Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycogen phosphorylase, liver form

Gene

Pygl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity).By similarity

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

Activity regulationi

Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76AMPBy similarity1
Sitei109Involved in the association of subunitsBy similarity1
Sitei143Involved in the association of subunitsBy similarity1
Sitei156May be involved in allosteric controlBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 5-phosphoribose 1-diphosphate biosynthetic process Source: MGI
  • glucose homeostasis Source: MGI
  • glycogen catabolic process Source: MGI
  • glycogen metabolic process Source: MGI
  • necroptotic process Source: MGI
  • response to bacterium Source: MGI

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandNucleotide-binding, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-70221 Glycogen breakdown (glycogenolysis)

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase, liver form (EC:2.4.1.1)
Gene namesi
Name:Pygl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:97829 Pygl

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3008

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001885252 – 850Glycogen phosphorylase, liver formAdd BLAST849

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei15Phosphoserine; by PHK; in form phosphorylase ABy similarity1
Modified residuei364N6-succinyllysineCombined sources1
Modified residuei524PhosphoserineCombined sources1
Modified residuei527PhosphoserineCombined sources1
Modified residuei561PhosphoserineBy similarity1
Modified residuei639PhosphoserineBy similarity1
Modified residuei681N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9ET01
MaxQBiQ9ET01
PaxDbiQ9ET01
PeptideAtlasiQ9ET01
PRIDEiQ9ET01

PTM databases

iPTMnetiQ9ET01
PhosphoSitePlusiQ9ET01
SwissPalmiQ9ET01

Expressioni

Gene expression databases

BgeeiENSMUSG00000021069 Expressed in 260 organ(s), highest expression level in brown adipose tissue
CleanExiMM_PYGL
ExpressionAtlasiQ9ET01 baseline and differential
GenevisibleiQ9ET01 MM

Interactioni

Subunit structurei

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Interacts with PPP1R3B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi225292, 1 interactor
IntActiQ9ET01, 5 interactors
MINTiQ9ET01
STRINGi10090.ENSMUSP00000071231

Chemistry databases

BindingDBiQ9ET01

Structurei

3D structure databases

ProteinModelPortaliQ9ET01
SMRiQ9ET01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
GeneTreeiENSGT00390000016886
HOGENOMiHOG000278444
HOVERGENiHBG006848
InParanoidiQ9ET01
KOiK00688
OrthoDBiEOG091G03RB
TreeFamiTF300309

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9ET01-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKPLTDQEK RRQISIRGIV GVENVAELKK GFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYYFALAHTV RDHLVGRWIR TQQHYYDKCP KRVYYLSLEF YMGRTLQNTM
110 120 130 140 150
INLGLQNACD EAIYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEYGIFNQK IREGWQVEEA DDWLRHGNPW EKARPEFMLP
210 220 230 240 250
VHFYGRVEHT QTGTKWVDTQ VVLALPYDTP VPGYMNNTVN TMRLWSARAP
260 270 280 290 300
NDFNLQDFNV GDYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDVIR RFKASKFGSK DGMGTVFDAF PDQVAIQLND THPALAIPEL
360 370 380 390 400
MRIFVDIEKL PWAKAWEITK KTFAYTNHTV LPEALERWPV ELVEKLLPRH
410 420 430 440 450
LEIIYEINQK HLDRIVALFP KDISRMRRMS LIEEEGGKRI NMAHLCIVGC
460 470 480 490 500
HAVNGVAKIH SDIVKTQVFK DFSELEPDKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
ADLIAEKIGE DYVKDLSQLT KLHSFVSDDI FLREIAKVKQ ENKLKFSQFL
560 570 580 590 600
EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVITMYNR IKKDPKKFFV
610 620 630 640 650
PRTVIIGGKA APGYHMAKMI IKLITSVAEV VNNDPMVGSK LKVIFLENYR
660 670 680 690 700
VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEENLF IFGMRVDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF
760 770 780 790 800
FSPNQPDLFK DIINMLFYHD RFKVFADYEA YVKCQEKVSQ LYMNQKAWNT
810 820 830 840 850
MVLKNIAASG KFSSDRTIKE YAKDIWNMEP SDLKISLSNE SSNGVSANGK
Length:850
Mass (Da):97,463
Last modified:July 27, 2011 - v4
Checksum:iC4BFF6A9AA4E181E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UEJ6Q3UEJ6_MOUSE
Alpha-1,4 glucan phosphorylase
Pygl
759Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198M → V in AAG00588 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288783 mRNA Translation: AAG00588.1
AK140321 mRNA Translation: BAE24332.1
AK145989 mRNA Translation: BAE26811.1
CCDSiCCDS25957.1
RefSeqiNP_573461.2, NM_133198.2
UniGeneiMm.256926
Mm.447796

Genome annotation databases

EnsembliENSMUST00000071250; ENSMUSP00000071231; ENSMUSG00000021069
GeneIDi110095
KEGGimmu:110095
UCSCiuc007ntm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288783 mRNA Translation: AAG00588.1
AK140321 mRNA Translation: BAE24332.1
AK145989 mRNA Translation: BAE26811.1
CCDSiCCDS25957.1
RefSeqiNP_573461.2, NM_133198.2
UniGeneiMm.256926
Mm.447796

3D structure databases

ProteinModelPortaliQ9ET01
SMRiQ9ET01
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225292, 1 interactor
IntActiQ9ET01, 5 interactors
MINTiQ9ET01
STRINGi10090.ENSMUSP00000071231

Chemistry databases

BindingDBiQ9ET01
ChEMBLiCHEMBL3008

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

PTM databases

iPTMnetiQ9ET01
PhosphoSitePlusiQ9ET01
SwissPalmiQ9ET01

Proteomic databases

EPDiQ9ET01
MaxQBiQ9ET01
PaxDbiQ9ET01
PeptideAtlasiQ9ET01
PRIDEiQ9ET01

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071250; ENSMUSP00000071231; ENSMUSG00000021069
GeneIDi110095
KEGGimmu:110095
UCSCiuc007ntm.2 mouse

Organism-specific databases

CTDi5836
MGIiMGI:97829 Pygl

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
GeneTreeiENSGT00390000016886
HOGENOMiHOG000278444
HOVERGENiHBG006848
InParanoidiQ9ET01
KOiK00688
OrthoDBiEOG091G03RB
TreeFamiTF300309

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-70221 Glycogen breakdown (glycogenolysis)

Miscellaneous databases

ChiTaRSiPygl mouse
PROiPR:Q9ET01
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021069 Expressed in 260 organ(s), highest expression level in brown adipose tissue
CleanExiMM_PYGL
ExpressionAtlasiQ9ET01 baseline and differential
GenevisibleiQ9ET01 MM

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPYGL_MOUSE
AccessioniPrimary (citable) accession number: Q9ET01
Secondary accession number(s): Q3UKJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 155 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again