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Protein

Junctophilin-2

Gene

Jph2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH2 is necessary for proper intracellular Ca2+ signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Junctophilin-2
Short name:
JP-2
Alternative name(s):
Junctophilin type 2
Gene namesi
Name:Jph2
Synonyms:Jp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1891496 Jph2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 674CytoplasmicSequence analysisAdd BLAST674
Transmembranei675 – 695Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Null mice possess cardiomyocytes with deficiences in the junctional membrane complexes and have abnormal Ca2+ transients. Mice die from cardiac arrest at E10.5.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598481 – 696Junctophilin-2Add BLAST696

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineBy similarity1
Modified residuei165PhosphoserineBy similarity1
Modified residuei440PhosphoserineBy similarity1
Modified residuei442PhosphoserineBy similarity1
Modified residuei462PhosphoserineCombined sources1
Modified residuei470PhosphothreonineCombined sources1
Modified residuei479PhosphoserineCombined sources1
Modified residuei483PhosphothreonineCombined sources1
Modified residuei527PhosphoserineBy similarity1
Modified residuei533PhosphoserineBy similarity1
Modified residuei593PhosphoserineCombined sources1
Modified residuei597PhosphoserineCombined sources1
Modified residuei613PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9ET78
PaxDbiQ9ET78
PeptideAtlasiQ9ET78
PRIDEiQ9ET78

PTM databases

iPTMnetiQ9ET78
PhosphoSitePlusiQ9ET78

Expressioni

Tissue specificityi

Abundantly expressed in skeletal muscle and heart. Weak expression in stomach and lung.1 Publication

Developmental stagei

At E9.5, it is expressed in the periphery of cardiac myocytes in the looped cardiac tube of the developing heart. In skeletal muscle, expression increases during muscle maturation.

Gene expression databases

BgeeiENSMUSG00000017817 Expressed in 107 organ(s), highest expression level in cardiac ventricle
CleanExiMM_JPH2
GenevisibleiQ9ET78 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TRPC3G1U2U34EBI-8350389,EBI-10055313From Oryctolagus cuniculus.

Protein-protein interaction databases

BioGridi208526, 1 interactor
IntActiQ9ET78, 5 interactors
MINTiQ9ET78
STRINGi10090.ENSMUSP00000017961

Structurei

3D structure databases

ProteinModelPortaliQ9ET78
SMRiQ9ET78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 36MORN 1Add BLAST23
Repeati38 – 59MORN 2Add BLAST22
Repeati60 – 79MORN 3Add BLAST20
Repeati82 – 104MORN 4Add BLAST23
Repeati106 – 128MORN 5Add BLAST23
Repeati129 – 151MORN 6Add BLAST23
Repeati285 – 307MORN 7Add BLAST23
Repeati308 – 330MORN 8Add BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 142Gly-richAdd BLAST140
Compositional biasi367 – 402Ala-richAdd BLAST36

Domaini

The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, by interacting with phospholipids. Has affinity for phosphatidylserine, and phosphorylated phosphatidylinositols including PtdIns3P, PtdIns4P, PtdIns5P, PtdIns(3,5)P2 and PtdIns(3,4,5)P3 (By similarity).By similarity

Sequence similaritiesi

Belongs to the junctophilin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0231 Eukaryota
COG4642 LUCA
GeneTreeiENSGT00730000110639
HOGENOMiHOG000264244
HOVERGENiHBG031648
InParanoidiQ9ET78
KOiK19530
OMAiTIMICMV
OrthoDBiEOG091G03WX
PhylomeDBiQ9ET78
TreeFamiTF317210

Family and domain databases

InterProiView protein in InterPro
IPR017191 Junctophilin
IPR003409 MORN
PANTHERiPTHR23085 PTHR23085, 1 hit
PfamiView protein in Pfam
PF02493 MORN, 8 hits
PIRSFiPIRSF037387 Junctophilin, 1 hit
SMARTiView protein in SMART
SM00698 MORN, 6 hits

Sequencei

Sequence statusi: Complete.

Q9ET78-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGGRFDFDD GGAYCGGWEG GKAHGHGLCT GPKGQGEYSG SWNFGFEVAG
60 70 80 90 100
VYTWPSGNTF EGYWSQGKRH GLGIETKGRW LYKGEWTHGF KGRYGIRQST
110 120 130 140 150
NSGAKYEGTW NNGLQDGYGT ETYADGGTYQ GQFTNGMRHG YGVRQSVPYG
160 170 180 190 200
MAVVVRSPLR TSLSSLRSEH SNGTVAPDSP AADGPMLPSP PVPRGGFALT
210 220 230 240 250
LLATAEAARP QGLFTRGTLL GRLRRSESRT SLGSQRSRLS FLKSELSSGA
260 270 280 290 300
SDAASTGSLA EGAEGPDDAA APFDADIDAT TTETYMGEWK NDKRSGFGVS
310 320 330 340 350
ERSSGLRYEG EWLDNLRHGY GRTTLPDGHR EEGKYRHNVL VKGTKRRVLP
360 370 380 390 400
LKSSKVRQKV EHGVEGAQRA AAIARQKAEI AASRTSHAKA KAEAAEQAAL
410 420 430 440 450
AANQESNIAR TLAKELAPDF YQPGPEYQKR RLLQEILENS ESLLEPPERG
460 470 480 490 500
LGTGLPERPR ESPQLHERET PQPEGGPPSP AGTPPQPKRP RPGASKDGLL
510 520 530 540 550
SPGSWNGEPG GEGSRPATPS DGAGRRSPAR PASEHMAIEA LQPPPAPSQE
560 570 580 590 600
PEVAMYRGYH SYAVRTGPPE PPPLEDEQEP EPEPEPEVRR SDSAPPSPVS
610 620 630 640 650
ATVPEEEPPA PRSPVPAKQA TLEPKPIVPK AEPKAKARKT EARGLSKAGA
660 670 680 690
KKKGRKEVAQ AKEAEVEVEE VPNTVLICMV ILLNIGLAIL FVHLLT
Length:696
Mass (Da):74,692
Last modified:January 17, 2003 - v2
Checksum:i346BA284E076A7FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024447 mRNA Translation: BAB12044.1
AB024448 Genomic DNA Translation: BAB12045.1
AK157145 mRNA Translation: BAE33978.1
AL589876 Genomic DNA Translation: CAM13817.1
CCDSiCCDS17008.1
RefSeqiNP_001192005.1, NM_001205076.1
NP_067541.1, NM_021566.2
XP_006500035.1, XM_006499972.2
UniGeneiMm.34459

Genome annotation databases

EnsembliENSMUST00000017961; ENSMUSP00000017961; ENSMUSG00000017817
ENSMUST00000109425; ENSMUSP00000105052; ENSMUSG00000017817
GeneIDi59091
KEGGimmu:59091
UCSCiuc008nsp.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024447 mRNA Translation: BAB12044.1
AB024448 Genomic DNA Translation: BAB12045.1
AK157145 mRNA Translation: BAE33978.1
AL589876 Genomic DNA Translation: CAM13817.1
CCDSiCCDS17008.1
RefSeqiNP_001192005.1, NM_001205076.1
NP_067541.1, NM_021566.2
XP_006500035.1, XM_006499972.2
UniGeneiMm.34459

3D structure databases

ProteinModelPortaliQ9ET78
SMRiQ9ET78
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208526, 1 interactor
IntActiQ9ET78, 5 interactors
MINTiQ9ET78
STRINGi10090.ENSMUSP00000017961

PTM databases

iPTMnetiQ9ET78
PhosphoSitePlusiQ9ET78

Proteomic databases

MaxQBiQ9ET78
PaxDbiQ9ET78
PeptideAtlasiQ9ET78
PRIDEiQ9ET78

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017961; ENSMUSP00000017961; ENSMUSG00000017817
ENSMUST00000109425; ENSMUSP00000105052; ENSMUSG00000017817
GeneIDi59091
KEGGimmu:59091
UCSCiuc008nsp.2 mouse

Organism-specific databases

CTDi57158
MGIiMGI:1891496 Jph2

Phylogenomic databases

eggNOGiKOG0231 Eukaryota
COG4642 LUCA
GeneTreeiENSGT00730000110639
HOGENOMiHOG000264244
HOVERGENiHBG031648
InParanoidiQ9ET78
KOiK19530
OMAiTIMICMV
OrthoDBiEOG091G03WX
PhylomeDBiQ9ET78
TreeFamiTF317210

Miscellaneous databases

PROiPR:Q9ET78
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017817 Expressed in 107 organ(s), highest expression level in cardiac ventricle
CleanExiMM_JPH2
GenevisibleiQ9ET78 MM

Family and domain databases

InterProiView protein in InterPro
IPR017191 Junctophilin
IPR003409 MORN
PANTHERiPTHR23085 PTHR23085, 1 hit
PfamiView protein in Pfam
PF02493 MORN, 8 hits
PIRSFiPIRSF037387 Junctophilin, 1 hit
SMARTiView protein in SMART
SM00698 MORN, 6 hits
ProtoNetiSearch...

Entry informationi

Entry nameiJPH2_MOUSE
AccessioniPrimary (citable) accession number: Q9ET78
Secondary accession number(s): Q3U077, Q9ET79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: January 17, 2003
Last modified: November 7, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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