UniProtKB - Q9ET01 (PYGL_MOUSE)
Protein
Glycogen phosphorylase, liver form
Gene
Pygl
Organism
Mus musculus (Mouse)
Status
Functioni
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity).By similarity
Catalytic activityi
- EC:2.4.1.1
Cofactori
pyridoxal 5'-phosphateBy similarity
Activity regulationi
Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 76 | AMPBy similarity | 1 | |
Sitei | 109 | Involved in the association of subunitsBy similarity | 1 | |
Sitei | 143 | Involved in the association of subunitsBy similarity | 1 | |
Sitei | 156 | May be involved in allosteric controlBy similarity | 1 |
GO - Molecular functioni
- AMP binding Source: MGI
- ATP binding Source: MGI
- bile acid binding Source: MGI
- carbohydrate binding Source: MGI
- drug binding Source: Ensembl
- glucose binding Source: MGI
- glycogen phosphorylase activity Source: MGI
- identical protein binding Source: MGI
- linear malto-oligosaccharide phosphorylase activity Source: UniProtKB-EC
- purine nucleobase binding Source: MGI
- pyridoxal phosphate binding Source: GO_Central
- SHG alpha-glucan phosphorylase activity Source: UniProtKB-EC
- vitamin binding Source: MGI
GO - Biological processi
- 5-phosphoribose 1-diphosphate biosynthetic process Source: MGI
- glucose homeostasis Source: MGI
- glycogen catabolic process Source: MGI
- glycogen metabolic process Source: MGI
- necroptotic process Source: MGI
- response to bacterium Source: MGI
Keywordsi
Molecular function | Allosteric enzyme, Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen metabolism |
Ligand | Nucleotide-binding, Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation R-MMU-70221, Glycogen breakdown (glycogenolysis) |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Pygl |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97829, Pygl |
Subcellular locationi
Other locations
- cytoplasm Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000188525 | 2 – 850 | Glycogen phosphorylase, liver formAdd BLAST | 849 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 15 | Phosphoserine; by PHK; in form phosphorylase ABy similarity | 1 | |
Modified residuei | 364 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 524 | PhosphoserineCombined sources | 1 | |
Modified residuei | 527 | PhosphoserineCombined sources | 1 | |
Modified residuei | 561 | PhosphoserineBy similarity | 1 | |
Modified residuei | 639 | PhosphoserineBy similarity | 1 | |
Modified residuei | 681 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Post-translational modificationi
Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3937 |
EPDi | Q9ET01 |
jPOSTi | Q9ET01 |
MaxQBi | Q9ET01 |
PaxDbi | Q9ET01 |
PeptideAtlasi | Q9ET01 |
PRIDEi | Q9ET01 |
PTM databases
iPTMneti | Q9ET01 |
PhosphoSitePlusi | Q9ET01 |
SwissPalmi | Q9ET01 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021069, Expressed in brown adipose tissue and 278 other tissues |
ExpressionAtlasi | Q9ET01, baseline and differential |
Genevisiblei | Q9ET01, MM |
Interactioni
Subunit structurei
Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.
Interacts with PPP1R3B (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 225292, 11 interactors |
IntActi | Q9ET01, 5 interactors |
MINTi | Q9ET01 |
STRINGi | 10090.ENSMUSP00000071231 |
Chemistry databases
BindingDBi | Q9ET01 |
Miscellaneous databases
RNActi | Q9ET01, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycogen phosphorylase family.Curated
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
GeneTreei | ENSGT00950000183148 |
HOGENOMi | CLU_010198_1_0_1 |
InParanoidi | Q9ET01 |
OrthoDBi | 240595at2759 |
TreeFami | TF300309 |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9ET01-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKPLTDQEK RRQISIRGIV GVENVAELKK GFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYYFALAHTV RDHLVGRWIR TQQHYYDKCP KRVYYLSLEF YMGRTLQNTM
110 120 130 140 150
INLGLQNACD EAIYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEYGIFNQK IREGWQVEEA DDWLRHGNPW EKARPEFMLP
210 220 230 240 250
VHFYGRVEHT QTGTKWVDTQ VVLALPYDTP VPGYMNNTVN TMRLWSARAP
260 270 280 290 300
NDFNLQDFNV GDYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDVIR RFKASKFGSK DGMGTVFDAF PDQVAIQLND THPALAIPEL
360 370 380 390 400
MRIFVDIEKL PWAKAWEITK KTFAYTNHTV LPEALERWPV ELVEKLLPRH
410 420 430 440 450
LEIIYEINQK HLDRIVALFP KDISRMRRMS LIEEEGGKRI NMAHLCIVGC
460 470 480 490 500
HAVNGVAKIH SDIVKTQVFK DFSELEPDKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
ADLIAEKIGE DYVKDLSQLT KLHSFVSDDI FLREIAKVKQ ENKLKFSQFL
560 570 580 590 600
EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVITMYNR IKKDPKKFFV
610 620 630 640 650
PRTVIIGGKA APGYHMAKMI IKLITSVAEV VNNDPMVGSK LKVIFLENYR
660 670 680 690 700
VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEENLF IFGMRVDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF
760 770 780 790 800
FSPNQPDLFK DIINMLFYHD RFKVFADYEA YVKCQEKVSQ LYMNQKAWNT
810 820 830 840 850
MVLKNIAASG KFSSDRTIKE YAKDIWNMEP SDLKISLSNE SSNGVSANGK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ3UEJ6 | Q3UEJ6_MOUSE | Alpha-1,4 glucan phosphorylase | Pygl | 759 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 198 | M → V in AAG00588 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF288783 mRNA Translation: AAG00588.1 AK140321 mRNA Translation: BAE24332.1 AK145989 mRNA Translation: BAE26811.1 |
CCDSi | CCDS25957.1 |
RefSeqi | NP_573461.2, NM_133198.2 |
Genome annotation databases
Ensembli | ENSMUST00000071250; ENSMUSP00000071231; ENSMUSG00000021069 |
GeneIDi | 110095 |
KEGGi | mmu:110095 |
UCSCi | uc007ntm.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF288783 mRNA Translation: AAG00588.1 AK140321 mRNA Translation: BAE24332.1 AK145989 mRNA Translation: BAE26811.1 |
CCDSi | CCDS25957.1 |
RefSeqi | NP_573461.2, NM_133198.2 |
3D structure databases
SMRi | Q9ET01 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225292, 11 interactors |
IntActi | Q9ET01, 5 interactors |
MINTi | Q9ET01 |
STRINGi | 10090.ENSMUSP00000071231 |
Chemistry databases
BindingDBi | Q9ET01 |
ChEMBLi | CHEMBL3008 |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
PTM databases
iPTMneti | Q9ET01 |
PhosphoSitePlusi | Q9ET01 |
SwissPalmi | Q9ET01 |
Proteomic databases
CPTACi | non-CPTAC-3937 |
EPDi | Q9ET01 |
jPOSTi | Q9ET01 |
MaxQBi | Q9ET01 |
PaxDbi | Q9ET01 |
PeptideAtlasi | Q9ET01 |
PRIDEi | Q9ET01 |
Protocols and materials databases
Antibodypediai | 25, 289 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000071250; ENSMUSP00000071231; ENSMUSG00000021069 |
GeneIDi | 110095 |
KEGGi | mmu:110095 |
UCSCi | uc007ntm.2, mouse |
Organism-specific databases
CTDi | 5836 |
MGIi | MGI:97829, Pygl |
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
GeneTreei | ENSGT00950000183148 |
HOGENOMi | CLU_010198_1_0_1 |
InParanoidi | Q9ET01 |
OrthoDBi | 240595at2759 |
TreeFami | TF300309 |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation R-MMU-70221, Glycogen breakdown (glycogenolysis) |
Miscellaneous databases
BioGRID-ORCSi | 110095, 4 hits in 19 CRISPR screens |
ChiTaRSi | Pygl, mouse |
PROi | PR:Q9ET01 |
RNActi | Q9ET01, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021069, Expressed in brown adipose tissue and 278 other tissues |
ExpressionAtlasi | Q9ET01, baseline and differential |
Genevisiblei | Q9ET01, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PYGL_MOUSE | |
Accessioni | Q9ET01Primary (citable) accession number: Q9ET01 Secondary accession number(s): Q3UKJ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2002 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 169 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families