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UniProtKB - Q9ESN6 (TRIM2_MOUSE)
Protein
Tripartite motif-containing protein 2
Gene
Trim2
Organism
Mus musculus (Mouse)
Status
Functioni
UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance.
2 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 118 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 121 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 141 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 146 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 23 – 64 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 113 – 154 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- myosin binding Source: UniProtKB
- ubiquitin protein ligase activity Source: MGI
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: MGI
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein polyubiquitination Source: GO_Central
- regulation of neuron apoptotic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Tripartite motif-containing protein 2 (EC:2.3.2.27)Alternative name(s): E3 ubiquitin-protein ligase TRIM2 Neural activity-related RING finger protein RING-type E3 ubiquitin transferase TRIM2Curated |
Gene namesi | Name:Trim2 Synonyms:Kiaa0517, Narf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1933163, Trim2 |
VEuPathDBi | HostDB:ENSMUSG00000027993 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutant mice are indistinguishable from wild type until about 1.5 months of age, when they begin to show intention tremor, followed by gait ataxia. At this stage, neurons exhibit axonal swellings, which consist of the accumulation of disorganized neurofilaments and microtubules, mitochondria and vesicles. In later stages, mutant animals suffer from episodes of spontaneous generalized seizures, a phenotype caused by progressive loss of Purkinje cells through apoptosis. At 4 months of age, retinas in mutant mice display decreased thickness of the inner nuclear layer and a reduced number of ganglionic cells. The outer plexiform layer is also reduced, whereas the size of the photoreceptor layer is not altered.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056196 | 1 – 744 | Tripartite motif-containing protein 2Add BLAST | 744 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | PhosphoserineBy similarity | 1 | |
Modified residuei | 371 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 375 | PhosphoserineCombined sources | 1 | |
Modified residuei | 424 | PhosphoserineCombined sources | 1 | |
Modified residuei | 428 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
RING-type zinc finger-dependent and UBE2D1-dependent autoubiquitination.1 Publication
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | Q9ESN6 |
PaxDbi | Q9ESN6 |
PRIDEi | Q9ESN6 |
ProteomicsDBi | 258979 |
PTM databases
iPTMneti | Q9ESN6 |
PhosphoSitePlusi | Q9ESN6 |
SwissPalmi | Q9ESN6 |
Expressioni
Tissue specificityi
Highly expressed in the cerebellum, hippocampus, retina and spinal cord. In the cerebellum, strongest expression in Purkinje cells and in the deep cerebellar nuclei. In retina, high expression in the ganglionic cell layer, inner nuclear layer and inthe outer plexiform layer. Particularly high expression in the hippocampus, in pyramidal cells of CA1-CA3 hippocampal areas and ingranule cells of the dentate gyrus.2 Publications
Developmental stagei
At 12.5 dpc, expressed in the developing nervous system, particularly in the spinal cord, dorsal rootganglia, hindbrain and midbrain.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027993, Expressed in rostral migratory stream and 336 other tissues |
Genevisiblei | Q9ESN6, MM |
Interactioni
Subunit structurei
Interacts with myosin V; myosin V may not be a substrate for ubiquitination.
Interacts with NEFL.
Interacts with phosphorylated BCL2L11.
2 PublicationsBinary interactionsi
Q9ESN6
With | #Exp. | IntAct |
---|---|---|
Nefl [P08551] | 2 | EBI-8315064,EBI-445199 |
GO - Molecular functioni
- myosin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 219833, 12 interactors |
DIPi | DIP-46254N |
IntActi | Q9ESN6, 1 interactor |
STRINGi | 10090.ENSMUSP00000069922 |
Miscellaneous databases
RNActi | Q9ESN6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 320 – 421 | FilaminAdd BLAST | 102 | |
Repeati | 473 – 516 | NHL 1Add BLAST | 44 | |
Repeati | 520 – 563 | NHL 2Add BLAST | 44 | |
Repeati | 564 – 605 | NHL 3Add BLAST | 42 | |
Repeati | 609 – 652 | NHL 4Add BLAST | 44 | |
Repeati | 656 – 699 | NHL 5Add BLAST | 44 | |
Repeati | 700 – 743 | NHL 6Add BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 432 – 462 | DisorderedSequence analysisAdd BLAST | 31 |
Domaini
The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Sequence similaritiesi
Belongs to the TRIM/RBCC family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 23 – 64 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 113 – 154 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000155905 |
HOGENOMi | CLU_008645_5_0_1 |
InParanoidi | Q9ESN6 |
OrthoDBi | 489543at2759 |
PhylomeDBi | Q9ESN6 |
TreeFami | TF331018 |
Family and domain databases
Gene3Di | 2.120.10.30, 2 hits 2.60.40.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR003649, Bbox_C IPR017868, Filamin/ABP280_repeat-like IPR001298, Filamin/ABP280_rpt IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR001258, NHL_repeat IPR013017, NHL_repeat_subgr IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00630, Filamin, 1 hit PF01436, NHL, 6 hits PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
SMARTi | View protein in SMART SM00502, BBC, 1 hit SM00336, BBOX, 1 hit SM00557, IG_FLMN, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50194, FILAMIN_REPEAT, 1 hit PS51125, NHL, 6 hits PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
Q9ESN6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASEGASIPS PVVRQIDKQF LICSICLERY KNPKVLPCLH TFCERCLQNY
60 70 80 90 100
IPAHSLTLSC PVCRQTSILP EKGVAALQNN FFITNLMDVL QRTPGSNGED
110 120 130 140 150
SSILETVTAV AAGKPLSCPN HDGNVMEFYC QSCETAMCRE CTEGEHAEHP
160 170 180 190 200
TVPLKDVVEQ HKASLQVQLD AVNKRLPEID SALQFISEII HQLTNQKASI
210 220 230 240 250
VDDIHSTFDE LQKTLNVRKS VLLMELEVNY GLKHKVLQSQ LDTLLQGQES
260 270 280 290 300
IKSCSNFTAQ ALNHGTETEV LLVKKQMSEK LNELADQDFP LHPRENDQLD
310 320 330 340 350
FIVETEGLKK SIHNLGTILT TNAVASETVA TGEGLRQTII GQPMSVTITT
360 370 380 390 400
KDKDGELCKT GNAYLTAELS TPDGSVADGE ILDNKNGTYE FLYTVQKEGD
410 420 430 440 450
FTLSLRLYDQ HIRGSPFKLK VIRSADVSPT TEGVKRRVKS PGSGHVKQKA
460 470 480 490 500
VKRPASMYST GKRKENPIED DLIFRVGTKG RNKGEFTNLQ GVAASTSGKI
510 520 530 540 550
LIADSNNQCV QIFSNDGQFK SRFGIRGRSP GQLQRPTGVA VHPSGDIIIA
560 570 580 590 600
DYDNKWVSIF SNDGKFKTKI GSGKLMGPKG VSVDRNGHII VVDNKACCVF
610 620 630 640 650
IFQPNGKIVT RFGSRGNGDR QFAGPHFAAV NSNNEIIITD FHNHSVKVFN
660 670 680 690 700
QEGEFMLKFG SNGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDGSG
710 720 730 740
SFLSYINTSA DPLYGPQGLA LTSDGHVVVA DSGNHCFKVY RYLQ
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QKC6 | E9QKC6_MOUSE | Tripartite motif-containing protein... | Trim2 | 770 | Annotation score: | ||
Q3UHH4 | Q3UHH4_MOUSE | Tripartite motif-containing protein... | Trim2 | 761 | Annotation score: | ||
D3YZJ0 | D3YZJ0_MOUSE | Tripartite motif-containing protein... | Trim2 | 213 | Annotation score: | ||
D3YTN5 | D3YTN5_MOUSE | Tripartite motif-containing protein... | Trim2 | 128 | Annotation score: | ||
D3YY12 | D3YY12_MOUSE | Tripartite motif-containing protein... | Trim2 | 71 | Annotation score: | ||
D3YY69 | D3YY69_MOUSE | Tripartite motif-containing protein... | Trim2 | 191 | Annotation score: |
Sequence cautioni
The sequence BAD90242 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB043550 mRNA Translation: BAB17634.1 AF220017 mRNA Translation: AAG53471.1 AK042752 mRNA Translation: BAC31352.1 AK045410 mRNA Translation: BAC32350.1 AK079415 mRNA Translation: BAC37640.1 AK147275 mRNA Translation: BAE27812.1 AK220343 mRNA Translation: BAD90242.1 Different initiation. BC058961 mRNA Translation: AAH58961.1 |
CCDSi | CCDS71256.1 |
RefSeqi | NP_001258654.1, NM_001271725.1 NP_001258655.1, NM_001271726.1 NP_001258656.1, NM_001271727.1 NP_001258657.1, NM_001271728.1 NP_109631.2, NM_030706.3 XP_017175303.1, XM_017319814.1 |
Genome annotation databases
Ensembli | ENSMUST00000065380; ENSMUSP00000069922; ENSMUSG00000027993 ENSMUST00000107691; ENSMUSP00000103319; ENSMUSG00000027993 ENSMUST00000107692; ENSMUSP00000103320; ENSMUSG00000027993 ENSMUST00000107693; ENSMUSP00000103321; ENSMUSG00000027993 |
GeneIDi | 80890 |
KEGGi | mmu:80890 |
UCSCi | uc008ppx.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB043550 mRNA Translation: BAB17634.1 AF220017 mRNA Translation: AAG53471.1 AK042752 mRNA Translation: BAC31352.1 AK045410 mRNA Translation: BAC32350.1 AK079415 mRNA Translation: BAC37640.1 AK147275 mRNA Translation: BAE27812.1 AK220343 mRNA Translation: BAD90242.1 Different initiation. BC058961 mRNA Translation: AAH58961.1 |
CCDSi | CCDS71256.1 |
RefSeqi | NP_001258654.1, NM_001271725.1 NP_001258655.1, NM_001271726.1 NP_001258656.1, NM_001271727.1 NP_001258657.1, NM_001271728.1 NP_109631.2, NM_030706.3 XP_017175303.1, XM_017319814.1 |
3D structure databases
SMRi | Q9ESN6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 219833, 12 interactors |
DIPi | DIP-46254N |
IntActi | Q9ESN6, 1 interactor |
STRINGi | 10090.ENSMUSP00000069922 |
PTM databases
iPTMneti | Q9ESN6 |
PhosphoSitePlusi | Q9ESN6 |
SwissPalmi | Q9ESN6 |
Proteomic databases
MaxQBi | Q9ESN6 |
PaxDbi | Q9ESN6 |
PRIDEi | Q9ESN6 |
ProteomicsDBi | 258979 |
Protocols and materials databases
Antibodypediai | 27852, 337 antibodies from 31 providers |
DNASUi | 80890 |
Genome annotation databases
Organism-specific databases
CTDi | 23321 |
MGIi | MGI:1933163, Trim2 |
VEuPathDBi | HostDB:ENSMUSG00000027993 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000155905 |
HOGENOMi | CLU_008645_5_0_1 |
InParanoidi | Q9ESN6 |
OrthoDBi | 489543at2759 |
PhylomeDBi | Q9ESN6 |
TreeFami | TF331018 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
BioGRID-ORCSi | 80890, 1 hit in 61 CRISPR screens |
ChiTaRSi | Trim2, mouse |
PROi | PR:Q9ESN6 |
RNActi | Q9ESN6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027993, Expressed in rostral migratory stream and 336 other tissues |
Genevisiblei | Q9ESN6, MM |
Family and domain databases
Gene3Di | 2.120.10.30, 2 hits 2.60.40.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR003649, Bbox_C IPR017868, Filamin/ABP280_repeat-like IPR001298, Filamin/ABP280_rpt IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR001258, NHL_repeat IPR013017, NHL_repeat_subgr IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00630, Filamin, 1 hit PF01436, NHL, 6 hits PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
SMARTi | View protein in SMART SM00502, BBC, 1 hit SM00336, BBOX, 1 hit SM00557, IG_FLMN, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50194, FILAMIN_REPEAT, 1 hit PS51125, NHL, 6 hits PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRIM2_MOUSE | |
Accessioni | Q9ESN6Primary (citable) accession number: Q9ESN6 Secondary accession number(s): Q3UHP5, Q5DU27, Q8C981 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 6, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families