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UniProtKB - Q9FAW5 (AZRB_BACOY)
Protein
NADPH azoreductase
Gene
azr
Organism
Bacillus sp. (strain OY1-2)
Status
Functioni
Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADPH as an electron donor for its activity. Compounds with paired naphthalene groups coupled with the azo group are good substrates, with the following preference order: Rocceline > Sumifix Black B > Solar Orange.
Catalytic activityi
- aniline + N,N-dimethyl-1,4-phenylenediamine + 2 NADP+ = 4-(dimethylamino)azobenzene + 2 H+ + 2 NADPHEC:1.7.1.6
Temperature dependencei
Optimum temperature is 70 degrees Celsius.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 106 – 111 | NADPSequence analysis | 6 |
GO - Molecular functioni
- azobenzene reductase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.7.1.6, 691 |
SABIO-RKi | Q9FAW5 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:azr |
Organismi | Bacillus sp. (strain OY1-2) |
Taxonomic identifieri | 104667 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000234085 | 1 – 178 | NADPH azoreductaseAdd BLAST | 178 |
Expressioni
Inductioni
Constitutively expressed.
Interactioni
Subunit structurei
Monomer.
Family & Domainsi
Sequence similaritiesi
Belongs to the azoreductase type 2 family.Curated
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR029039, Flavoprotein-like_sf IPR005025, FMN_Rdtase-like |
Pfami | View protein in Pfam PF03358, FMN_red, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9FAW5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLVVINGTP RKFGRTRVVA KYIADQFEGE LYDLAIEELP LYNGEESQRD
60 70 80 90 100
LEAVKKLKTL VKAADGVVLC TPEYHNAMSG ALKNSLDYLS SSEFIHKPVA
110 120 130 140 150
LLAVAGGGKG GINALNSMHA SLAGVYANAI PKQVVLDGLH VQDGELGEDA
160 170
KPLIHDVVKE LKAYMSVYKE VKKQLGVE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032601 Genomic DNA Translation: BAB13746.1 |
Genome annotation databases
KEGGi | ag:BAB13746 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032601 Genomic DNA Translation: BAB13746.1 |
3D structure databases
SMRi | Q9FAW5 |
ModBasei | Search... |
Chemistry databases
DrugBanki | DB01014, Balsalazide |
Genome annotation databases
KEGGi | ag:BAB13746 |
Enzyme and pathway databases
BRENDAi | 1.7.1.6, 691 |
SABIO-RKi | Q9FAW5 |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR029039, Flavoprotein-like_sf IPR005025, FMN_Rdtase-like |
Pfami | View protein in Pfam PF03358, FMN_red, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AZRB_BACOY | |
Accessioni | Q9FAW5Primary (citable) accession number: Q9FAW5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 46 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families