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Protein

Probable pectinesterase/pectinesterase inhibitor 47

Gene

PME47

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase PPME1 (PPME1), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Putative pectinesterase 11 (PME11), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase 67 (PME67), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 49 (PME49), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Probable pectinesterase 50 (PME50), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Pectinesterase QRT1 (QRT1), Putative pectinesterase 63 (PME63), Probable pectinesterase 30 (PME30), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Pectinesterase/pectinesterase inhibitor 18 (PME18), Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase 48 (PME48), Pectinesterase 4 (PME4), Putative pectinesterase 14 (PME14), Probable pectinesterase 55 (PME55), Probable pectinesterase 56 (PME56), Probable pectinesterase 66 (PME66), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase 8 (PME8), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase/pectinesterase inhibitor 3 (PME3), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Pectinesterase 1 (PME1), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Putative pectinesterase 57 (PME57), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase 5 (PME5), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 29 (PME29), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Uncharacterized protein (AXX17_At3g05180), Pectinesterase (AXX17_At5g52320), Pectinesterase (AXX17_At3g17940), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At5g50300), PME26 (AXX17_At3g14890), Pectinesterase (AXX17_At4g02940), Pectinesterase (At4g15980), Pectinesterase (At3g14310), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (At5g26810), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g45340), Pectinesterase (At3g10720), Pectinesterase (AXX17_At5g04430), Pectinesterase (AXX17_At1g11890), Uncharacterized protein (AXX17_At5g27870), Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At5g50310), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At3g06710), Pectinesterase (AXX17_At3g55110), Pectinesterase (At5g20860), Pectinesterase (AXX17_At4g38040), Pectinesterase (AXX17_At1g04730), Pectinesterase (AXX17_At1g11900), Pectinesterase (AXX17_At2g33410), Pectinesterase, Pectinesterase (AXX17_At2g22270), Pectinesterase (AXX17_At2g40510), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g18720), Pectinesterase (AXX17_At4g02930), Pectinesterase (AXX17_At5g47900), Pectinesterase (AXX17_At4g38050), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At1g11920), Pectinesterase (At3g49220), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g19650), Pectinesterase, Pectinesterase (AXX17_At3g05190), Pectinesterase (AXX17_At3g30550), Pectinesterase (AXX17_At4g06570), Pectinesterase (At1g53840), Pectinesterase, Pectinesterase (AXX17_At4g02900), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At5g45990), Pectinesterase (AXX17_At2g44790), Pectinesterase (F14I3.7), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At3g26070), Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At3g10570), Pectinesterase (AXX17_At3g43380), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (PME44), Pectinesterase (PMEPCRF), Pectinesterase (AXX17_At1g48290), Pectinesterase, Pectinesterase (AXX17_At2g22280)
  2. Probable pectate lyase 10 (At3g24670), Putative pectate lyase 11 (At3g27400), Probable pectate lyase 15 (At4g13710), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 1 (At1g04680), Putative pectate lyase 14 (At4g13210), Probable pectate lyase 19 (At5g15110), Putative pectate lyase 21 (At5g55720), Probable pectate lyase 22 (At5g63180), Putative pectate lyase 2 (At1g11920), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 6 (At2g02720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Probable pectate lyase 13 (PMR6), Probable pectate lyase 12 (At3g53190), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 9 (At3g24230), Pectate lyase (At3g01270), Pectate lyase (At3g09540), Pectate lyase (AXX17_At3g09180), Pectate lyase (At5g09280), Pectate lyase (At5g04310), Pectate lyase (F11F8_12), Pectate lyase (At1g14420), Pectate lyase (At3g55140), Pectate lyase (AXX17_At4g28670), Pectate lyase, Pectate lyase (At3g27400), Pectate lyase (AXX17_At5g03700), Pectate lyase (AXX17_At4g25610), Pectate lyase (AXX17_At3g00310), Pectate lyase (AXX17_At3g49720), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g06910), Pectate lyase (AXX17_At3g47590), Pectate lyase (At5g04310), Pectate lyase (At4g13710), Pectate lyase, Pectate lyase (At3g53190), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At5g08800), Pectate lyase (At4g13210), Pectate lyase (At3g55140), Pectate lyase (At3g01270), Pectate lyase (AXX17_At4g15660), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At2g01680), Pectate lyase (AXX17_At3g26720), Pectate lyase (AXX17_At5g14600), Pectate lyase (AXX17_At1g04020), Pectate lyase (AXX17_At1g12300), Pectate lyase (At3g07010), Pectate lyase (AXX17_At1g15010), Pectate lyase (AXX17_At1g30780), Pectate lyase (AXX17_At5g47570), Pectate lyase, Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At1g61710), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g26200), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g62760), Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei385Substrate; for pectinesterase activityBy similarity1
Binding sitei415Substrate; for pectinesterase activityBy similarity1
Sitei437Transition state stabilizerBy similarity1
Active sitei438Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei459Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei527Substrate; for pectinesterase activityBy similarity1
Binding sitei529Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G04970-MONOMER
UniPathwayi
UPA00545;UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 47
Including the following 2 domains:
Pectinesterase inhibitor 47
Alternative name(s):
Pectin methylesterase inhibitor 47
Pectinesterase 47 (EC:3.1.1.11)
Short name:
PE 47
Alternative name(s):
Pectin methylesterase 47
Short name:
AtPME47
Gene namesi
Name:PME47
Synonyms:ARATH47
Ordered Locus Names:At5g04970
ORF Names:MUG13.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G04970
TAIRilocus:2175334 AT5G04970

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000037169520 – 624Probable pectinesterase/pectinesterase inhibitor 47Add BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi330N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi369N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi376N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi387N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi452 ↔ 472By similarity
Glycosylationi505N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi555N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi596N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi601N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FF77
PRIDEiQ9FF77

Expressioni

Gene expression databases

ExpressionAtlasiQ9FF77 baseline and differential
GenevisibleiQ9FF77 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G04970.1

Structurei

3D structure databases

ProteinModelPortaliQ9FF77
SMRiQ9FF77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 236Pectinesterase inhibitor 47Add BLAST163
Regioni307 – 606Pectinesterase 47Add BLAST300

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 94Pro-richAdd BLAST71

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGST Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9FF77
KOiK01051
OMAiYGEFNNY
OrthoDBiEOG093605R9
PhylomeDBiQ9FF77

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FF77-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQTHSSSLVF LALLCLSWAL LVSPTRPPSQ PPSHPPIQPS SQPPTQPPSQ
60 70 80 90 100
PPTQPPTQPP SHPPTQPPTP PPSQSPSQPS PLPPNIACKS TPYPKLCRTI
110 120 130 140 150
LSAVKSSPSD PYHYGKFTMK QCLKQARRLS KVINRFAQRV EADPGTSTVE
160 170 180 190 200
EVSAVADCGE LAELSVEYLE TVTEELKAAE LMTAALVDRV TSLLGGVVTN
210 220 230 240 250
QQTCLDGLVD AKSGFATAIG TPLGNLTRLY SVSLGLVSHA LNRNLKRYKG
260 270 280 290 300
SKGKIFGGGN KPVREPLETL IKVLRKTCDK GKDCRKANRN LGELGETSGG
310 320 330 340 350
SILVREAVTV GPYETDNFPT ITEAVAAAPN HTFPEQGYFV IYARAGLYEE
360 370 380 390 400
YVVISNKKRN IMLIGDGINK TIISGNHSFI DGWTTYNSST FAVVGDRFVA
410 420 430 440 450
VDVTFRNTAG PEKHQAVAVR NNADGSTFYR CSFEGYQDTL YVHSLRQFYR
460 470 480 490 500
ECDIYGTIDF IFGNAAAIFQ NCNIYARKPM ANQKNAVTAH GRTDPNQKTG
510 520 530 540 550
ISIINCTIGA APDLAADPKS TMTFLGRPWK PYSRTVYIQS YISDVVQPVG
560 570 580 590 600
WLEWNGTTGL DTISYGEYDN FGPGADTSKR VQWSGYSLLN LVQAMNFTVY
610 620
NFTLGDTWLP QTDIPFYGGL LHTE
Length:624
Mass (Da):68,148
Last modified:March 1, 2001 - v1
Checksum:i80D388C903AC5E9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005245 Genomic DNA Translation: BAB11519.1
CP002688 Genomic DNA Translation: AED90810.1
RefSeqiNP_196116.1, NM_120579.3
UniGeneiAt.27773

Genome annotation databases

EnsemblPlantsiAT5G04970.1; AT5G04970.1; AT5G04970
GeneIDi830379
GrameneiAT5G04970.1; AT5G04970.1; AT5G04970
KEGGiath:AT5G04970

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005245 Genomic DNA Translation: BAB11519.1
CP002688 Genomic DNA Translation: AED90810.1
RefSeqiNP_196116.1, NM_120579.3
UniGeneiAt.27773

3D structure databases

ProteinModelPortaliQ9FF77
SMRiQ9FF77
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G04970.1

Proteomic databases

PaxDbiQ9FF77
PRIDEiQ9FF77

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G04970.1; AT5G04970.1; AT5G04970
GeneIDi830379
GrameneiAT5G04970.1; AT5G04970.1; AT5G04970
KEGGiath:AT5G04970

Organism-specific databases

AraportiAT5G04970
TAIRilocus:2175334 AT5G04970

Phylogenomic databases

eggNOGiENOG410IGST Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9FF77
KOiK01051
OMAiYGEFNNY
OrthoDBiEOG093605R9
PhylomeDBiQ9FF77

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

BioCyciARA:AT5G04970-MONOMER

Miscellaneous databases

PROiPR:Q9FF77

Gene expression databases

ExpressionAtlasiQ9FF77 baseline and differential
GenevisibleiQ9FF77 AT

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPME47_ARATH
AccessioniPrimary (citable) accession number: Q9FF77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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