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Protein

23S rRNA (uridine(2479)-2'-O)-methyltransferase

Gene

aviRb

Organism
Streptomyces viridochromogenes
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + uridine(2479) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2479) in 23S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232S-adenosyl-L-methionine; via amide nitrogen1 Publication1

GO - Molecular functioni

  • RNA binding Source: InterPro
  • rRNA (uridine-2'-O-)-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • methylation Source: UniProtKB
  • response to antibiotic Source: UniProtKB
  • rRNA methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAntibiotic resistance
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16328
BRENDAi2.1.1.208 6116

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (uridine(2479)-2'-O)-methyltransferase (EC:2.1.1.208)
Alternative name(s):
Avilamycin resistance protein B
Gene namesi
Name:aviRb
OrganismiStreptomyces viridochromogenes
Taxonomic identifieri1938 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi135T → V: Slightly increases catalytic activity. 1 Publication1
Mutagenesisi139N → A: Almost abolishes catalytic activity. 1 Publication1
Mutagenesisi145R → A: Almost abolishes catalytic activity. 1 Publication1
Mutagenesisi234E → A: Strongly impairs catalytic activity. 1 Publication1
Mutagenesisi234E → Q: Slightly impairs catalytic activity. 1 Publication1
Mutagenesisi262N → A: Almost abolishes catalytic activity. 1 Publication1
Mutagenesisi262N → Q: Slightly impairs catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004184611 – 28723S rRNA (uridine(2479)-2'-O)-methyltransferaseAdd BLAST287

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 27Combined sources8
Helixi32 – 38Combined sources7
Beta strandi40 – 45Combined sources6
Helixi46 – 54Combined sources9
Beta strandi59 – 67Combined sources9
Helixi72 – 80Combined sources9
Beta strandi83 – 88Combined sources6
Helixi90 – 93Combined sources4
Beta strandi95 – 97Combined sources3
Beta strandi99 – 101Combined sources3
Beta strandi105 – 111Combined sources7
Helixi117 – 119Combined sources3
Beta strandi127 – 133Combined sources7
Helixi137 – 149Combined sources13
Beta strandi154 – 162Combined sources9
Helixi167 – 172Combined sources6
Turni173 – 175Combined sources3
Helixi176 – 178Combined sources3
Beta strandi182 – 187Combined sources6
Helixi188 – 201Combined sources14
Beta strandi206 – 210Combined sources5
Beta strandi215 – 217Combined sources3
Helixi218 – 220Combined sources3
Beta strandi227 – 231Combined sources5
Turni234 – 236Combined sources3
Helixi240 – 245Combined sources6
Beta strandi247 – 251Combined sources5
Beta strandi255 – 258Combined sources4
Helixi263 – 278Combined sources16

3D structure databases

ProteinModelPortaliQ9F5K6
SMRiQ9F5K6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9F5K6

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 211S-adenosyl-L-methionine binding2
Regioni252 – 254S-adenosyl-L-methionine binding3

Sequence similaritiesi

Phylogenomic databases

KOiK21514

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.40.1280.10, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR029064 L30e-like
IPR001537 SpoU_MeTrfase
IPR029026 tRNA_m1G_MTases_N
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
SSF75217 SSF75217, 1 hit

Sequencei

Sequence statusi: Complete.

Q9F5K6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSRGERTP AARRITSRNA RFQQWQALLG NRNKRTRAGE FLVMGVRPIS
60 70 80 90 100
LAVEHGWPVR TLLYDGQREL SKWARELLRT VRTEQIAMAP DLLMELGEKN
110 120 130 140 150
EAPPEVVAVV EMPADDLDRI PVREDFLGVL FDRPTSPGNI GSIIRSADAL
160 170 180 190 200
GAHGLIVAGH AADVYDPKSV RSSTGSLFSL PAVRVPSPGE VMDWVEARRA
210 220 230 240 250
AGTPIVLVGT DEHGDCDVFD FDFTQPTLLL IGNETAGLSN AWRTLCDYTV
260 270 280
SIPMAGSASS LNAANAATAI LYEAVRQRIS GRTATTP
Length:287
Mass (Da):31,215
Last modified:March 1, 2001 - v1
Checksum:i27E742DB22E19DBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF333038 Genomic DNA Translation: AAG32066.1

Genome annotation databases

KEGGiag:AAG32066

Similar proteinsi

Entry informationi

Entry nameiAVRB_STRVR
AccessioniPrimary (citable) accession number: Q9F5K6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 1, 2001
Last modified: September 27, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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