Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bromodomain-containing protein 4

Gene

Brd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.2 Publications

Miscellaneous

Some specific inhibitors of Brd4 that prevent binding to acetylated histones by binding Asn-140 and Asn-434 are promising therapeutic molecules for the treatment of leukemias. JQ1, a thieno-triazolo-1,4-diazepine derivative, and I-BET, a benzodiazepine derivative, have been tested on tumors with success. Treatment with GSK1210151A (I-BET151, a I-BET derivative) has strong effets on mixed lineage leukemia and promotes myeloid differentiation and leukemia stem-cell depletion (PubMed:21814200, PubMed:21964340).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei140Acetylated histones1 Publication1
Binding sitei140InhibitorBy similarity1
Binding sitei434Acetylated histonesBy similarity1
Binding sitei434InhibitorBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Bromodomain-containing protein 4
Alternative name(s):
Mitotic chromosome-associated protein
Short name:
MCAP
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Brd4
Synonyms:Mcap
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1888520 Brd4

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Embryonic lethal. Embryos ndie shortly after implantation and are compromised in their ability to maintain an inner cell mass.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi139Y → A: No effect on acetylated histone binding. 1 Publication1
Mutagenesisi146I → A: No effect on acetylated histone binding. 1 Publication1
Mutagenesisi433Y → A: No effect on acetylated histone binding. 1 Publication1
Mutagenesisi440V → A: No effect on acetylated histone binding. 1 Publication1

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...
ChEMBLi
CHEMBL3085619

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002111841 – 1400Bromodomain-containing protein 4Add BLAST1400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei471PhosphoserineBy similarity1
Modified residuei485Phosphoserine; by CK2By similarity1
Modified residuei489Phosphoserine; by CK2By similarity1
Modified residuei493Phosphoserine; by CK2By similarity1
Modified residuei495PhosphoserineBy similarity1
Modified residuei499Phosphoserine; by CK2By similarity1
Modified residuei500Phosphoserine; by CK2By similarity1
Modified residuei504Phosphoserine; by CK2By similarity1
Cross-linki586Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei602PhosphoserineBy similarity1
Cross-linki646Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki695Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1053Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1147N6-acetyllysine; alternateBy similarity1
Cross-linki1147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki1147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei1153PhosphoserineBy similarity1
Modified residuei1162PhosphoserineBy similarity1
Cross-linki1233Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1237PhosphoserineBy similarity1
Modified residuei1240PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones. In absence of phosphorylation, BRD4 does not localize to p53/TP53 target gene promoters, phosphorylation promoting recruitment to p53/TP53 target promoters (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q9ESU6

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9ESU6

PRoteomics IDEntifications database

More...
PRIDEi
Q9ESU6

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q9ESU6

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q9ESU6

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000024002 Expressed in 305 organ(s), highest expression level in pituitary gland

CleanEx database of gene expression profiles

More...
CleanExi
MM_BRD4

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9ESU6 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q9ESU6 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with p53/TP53; the interaction is direct. Interacts with RELA (when acetylated at 'Lys-310'). Interacts (via NET domain) with WHSC1L1, JMJD6, CHD4, BICRA and ATAD5. The interaction with BICRA bridges BRD4 to the GBAF complex (By similarity). Interacts (via CTD region) with CDK9 and CCNT1, acting as an associated component of P-TEFb complex (PubMed:16109376).By similarity1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
RELAQ042066EBI-6260864,EBI-73886From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
208231, 1 interactor

CORUM comprehensive resource of mammalian protein complexes

More...
CORUMi
Q9ESU6

Database of interacting proteins

More...
DIPi
DIP-42181N

Protein interaction database and analysis system

More...
IntActi
Q9ESU6, 6 interactors

Molecular INTeraction database

More...
MINTi
Q9ESU6

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000113070

Chemistry databases

BindingDB database of measured binding affinities

More...
BindingDBi
Q9ESU6

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q9ESU6

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9ESU6

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q9ESU6

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini75 – 147Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini369 – 441Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini601 – 683NETPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni485 – 504NPS regionBy similarityAdd BLAST20
Regioni525 – 580BID regionBy similarityAdd BLAST56
Regioni1050 – 1400C-terminal (CTD) regionBy similarityAdd BLAST351

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi206 – 345Pro-richAdd BLAST140
Compositional biasi485 – 504Ser-richAdd BLAST20
Compositional biasi536 – 595Lys-richAdd BLAST60
Compositional biasi693 – 718Ser-richAdd BLAST26
Compositional biasi745 – 1226Pro-richAdd BLAST482
Compositional biasi929 – 1115Gln-richAdd BLAST187
Compositional biasi1319 – 1362Gln-richAdd BLAST44

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The 2 bromo domains mediate specific binding to acetylated histones via Asn-140 and Asn-434, respectively (PubMed:19828451). The exact combination of modified histone tails required to recruit BRD4 to target genes is still unclear. The first bromo domain has high affinity for acetylated histone H4 tail, whereas the second bromo domain recognizes multiply acetylated marks in histone H3 (By similarity).By similarity1 Publication
The NET domain mediates interaction with a number of chromatin proteins involved in transcription regulation (WHSC1L1, JMJD6, CHD4, GLTSCR1 and ATAD5).By similarity
The C-terminal (CTD) region mediates interaction and recruitment of CDK9 and CCNT1 subunits of the P-TEFb complex. It is also required for maintenance of higher-order chromatin structure (By similarity).By similarity

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1474 Eukaryota
COG5076 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000154549

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000231200

The HOVERGEN Database of Homologous Vertebrate Genes

More...
HOVERGENi
HBG004896

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q9ESU6

KEGG Orthology (KO)

More...
KOi
K11722

TreeFam database of animal gene trees

More...
TreeFami
TF317345

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.1270.220, 1 hit
1.20.920.10, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR031354 BRD4_CDT
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR027353 NET_dom
IPR038336 NET_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF17035 BET, 1 hit
PF17105 BRD4_CDT, 1 hit
PF00439 Bromodomain, 2 hits

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00503 BROMODOMAIN

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00297 BROMO, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47370 SSF47370, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00633 BROMODOMAIN_1, 1 hit
PS50014 BROMODOMAIN_2, 2 hits
PS51525 NET, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9ESU6-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSTESGPGTR LRNLPVMGDG LETSQMSTTQ AQAQPQPANA ASTNPPPPET
60 70 80 90 100
SNPNKPKRQT NQLQYLLRVV LKTLWKHQFA WPFQQPVDAV KLNLPDYYKI
110 120 130 140 150
IKTPMDMGTI KKRLENNYYW NAQECIQDFN TMFTNCYIYN KPGDDIVLMA
160 170 180 190 200
EALEKLFLQK INELPTEETE IMIVQAKGRG RGRKETGAAK PGVSTVPNTT
210 220 230 240 250
QASTSPQTQT PQQNPPPPVQ ATTHPFPAVT PDLIAQPPVM TMVPPQPLQT
260 270 280 290 300
PSPVPPQPPP PPAPVPQPVQ SHPPIIATTP QPVKTKKGVK RKADTTTPTT
310 320 330 340 350
IDPIHEPPSL APEPKTAKLG PRRESSRPVK PPKKDVPDSQ QHPGPEKSSK
360 370 380 390 400
ISEQLKCCSG ILKEMFAKKH AAYAWPFYKP VDVEALGLHD YCDIIKHPMD
410 420 430 440 450
MSTIKSKLES REYRDAQEFG ADVRLMFSNC YKYNPPDHEV VAMARKLQDV
460 470 480 490 500
FEMRFAKMPD EPEEPVVTVS SPAVPPPTKV VAPPSSSDSS SDSSSDSDSS
510 520 530 540 550
TDDSEEERAQ RLAELQEQLK AVHEQLAALS QPQQNKPKKK EKDKKEKKKE
560 570 580 590 600
KHKKKEEVEE NKKSKTKELP PKKTKKNNSS NSNVSKKEPV PTKTKPPPTY
610 620 630 640 650
ESEEEDKCKP MSYEEKRQLS LDINKLPGEK LGRVVHIIQS REPSLKNSNP
660 670 680 690 700
DEIEIDFETL KPSTLRELER YVTSCLRKKR KPQAEKVDVI AGSSKMKGFS
710 720 730 740 750
SSESESTSES SSSDSEDSET EMAPKSKKKG HTGRDQKKHH HHHHPQMQPA
760 770 780 790 800
PAPVPQQPPP PPQQPPPPPP PQQQQQQPPP PPPPPSMPQQ TAPAMKSSPP
810 820 830 840 850
PFITAQVPVL EPQLPGSVFD PIGHFTQPIL HLPQPELPPH LPQPPEHSTP
860 870 880 890 900
PHLNQHAVVS PPALHNALPQ QPSRPSNRAA ALPPKPTRPP AVSPALAQPP
910 920 930 940 950
LLPQPPMAQP PQVLLEDEEP PAPPLTSMQM QLYLQQLQKV QPPTPLLPSV
960 970 980 990 1000
KVQSQPPPPL PPPPHPSVQQ QQLQPQPPPP PPPQPQPPPQ QQHQPPPRPV
1010 1020 1030 1040 1050
HLPSMPFSAH IQQPPPPPGQ QPTHPPPGQQ PPPPQPAKPQ QVIQHHPSPR
1060 1070 1080 1090 1100
HHKSDPYSAG HLREAPSPLM IHSPQMPQFQ SLTHQSPPQQ NVQPKKQVKG
1110 1120 1130 1140 1150
RAEPQPPGPV MGQGQGCPPA SPAAVPMLSQ ELRPPSVVQP QPLVVVKEEK
1160 1170 1180 1190 1200
IHSPIIRSEP FSTSLRPEPP KHPENIKAPV HLPQRPEMKP VDIGRPVIRP
1210 1220 1230 1240 1250
PEQSAPPPGA PDKDKQKQEP KTPVAPKKDL KIKNMGSWAS LVQKHPTTPS
1260 1270 1280 1290 1300
STAKSSSDSF EHFRRAAREK EEREKALKAQ AEHAEKEKER LRQERMRSRE
1310 1320 1330 1340 1350
DEDALEQARR AHEEARRRQE QQQQQQQQRQ EQQQQQQQAA AVAAASAPQA
1360 1370 1380 1390 1400
QSSQPQSMLD QQRELARKRE QERRRREAMA ATIDMNFQSD LLSIFEENLF
Length:1,400
Mass (Da):155,895
Last modified:July 24, 2013 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3D90F6D3254D5691
GO
Isoform 2 (identifier: Q9ESU6-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     721-723: EMA → GPA
     724-1400: Missing.

Show »
Length:723
Mass (Da):80,605
Checksum:iF1115540F58A56B8
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UH70Q3UH70_MOUSE
Bromodomain-containing protein 4
Brd4
1,401Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
B0V2V6B0V2V6_MOUSE
Bromodomain-containing protein 4
Brd4
557Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
B0V2V8B0V2V8_MOUSE
Bromodomain-containing protein 4
Brd4
745Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
D6RGP2D6RGP2_MOUSE
Bromodomain-containing protein 4
Brd4
146Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A2R8VHW4A0A2R8VHW4_MOUSE
Bromodomain-containing protein 4
Brd4
169Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti188A → T in AAL67834 (PubMed:11997514).Curated1
Sequence conflicti823G → S in AAL67833 (PubMed:11997514).Curated1
Sequence conflicti908A → V in AAG02191 (PubMed:10938129).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting. The information stored in this subsection is used to automatically construct alternative protein sequence(s) for display.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_010904721 – 723EMA → GPA in isoform 2. 1 Publication3
Alternative sequenceiVSP_010905724 – 1400Missing in isoform 2. 1 PublicationAdd BLAST677

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF273217 mRNA Translation: AAG02191.1
AF461395 mRNA Translation: AAL67833.1
AF461396 mRNA Translation: AAL67834.1
CT033751 Genomic DNA No translation available.
CT033755 Genomic DNA No translation available.
CH466640 Genomic DNA Translation: EDL40326.1
CH466640 Genomic DNA Translation: EDL40329.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS37554.1 [Q9ESU6-2]
CCDS50057.1 [Q9ESU6-1]

NCBI Reference Sequences

More...
RefSeqi
NP_065254.3, NM_020508.4 [Q9ESU6-1]
NP_932762.2, NM_198094.2 [Q9ESU6-2]
XP_006524751.1, XM_006524688.1 [Q9ESU6-1]
XP_006524752.1, XM_006524689.2 [Q9ESU6-1]

UniGene gene-oriented nucleotide sequence clusters

More...
UniGenei
Mm.253518

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000120276; ENSMUSP00000112474; ENSMUSG00000024002 [Q9ESU6-2]
ENSMUST00000121285; ENSMUSP00000113070; ENSMUSG00000024002 [Q9ESU6-1]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
57261

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:57261

UCSC genome browser

More...
UCSCi
uc008bwa.3 mouse [Q9ESU6-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF273217 mRNA Translation: AAG02191.1
AF461395 mRNA Translation: AAL67833.1
AF461396 mRNA Translation: AAL67834.1
CT033751 Genomic DNA No translation available.
CT033755 Genomic DNA No translation available.
CH466640 Genomic DNA Translation: EDL40326.1
CH466640 Genomic DNA Translation: EDL40329.1
CCDSiCCDS37554.1 [Q9ESU6-2]
CCDS50057.1 [Q9ESU6-1]
RefSeqiNP_065254.3, NM_020508.4 [Q9ESU6-1]
NP_932762.2, NM_198094.2 [Q9ESU6-2]
XP_006524751.1, XM_006524688.1 [Q9ESU6-1]
XP_006524752.1, XM_006524689.2 [Q9ESU6-1]
UniGeneiMm.253518

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DWWX-ray1.80A347-460[»]
2JNSNMR-A601-683[»]
3JVJX-ray1.55A42-168[»]
3JVKX-ray1.80A42-168[»]
3JVLX-ray1.20A349-464[»]
3JVMX-ray1.20A349-464[»]
3MUKX-ray1.75A42-168[»]
3MULX-ray1.65A42-168[»]
ProteinModelPortaliQ9ESU6
SMRiQ9ESU6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208231, 1 interactor
CORUMiQ9ESU6
DIPiDIP-42181N
IntActiQ9ESU6, 6 interactors
MINTiQ9ESU6
STRINGi10090.ENSMUSP00000113070

Chemistry databases

BindingDBiQ9ESU6
ChEMBLiCHEMBL3085619

PTM databases

iPTMnetiQ9ESU6
PhosphoSitePlusiQ9ESU6

Proteomic databases

EPDiQ9ESU6
PaxDbiQ9ESU6
PRIDEiQ9ESU6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000120276; ENSMUSP00000112474; ENSMUSG00000024002 [Q9ESU6-2]
ENSMUST00000121285; ENSMUSP00000113070; ENSMUSG00000024002 [Q9ESU6-1]
GeneIDi57261
KEGGimmu:57261
UCSCiuc008bwa.3 mouse [Q9ESU6-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
23476
MGIiMGI:1888520 Brd4

Phylogenomic databases

eggNOGiKOG1474 Eukaryota
COG5076 LUCA
GeneTreeiENSGT00940000154549
HOGENOMiHOG000231200
HOVERGENiHBG004896
InParanoidiQ9ESU6
KOiK11722
TreeFamiTF317345

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Brd4 mouse
EvolutionaryTraceiQ9ESU6

Protein Ontology

More...
PROi
PR:Q9ESU6

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000024002 Expressed in 305 organ(s), highest expression level in pituitary gland
CleanExiMM_BRD4
ExpressionAtlasiQ9ESU6 baseline and differential
GenevisibleiQ9ESU6 MM

Family and domain databases

Gene3Di1.20.1270.220, 1 hit
1.20.920.10, 2 hits
InterProiView protein in InterPro
IPR031354 BRD4_CDT
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR027353 NET_dom
IPR038336 NET_sf
PfamiView protein in Pfam
PF17035 BET, 1 hit
PF17105 BRD4_CDT, 1 hit
PF00439 Bromodomain, 2 hits
PRINTSiPR00503 BROMODOMAIN
SMARTiView protein in SMART
SM00297 BROMO, 2 hits
SUPFAMiSSF47370 SSF47370, 2 hits
PROSITEiView protein in PROSITE
PS00633 BROMODOMAIN_1, 1 hit
PS50014 BROMODOMAIN_2, 2 hits
PS51525 NET, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBRD4_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9ESU6
Secondary accession number(s): B0V2V7, Q8VHF7, Q8VHF8
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 24, 2013
Last modified: December 5, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again