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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei262ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-315-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CT0299
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195411 – 503Glutamate--tRNA ligaseAdd BLAST503

Proteomic databases

PRIDEiQ9F724

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0299

Structurei

3D structure databases

ProteinModelPortaliQ9F724
SMRiQ9F724
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi259 – 263"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9F724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGQKVRTRF APSPTGYLHV GGLRTALYNY LFAKRMNGDF VIRIEDTDQS
60 70 80 90 100
RKVEDAEKKL ISTLEWAGII ADESPMHGGN YGPYVQSQRL SIYRDYCTRL
110 120 130 140 150
LEDKNAYYCF STPEELEENR QLQLKQGLQP KYNRKWLPED MGGNMPESEI
160 170 180 190 200
KKKLDEGAPY VVRMKVPDYV SVWFEDMIRG PIEFDSATID DQVLMKSDGF
210 220 230 240 250
PTYHFASVID DHLMEFTHII RGEEWLPSMP KHLLLYEFFG WEPPKFAHLP
260 270 280 290 300
LLLNPDRSKL SKRQGDVAVE DYMRKGYSSE AIVNFVALLG WNEGEGSEQE
310 320 330 340 350
VFSMEELISK FSLERVGKAG AVFNVEKLSW LEKQYIKTRP VEKIVGNIKP
360 370 380 390 400
VLQAKLAEFS PEMSVERITS DDYLAKVVEL MRERVNFEHE FVTFSSYFFF
410 420 430 440 450
EPESYEEEAV AKRWTPNVPP LLQEFADLLE ANDDFTAENI EAQLKAFVAP
460 470 480 490 500
KGLKPAVLIH PIRIAVSGVS FGPSLYHMLE VLGKEAVLRR IRRAIERIEV

PAA
Length:503
Mass (Da):57,810
Last modified:August 30, 2002 - v2
Checksum:i8A265B64E72F4D80
GO

Sequence cautioni

The sequence AAG12192 differs from that shown. Reason: Frameshift at position 317.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM71545.1
AF287480 Genomic DNA Translation: AAG12192.1 Frameshift.
RefSeqiNP_661203.1, NC_002932.3
WP_010931991.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM71545; AAM71545; CT0299
GeneIDi1007958
KEGGicte:CT0299
PATRICifig|194439.7.peg.290

Similar proteinsi

Entry informationi

Entry nameiSYE_CHLTE
AccessioniPrimary (citable) accession number: Q9F724
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 30, 2002
Last modified: March 28, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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