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Entry version 78 (11 Dec 2019)
Sequence version 1 (01 Mar 2001)
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Protein

Leukotoxin

Gene

lktA

Organism
Mannheimia haemolytica (Pasteurella haemolytica)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca2+ and lysis of the host cell (By similarity). This leukotoxin is a major contributor to the pathogenesis of lung injury in bovine pneumonic pasteurellosis. It has also weak hemolytic activity.By similarity

Miscellaneous

The lktCABD operon has a complex mosaic structure that has been derived by extensive inter- and intraspecies horizontal DNA transfer and intragenic recombination events.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionToxin
Biological processCytolysis, Hemolysis, Virulence
LigandCalcium

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Leukotoxin
Short name:
Lkt
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:lktA
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMannheimia haemolytica (Pasteurella haemolytica)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri75985 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeMannheimia

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei229 – 249HelicalSequence analysisAdd BLAST21
Transmembranei297 – 317HelicalSequence analysisAdd BLAST21
Transmembranei359 – 379HelicalSequence analysisAdd BLAST21
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Secreted

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001962211 – 953LeukotoxinAdd BLAST953

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Acylated by LktC. The toxin only becomes active when modified (By similarity).By similarity

Keywords - PTMi

Lipoprotein

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q9EV30

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9EV30

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati715 – 732Hemolysin-type calcium-binding 1Add BLAST18
Repeati733 – 750Hemolysin-type calcium-binding 2Add BLAST18
Repeati751 – 768Hemolysin-type calcium-binding 3Add BLAST18
Repeati769 – 786Hemolysin-type calcium-binding 4Add BLAST18
Repeati789 – 806Hemolysin-type calcium-binding 5Add BLAST18

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The transmembrane domains are believed to be involved in pore formation in target cells.By similarity
The Gly-rich region is probably involved in calcium binding, which is required for target cell-binding and cytolytic activity.By similarity
The C-terminal domain contains an export signal that is recognized by the ABC transporter complex LktBD.By similarity

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.150.10.10, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR018511 Hemolysin-typ_Ca-bd_CS
IPR001343 Hemolysn_Ca-bd
IPR013550 RTX_C
IPR018504 RTX_N
IPR003995 RTX_toxin_determinant-A
IPR011049 Serralysin-like_metalloprot_C

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00353 HemolysinCabind, 3 hits
PF02382 RTX, 1 hit
PF08339 RTX_C, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01488 RTXTOXINA

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51120 SSF51120, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00330 HEMOLYSIN_CALCIUM, 4 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9EV30-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGTRLTTLSN GLKNTLTATK SGLHKAGQSL TQAGSSLKTG AKKIILYIPQ
60 70 80 90 100
NYQYDTEQGN GLQDLVKAAE ELGIEVQREE RNDIATAQTS LGTIQTAIGL
110 120 130 140 150
TERGIVLSAP QIDKLLQKTK AGQALGSAES IVQNANKAKT VLSGIQSILG
160 170 180 190 200
SVLAGMDLDE ALQNNSNQHA LAKAGLELTN SLIENIANSV KTLDEFGEQI
210 220 230 240 250
SQFGSKLQNI KGLGTLGDKL KNIGGLDKAG LGLDVISGLL SGATAALVLA
260 270 280 290 300
DKNASTAKKV GAGFELANQV VGNITKAVSS YILAQRVAAG LSSTGPVAAL
310 320 330 340 350
IASTVSLAIS PLAFAGIADK FNHAKSLESY AERFKKLGYD GDNLLAEYQR
360 370 380 390 400
GTGTIDASVT AINTALAAIA GGVSAAAAGS VIASPIALLV SGITGVISTI
410 420 430 440 450
LQYSKQAMFE HVANKIHNKI VEWEKNNQGK NYFENGYDAR YLANLQDNMK
460 470 480 490 500
FLLNLNKELQ AERVIAITQQ QWDSNIGDLA GISRLGEKVI SGKAYVDAFE
510 520 530 540 550
EGKHIKADKL VQLDSAKGII DVSNTGEAKT QHILFRTPLL TPGTEKRERV
560 570 580 590 600
QTGKYEYITK LNINRVDSWQ IKDGAASSTF DLTNVVQRIG IELDHAENVT
610 620 630 640 650
KTKETKIVAK LGAGDDNVFV GSGTTEIDGG EGYDRVHYSR GNYGALTIDA
660 670 680 690 700
TKETVQGSYT VNRFVETGKA LHEVTSTHTA LVGSREEKIE YRHSNNRQHA
710 720 730 740 750
GYYTKDTLTT IEEIIGTSHN DIFRGSKFND AFQGGDGVDT IDGNDGNDRL
760 770 780 790 800
FGGKGDDIID GGNGDDFIDG GKGNDLLHGG RGDDIFVHRK GDGNDIISDS
810 820 830 840 850
DGNDKLSFSD SNLKDLTFEK VKYNLVITNS NKEKVTIQNW FREADLAKEV
860 870 880 890 900
HNYKATADEK IEEIIGQNGE RITSKQVDDL IEKGNGKITQ DELSKAVDNY
910 920 930 940 950
ELLKHSKNVT NSLDKLISSV SSFTSSNDSR NVLVAPASML DQSLSSLQFA

RAA
Length:953
Mass (Da):102,044
Last modified:March 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4E8F11490479A69A
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF314512 Genomic DNA Translation: AAG40296.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314512 Genomic DNA Translation: AAG40296.1

3D structure databases

SMRiQ9EV30
ModBaseiSearch...

Proteomic databases

PRIDEiQ9EV30

Family and domain databases

Gene3Di2.150.10.10, 2 hits
InterProiView protein in InterPro
IPR018511 Hemolysin-typ_Ca-bd_CS
IPR001343 Hemolysn_Ca-bd
IPR013550 RTX_C
IPR018504 RTX_N
IPR003995 RTX_toxin_determinant-A
IPR011049 Serralysin-like_metalloprot_C
PfamiView protein in Pfam
PF00353 HemolysinCabind, 3 hits
PF02382 RTX, 1 hit
PF08339 RTX_C, 1 hit
PRINTSiPR01488 RTXTOXINA
SUPFAMiSSF51120 SSF51120, 1 hit
PROSITEiView protein in PROSITE
PS00330 HEMOLYSIN_CALCIUM, 4 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiLKA2A_MANHA
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9EV30
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2001
Last modified: December 11, 2019
This is version 78 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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