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Protein

Serine/threonine-protein kinase PBS1

Gene

PBS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein kinase required for plant defense mechanism mediated by the disease resistance (R) protein RPS5. In case of infection by Pseudomonas syringae, AvrPphB triggers RPS5-mediated defense mechanism via the cleavage of PBS1. Both kinase activity and cleavage by avrPphB are independently required to trigger the RPS5-mediated resistance. Contributes to PAMP-triggered immunity (PTI) signaling and defense responses downstream of FLS2.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115ATPPROSITE-ProRule annotation1
Active sitei213Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 100ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR
  • transmembrane receptor protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processPlant defense
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PBS11 Publication (EC:2.7.11.11 Publication)
Alternative name(s):
AvrPphB susceptible protein 11 Publication
Gene namesi
Name:PBS11 Publication
Ordered Locus Names:At5g13160Imported
ORF Names:T19L5.120Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G13160
TAIRilocus:2179857 AT5G13160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduction in H2O2 accumulation and callose deposits.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Slightly affects plasma membrane location. Abolishes plasma membrane location; when associated with A-3 and A-6. 2 Publications1
Mutagenesisi3C → A: Affects plasma membrane location. Abolishes plasma membrane location; when associated with A-2 and A-6. 2 Publications1
Mutagenesisi4F → A: Affects plasma membrane location. 1 Publication1
Mutagenesisi6C → A: Affects plasma membrane location. Abolishes plasma membrane location; when associated with A-2 and A-3. 2 Publications1
Mutagenesisi98G → E in pbs1-5; strongly impairs RPS5-mediated plant resistance to P.syringae. 1 Publication1
Mutagenesisi103G → R in pbs1-3; strongly impairs cleavage by avrPphB and RPS5-mediated plant resistance to P.syringae. 1 Publication1
Mutagenesisi115K → N: Abolishes kinase activity and RPS5-mediated plant resistance to P.syringae. 3 Publications1
Mutagenesisi241G → A: Abolishes cleavage by avrPphB and RPS5-mediated plant resistance to P.syringae. 1 Publication1
Mutagenesisi242D → A: Strongly impairs cleavage by avrPphB and RPS5-mediated plant resistance to P.syringae. 1 Publication1
Mutagenesisi243K → A: Affects cleavage by avrPphB and RPS5-mediated plant resistance to P.syringae. 1 Publication1
Mutagenesisi244S → F in pbs1-6; strongly impairs RPS5-mediated plant resistance to P.syringae. 1 Publication1
Mutagenesisi252G → R in pbs1-2; strongly impairs RPS5-mediated plant resistance to P.syringae. 3 Publications1
Mutagenesisi286G → D in pbs1-4; strongly impairs cleavage by avrPphB and RPS5-mediated plant resistance to P.syringae. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000864822 – 456Serine/threonine-protein kinase PBS1Add BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi3S-palmitoyl cysteine1 Publication1
Lipidationi6S-palmitoyl cysteine1 Publication1
Modified residuei21PhosphoserineCombined sources1
Modified residuei160PhosphotyrosineBy similarity1
Modified residuei217PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1
Modified residuei248PhosphothreonineBy similarity1
Modified residuei253PhosphothreonineBy similarity1
Modified residuei261PhosphotyrosineBy similarity1

Post-translational modificationi

Cleaved by avrPphB in infected plant cells. Its cleavage serves as a signal that triggers the RPS5-mediated defense system.2 Publications
Autophosphorylates (Probable). Autophosphorylation may be required to trigger the RPS5-mediated plant defense system.Curated
Palmitoylation at Cys-3 and Cys-6 are required for plasma membrane location that is essential for the RPS5-mediated plant defense response.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei243 – 244Cleavage; by avrPphB2

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9FE20
PRIDEiQ9FE20

PTM databases

iPTMnetiQ9FE20
SwissPalmiQ9FE20

Miscellaneous databases

PMAP-CutDBiQ9FE20

Expressioni

Inductioni

Induced by flagellin (flg22).1 Publication

Gene expression databases

ExpressionAtlasiQ9FE20 baseline and differential
GenevisibleiQ9FE20 AT

Interactioni

Subunit structurei

In infected plant cells, it interacts with the P.syringae virulence protein avrPphB. In uninfected plants, autophosphorylated form interacts with RPS5. Interacts with FLS2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPS5O649732EBI-2357898,EBI-15620767

Protein-protein interaction databases

BioGridi16433, 2 interactors
DIPiDIP-53310N
IntActiQ9FE20, 3 interactors
STRINGi3702.AT5G13160.1

Structurei

3D structure databases

ProteinModelPortaliQ9FE20
SMRiQ9FE20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 363Protein kinasePROSITE-ProRule annotationAdd BLAST278

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi292 – 296Recognition motif required for RPS5-mediated plant resistance to P.syringae1 Publication5

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1187 Eukaryota
COG0515 LUCA
HOGENOMiHOG000116550
InParanoidiQ9FE20
KOiK13430
OMAiRERKQPN
OrthoDBiEOG093609F5
PhylomeDBiQ9FE20

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FE20-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGCFSCFDSS DDEKLNPVDE SNHGQKKQSQ PTVSNNISGL PSGGEKLSSK
60 70 80 90 100
TNGGSKRELL LPRDGLGQIA AHTFAFRELA AATMNFHPDT FLGEGGFGRV
110 120 130 140 150
YKGRLDSTGQ VVAVKQLDRN GLQGNREFLV EVLMLSLLHH PNLVNLIGYC
160 170 180 190 200
ADGDQRLLVY EFMPLGSLED HLHDLPPDKE ALDWNMRMKI AAGAAKGLEF
210 220 230 240 250
LHDKANPPVI YRDFKSSNIL LDEGFHPKLS DFGLAKLGPT GDKSHVSTRV
260 270 280 290 300
MGTYGYCAPE YAMTGQLTVK SDVYSFGVVF LELITGRKAI DSEMPHGEQN
310 320 330 340 350
LVAWARPLFN DRRKFIKLAD PRLKGRFPTR ALYQALAVAS MCIQEQAATR
360 370 380 390 400
PLIADVVTAL SYLANQAYDP SKDDSRRNRD ERGARLITRN DDGGGSGSKF
410 420 430 440 450
DLEGSEKEDS PRETARILNR DINRERAVAE AKMWGESLRE KRRQSEQGTS

ESNSTG
Length:456
Mass (Da):50,384
Last modified:March 1, 2001 - v1
Checksum:iC167E4E3F47ACBC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314176 mRNA Translation: AAG38109.1
AL391711 Genomic DNA Translation: CAC05444.1
CP002688 Genomic DNA Translation: AED91858.1
AF372927 mRNA Translation: AAK50067.1
AY078037 mRNA Translation: AAL77738.1
RefSeqiNP_196820.1, NM_121319.5
UniGeneiAt.23518

Genome annotation databases

EnsemblPlantsiAT5G13160.1; AT5G13160.1; AT5G13160
GeneIDi831155
GrameneiAT5G13160.1; AT5G13160.1; AT5G13160
KEGGiath:AT5G13160

Similar proteinsi

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314176 mRNA Translation: AAG38109.1
AL391711 Genomic DNA Translation: CAC05444.1
CP002688 Genomic DNA Translation: AED91858.1
AF372927 mRNA Translation: AAK50067.1
AY078037 mRNA Translation: AAL77738.1
RefSeqiNP_196820.1, NM_121319.5
UniGeneiAt.23518

3D structure databases

ProteinModelPortaliQ9FE20
SMRiQ9FE20
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16433, 2 interactors
DIPiDIP-53310N
IntActiQ9FE20, 3 interactors
STRINGi3702.AT5G13160.1

PTM databases

iPTMnetiQ9FE20
SwissPalmiQ9FE20

Proteomic databases

PaxDbiQ9FE20
PRIDEiQ9FE20

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G13160.1; AT5G13160.1; AT5G13160
GeneIDi831155
GrameneiAT5G13160.1; AT5G13160.1; AT5G13160
KEGGiath:AT5G13160

Organism-specific databases

AraportiAT5G13160
TAIRilocus:2179857 AT5G13160

Phylogenomic databases

eggNOGiKOG1187 Eukaryota
COG0515 LUCA
HOGENOMiHOG000116550
InParanoidiQ9FE20
KOiK13430
OMAiRERKQPN
OrthoDBiEOG093609F5
PhylomeDBiQ9FE20

Miscellaneous databases

PMAP-CutDBiQ9FE20
PROiPR:Q9FE20

Gene expression databases

ExpressionAtlasiQ9FE20 baseline and differential
GenevisibleiQ9FE20 AT

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPBS1_ARATH
AccessioniPrimary (citable) accession number: Q9FE20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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