UniProtKB - Q9ESW4 (AGK_MOUSE)
Protein
Acylglycerol kinase, mitochondrial
Gene
Agk
Organism
Mus musculus (Mouse)
Status
Functioni
Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15252046). Phosphorylates ceramide but not sphingosine (PubMed:15252046). Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol (PubMed:18004883). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (By similarity). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (By similarity). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (By similarity). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (By similarity).By similarity2 Publications
Caution
Was originally (PubMed:15252046) thought to have ceramide kinase activity. Such activity is however unlikely in vivo.1 Publication
Catalytic activityi
- EC:2.7.1.941 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:2.7.1.1072 PublicationsThis reaction proceeds in the forward2 Publications direction.
- EC:2.7.1.1381 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + ATP = 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Mg2+1 Publication
Activity regulationi
Both the ceramide and diacylglycerol kinase activities are inhibited by sphingosine and stimulated by cardiolipin (PubMed:15252046). Both activities are stimulated by calcium when magnesium concentrations are low but inhibited by calcium when magnesium concentrations are high (PubMed:15252046).1 Publication
Kineticsi
- KM=45 µM for diacylglycerol1 Publication
- KM=34 µM for ceramide1 Publication
- Vmax=159 nmol/min/mg enzyme with dioeoylglycerol as substrate1 Publication
- Vmax=37 nmol/min/mg enzyme with ceramide as substrate1 Publication
- Vmax=23.6 nmol/min/mg enzyme with 1,2-dioleoylglycerol as substrate1 Publication
- Vmax=10.21 nmol/min/mg enzyme with 2,3-dioleoylglycerol as substrate1 Publication
: glycerolipid metabolism Pathwayi
This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.
GO - Molecular functioni
- acylglycerol kinase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- ceramide kinase activity Source: MGI
- D-erythro-sphingosine kinase activity Source: GO_Central
- diacylglycerol kinase activity Source: UniProtKB
- dihydroceramide kinase activity Source: UniProtKB-EC
- lipid kinase activity Source: MGI
- NAD+ kinase activity Source: InterPro
GO - Biological processi
- ceramide biosynthetic process Source: MGI
- glycerolipid metabolic process Source: UniProtKB-UniPathway
- lipid phosphorylation Source: MGI
- phosphorylation Source: GO_Central
- protein insertion into mitochondrial inner membrane Source: UniProtKB
- sphingolipid metabolic process Source: GO_Central
- sphingosine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1483206, Glycerophospholipid biosynthesis |
SABIO-RKi | Q9ESW4 |
UniPathwayi | UPA00230 |
Chemistry databases
SwissLipidsi | SLP:000000885 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917173, Agk |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
- Mitochondrion intermembrane space By similarity
Note: Localizes in the mitochondrion intermembrane space, where it associates with the inner membrane. It is unclear whether the N-terminal hydrophobic region forms a transmembrane region or associates with the membrane without crossing it.By similarity
Mitochondrion
- integral component of mitochondrial inner membrane Source: UniProtKB
- mitochondrial inner membrane Source: UniProtKB
- mitochondrial intermembrane space Source: UniProtKB
- mitochondrial membrane Source: UniProtKB
- mitochondrion Source: MGI
- TIM22 mitochondrial import inner membrane insertion complex Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252381 | 1 – 421 | Acylglycerol kinase, mitochondrialAdd BLAST | 421 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9ESW4 |
jPOSTi | Q9ESW4 |
PaxDbi | Q9ESW4 |
PeptideAtlasi | Q9ESW4 |
PRIDEi | Q9ESW4 |
PTM databases
iPTMneti | Q9ESW4 |
PhosphoSitePlusi | Q9ESW4 |
SwissPalmi | Q9ESW4 |
Expressioni
Tissue specificityi
Ubiquitously expressed.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000029916, Expressed in zygote and 104 other tissues |
ExpressionAtlasi | Q9ESW4, baseline and differential |
Genevisiblei | Q9ESW4, MM |
Interactioni
Subunit structurei
Component of the TIM22 complex, which core is composed of TIMM22, associated with TIMM10 (TIMM10A and/or TIMM10B), TIMM9, AGK and TIMM29.
By similarityProtein-protein interaction databases
BioGRIDi | 213759, 3 interactors |
IntActi | Q9ESW4, 2 interactors |
MINTi | Q9ESW4 |
STRINGi | 10090.ENSMUSP00000031977 |
Miscellaneous databases
RNActi | Q9ESW4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 58 – 199 | DAGKcPROSITE-ProRule annotationAdd BLAST | 142 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 31 | HydrophobicBy similarityAdd BLAST | 17 |
Sequence similaritiesi
Belongs to the AGK family.Curated
Phylogenomic databases
eggNOGi | KOG4435, Eukaryota |
GeneTreei | ENSGT00940000154961 |
HOGENOMi | CLU_042458_0_0_1 |
InParanoidi | Q9ESW4 |
OMAi | KEWPQIH |
OrthoDBi | 1312024at2759 |
PhylomeDBi | Q9ESW4 |
TreeFami | TF320485 |
Family and domain databases
Gene3Di | 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR001206, Diacylglycerol_kinase_cat_dom IPR016064, NAD/diacylglycerol_kinase_sf |
Pfami | View protein in Pfam PF00781, DAGK_cat, 1 hit |
SMARTi | View protein in SMART SM00046, DAGKc, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9ESW4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTAFFKTLRN HWKKTTAGLC LLTWGGHWLY GKHCDNLLRR AACQEAQVFG
60 70 80 90 100
NQLIPPNAQV KKATVFLNPA ACKGKARTLF EKNAAPILHL SGMDVTVVKT
110 120 130 140 150
DYEGQAKKLL ELMESTDVII VAGGDGTLQE VVTGVLRRTD EATFSKIPIG
160 170 180 190 200
FIPLGQTSSL SHTLFAESGN KVQHITDATL AIVKGETVPL DVLQIKGEKE
210 220 230 240 250
QPVYAMTGLR WGSFRDAGVK VSKYWYLGPL KTKAAHFFST LQEWPQTHQA
260 270 280 290 300
SISYTGPRER PPIEPEETPP RPSLYRRILR RLASFWAQPQ DASSREVSPE
310 320 330 340 350
VWKDVQLSTI ELSITTRNTQ LDLMSKEDFM NICIEPDTVS KGDFIIIGSK
360 370 380 390 400
KVRDPGLRAA GTECLQASHC TLVLPEGTEG SFSIDSEEYE AMPVEVKLLP
410 420
RKLRFFCDPR KREQMLPSTS Q
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YU24 | A0A0J9YU24_MOUSE | Acylglycerol kinase, mitochondrial | Agk | 78 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020927 | 174 – 421 | Missing in isoform 2. 1 PublicationAdd BLAST | 248 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY772469 mRNA Translation: AAV38106.1 AJ401619 mRNA Translation: CAC06108.1 AK011715 mRNA Translation: BAB27796.1 AK076224 mRNA Translation: BAC36260.1 BC019145 mRNA Translation: AAH19145.1 BC093525 mRNA Translation: AAH93525.1 |
CCDSi | CCDS20027.1 [Q9ESW4-1] |
RefSeqi | NP_076027.1, NM_023538.2 [Q9ESW4-1] |
Genome annotation databases
Ensembli | ENSMUST00000031977; ENSMUSP00000031977; ENSMUSG00000029916 [Q9ESW4-1] |
GeneIDi | 69923 |
KEGGi | mmu:69923 |
UCSCi | uc009bmm.2, mouse [Q9ESW4-2] uc009bmn.2, mouse [Q9ESW4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY772469 mRNA Translation: AAV38106.1 AJ401619 mRNA Translation: CAC06108.1 AK011715 mRNA Translation: BAB27796.1 AK076224 mRNA Translation: BAC36260.1 BC019145 mRNA Translation: AAH19145.1 BC093525 mRNA Translation: AAH93525.1 |
CCDSi | CCDS20027.1 [Q9ESW4-1] |
RefSeqi | NP_076027.1, NM_023538.2 [Q9ESW4-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 213759, 3 interactors |
IntActi | Q9ESW4, 2 interactors |
MINTi | Q9ESW4 |
STRINGi | 10090.ENSMUSP00000031977 |
Chemistry databases
SwissLipidsi | SLP:000000885 |
PTM databases
iPTMneti | Q9ESW4 |
PhosphoSitePlusi | Q9ESW4 |
SwissPalmi | Q9ESW4 |
Proteomic databases
EPDi | Q9ESW4 |
jPOSTi | Q9ESW4 |
PaxDbi | Q9ESW4 |
PeptideAtlasi | Q9ESW4 |
PRIDEi | Q9ESW4 |
Protocols and materials databases
Antibodypediai | 18305, 191 antibodies |
DNASUi | 69923 |
Genome annotation databases
Ensembli | ENSMUST00000031977; ENSMUSP00000031977; ENSMUSG00000029916 [Q9ESW4-1] |
GeneIDi | 69923 |
KEGGi | mmu:69923 |
UCSCi | uc009bmm.2, mouse [Q9ESW4-2] uc009bmn.2, mouse [Q9ESW4-1] |
Organism-specific databases
CTDi | 55750 |
MGIi | MGI:1917173, Agk |
Phylogenomic databases
eggNOGi | KOG4435, Eukaryota |
GeneTreei | ENSGT00940000154961 |
HOGENOMi | CLU_042458_0_0_1 |
InParanoidi | Q9ESW4 |
OMAi | KEWPQIH |
OrthoDBi | 1312024at2759 |
PhylomeDBi | Q9ESW4 |
TreeFami | TF320485 |
Enzyme and pathway databases
UniPathwayi | UPA00230 |
Reactomei | R-MMU-1483206, Glycerophospholipid biosynthesis |
SABIO-RKi | Q9ESW4 |
Miscellaneous databases
BioGRID-ORCSi | 69923, 2 hits in 17 CRISPR screens |
ChiTaRSi | Agk, mouse |
PROi | PR:Q9ESW4 |
RNActi | Q9ESW4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029916, Expressed in zygote and 104 other tissues |
ExpressionAtlasi | Q9ESW4, baseline and differential |
Genevisiblei | Q9ESW4, MM |
Family and domain databases
Gene3Di | 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR001206, Diacylglycerol_kinase_cat_dom IPR016064, NAD/diacylglycerol_kinase_sf |
Pfami | View protein in Pfam PF00781, DAGK_cat, 1 hit |
SMARTi | View protein in SMART SM00046, DAGKc, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AGK_MOUSE | |
Accessioni | Q9ESW4Primary (citable) accession number: Q9ESW4 Secondary accession number(s): Q9D087 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families