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Entry version 62 (11 Dec 2019)
Sequence version 1 (01 Mar 2001)
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Protein

N,N'-diacetylchitobiose phosphorylase

Gene

chbP

Organism
Vibrio furnissii
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible phosphorolysis of chitobiose (N,N'-diacetylchitobiose or (GlcNAc)2) into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.58 mM for N,N'-diacetylchitobiose1 Publication
  2. KM=0.51 mM for phosphate1 Publication
  1. Vmax=4.6 nmol/min/µg enzyme with N,N'-diacetylchitobiose as substrate1 Publication
  2. Vmax=4.8 nmol/min/µg enzyme with phosphate as substrate1 Publication

pH dependencei

Optimum pH is 6.5-7.0.1 Publication

Temperature dependencei

Optimum temperature is 20-37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi140Calcium 3By similarity1
Metal bindingi186Calcium 1By similarity1
Metal bindingi187Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi190Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi196Calcium 1; via carbonyl oxygenBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei333SubstrateBy similarity1
Binding sitei343SubstrateBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei489NucleophileBy similarity1
Binding sitei637SubstrateBy similarity1
Binding sitei644SubstrateBy similarity1
Binding sitei690SubstrateBy similarity1
Metal bindingi790Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi792Calcium 2By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER-16878

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.4.1.280 6631

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
GH94 Glycoside Hydrolase Family 94

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
N,N'-diacetylchitobiose phosphorylase (EC:2.4.1.280)
Alternative name(s):
Chitobiose phosphorylase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:chbP
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiVibrio furnissii
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri29494 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004241081 – 800N,N'-diacetylchitobiose phosphorylaseAdd BLAST800

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q9F8X1

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

By similarity

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9F8X1

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni349 – 350Substrate bindingBy similarity2
Regioni490 – 492Substrate bindingBy similarity3
Regioni709 – 710Substrate bindingBy similarity2

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycosyl hydrolase 94 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105E2V Bacteria
COG3459 LUCA

KEGG Orthology (KO)

More...
KOi
K18675

Family and domain databases

Conserved Domains Database

More...
CDDi
cd11755 GH94N_ChBP_like, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.50.10.10, 1 hit
2.70.98.40, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR009342 Carb-bd_put_dom
IPR011013 Gal_mutarotase_sf_dom
IPR033432 GH36_catalytic
IPR037828 GH94N_ChBP
IPR037018 Glyco_hydro_65_N_sf
IPR010383 Glyco_hydrolase_94

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF17167 Glyco_hydro_36, 1 hit
PF06165 Glyco_transf_36, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01068 CBM_X, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48208 SSF48208, 1 hit
SSF74650 SSF74650, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9F8X1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKYGYFDNDN REYVITRPDV PAPWTNYLGT EKFCTVISHN AGGYSFYHSP
60 70 80 90 100
EYNRVTKFRP NFTQDRPGHY IYLRDDETGD FWSVSWQPVA KNLDDAHYEV
110 120 130 140 150
RHGLSYSKFR CDYNGIVATK TLFVPKGEDA QVWDVEIENT SDQPRTISAF
160 170 180 190 200
GYVEFSFSHI ASDNQNHQMS LYSAGTEYNN GVLEYDLYYN TDDFLGFYYL
210 220 230 240 250
TATFDADSYD GQRDAFLGMY RDEANPIAVA NGRCSNSAQT CYNHCGALHK
260 270 280 290 300
QFVLQPGEKV RFAVILGVGK GNGEKLRAKY QDLSQVDAAF AGIKQHWDER
310 320 330 340 350
CAKFQVRSPN QGLDTMINAW TLYQAETCVV WSRFASFIEV GGRTGLGYRD
360 370 380 390 400
TAQDAISVPH TNPAMTRKRL VDLLRGQVKA GYGLHLFDPD WFDPEKADVK
410 420 430 440 450
PSKSPTVVPT PSDEDKIHGI KDTCSDDHLW IVPTILNFVK ETGDLSFIDE
460 470 480 490 500
VIPYADGGDA TVYQHMMAAL DFSAEYVGQT GICKGLRADW NDCLNLGGGE
510 520 530 540 550
SAMVSFLHFW ALEAFLELAR HRQDAAAIDK YQAMANGVRE ACETHLWDDN
560 570 580 590 600
GGWYIRGLTK DGDKIGTFEQ QEGKVHLESN TLAVLSGAVS QQRGEKAMDA
610 620 630 640 650
VYEYLFSPYG LHLNAPSFAT PNDDIGFVTR VYQGVKENGA IFSHPNPWAW
660 670 680 690 700
VAEAKLGRGD RAMEFYDSLN PYNQNDIIET RVAEPYSYVQ FIMGRDHQDH
710 720 730 740 750
GRANHPWLTG TSGWAYYATT NFILGVRTGF DTLTVDPCIP AAWSGFEVTR
760 770 780 790 800
EWRGATYHIS VQNPNGVSKG VQSILVNGEA VDAINAQPAG SENQVTVILG
Length:800
Mass (Da):89,667
Last modified:March 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6775D823C051122A
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF230379 Genomic DNA Translation: AAG23740.1

Genome annotation databases

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ag:AAG23740

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230379 Genomic DNA Translation: AAG23740.1

3D structure databases

SMRiQ9F8X1
ModBaseiSearch...

Protein family/group databases

CAZyiGH94 Glycoside Hydrolase Family 94

Proteomic databases

PRIDEiQ9F8X1

Genome annotation databases

KEGGiag:AAG23740

Phylogenomic databases

eggNOGiENOG4105E2V Bacteria
COG3459 LUCA
KOiK18675

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16878
BRENDAi2.4.1.280 6631

Family and domain databases

CDDicd11755 GH94N_ChBP_like, 1 hit
Gene3Di1.50.10.10, 1 hit
2.70.98.40, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR009342 Carb-bd_put_dom
IPR011013 Gal_mutarotase_sf_dom
IPR033432 GH36_catalytic
IPR037828 GH94N_ChBP
IPR037018 Glyco_hydro_65_N_sf
IPR010383 Glyco_hydrolase_94
PfamiView protein in Pfam
PF17167 Glyco_hydro_36, 1 hit
PF06165 Glyco_transf_36, 1 hit
SMARTiView protein in SMART
SM01068 CBM_X, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit
SSF74650 SSF74650, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCHBP_VIBFU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9F8X1
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: March 1, 2001
Last modified: December 11, 2019
This is version 62 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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