UniProtKB - Q9ESE2 (TNF11_RAT)
Protein
Tumor necrosis factor ligand superfamily member 11
Gene
Tnfsf11
Organism
Rattus norvegicus (Rat)
Status
Functioni
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.By similarity
GO - Molecular functioni
- cytokine activity Source: RGD
- identical protein binding Source: RGD
- tumor necrosis factor receptor binding Source: InterPro
- tumor necrosis factor receptor superfamily binding Source: RGD
GO - Biological processi
- activation of JUN kinase activity Source: RGD
- animal organ morphogenesis Source: RGD
- bone development Source: RGD
- bone resorption Source: RGD
- calcium ion homeostasis Source: RGD
- calcium-mediated signaling Source: UniProtKB
- cellular response to leukemia inhibitory factor Source: RGD
- cytokine-mediated signaling pathway Source: RGD
- ERK1 and ERK2 cascade Source: RGD
- immune response Source: InterPro
- mammary gland alveolus development Source: RGD
- mammary gland epithelial cell proliferation Source: RGD
- monocyte chemotaxis Source: RGD
- ossification Source: RGD
- osteoclast development Source: RGD
- osteoclast differentiation Source: RGD
- osteoclast proliferation Source: RGD
- paracrine signaling Source: RGD
- positive regulation of bone resorption Source: RGD
- positive regulation of corticotropin-releasing hormone secretion Source: RGD
- positive regulation of DNA-binding transcription factor activity Source: RGD
- positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling Source: RGD
- positive regulation of fever generation by positive regulation of prostaglandin secretion Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of homotypic cell-cell adhesion Source: RGD
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: RGD
- positive regulation of intracellular signal transduction Source: RGD
- positive regulation of JNK cascade Source: RGD
- positive regulation of MAP kinase activity Source: RGD
- positive regulation of NF-kappaB transcription factor activity Source: RGD
- positive regulation of osteoclast development Source: RGD
- positive regulation of osteoclast differentiation Source: RGD
- positive regulation of phosphorylation Source: RGD
- positive regulation of protein kinase B signaling Source: RGD
- positive regulation of T cell activation Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of actin binding Source: RGD
- regulation of osteoclast differentiation Source: RGD
- response to immobilization stress Source: RGD
- response to radiation Source: RGD
- TNFSF11-mediated signaling pathway Source: RGD
- tooth eruption Source: RGD
- tumor necrosis factor-mediated signaling pathway Source: RGD
Keywordsi
Molecular function | Cytokine, Developmental protein, Receptor |
Biological process | Differentiation |
Names & Taxonomyi
Protein namesi | Recommended name: Tumor necrosis factor ligand superfamily member 11Alternative name(s): Osteoclast differentiation factor Short name: ODF Osteoprotegerin ligand Short name: OPGL Receptor activator of nuclear factor kappa-B ligand Short name: RANKL TNF-related activation-induced cytokine Short name: TRANCE CD_antigen: CD254 Cleaved into the following 2 chains: |
Gene namesi | Name:Tnfsf11 Synonyms:Opgl, Rankl, Trance |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620784, Tnfsf11 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB-KW
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 47 | CytoplasmicSequence analysisAdd BLAST | 47 | |
Transmembranei | 48 – 68 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 69 – 318 | ExtracellularSequence analysisAdd BLAST | 250 |
Keywords - Cellular componenti
Cell membrane, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000034518 | 1 – 318 | Tumor necrosis factor ligand superfamily member 11, membrane formAdd BLAST | 318 | |
ChainiPRO_0000034519 | 141 – 318 | Tumor necrosis factor ligand superfamily member 11, soluble formAdd BLAST | 178 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 199 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 264 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
The soluble form derives from the membrane form by proteolytic processing.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 140 – 141 | CleavageBy similarity | 2 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9ESE2 |
PTM databases
GlyGeni | Q9ESE2, 2 sites |
Expressioni
Tissue specificityi
Highly expressed in thymus and bone tissues.
Interactioni
Subunit structurei
Homotrimer.
Interacts with TNFRSF11A and TNFRSF11B.
Interacts with FBN1 (via N-terminal domain) in a Ca(+2)-dependent manner.
By similarityGO - Molecular functioni
- cytokine activity Source: RGD
- identical protein binding Source: RGD
- tumor necrosis factor receptor binding Source: InterPro
- tumor necrosis factor receptor superfamily binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013210 |
Family & Domainsi
Sequence similaritiesi
Belongs to the tumor necrosis factor family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502R8MX, Eukaryota |
InParanoidi | Q9ESE2 |
OrthoDBi | 1404113at2759 |
PhylomeDBi | Q9ESE2 |
Family and domain databases
CDDi | cd00184, TNF, 1 hit |
Gene3Di | 2.60.120.40, 1 hit |
InterProi | View protein in InterPro IPR006052, TNF_dom IPR017355, TNF_ligand_10/11 IPR008983, Tumour_necrosis_fac-like_dom |
Pfami | View protein in Pfam PF00229, TNF, 1 hit |
PIRSFi | PIRSF038013, TNF10_TNF11, 1 hit |
SMARTi | View protein in SMART SM00207, TNF, 1 hit |
SUPFAMi | SSF49842, SSF49842, 1 hit |
PROSITEi | View protein in PROSITE PS50049, TNF_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9ESE2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRANRDYGK YLRGSEEMGS CPGVPHEGPL HPAPSAPAPA PPPAASRFMF
60 70 80 90 100
LALLGLGLGQ VVCSIALFLY FRAQMDPNRI SEDSTRCFYR ILRLRENTGL
110 120 130 140 150
QDSTLESEDT EALPDSCRRM KQAFQGAVQR ELQHIVGPQR FSGVPAMMEG
160 170 180 190 200
SWLDVARRGK PEAQPFAHLT INAADIPSGS HKVSLSSWYH DRGWAKISNM
210 220 230 240 250
TLSNGKLRVN QDGFYYLYAN ICFRHHETSG SVPADYLQLM VYVVKTSIKI
260 270 280 290 300
PSSHNLMKGG STKNWSGNSE FHFYSINVGG FFKLRAGEEI SVQVSNPSLL
310
DPDQDATYFG AFKVQDID
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V794 | G3V794_RAT | Tumor necrosis factor ligand superf... | LOC103689974 Tnfsf11, rCG_36849 | 318 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 317 | I → M in AAL23963 (PubMed:11804028).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF187319 mRNA Translation: AAG17031.1 AF425669 mRNA Translation: AAL23963.1 |
RefSeqi | NP_476490.1, NM_057149.1 |
Genome annotation databases
GeneIDi | 117516 |
KEGGi | rno:117516 |
UCSCi | RGD:620784, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF187319 mRNA Translation: AAG17031.1 AF425669 mRNA Translation: AAL23963.1 |
RefSeqi | NP_476490.1, NM_057149.1 |
3D structure databases
SMRi | Q9ESE2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013210 |
PTM databases
GlyGeni | Q9ESE2, 2 sites |
Proteomic databases
PaxDbi | Q9ESE2 |
Genome annotation databases
GeneIDi | 117516 |
KEGGi | rno:117516 |
UCSCi | RGD:620784, rat |
Organism-specific databases
CTDi | 8600 |
RGDi | 620784, Tnfsf11 |
Phylogenomic databases
eggNOGi | ENOG502R8MX, Eukaryota |
InParanoidi | Q9ESE2 |
OrthoDBi | 1404113at2759 |
PhylomeDBi | Q9ESE2 |
Miscellaneous databases
PROi | PR:Q9ESE2 |
Family and domain databases
CDDi | cd00184, TNF, 1 hit |
Gene3Di | 2.60.120.40, 1 hit |
InterProi | View protein in InterPro IPR006052, TNF_dom IPR017355, TNF_ligand_10/11 IPR008983, Tumour_necrosis_fac-like_dom |
Pfami | View protein in Pfam PF00229, TNF, 1 hit |
PIRSFi | PIRSF038013, TNF10_TNF11, 1 hit |
SMARTi | View protein in SMART SM00207, TNF, 1 hit |
SUPFAMi | SSF49842, SSF49842, 1 hit |
PROSITEi | View protein in PROSITE PS50049, TNF_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TNF11_RAT | |
Accessioni | Q9ESE2Primary (citable) accession number: Q9ESE2 Secondary accession number(s): Q91ZI9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 6, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families