UniProtKB - Q9FF55 (PDI14_ARATH)
Protein
Protein disulfide isomerase-like 1-4
Gene
PDIL1-4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.1 Publication
Catalytic activityi
- Catalyzes the rearrangement of -S-S- bonds in proteins. EC:5.3.4.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 132 | NucleophileBy similarity | 1 | |
Sitei | 133 | Contributes to redox potential valueBy similarity | 1 | |
Sitei | 134 | Contributes to redox potential valueBy similarity | 1 | |
Active sitei | 135 | NucleophileBy similarity | 1 | |
Sitei | 194 | Lowers pKa of C-terminal Cys of first active siteBy similarity | 1 | |
Active sitei | 471 | NucleophileBy similarity | 1 | |
Sitei | 472 | Contributes to redox potential valueBy similarity | 1 | |
Sitei | 473 | Contributes to redox potential valueBy similarity | 1 | |
Active sitei | 474 | NucleophileBy similarity | 1 | |
Sitei | 536 | Lowers pKa of C-terminal Cys of second active siteBy similarity | 1 |
GO - Molecular functioni
- protein disulfide isomerase activity Source: TAIR
GO - Biological processi
- protein folding Source: GO_Central
- response to endoplasmic reticulum stress Source: GO_Central
- response to oxidative stress Source: TAIR
Keywordsi
Molecular function | Isomerase |
Names & Taxonomyi
Protein namesi | Recommended name: Protein disulfide isomerase-like 1-4 (EC:5.3.4.1)Short name: AtPDIL1-4 Alternative name(s): Protein disulfide isomerase 2 Short name: AtPDI2 Protein disulfide isomerase-like 2-2 Short name: AtPDIL2-2 |
Gene namesi | Name:PDIL1-4 Synonyms:PDI2, PDIL2-2 Ordered Locus Names:At5g60640 ORF Names:MUP24.6 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G60640 |
TAIRi | locus:2175811, AT5G60640 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation1 Publication
Nucleus
- Nucleus 1 Publication
Vacuole
- Vacuole 1 Publication
Golgi apparatus
- Golgi apparatus 1 Publication
Cell wall
- cell wall 1 Publication
Cell Wall
- cell wall Source: UniProtKB-SubCell
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Mitochondrion
- mitochondrion Source: TAIR
Nucleus
- nucleus Source: UniProtKB-SubCell
Vacuole
- vacuolar membrane Source: TAIR
Other locations
- plastid Source: TAIR
- secretory vesicle Source: TAIR
Keywords - Cellular componenti
Cell wall, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, VacuolePathology & Biotechi
Disruption phenotypei
No visible phenotype, probably due to functional redundancy.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000400019 | 26 – 597 | Protein disulfide isomerase-like 1-4Add BLAST | 572 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 112 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 132 ↔ 135 | Redox-activePROSITE-ProRule annotation | ||
Glycosylationi | 213 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 342 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 471 ↔ 474 | Redox-activePROSITE-ProRule annotation | ||
Glycosylationi | 524 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9FF55 |
PRIDEi | Q9FF55 |
ProteomicsDBi | 236290 [Q9FF55-1] |
PTM databases
iPTMneti | Q9FF55 |
SwissPalmi | Q9FF55 |
Expressioni
Tissue specificityi
Expressed in germinating seedling, including the cotyledons and hypocotyl, in vascular tissues, in pollen grains, root tips, leaf trichomes, developing seeds and siliques.2 Publications
Gene expression databases
ExpressionAtlasi | Q9FF55, baseline and differential |
Genevisiblei | Q9FF55, AT |
Interactioni
Subunit structurei
Interacts with MEE8 and MED37A.
1 PublicationProtein-protein interaction databases
BioGRIDi | 21429, 11 interactors |
STRINGi | 3702.AT5G60640.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 85 – 208 | Thioredoxin 1PROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 429 – 550 | Thioredoxin 2PROSITE-ProRule annotationAdd BLAST | 122 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 594 – 597 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Sequence similaritiesi
Belongs to the protein disulfide isomerase family.Curated
Keywords - Domaini
Redox-active center, Repeat, SignalPhylogenomic databases
eggNOGi | KOG0190, Eukaryota |
InParanoidi | Q9FF55 |
PhylomeDBi | Q9FF55 |
Family and domain databases
Gene3Di | 3.40.30.10, 4 hits |
InterProi | View protein in InterPro IPR005788, Disulphide_isomerase IPR005792, Prot_disulphide_isomerase IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 2 hits |
SUPFAMi | SSF52833, SSF52833, 4 hits |
TIGRFAMsi | TIGR01130, ER_PDI_fam, 1 hit TIGR01126, pdi_dom, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS00194, THIOREDOXIN_1, 2 hits PS51352, THIOREDOXIN_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9FF55-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAFRVLLLFS LTALLIFSAV SPSFAASSSD DVDDEDLSFL EDLKEDDVPG
60 70 80 90 100
ADSLSSSTGF DEFEGGEEED PDMYNDDDDE EGDFSDLGNP DSDPLPTPEI
110 120 130 140 150
DEKDVVVIKE RNFTDVIENN QYVLVEFYAP WCGHCQSLAP EYAAAATELK
160 170 180 190 200
EDGVVLAKID ATEENELAQE YRVQGFPTLL FFVDGEHKPY TGGRTKETIV
210 220 230 240 250
TWVKKKIGPG VYNLTTLDDA EKVLTSGNKV VLGYLNSLVG VEHDQLNAAS
260 270 280 290 300
KAEDDVNFYQ TVNPDVAKMF HLDPESKRPA LVLVKKEEEK ISHFDGEFVK
310 320 330 340 350
SALVSFVSAN KLALVSVFTR ETAPEIFESA IKKQLLLFVT KNESEKVLTE
360 370 380 390 400
FQEAAKSFKG KLIFVSVDLD NEDYGKPVAE YFGVSGNGPK LIGYTGNEDP
410 420 430 440 450
KKYFFDGEIQ SDKIKIFGED FLNDKLKPFY KSDPIPEKND EDVKIVVGDN
460 470 480 490 500
FDEIVLDDSK DVLLEVYAPW CGHCQALEPM YNKLAKHLRS IDSLVITKMD
510 520 530 540 550
GTTNEHPKAK AEGFPTILFF PAGNKTSEPI TVDTDRTVVA FYKFLRKHAT
560 570 580 590
IPFKLEKPAS TESPKTAEST PKVETTETKE SPDSTTKSSQ SDSKDEL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4K0F7 | F4K0F7_ARATH | Protein disulfide-isomerase | PDIL1-4 ATPDI2, ATPDIL1-4, MUP24.6, MUP24_6, PDI-like 1-4 | 536 | Annotation score: | ||
F4K0F5 | F4K0F5_ARATH | Protein disulfide-isomerase | PDIL1-4 ATPDI2, ATPDIL1-4, MUP24.6, MUP24_6, PDI-like 1-4 | 533 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 400 | P → H in AAM65262 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB005246 Genomic DNA Translation: BAB09837.1 CP002688 Genomic DNA Translation: AED97360.1 BT001994 mRNA Translation: AAN72005.1 BT008359 mRNA Translation: AAP37718.1 AY087725 mRNA Translation: AAM65262.1 |
RefSeqi | NP_851234.1, NM_180903.4 [Q9FF55-1] |
Genome annotation databases
EnsemblPlantsi | AT5G60640.1; AT5G60640.1; AT5G60640 [Q9FF55-1] |
GeneIDi | 836185 |
Gramenei | AT5G60640.1; AT5G60640.1; AT5G60640 [Q9FF55-1] |
KEGGi | ath:AT5G60640 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB005246 Genomic DNA Translation: BAB09837.1 CP002688 Genomic DNA Translation: AED97360.1 BT001994 mRNA Translation: AAN72005.1 BT008359 mRNA Translation: AAP37718.1 AY087725 mRNA Translation: AAM65262.1 |
RefSeqi | NP_851234.1, NM_180903.4 [Q9FF55-1] |
3D structure databases
SMRi | Q9FF55 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 21429, 11 interactors |
STRINGi | 3702.AT5G60640.1 |
PTM databases
iPTMneti | Q9FF55 |
SwissPalmi | Q9FF55 |
Proteomic databases
PaxDbi | Q9FF55 |
PRIDEi | Q9FF55 |
ProteomicsDBi | 236290 [Q9FF55-1] |
Genome annotation databases
EnsemblPlantsi | AT5G60640.1; AT5G60640.1; AT5G60640 [Q9FF55-1] |
GeneIDi | 836185 |
Gramenei | AT5G60640.1; AT5G60640.1; AT5G60640 [Q9FF55-1] |
KEGGi | ath:AT5G60640 |
Organism-specific databases
Araporti | AT5G60640 |
TAIRi | locus:2175811, AT5G60640 |
Phylogenomic databases
eggNOGi | KOG0190, Eukaryota |
InParanoidi | Q9FF55 |
PhylomeDBi | Q9FF55 |
Miscellaneous databases
PROi | PR:Q9FF55 |
Gene expression databases
ExpressionAtlasi | Q9FF55, baseline and differential |
Genevisiblei | Q9FF55, AT |
Family and domain databases
Gene3Di | 3.40.30.10, 4 hits |
InterProi | View protein in InterPro IPR005788, Disulphide_isomerase IPR005792, Prot_disulphide_isomerase IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 2 hits |
SUPFAMi | SSF52833, SSF52833, 4 hits |
TIGRFAMsi | TIGR01130, ER_PDI_fam, 1 hit TIGR01126, pdi_dom, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS00194, THIOREDOXIN_1, 2 hits PS51352, THIOREDOXIN_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDI14_ARATH | |
Accessioni | Q9FF55Primary (citable) accession number: Q9FF55 Secondary accession number(s): Q8LAM5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2010 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families