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Entry version 134 (02 Jun 2021)
Sequence version 1 (01 Mar 2001)
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Protein

Ubiquitin carboxyl-terminal hydrolase

Gene

UCH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Ubiquitin-protein hydrolase involved in the release of ubiquitin attached via both peptide and isopeptide linkages. Able to cleave 'Lys-48'-linked polyubiquitin chains. Involved in the direct or indirect regulation of AUX/IAA proteins stability (Probable). Acts as a linker between the TREX-2 complex and 26S proteasome (PubMed:22951400).

1 Publication1 Publication

Miscellaneous

UCH1 and UCH2 are not integral polypeptides of the 26S proteasome, unlike their S.pombe and animal orthologs (PubMed:17559514). However, they interact with the 26S proteasome lid complex as well as to the TREX-2 complex (PubMed:22951400).2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).Curated EC:3.4.19.12

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei82NucleophileBy similarity1
Active sitei163Proton donorBy similarity1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei178Important for enzyme activityBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPS protease database

More...
MEROPSi
C12.A01

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase1 Publication (EC:3.4.19.12Curated)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:UCH11 Publication
Ordered Locus Names:At5g16310Imported
ORF Names:MQK4.3Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

Organism-specific databases

Arabidopsis Information Portal

More...
Araporti
AT5G16310

The Arabidopsis Information Resource

More...
TAIRi
locus:2171312, AT5G16310

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

No visible phenotype. Uch1 and uch2 double mutants are less fertile, accumulated less chlorophyll and have slightly fewer and shorter cauline branches.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004354061 – 334Ubiquitin carboxyl-terminal hydrolaseAdd BLAST334

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9FFF2

PRoteomics IDEntifications database

More...
PRIDEi
Q9FFF2

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
243219

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in leaves, stems, sepals, stamens, petals, roots, hypocotyls and cotyledons.1 Publication

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9FFF2, baseline and differential

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer (PubMed:22951400).

Interacts with EER5 (PubMed:19843313, PubMed:22951400).

Interacts with UCH2, DSS1(V), DSS1(I), RPN3A, RPN3B, RPN12A, RPN12B, SAC3B, CML20 and NUP1 (PubMed:22951400).

2 Publications

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
Q9FFF2, 3 interactors

STRING: functional protein association networks

More...
STRINGi
3702.AT5G16310.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9FFF2

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2778, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_018316_0_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q9FFF2

Identification of Orthologs from Complete Genome Data

More...
OMAi
ALQADKY

Database of Orthologous Groups

More...
OrthoDBi
1363547at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9FFF2

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.532.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR038765, Papain-like_cys_pep_sf
IPR001578, Peptidase_C12_UCH
IPR036959, Peptidase_C12_UCH_sf
IPR017390, Ubiquitinyl_hydrolase_UCH37
IPR041507, UCH_C

The PANTHER Classification System

More...
PANTHERi
PTHR10589, PTHR10589, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01088, Peptidase_C12, 1 hit
PF18031, UCH_C, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF038120, Ubiquitinyl_hydrolase_UCH37, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00707, UBCTHYDRLASE

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF54001, SSF54001, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9FFF2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSWLPVESDP GIFTEIIQQM QVKGVQVEEL YSLDFNSLDE IRPVYGLILL
60 70 80 90 100
YKWRPEEKEN RVVITEPNPN FFFASQIINN ACATQAILSV LMNSSSIDIG
110 120 130 140 150
SELSELKQFA KEFPPELKGL AINNNEAIRA AHNTFARPDP SSIMEDEELA
160 170 180 190 200
AAKNLDEDDD VYHYISYLPV DGILYELDGL KEGPISLGQC LGEPEGIEWL
210 220 230 240 250
RMVQPVVQEQ IDRYSQNEIR FSLLAVVKNR KEMYVAELKE YQRKRERVLQ
260 270 280 290 300
QLGALQADKY AEKSSYEALD RELSEVNIGI ETVSQKIVME EEKSKNWKKE
310 320 330
NMRRKHNYVP FLFNFLKILA DKKKLKPLIA KHHP
Length:334
Mass (Da):38,539
Last modified:March 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE47D938864E44BED
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB005242 Genomic DNA Translation: BAB09598.1
CP002688 Genomic DNA Translation: AED92278.1
AK118002 mRNA Translation: BAC42635.1
BT005312 mRNA Translation: AAO63376.1

NCBI Reference Sequences

More...
RefSeqi
NP_197135.1, NM_121636.3

Genome annotation databases

Ensembl plant genome annotation project

More...
EnsemblPlantsi
AT5G16310.1; AT5G16310.1; AT5G16310

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
831492

Gramene; a comparative resource for plants

More...
Gramenei
AT5G16310.1; AT5G16310.1; AT5G16310

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ath:AT5G16310

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005242 Genomic DNA Translation: BAB09598.1
CP002688 Genomic DNA Translation: AED92278.1
AK118002 mRNA Translation: BAC42635.1
BT005312 mRNA Translation: AAO63376.1
RefSeqiNP_197135.1, NM_121636.3

3D structure databases

SMRiQ9FFF2
ModBaseiSearch...

Protein-protein interaction databases

IntActiQ9FFF2, 3 interactors
STRINGi3702.AT5G16310.1

Protein family/group databases

MEROPSiC12.A01

Proteomic databases

PaxDbiQ9FFF2
PRIDEiQ9FFF2
ProteomicsDBi243219

Genome annotation databases

EnsemblPlantsiAT5G16310.1; AT5G16310.1; AT5G16310
GeneIDi831492
GrameneiAT5G16310.1; AT5G16310.1; AT5G16310
KEGGiath:AT5G16310

Organism-specific databases

AraportiAT5G16310
TAIRilocus:2171312, AT5G16310

Phylogenomic databases

eggNOGiKOG2778, Eukaryota
HOGENOMiCLU_018316_0_1_1
InParanoidiQ9FFF2
OMAiALQADKY
OrthoDBi1363547at2759
PhylomeDBiQ9FFF2

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q9FFF2

Gene expression databases

ExpressionAtlasiQ9FFF2, baseline and differential

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR038765, Papain-like_cys_pep_sf
IPR001578, Peptidase_C12_UCH
IPR036959, Peptidase_C12_UCH_sf
IPR017390, Ubiquitinyl_hydrolase_UCH37
IPR041507, UCH_C
PANTHERiPTHR10589, PTHR10589, 1 hit
PfamiView protein in Pfam
PF01088, Peptidase_C12, 1 hit
PF18031, UCH_C, 1 hit
PIRSFiPIRSF038120, Ubiquitinyl_hydrolase_UCH37, 1 hit
PRINTSiPR00707, UBCTHYDRLASE
SUPFAMiSSF54001, SSF54001, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiUCH1_ARATH
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9FFF2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: March 1, 2001
Last modified: June 2, 2021
This is version 134 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
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