
Last modified: | April 7, 2021 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families