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UniProtKB - Q9EZJ8 (SIGA_THEAQ)
Protein
RNA polymerase sigma factor SigA
Gene
sigA
Organism
Thermus aquaticus
Status
Functioni
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
UniRule annotation2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 398 – 417 | H-T-H motifUniRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-UniRule
- sigma factor activity Source: CACAO
GO - Biological processi
- DNA-templated transcription, initiation Source: InterPro
Keywordsi
Molecular function | DNA-binding, Sigma factor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase sigma factor SigAUniRule annotation |
Gene namesi | Name:sigAUniRule annotation |
Organismi | Thermus aquaticus |
Taxonomic identifieri | 271 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423011 | 1 – 438 | RNA polymerase sigma factor SigAAdd BLAST | 438 |
Interactioni
Subunit structurei
Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.
UniRule annotation5 PublicationsProtein-protein interaction databases
DIPi | DIP-49014N |
IntActi | Q9EZJ8, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9EZJ8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9EZJ8 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 69 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 93 – 128 | Sigma-70 factor domain-1Add BLAST | 36 | |
Regioni | 202 – 272 | Sigma-70 factor domain-2Add BLAST | 71 | |
Regioni | 281 – 359 | Sigma-70 factor domain-3Add BLAST | 79 | |
Regioni | 372 – 424 | Sigma-70 factor domain-4Add BLAST | 53 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 226 – 229 | Interaction with polymerase core subunit RpoC | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 30 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 31 – 63 | Acidic residuesSequence analysisAdd BLAST | 33 |
Domaini
Contains 4 domains, connected by flexible linkers. In the active conformation, the domains are in an extended conformation, each making extensive interactions with the RNA polymerase catalytic core (PubMed:11931761 and PubMed:12016306).
In the autoinhibited state, sigma-70 factor domain-1 packs closely together with sigma-70 factor domains-2 and -4, contrary to the extended conformation that is seen when the protein is part of the RNA polymerase holoenzyme.By similarity
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (PubMed:11931761 and PubMed:22136875).
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (PubMed:11931761).1 Publication
Sequence similaritiesi
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
HAMAPi | MF_00963, Sigma70_RpoD_SigA, 1 hit |
InterProi | View protein in InterPro IPR014284, RNA_pol_sigma-70_dom IPR000943, RNA_pol_sigma70 IPR009042, RNA_pol_sigma70_r1_2 IPR007627, RNA_pol_sigma70_r2 IPR007624, RNA_pol_sigma70_r3 IPR007630, RNA_pol_sigma70_r4 IPR013325, RNA_pol_sigma_r2 IPR013324, RNA_pol_sigma_r3/r4-like IPR012760, RNA_pol_sigma_RpoD_C IPR028630, Sigma70_RpoD IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00140, Sigma70_r1_2, 1 hit PF04542, Sigma70_r2, 1 hit PF04539, Sigma70_r3, 1 hit PF04545, Sigma70_r4, 1 hit |
PRINTSi | PR00046, SIGMA70FCT |
SUPFAMi | SSF88659, SSF88659, 2 hits SSF88946, SSF88946, 1 hit |
TIGRFAMsi | TIGR02393, RpoD_Cterm, 1 hit TIGR02937, sigma70-ECF, 1 hit |
PROSITEi | View protein in PROSITE PS00716, SIGMA70_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9EZJ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKSKSKKKA AKAQEVEVKE PVKEPEPLPE LEAAEDLQDL PEPDPELLAS
60 70 80 90 100
EPELEDLADP LDLEGPLEAD LLPEEGLLEE EEEELSLPKV STSDPVRQYL
110 120 130 140 150
HEIGQVPLLT LEEEIDLARK VEEGMEAIKK LSEATGLDQE LIREVVRAKI
160 170 180 190 200
LGTARIQKIP GLKEKPDPKT VEEVDGKLKS LPKELKRYLH IAREGEAARQ
210 220 230 240 250
HLIEANLRLV VSIAKKYTGR GLSFLDLIQE GNQGLIRAVE KFEYKRRFKF
260 270 280 290 300
STYATWWIRQ AINRAIADQA RTIRIPVHMV ETINKLSRTA RQLQQELGRE
310 320 330 340 350
PSYEEIAEAM GPGWDAKRVE ETLKIAQEPV SLETPIGDEK DSFYGDFIPD
360 370 380 390 400
ENLPSPVEAA AQSLLSEELE KALSKLSERE AMVLKLRKGL IDGREHTLEE
410 420 430
VGAYFGVTRE RIRQIENKAL RKLKYHESRT RKLRDFLE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF291720 Genomic DNA Translation: AAG36964.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF291720 Genomic DNA Translation: AAG36964.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KU2 | X-ray | 2.90 | A/B | 92-332 | [»] | |
1KU3 | X-ray | 1.80 | A | 366-438 | [»] | |
1KU7 | X-ray | 2.40 | A/D | 366-438 | [»] | |
1L9U | X-ray | 4.00 | H/Q | 92-438 | [»] | |
1L9Z | X-ray | 6.50 | H | 1-438 | [»] | |
1RIO | X-ray | 2.30 | H | 366-438 | [»] | |
3LES | X-ray | 2.77 | A/B | 93-271 | [»] | |
3LEV | X-ray | 2.50 | A | 93-271 | [»] | |
3N97 | X-ray | 3.25 | A/D | 366-438 | [»] | |
3UGO | X-ray | 2.10 | A | 92-332 | [»] | |
3UGP | X-ray | 2.70 | A | 92-332 | [»] | |
4KI2 | X-ray | 3.61 | A/B | 92-332 | [»] | |
4XLN | X-ray | 4.00 | F/L | 92-438 | [»] | |
4XLP | X-ray | 4.00 | F/L | 92-438 | [»] | |
4XLQ | X-ray | 4.60 | F/L | 92-438 | [»] | |
4XLR | X-ray | 4.30 | F/L | 92-438 | [»] | |
4XLS | X-ray | 4.01 | F/L | 92-438 | [»] | |
5TJG | X-ray | 2.60 | F | 92-438 | [»] | |
SMRi | Q9EZJ8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-49014N |
IntActi | Q9EZJ8, 1 interactor |
Miscellaneous databases
EvolutionaryTracei | Q9EZJ8 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
HAMAPi | MF_00963, Sigma70_RpoD_SigA, 1 hit |
InterProi | View protein in InterPro IPR014284, RNA_pol_sigma-70_dom IPR000943, RNA_pol_sigma70 IPR009042, RNA_pol_sigma70_r1_2 IPR007627, RNA_pol_sigma70_r2 IPR007624, RNA_pol_sigma70_r3 IPR007630, RNA_pol_sigma70_r4 IPR013325, RNA_pol_sigma_r2 IPR013324, RNA_pol_sigma_r3/r4-like IPR012760, RNA_pol_sigma_RpoD_C IPR028630, Sigma70_RpoD IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00140, Sigma70_r1_2, 1 hit PF04542, Sigma70_r2, 1 hit PF04539, Sigma70_r3, 1 hit PF04545, Sigma70_r4, 1 hit |
PRINTSi | PR00046, SIGMA70FCT |
SUPFAMi | SSF88659, SSF88659, 2 hits SSF88946, SSF88946, 1 hit |
TIGRFAMsi | TIGR02393, RpoD_Cterm, 1 hit TIGR02937, sigma70-ECF, 1 hit |
PROSITEi | View protein in PROSITE PS00716, SIGMA70_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SIGA_THEAQ | |
Accessioni | Q9EZJ8Primary (citable) accession number: Q9EZJ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 24, 2013 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families