UniProtKB - Q9ESN2 (TRI39_MOUSE)
E3 ubiquitin-protein ligase TRIM39
Trim39
Functioni
E3 ubiquitin-protein ligase (By similarity).
May facilitate apoptosis by inhibiting APC/C-Cdh1-mediated poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1 (By similarity).
Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21 (By similarity).
Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation (By similarity).
By similarityCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 107 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 110 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 129 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 135 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 29 – 70 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 102 – 143 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- identical protein binding Source: MGI
- ubiquitin protein ligase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- innate immune response Source: GO_Central
- mitotic G2 DNA damage checkpoint signaling Source: UniProtKB
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- negative regulation of ubiquitin-dependent protein catabolic process Source: MGI
- positive regulation of apoptotic signaling pathway Source: MGI
- protein stabilization Source: UniProtKB
- protein ubiquitination Source: GO_Central
- regulation of cell cycle G1/S phase transition Source: UniProtKB
- regulation of gene expression Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Apoptosis, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase TRIM39 (EC:2.3.2.27)Alternative name(s): RING finger protein 23 RING-type E3 ubiquitin transferase TRIM39Curated Testis-abundant finger protein Tripartite motif-containing protein 39 |
Gene namesi | Name:Trim39 Synonyms:Rnf23, Tfp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1890659, Trim39 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol By similarity
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion By similarity
Note: Found predominantly in the cytosol. Partial shift from the cytosol to the mitochondria when colocalized with MOAP1. Colocalizes with CDKN1A in the nucleus.By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056258 | 1 – 488 | E3 ubiquitin-protein ligase TRIM39Add BLAST | 488 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
MaxQBi | Q9ESN2 |
PaxDbi | Q9ESN2 |
PRIDEi | Q9ESN2 |
ProteomicsDBi | 298298 [Q9ESN2-1] 298299 [Q9ESN2-2] 298300 [Q9ESN2-3] |
PTM databases
PhosphoSitePlusi | Q9ESN2 |
Interactioni
Subunit structurei
Interacts with MOAP1 (By similarity).
Interacts with CDKN1A (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000109336 |
Miscellaneous databases
RNActi | Q9ESN2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 289 – 484 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 196 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 268 – 307 | Interaction with CDKN1ABy similarityAdd BLAST | 40 | |
Regioni | 359 – 488 | Interaction with CDKN1ABy similarityAdd BLAST | 130 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 181 – 250 | Sequence analysisAdd BLAST | 70 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 29 – 70 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 102 – 143 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
InParanoidi | Q9ESN2 |
OrthoDBi | 423686at2759 |
Family and domain databases
CDDi | cd13745, SPRY_PRY_TRIM39, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035033, PRY/SPRY_TRIM39 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAETSLLEAG ASAASTAAAL ENLQVEASCS VCLEYLKEPV IIECGHNFCK
60 70 80 90 100
ACITRWWEDL ERDFPCPVCR KTSRYRSLRP NRQLGSMVEI AKQLQTVKRK
110 120 130 140 150
IRDESLCSQH HEPLSLFCYE DQEAVCLICA ISHTHRPHTV VPMDDATQEY
160 170 180 190 200
KEKLQKCLEP LEQKLQEITC CKASEEKKPG ELKRLVESRR QQILKEFEEL
210 220 230 240 250
HRRLDEEQQT LLSRLEEEEQ DILQRLRENA AHLGDRRRDL AHLAAEVEGK
260 270 280 290 300
CLQSGFEMLK DVKSTLEKCE KVKTMEVTSV SIELEKNFSN FPRQYFALRK
310 320 330 340 350
ILKQLIADVT LDPETAHPNL VLSEDRKSVK FVETRLRDLP DTPQRFTFYP
360 370 380 390 400
CVLATEGFTS GRHYWEVEVG DKTHWAVGVC RDSVSRKGEL TPLPETGYWR
410 420 430 440 450
VRLWNGDKYA ATTTPFTPLH IKVKPKRVGI FLDYEAGTLS FYNVTDRSHI
460 470 480
YTFTDTFTEK LWPLFYPGIR AGRKNAAPLT IRPPTDWE
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UWI0 | G3UWI0_MOUSE | E3 ubiquitin-protein ligase TRIM39 | Trim39 | 496 | Annotation score: | ||
E9QLN4 | E9QLN4_MOUSE | E3 ubiquitin-protein ligase TRIM39 | Trim39 | 488 | Annotation score: | ||
G3UX29 | G3UX29_MOUSE | E3 ubiquitin-protein ligase TRIM39 | Trim39 | 60 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | A → T in BAC35409 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012063 | 261 – 264 | DVKS → KLCA in isoform 3. 1 Publication | 4 | |
Alternative sequenceiVSP_012064 | 265 – 488 | Missing in isoform 3. 1 PublicationAdd BLAST | 224 | |
Alternative sequenceiVSP_012065 | 307 | A → APLWLLPPA in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046382 mRNA Translation: BAB16375.1 AK053507 mRNA Translation: BAC35409.1 BC031540 mRNA Translation: AAH31540.1 |
CCDSi | CCDS37611.1 [Q9ESN2-1] |
RefSeqi | NP_077788.2, NM_024468.2 XP_006525235.1, XM_006525172.3 |
Genome annotation databases
GeneIDi | 79263 |
KEGGi | mmu:79263 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046382 mRNA Translation: BAB16375.1 AK053507 mRNA Translation: BAC35409.1 BC031540 mRNA Translation: AAH31540.1 |
CCDSi | CCDS37611.1 [Q9ESN2-1] |
RefSeqi | NP_077788.2, NM_024468.2 XP_006525235.1, XM_006525172.3 |
3D structure databases
BMRBi | Q9ESN2 |
SMRi | Q9ESN2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000109336 |
PTM databases
PhosphoSitePlusi | Q9ESN2 |
Proteomic databases
MaxQBi | Q9ESN2 |
PaxDbi | Q9ESN2 |
PRIDEi | Q9ESN2 |
ProteomicsDBi | 298298 [Q9ESN2-1] 298299 [Q9ESN2-2] 298300 [Q9ESN2-3] |
Protocols and materials databases
DNASUi | 79263 |
Genome annotation databases
GeneIDi | 79263 |
KEGGi | mmu:79263 |
Organism-specific databases
CTDi | 56658 |
MGIi | MGI:1890659, Trim39 |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
InParanoidi | Q9ESN2 |
OrthoDBi | 423686at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 79263, 1 hit in 65 CRISPR screens |
ChiTaRSi | Trim39, mouse |
PROi | PR:Q9ESN2 |
RNActi | Q9ESN2, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd13745, SPRY_PRY_TRIM39, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR035033, PRY/SPRY_TRIM39 IPR003877, SPRY_dom IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF00622, SPRY, 1 hit PF00643, zf-B_box, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI39_MOUSE | |
Accessioni | Q9ESN2Primary (citable) accession number: Q9ESN2 Secondary accession number(s): Q8BPR5, Q8K0F7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families