UniProtKB - Q9F5K6 (AVRB_STRVR)
Protein
23S rRNA (uridine(2479)-2'-O)-methyltransferase
Gene
aviRb
Organism
Streptomyces viridochromogenes
Status
Functioni
Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.2 Publications
Catalytic activityi
- S-adenosyl-L-methionine + uridine2479 in 23S rRNA = 2'-O-methyluridine2479 in 23S rRNA + H+ + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.2081 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 232 | S-adenosyl-L-methionine; via amide nitrogen1 Publication | 1 |
GO - Molecular functioni
- RNA binding Source: InterPro
- rRNA (uridine-2'-O-)-methyltransferase activity Source: UniProtKB
GO - Biological processi
- methylation Source: UniProtKB
- response to antibiotic Source: UniProtKB
- rRNA methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Antibiotic resistance |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16328 |
BRENDAi | 2.1.1.208, 6116 |
Names & Taxonomyi
Protein namesi | Recommended name: 23S rRNA (uridine(2479)-2'-O)-methyltransferase (EC:2.1.1.208)Alternative name(s): Avilamycin resistance protein B |
Gene namesi | Name:aviRb |
Organismi | Streptomyces viridochromogenes |
Taxonomic identifieri | 1938 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 135 | T → V: Slightly increases catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 139 | N → A: Almost abolishes catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 145 | R → A: Almost abolishes catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 234 | E → A: Strongly impairs catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 234 | E → Q: Slightly impairs catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 262 | N → A: Almost abolishes catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 262 | N → Q: Slightly impairs catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418461 | 1 – 287 | 23S rRNA (uridine(2479)-2'-O)-methyltransferaseAdd BLAST | 287 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9F5K6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9F5K6 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 210 – 211 | S-adenosyl-L-methionine binding | 2 | |
Regioni | 252 – 254 | S-adenosyl-L-methionine binding | 3 |
Sequence similaritiesi
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TsnR/AvirB family.Curated
Family and domain databases
Gene3Di | 3.30.1330.30, 1 hit 3.40.1280.10, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR029064, L30e-like IPR001537, SpoU_MeTrfase IPR029026, tRNA_m1G_MTases_N |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit |
SUPFAMi | SSF55315, SSF55315, 1 hit SSF75217, SSF75217, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9F5K6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARSRGERTP AARRITSRNA RFQQWQALLG NRNKRTRAGE FLVMGVRPIS
60 70 80 90 100
LAVEHGWPVR TLLYDGQREL SKWARELLRT VRTEQIAMAP DLLMELGEKN
110 120 130 140 150
EAPPEVVAVV EMPADDLDRI PVREDFLGVL FDRPTSPGNI GSIIRSADAL
160 170 180 190 200
GAHGLIVAGH AADVYDPKSV RSSTGSLFSL PAVRVPSPGE VMDWVEARRA
210 220 230 240 250
AGTPIVLVGT DEHGDCDVFD FDFTQPTLLL IGNETAGLSN AWRTLCDYTV
260 270 280
SIPMAGSASS LNAANAATAI LYEAVRQRIS GRTATTP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF333038 Genomic DNA Translation: AAG32066.1 |
Genome annotation databases
KEGGi | ag:AAG32066 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF333038 Genomic DNA Translation: AAG32066.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X7O | X-ray | 2.37 | A/B | 1-287 | [»] | |
1X7P | X-ray | 2.55 | A/B | 1-287 | [»] | |
SMRi | Q9F5K6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:AAG32066 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16328 |
BRENDAi | 2.1.1.208, 6116 |
Miscellaneous databases
EvolutionaryTracei | Q9F5K6 |
Family and domain databases
Gene3Di | 3.30.1330.30, 1 hit 3.40.1280.10, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR029064, L30e-like IPR001537, SpoU_MeTrfase IPR029026, tRNA_m1G_MTases_N |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit |
SUPFAMi | SSF55315, SSF55315, 1 hit SSF75217, SSF75217, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AVRB_STRVR | |
Accessioni | Q9F5K6Primary (citable) accession number: Q9F5K6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 64 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families